LOCUS       Exported     2654 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2654
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      667..714
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2542..2558
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2559..2590
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /translation="MKQKTINLPNILTTIRILLVPIIIVLLLVDHYMNGLVFTNFSKFAWFISGAIFIIASLTDFLDGWIARKYNLVTNFGKFFDPIADKLLVNATLILFSSLGVLPIWMTVVLILRDTFIDFIRMILSSKGITLAAGMGGKLKTTFQMIGLSLLFFINLKIFGWSEWDWQNQLVLIPMYIATFFSIYSGVIYFLKARSNLF"
                     /label="MMSYN1_0875"
                     /note="PgsA: CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase"
     primer_bind     complement(719..735)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(802..1390)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1561..2421)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2422..2526)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     CDS             complement(1..0)
                     /note="PgsA: CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase"
                     /translation="MKQKTINLPNILTTIRILLVPIIIVLLLVDHYMNGLVFTNFSKFAWFISGAIFIIASLTDFLDGWIARKYNLVTNFGKFFDPIADKLLVNATLILFSSLGVLPIWMTVVLILRDTFIDFIRMILSSKGITLAAGMGGKLKTTFQMIGLSLLFFINLKIFGWSEWDWQNQLVLIPMYIATFFSIYSGVIYFLKARSNLF"
                     /label="MMSYN1_0875"
ORIGIN
        1 ATGAAACAAA AGACTATCAA CCTTCCCAAT ATCTTGACTA CGATCCGTAT TCTGCTTGTG
       61 CCTATCATTA TCGTCCTTCT GCTGGTGGAC CACTATATGA ACGGATTGGT GTTCACTAAT
      121 TTCTCTAAGT TCGCGTGGTT CATTTCCGGG GCGATCTTTA TTATTGCGTC TTTGACAGAC
      181 TTCCTGGACG GCTGGATTGC CCGTAAGTAT AACCTGGTCA CAAACTTCGG GAAGTTCTTC
      241 GACCCCATTG CCGACAAACT TTTAGTAAAT GCAACACTGA TCTTATTCAG CTCCCTGGGA
      301 GTCTTACCTA TCTGGATGAC CGTTGTATTA ATTTTACGTG ATACTTTCAT TGATTTTATC
      361 CGCATGATCT TATCGAGCAA AGGAATCACT TTGGCTGCAG GCATGGGAGG AAAATTAAAG
      421 ACGACGTTTC AGATGATTGG CCTTTCGTTG TTGTTCTTCA TTAACTTGAA GATTTTCGGA
      481 TGGTCGGAGT GGGACTGGCA AAATCAACTT GTGCTTATCC CGATGTATAT CGCCACATTC
      541 TTCAGCATTT ACTCCGGGGT GATTTACTTC CTGAAAGCAC GTTCGAATTT GTTTTGAAGA
      601 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG
      661 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
      721 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
      781 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
      841 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
      901 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
      961 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1021 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1081 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1141 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1201 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1261 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1321 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1381 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1441 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1501 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     1561 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     1621 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     1681 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     1741 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     1801 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     1861 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     1921 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     1981 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     2041 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     2101 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     2161 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     2221 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     2281 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     2341 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2401 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2461 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     2521 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     2581 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA
     2641 CGAAGAGGTC TCTA
//