LOCUS       Exported     2618 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2618
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      631..678
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2506..2522
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2523..2554
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0832"
                     /note="The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis."
                     /translation="MKVIIGLGNIGKEYEKTRHNAGFIAIDLLLEKYNYSSVKQEFNSLIYTTMINNQKVLLVKPLTFMNNSGIAVRQIINFYKIDLNDLIIIHDDKDLNISRIQFKKDGSSAGHNGIKSIINNLQTQNFYRLRIGVNQVPKEWKIVDWVLSKFSDEELNLLKQSFIDKIEFINDFTNNKTFIYLMNKYN"
     primer_bind     complement(683..699)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(766..1354)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1525..2385)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2386..2490)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0832"
                     /note="The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis."
                     /translation="MKVIIGLGNIGKEYEKTRHNAGFIAIDLLLEKYNYSSVKQEFNSLIYTTMINNQKVLLVKPLTFMNNSGIAVRQIINFYKIDLNDLIIIHDDKDLNISRIQFKKDGSSAGHNGIKSIINNLQTQNFYRLRIGVNQVPKEWKIVDWVLSKFSDEELNLLKQSFIDKIEFINDFTNNKTFIYLMNKYN"
ORIGIN
        1 ATGAAGGTAA TTATCGGGCT GGGTAATATT GGTAAAGAAT ATGAAAAGAC GCGTCATAAC
       61 GCGGGGTTTA TCGCAATCGA TCTGCTGTTG GAAAAGTACA ATTATTCCTC TGTCAAGCAG
      121 GAATTCAACT CCCTGATCTA CACTACGATG ATTAATAATC AAAAGGTATT GTTAGTCAAA
      181 CCCCTTACGT TTATGAATAA TTCGGGTATT GCGGTACGTC AAATCATTAA CTTTTACAAG
      241 ATCGACCTTA ATGACTTAAT CATCATCCAT GATGATAAGG ACTTGAATAT TTCGCGTATT
      301 CAGTTCAAAA AAGATGGTTC CTCCGCTGGT CATAACGGGA TCAAATCGAT CATTAATAAT
      361 TTGCAAACGC AGAATTTCTA CCGCTTACGC ATTGGCGTCA ACCAAGTGCC TAAAGAGTGG
      421 AAGATTGTTG ACTGGGTATT GTCCAAATTT TCTGACGAGG AATTGAATTT ACTGAAGCAA
      481 TCATTCATTG ATAAAATTGA GTTCATTAAC GATTTCACAA ACAACAAGAC GTTCATTTAC
      541 CTTATGAATA AATACAACTG AAGAGCTTAG AGACCGGTAC TAGTTCCTGC AGGGCTCGAG
      601 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
      661 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
      721 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
      781 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
      841 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
      901 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
      961 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1021 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1081 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1141 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1201 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1261 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1321 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     1381 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     1441 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     1501 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     1561 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     1621 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC
     1681 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     1741 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     1801 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     1861 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     1921 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     1981 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     2041 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     2101 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     2161 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     2221 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     2281 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     2341 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     2401 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     2461 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA
     2521 GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGA ATTCGCGGCC
     2581 GCTTCTAGAG GCTCTTCGTA ATTACGAAGA GGTCTCTA
//