LOCUS Exported 3092 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3092 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1105..1152 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2980..2996 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2997..3028 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 1..0 /label="MMSYN1_0831" /note="" /translation="MDNKDIYVFGLSASQQLAKEVCHFLGVEQKVVKTTRFADGEILVESIDSVRGKEIYVIQSTSMPVNENLMELLIAIDAFKRGSAEKINVVIPYYGYARQDRKAKGRQPITAKLVADLLTKAGADRVIVFDIHSTQTMGFFDMPMDNFHTSQSLANEIVDTIIREKFDPEKCILVSPDYGGLNRVHKVDSYTANMTNGIAVIGKRRPEPNKAEVEFVLGDIEGRTCFIIDDMIDTGGTIISGAKALKANGAKDVYIFACHGLFNGPAKERMTQAIKEGIVKNVVVTNTVEIPQERQFEGLKIVSVAPLLANMIKESQEHHSLTEVYNKNKDEIQLKIQDFMNHKN" primer_bind complement(1157..1173) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1240..1828) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1999..2859) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2860..2964) /label="bla promoter" /note="ampicillin resistance gene promoter" CDS complement(1..0) /label="MMSYN1_0831" /note="" /translation="MDNKDIYVFGLSASQQLAKEVCHFLGVEQKVVKTTRFADGEILVESIDSVRGKEIYVIQSTSMPVNENLMELLIAIDAFKRGSAEKINVVIPYYGYARQDRKAKGRQPITAKLVADLLTKAGADRVIVFDIHSTQTMGFFDMPMDNFHTSQSLANEIVDTIIREKFDPEKCILVSPDYGGLNRVHKVDSYTANMTNGIAVIGKRRPEPNKAEVEFVLGDIEGRTCFIIDDMIDTGGTIISGAKALKANGAKDVYIFACHGLFNGPAKERMTQAIKEGIVKNVVVTNTVEIPQERQFEGLKIVSVAPLLANMIKESQEHHSLTEVYNKNKDEIQLKIQDFMNHKN" ORIGIN 1 ATGGACAACA AGGACATCTA CGTGTTCGGT TTAAGTGCTA GCCAACAGCT GGCGAAGGAA 61 GTATGTCATT TTTTAGGTGT CGAACAAAAG GTTGTAAAAA CAACTCGTTT CGCTGATGGG 121 GAAATTCTGG TTGAATCAAT CGATTCGGTT CGTGGAAAGG AAATCTACGT AATTCAAAGT 181 ACATCGATGC CGGTAAACGA AAATTTAATG GAACTGCTTA TTGCGATCGA CGCTTTCAAG 241 CGTGGAAGCG CGGAAAAGAT TAACGTGGTA ATTCCCTATT ACGGCTACGC ACGCCAGGAT 301 CGCAAGGCTA AGGGCCGCCA ACCTATTACA GCGAAGTTGG TCGCTGATTT ATTGACAAAA 361 GCCGGGGCAG ATCGCGTCAT CGTGTTTGAT ATTCACAGTA CTCAAACCAT GGGTTTCTTC 421 GATATGCCGA TGGACAACTT TCACACATCC CAATCCCTGG CGAATGAGAT CGTTGATACT 481 ATCATCCGCG AGAAGTTTGA TCCGGAAAAG TGCATCTTGG TTTCGCCAGA CTATGGTGGT 541 TTAAACCGCG TGCATAAAGT CGACTCTTAT ACCGCAAACA TGACAAACGG TATTGCTGTC 601 ATCGGCAAGC GCCGTCCCGA ACCAAATAAA GCTGAGGTAG AGTTTGTCTT AGGTGACATT 661 GAGGGGCGCA CATGTTTCAT CATTGACGAC ATGATTGACA CAGGAGGCAC GATTATTTCT 721 GGGGCTAAGG CACTGAAGGC AAACGGGGCG AAAGATGTCT ACATCTTCGC TTGTCATGGC 781 CTTTTCAACG GGCCCGCAAA AGAGCGCATG ACGCAGGCTA TCAAAGAGGG CATCGTAAAA 841 AATGTCGTTG TTACTAACAC AGTAGAAATC CCGCAAGAGC GCCAATTCGA GGGGTTGAAG 901 ATCGTATCTG TAGCTCCCCT GCTTGCAAAT ATGATTAAGG AAAGCCAGGA GCACCATTCT 961 CTTACCGAGG TCTACAATAA GAACAAAGAT GAGATTCAAT TAAAAATTCA AGATTTTATG 1021 AACCACAAAA ACTGAAGAGC TTAGAGACCG GTACTAGTTC CTGCAGGGCT CGAGGTGTCA 1081 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG 1141 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT 1201 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC 1261 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 1321 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 1381 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 1441 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 1501 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 1561 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 1621 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 1681 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 1741 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 1801 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 1861 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 1921 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 1981 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 2041 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 2101 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC 2161 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 2221 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 2281 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 2341 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 2401 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 2461 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 2521 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 2581 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 2641 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 2701 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 2761 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 2821 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 2881 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 2941 AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC 3001 AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGAATTCGC GGCCGCTTCT 3061 AGAGGCTCTT CGTAATTACG AAGAGGTCTC TA //