LOCUS       Exported     3383 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3383
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1396..1443
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3271..3287
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3288..3319
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0837"
                     /note="CysRS: Cysteinyl-tRNA synthetase"
                     /translation="MQLYDSLSKTKKNLNKKTINLYCCGPTVYNYIHIGNARPVLLVDVLIRYLKSRNIKVNYLQNITDIDDKIILKALDNNLNELEVSQKYTTAYLEDLKSLNINQPDKIILISQKMNEMIDFIKNLVDINAAYVLDGDVYFDIKKYENEYCKLSGYKLDELISGKRIEIDSKKHYSLDFSLWKKTDIGIKWNSVFGLGRPGWHTECVLLIDEYFNHQTIDIHVGGIDLKFPHHENERIQFIAKNNKELAHIWLHNGHLQINDEKMSKSLGNVILVRDFNKQHNKNTLRWIFLTTNYTQPLNISDDLIYQANKFFEKLTNLSKKTIQFIIKNDLKIQSINSSEYINKFNEYMEDDLNTSLVLSLIDLLIKQINKDIVDKNLDNFNLLIGSLNYILDVLGFNNVFNYKFDNKTKELFLKWQELVKNKEFNKADIIRNKLIEQGIL"
     primer_bind     complement(1448..1464)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1531..2119)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2290..3150)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3151..3255)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0837"
                     /note="CysRS: Cysteinyl-tRNA synthetase"
                     /translation="MQLYDSLSKTKKNLNKKTINLYCCGPTVYNYIHIGNARPVLLVDVLIRYLKSRNIKVNYLQNITDIDDKIILKALDNNLNELEVSQKYTTAYLEDLKSLNINQPDKIILISQKMNEMIDFIKNLVDINAAYVLDGDVYFDIKKYENEYCKLSGYKLDELISGKRIEIDSKKHYSLDFSLWKKTDIGIKWNSVFGLGRPGWHTECVLLIDEYFNHQTIDIHVGGIDLKFPHHENERIQFIAKNNKELAHIWLHNGHLQINDEKMSKSLGNVILVRDFNKQHNKNTLRWIFLTTNYTQPLNISDDLIYQANKFFEKLTNLSKKTIQFIIKNDLKIQSINSSEYINKFNEYMEDDLNTSLVLSLIDLLIKQINKDIVDKNLDNFNLLIGSLNYILDVLGFNNVFNYKFDNKTKELFLKWQELVKNKEFNKADIIRNKLIEQGIL"
ORIGIN
        1 ATGCAGCTTT ATGACTCCTT GAGCAAGACA AAGAAAAACT TAAACAAAAA GACTATCAAC
       61 TTGTACTGTT GTGGACCTAC AGTCTATAAC TATATTCATA TTGGGAATGC CCGTCCGGTA
      121 CTTCTTGTTG ACGTCTTAAT CCGCTATTTG AAATCACGTA ACATTAAGGT CAATTACTTG
      181 CAAAATATCA CGGACATTGA TGACAAAATT ATTCTTAAGG CGTTAGACAA CAATTTAAAC
      241 GAGCTTGAGG TTTCCCAAAA GTACACAACC GCCTATCTGG AGGACCTGAA AAGCCTGAAC
      301 ATTAACCAAC CCGACAAGAT CATCCTGATC AGTCAAAAGA TGAATGAAAT GATTGATTTC
      361 ATCAAAAATT TAGTAGACAT CAATGCGGCC TACGTGTTGG ATGGGGATGT GTACTTCGAT
      421 ATCAAAAAGT ATGAAAATGA GTACTGTAAG CTTAGTGGGT ATAAGCTTGA TGAACTTATT
      481 TCTGGGAAAC GTATTGAAAT CGACTCAAAG AAACATTACA GCCTTGATTT CAGTCTGTGG
      541 AAAAAGACGG ACATCGGGAT TAAATGGAAC AGCGTATTCG GATTGGGGCG TCCGGGGTGG
      601 CACACCGAAT GCGTTTTACT TATCGACGAA TATTTCAACC ACCAGACAAT CGACATTCAT
      661 GTAGGAGGTA TTGACCTGAA GTTTCCTCAC CACGAAAATG AGCGCATCCA ATTTATTGCG
      721 AAAAATAATA AGGAGTTAGC ACATATTTGG TTACACAACG GACATCTGCA AATTAATGAT
      781 GAGAAGATGA GTAAGTCCCT TGGCAACGTC ATTCTGGTCC GCGATTTTAA TAAGCAGCAT
      841 AATAAAAATA CTTTACGTTG GATTTTCTTA ACAACGAATT ACACCCAGCC TCTGAACATC
      901 TCGGATGACC TTATCTATCA GGCTAACAAG TTTTTTGAAA AATTGACTAA CTTGAGCAAA
      961 AAGACTATTC AATTCATCAT TAAGAACGAT CTTAAGATTC AGTCCATTAA TTCTAGCGAA
     1021 TACATTAATA AATTTAATGA ATACATGGAG GACGACCTTA ATACGAGTCT GGTGCTGAGT
     1081 CTTATTGACT TACTTATTAA ACAGATTAAT AAGGACATCG TAGACAAAAA TTTAGATAAC
     1141 TTCAATTTGT TAATTGGCTC CTTGAATTAT ATTTTGGACG TCCTGGGTTT CAATAACGTG
     1201 TTCAACTATA AATTCGATAA CAAGACTAAA GAGTTGTTCC TGAAGTGGCA AGAACTGGTC
     1261 AAAAACAAAG AGTTCAACAA AGCGGACATC ATCCGCAACA AATTGATCGA ACAGGGGATT
     1321 CTGTGAAGAG CTTAGAGACC GGTACTAGTT CCTGCAGGGC TCGAGGTGTC AATCGTCGGA
     1381 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT
     1441 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT
     1501 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     1561 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     1621 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     1681 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     1741 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     1801 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     1861 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     1921 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT
     1981 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     2041 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     2101 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
     2161 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA
     2221 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG
     2281 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG
     2341 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC
     2401 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC
     2461 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC
     2521 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG
     2581 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT
     2641 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG
     2701 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT
     2761 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG
     2821 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG
     2881 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT
     2941 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT
     3001 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC
     3061 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT
     3121 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     3181 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     3241 AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC
     3301 CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGAATTCG CGGCCGCTTC TAGAGGCTCT
     3361 TCGTAATTAC GAAGAGGTCT CTA
//