LOCUS       Exported     3092 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3092
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1105..1152
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2980..2996
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2997..3028
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0831"
                     /note=""
                     /translation="MDNKDIYVFGLSASQQLAKEVCHFLGVEQKVVKTTRFADGEILVESIDSVRGKEIYVIQSTSMPVNENLMELLIAIDAFKRGSAEKINVVIPYYGYARQDRKAKGRQPITAKLVADLLTKAGADRVIVFDIHSTQTMGFFDMPMDNFHTSQSLANEIVDTIIREKFDPEKCILVSPDYGGLNRVHKVDSYTANMTNGIAVIGKRRPEPNKAEVEFVLGDIEGRTCFIIDDMIDTGGTIISGAKALKANGAKDVYIFACHGLFNGPAKERMTQAIKEGIVKNVVVTNTVEIPQERQFEGLKIVSVAPLLANMIKESQEHHSLTEVYNKNKDEIQLKIQDFMNHKN"
     primer_bind     complement(1157..1173)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1240..1828)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1999..2859)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2860..2964)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0831"
                     /note=""
                     /translation="MDNKDIYVFGLSASQQLAKEVCHFLGVEQKVVKTTRFADGEILVESIDSVRGKEIYVIQSTSMPVNENLMELLIAIDAFKRGSAEKINVVIPYYGYARQDRKAKGRQPITAKLVADLLTKAGADRVIVFDIHSTQTMGFFDMPMDNFHTSQSLANEIVDTIIREKFDPEKCILVSPDYGGLNRVHKVDSYTANMTNGIAVIGKRRPEPNKAEVEFVLGDIEGRTCFIIDDMIDTGGTIISGAKALKANGAKDVYIFACHGLFNGPAKERMTQAIKEGIVKNVVVTNTVEIPQERQFEGLKIVSVAPLLANMIKESQEHHSLTEVYNKNKDEIQLKIQDFMNHKN"
ORIGIN
        1 ATGGACAACA AGGACATCTA CGTGTTCGGT TTAAGTGCTA GCCAACAGCT GGCGAAGGAA
       61 GTATGTCATT TTTTAGGTGT CGAACAAAAG GTTGTAAAAA CAACTCGTTT CGCTGATGGG
      121 GAAATTCTGG TTGAATCAAT CGATTCGGTT CGTGGAAAGG AAATCTACGT AATTCAAAGT
      181 ACATCGATGC CGGTAAACGA AAATTTAATG GAACTGCTTA TTGCGATCGA CGCTTTCAAG
      241 CGTGGAAGCG CGGAAAAGAT TAACGTGGTA ATTCCCTATT ACGGCTACGC ACGCCAGGAT
      301 CGCAAGGCTA AGGGCCGCCA ACCTATTACA GCGAAGTTGG TCGCTGATTT ATTGACAAAA
      361 GCCGGGGCAG ATCGCGTCAT CGTGTTTGAT ATTCACAGTA CTCAAACCAT GGGTTTCTTC
      421 GATATGCCGA TGGACAACTT TCACACATCC CAATCCCTGG CGAATGAGAT CGTTGATACT
      481 ATCATCCGCG AGAAGTTTGA TCCGGAAAAG TGCATCTTGG TTTCGCCAGA CTATGGTGGT
      541 TTAAACCGCG TGCATAAAGT CGACTCTTAT ACCGCAAACA TGACAAACGG TATTGCTGTC
      601 ATCGGCAAGC GCCGTCCCGA ACCAAATAAA GCTGAGGTAG AGTTTGTCTT AGGTGACATT
      661 GAGGGGCGCA CATGTTTCAT CATTGACGAC ATGATTGACA CAGGAGGCAC GATTATTTCT
      721 GGGGCTAAGG CACTGAAGGC AAACGGGGCG AAAGATGTCT ACATCTTCGC TTGTCATGGC
      781 CTTTTCAACG GGCCCGCAAA AGAGCGCATG ACGCAGGCTA TCAAAGAGGG CATCGTAAAA
      841 AATGTCGTTG TTACTAACAC AGTAGAAATC CCGCAAGAGC GCCAATTCGA GGGGTTGAAG
      901 ATCGTATCTG TAGCTCCCCT GCTTGCAAAT ATGATTAAGG AAAGCCAGGA GCACCATTCT
      961 CTTACCGAGG TCTACAATAA GAACAAAGAT GAGATTCAAT TAAAAATTCA AGATTTTATG
     1021 AACCACAAAA ACTGAAGAGC TTAGAGACCG GTACTAGTTC CTGCAGGGCT CGAGGTGTCA
     1081 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1141 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1201 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1261 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1321 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1381 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1441 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1501 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1561 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1621 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1681 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1741 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1801 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
     1861 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT
     1921 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA
     1981 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG
     2041 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA
     2101 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC
     2161 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC
     2221 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC
     2281 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC
     2341 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC
     2401 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT
     2461 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC
     2521 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG
     2581 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG
     2641 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT
     2701 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC
     2761 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA
     2821 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA
     2881 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     2941 AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC
     3001 AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGAATTCGC GGCCGCTTCT
     3061 AGAGGCTCTT CGTAATTACG AAGAGGTCTC TA
//