LOCUS       Exported     2699 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2699
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      712..759
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     2587..2603
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2604..2635
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0840"
                     /note="Participates in transcription elongation, termination and antitermination."
                     /translation="MTYEEIKQLEDEILEAKGQWFVISCQTGHEEKVLGDLQQKIKSASIEDEVFSIKISKANLVSKSGKSSIKNKFPGYIFINMIMSEKAWFLIRNTPGVTGFIGSSGRGAKPSPLTTEETLNMLVPNLEEIEQAHEQEQQEENLKNEAVNKKELFTANFKVGDVVRVKSGIHENEEGTVKDMDFSKGVAFVAIEMFGRWTTLEVSFKNVEPIKEY"
     primer_bind     complement(764..780)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(847..1435)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1606..2466)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2467..2571)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0840"
                     /note="Participates in transcription elongation, termination and antitermination."
                     /translation="MTYEEIKQLEDEILEAKGQWFVISCQTGHEEKVLGDLQQKIKSASIEDEVFSIKISKANLVSKSGKSSIKNKFPGYIFINMIMSEKAWFLIRNTPGVTGFIGSSGRGAKPSPLTTEETLNMLVPNLEEIEQAHEQEQQEENLKNEAVNKKELFTANFKVGDVVRVKSGIHENEEGTVKDMDFSKGVAFVAIEMFGRWTTLEVSFKNVEPIKEY"
ORIGIN
        1 ATGACGTATG AAGAGATCAA ACAATTAGAG GACGAAATTC TTGAAGCAAA AGGCCAGTGG
       61 TTCGTAATTA GCTGTCAAAC CGGACATGAG GAAAAAGTTT TGGGTGACCT TCAACAGAAG
      121 ATTAAAAGTG CATCAATCGA GGACGAGGTT TTTTCAATCA AAATTTCAAA GGCTAATCTG
      181 GTATCTAAAA GTGGCAAATC CTCTATCAAG AATAAATTCC CGGGGTATAT TTTCATCAAC
      241 ATGATTATGA GCGAAAAGGC CTGGTTTCTG ATCCGCAACA CCCCGGGTGT AACCGGATTT
      301 ATCGGATCGA GCGGACGCGG TGCTAAGCCC TCGCCCCTGA CGACTGAAGA AACGCTGAAC
      361 ATGCTTGTCC CTAACTTGGA GGAGATTGAA CAAGCTCATG AGCAGGAGCA GCAAGAAGAA
      421 AACTTGAAGA ACGAGGCAGT AAATAAGAAG GAATTGTTCA CCGCTAATTT TAAGGTAGGG
      481 GATGTCGTCC GCGTTAAATC TGGTATTCAC GAAAACGAGG AGGGCACTGT TAAAGACATG
      541 GACTTCTCGA AAGGGGTAGC GTTTGTAGCC ATCGAAATGT TTGGCCGTTG GACGACCTTA
      601 GAAGTTTCTT TCAAGAACGT AGAACCGATT AAGGAGTACT GAAGAGCTTA GAGACCGGTA
      661 CTAGTTCCTG CAGGGCTCGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
      721 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
      781 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
      841 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
      901 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
      961 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1021 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1081 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1141 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     1201 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     1261 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     1321 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     1381 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
     1441 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT
     1501 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG
     1561 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
     1621 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
     1681 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
     1741 ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG
     1801 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG
     1861 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
     1921 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA
     1981 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG
     2041 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC
     2101 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA
     2161 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
     2221 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG
     2281 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC
     2341 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC
     2401 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT
     2461 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
     2521 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC
     2581 CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC
     2641 AGGTGGAGTG AATTCGCGGC CGCTTCTAGA GGCTCTTCGT AATTACGAAG AGGTCTCTA
//