LOCUS       Exported     3824 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3824
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1837..1884
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3712..3728
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3729..3760
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /note=""
                     /translation="MHIKVENTEMNNFNSNIKKKKRLKMLSSFSILLLIMLVLMLVSWILYWSKTKTDLVKTISFNDWKYDPILSPIYNAWTSKYPNISAGNSQTWIDFMNSNSSLGWVYNSHGWIKDSYTIQHSGDAIFNGLAPIQPIGIIDVIYAPIKGFVLKSNIIIFTISIGAFLYILVSTKALEGLSQAIIAKLKGKEAFAIIPLMLFFSIFGTVEGFAEETLGFYMIFIPIMLMAGFDVFTGVLILMVGAGTGVIGSTVNPFTIPIAVSAINSGIDASTAKLTIGDGLVWRIICWLILTSFSTTFTLLYALKVKKNPSKSVTFSTLEGDKEFFLAHVSKTIKLDWKKKVSLVAFAISFLVMIFYLVGWDSIFNNTKMADQAIWIKKNIPYLTALIPGWGNGDLDNVAAFFLLASITLAIINSIGEATFIKKWFEGASDILSVAFIIATAAGVGYILVQTNLQSLFVKGILSSIGGINNQTAKVIVLFIVFIPLAFLIPSSSGFATTIFPLLAKSLVDSKTNQLQAYASSGSIMAFTFAIGLVNLITPTSGVVMGACSLSRMSYAKYLKAMLPIISYLFILCFILLLIGGALPDSIS"
                     /label="MMSYN1_0870"
     primer_bind     complement(1889..1905)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1972..2560)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2731..3591)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3592..3696)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0870"
                     /note=""
                     /translation="MHIKVENTEMNNFNSNIKKKKRLKMLSSFSILLLIMLVLMLVSWILYWSKTKTDLVKTISFNDWKYDPILSPIYNAWTSKYPNISAGNSQTWIDFMNSNSSLGWVYNSHGWIKDSYTIQHSGDAIFNGLAPIQPIGIIDVIYAPIKGFVLKSNIIIFTISIGAFLYILVSTKALEGLSQAIIAKLKGKEAFAIIPLMLFFSIFGTVEGFAEETLGFYMIFIPIMLMAGFDVFTGVLILMVGAGTGVIGSTVNPFTIPIAVSAINSGIDASTAKLTIGDGLVWRIICWLILTSFSTTFTLLYALKVKKNPSKSVTFSTLEGDKEFFLAHVSKTIKLDWKKKVSLVAFAISFLVMIFYLVGWDSIFNNTKMADQAIWIKKNIPYLTALIPGWGNGDLDNVAAFFLLASITLAIINSIGEATFIKKWFEGASDILSVAFIIATAAGVGYILVQTNLQSLFVKGILSSIGGINNQTAKVIVLFIVFIPLAFLIPSSSGFATTIFPLLAKSLVDSKTNQLQAYASSGSIMAFTFAIGLVNLITPTSGVVMGACSLSRMSYAKYLKAMLPIISYLFILCFILLLIGGALPDSIS"
ORIGIN
        1 ATGCACATTA AAGTGGAGAA TACCGAGATG AATAATTTTA ACTCGAACAT TAAGAAGAAG
       61 AAGCGCTTGA AGATGCTCTC TTCATTCAGT ATCTTGTTAT TGATCATGTT AGTTCTGATG
      121 TTGGTTAGTT GGATCTTATA CTGGAGTAAA ACCAAGACGG ACTTAGTCAA AACTATTTCC
      181 TTCAACGATT GGAAGTACGA TCCAATCCTT TCCCCAATCT ATAATGCATG GACTTCCAAG
      241 TACCCGAATA TCTCAGCGGG CAATTCGCAG ACGTGGATCG ATTTTATGAA CTCGAACTCC
      301 TCCCTGGGCT GGGTTTACAA CAGCCACGGA TGGATCAAGG ACTCATACAC CATCCAACAC
      361 AGTGGGGATG CCATCTTTAA TGGACTTGCC CCTATCCAGC CGATCGGTAT CATTGATGTA
      421 ATTTATGCAC CAATTAAGGG CTTTGTATTG AAAAGTAACA TTATTATTTT CACAATTAGC
      481 ATCGGAGCGT TCTTATATAT TTTGGTGTCC ACAAAAGCGC TGGAGGGTCT GAGTCAGGCT
      541 ATTATCGCTA AACTTAAGGG TAAAGAGGCA TTCGCGATCA TTCCGTTGAT GCTGTTTTTT
      601 AGTATCTTCG GGACTGTTGA AGGCTTCGCT GAAGAAACTT TAGGGTTTTA CATGATCTTT
      661 ATCCCCATCA TGTTAATGGC GGGGTTCGAT GTTTTCACGG GCGTTTTAAT CCTTATGGTC
      721 GGAGCGGGAA CAGGTGTTAT TGGATCAACC GTAAACCCTT TTACCATCCC CATTGCAGTA
      781 AGCGCAATTA ACTCAGGCAT CGACGCGTCA ACGGCAAAGC TGACTATCGG TGACGGCCTT
      841 GTTTGGCGCA TCATTTGCTG GTTAATTTTG ACTTCATTTA GCACGACATT CACCTTGTTA
      901 TACGCATTAA AGGTAAAAAA GAACCCTAGC AAATCCGTGA CTTTTTCAAC CTTAGAGGGT
      961 GACAAGGAGT TTTTCCTTGC CCATGTCTCT AAAACCATTA AGCTGGACTG GAAGAAAAAA
     1021 GTTAGCCTGG TAGCCTTCGC CATCTCTTTC CTTGTTATGA TTTTCTATCT GGTAGGCTGG
     1081 GACTCCATCT TCAATAACAC GAAAATGGCA GATCAAGCCA TCTGGATCAA AAAGAACATC
     1141 CCTTACCTTA CTGCTTTGAT CCCGGGCTGG GGAAACGGTG ATTTAGACAA CGTCGCTGCC
     1201 TTTTTCCTGC TGGCTTCGAT TACTTTAGCG ATCATTAATA GCATCGGCGA AGCGACTTTT
     1261 ATTAAAAAAT GGTTTGAGGG CGCCTCGGAT ATTCTTTCTG TCGCATTCAT TATCGCTACT
     1321 GCAGCCGGGG TGGGATATAT TTTAGTTCAG ACTAACCTTC AAAGCCTGTT CGTAAAGGGC
     1381 ATTCTTTCGT CCATCGGAGG TATTAATAAC CAAACCGCCA AGGTAATCGT TCTGTTCATC
     1441 GTTTTCATCC CGCTTGCGTT TCTGATCCCT TCCTCATCCG GGTTTGCAAC CACCATTTTC
     1501 CCCTTACTTG CCAAATCACT TGTAGACAGC AAGACGAACC AGCTTCAGGC TTATGCGTCT
     1561 AGTGGCAGTA TTATGGCCTT TACTTTTGCG ATCGGTCTGG TAAATCTGAT CACGCCCACT
     1621 TCGGGCGTTG TTATGGGTGC CTGTTCCCTG TCTCGCATGT CATATGCAAA GTACTTAAAG
     1681 GCCATGTTAC CAATTATTTC ATATCTGTTC ATTTTGTGCT TCATTTTACT TTTAATTGGG
     1741 GGCGCCTTGC CTGATTCTAT TTCCTGAAGA GCTTAGAGAC CGGTACTAGT TCCTGCAGGG
     1801 CTCGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1861 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1921 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1981 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     2041 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     2101 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     2161 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     2221 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     2281 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     2341 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     2401 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     2461 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2521 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
     2581 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
     2641 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
     2701 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
     2761 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
     2821 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG
     2881 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
     2941 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
     3001 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT
     3061 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
     3121 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
     3181 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
     3241 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
     3301 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC
     3361 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
     3421 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
     3481 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
     3541 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
     3601 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
     3661 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA
     3721 CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGAATTC
     3781 GCGGCCGCTT CTAGAGGCTC TTCGTAATTA CGAAGAGGTC TCTA
//