LOCUS       Exported     2753 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2753
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      766..813
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2641..2657
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2658..2689
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0874"
                     /note="RsmG: Ribosomal RNA small subunit methyltransferase"
                     /translation="MFNNWDIFLNYKNFTINQEIKNKLNLYYQILIQENQKYNLTRITQLNEVFEKHFLDSLLFVEQFQIIDQKIADIGTGAGFPGVVLKIFFPNIKLTLIESNNKKANFLKYLVQKLELNDVEILNKRVEELNEYKEQFDIVISRAVAYLDIILELGVQLVKVDGMFILLKGPKAHQEIKDLKNKDQKMNLKLVNIQELEDTGFGTRVNLFYKKIDHTNNLYPRKYQQILKESK"
     primer_bind     complement(818..834)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(901..1489)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1660..2520)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2521..2625)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /translation="MFNNWDIFLNYKNFTINQEIKNKLNLYYQILIQENQKYNLTRITQLNEVFEKHFLDSLLFVEQFQIIDQKIADIGTGAGFPGVVLKIFFPNIKLTLIESNNKKANFLKYLVQKLELNDVEILNKRVEELNEYKEQFDIVISRAVAYLDIILELGVQLVKVDGMFILLKGPKAHQEIKDLKNKDQKMNLKLVNIQELEDTGFGTRVNLFYKKIDHTNNLYPRKYQQILKESK"
                     /label="MMSYN1_0874"
                     /note="RsmG: Ribosomal RNA small subunit methyltransferase"
ORIGIN
        1 ATGTTTAACA ACTGGGACAT CTTCTTGAAT TACAAGAATT TCACGATAAA CCAGGAAATT
       61 AAAAATAAGT TGAATCTGTA TTATCAAATC CTGATTCAAG AAAATCAAAA GTATAACCTT
      121 ACCCGTATAA CCCAATTGAA CGAGGTATTC GAGAAGCATT TCTTGGATAG TCTGCTTTTT
      181 GTCGAACAGT TTCAGATTAT TGATCAGAAA ATTGCGGATA TCGGTACCGG CGCGGGTTTT
      241 CCTGGAGTCG TCCTTAAAAT ATTTTTCCCC AATATCAAGC TTACCCTTAT AGAGAGCAAT
      301 AACAAGAAAG CCAACTTTCT TAAATATCTT GTTCAAAAAC TGGAGCTGAA CGATGTCGAG
      361 ATTCTGAATA AGAGAGTAGA GGAGTTAAAT GAATACAAGG AGCAATTCGA TATTGTTATT
      421 AGTAGAGCGG TTGCGTATTT AGATATCATT TTGGAATTGG GTGTGCAACT GGTCAAGGTA
      481 GACGGGATGT TCATTTTATT AAAAGGCCCG AAGGCGCATC AAGAAATTAA AGATTTGAAG
      541 AATAAGGATC AGAAGATGAA CTTAAAATTA GTCAACATCC AGGAGCTTGA GGACACGGGT
      601 TTCGGTACCC GCGTGAATCT GTTTTACAAG AAAATAGACC ACACAAACAA CCTTTATCCT
      661 CGGAAGTACC AGCAAATACT GAAAGAGAGT AAGTGAAGAG CTTAGAGACC GGTACTAGTT
      721 CCTGCAGGGC TCGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
      781 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
      841 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
      901 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
      961 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1021 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1081 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1141 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1201 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1261 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1321 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     1381 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     1441 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     1501 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     1561 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     1621 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     1681 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     1741 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     1801 AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     1861 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     1921 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     1981 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     2041 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     2101 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     2161 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     2221 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     2281 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     2341 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     2401 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     2461 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     2521 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     2581 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA
     2641 GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG
     2701 AGTGAATTCG CGGCCGCTTC TAGAGGCTCT TCGTAATTAC GAAGAGGTCT CTA
//