LOCUS       Exported     3638 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3638
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1651..1698
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3526..3542
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3543..3574
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0876"
                     /note="amino acid permease"
                     /translation="MKNKGKLLEFLTLFAMTIGSVVGAGVYFKNKEILFDTRNPIIAIILWIIVGSVCVSMVYLFLEIASSTKNGGSGTIGVWTKLFINRKVGSFFAILNAFFYLPVMQSMFISFFITFILMMFSTVQLKGIHFLLIFLTTGIAIIIINALINVFDLSISRKYQAFGTIFKFIPLAIALIAGVVLFDQNGAFLSGGINITNPTGGTSKVEWSTNNFNPLLFFRGFGGILFAFDGFIFICNSQRKAKYKDVVPKALIFGMIFVSVFYTLIAVSLLMGSPDGSIGALLEKLFNGGKVLSSSDSSTLSRVANILTSVIIIIICSIGANNLSYVSFVVIESDVIDKLYLTSQKNISAKRIAIIQVSVATAIYSTFILVGTLATVGLTNTATVEQAVSSTNGLIYPIQIIATSNACLSFIMIITLIIGALFNRKTNKVEVEKKKGFVVLGSIAACCLVLFVTMSLFTILVPLDVINKNNNNSNWFTSNYYQGPLFILLTLLELGSVFIFWCIQEKRRKKYDLENPEIQIIAKPTV"
     primer_bind     complement(1703..1719)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1786..2374)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2545..3405)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3406..3510)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0876"
                     /note="amino acid permease"
                     /translation="MKNKGKLLEFLTLFAMTIGSVVGAGVYFKNKEILFDTRNPIIAIILWIIVGSVCVSMVYLFLEIASSTKNGGSGTIGVWTKLFINRKVGSFFAILNAFFYLPVMQSMFISFFITFILMMFSTVQLKGIHFLLIFLTTGIAIIIINALINVFDLSISRKYQAFGTIFKFIPLAIALIAGVVLFDQNGAFLSGGINITNPTGGTSKVEWSTNNFNPLLFFRGFGGILFAFDGFIFICNSQRKAKYKDVVPKALIFGMIFVSVFYTLIAVSLLMGSPDGSIGALLEKLFNGGKVLSSSDSSTLSRVANILTSVIIIIICSIGANNLSYVSFVVIESDVIDKLYLTSQKNISAKRIAIIQVSVATAIYSTFILVGTLATVGLTNTATVEQAVSSTNGLIYPIQIIATSNACLSFIMIITLIIGALFNRKTNKVEVEKKKGFVVLGSIAACCLVLFVTMSLFTILVPLDVINKNNNNSNWFTSNYYQGPLFILLTLLELGSVFIFWCIQEKRRKKYDLENPEIQIIAKPTV"
ORIGIN
        1 ATGAAAAATA AGGGCAAGCT TCTGGAGTTC TTGACACTGT TCGCCATGAC GATTGGTTCT
       61 GTGGTTGGAG CGGGGGTATA TTTCAAGAAC AAAGAGATTT TATTCGATAC CCGCAATCCC
      121 ATAATCGCGA TCATTTTGTG GATCATTGTA GGATCCGTAT GTGTCTCGAT GGTCTATTTA
      181 TTTCTTGAAA TCGCATCTAG TACTAAGAAC GGAGGTTCTG GAACAATTGG TGTATGGACC
      241 AAATTGTTCA TTAATCGCAA AGTAGGATCC TTTTTCGCGA TTTTAAATGC ATTCTTTTAT
      301 CTTCCCGTGA TGCAGTCAAT GTTTATCTCA TTCTTCATCA CATTCATCTT AATGATGTTT
      361 AGTACAGTAC AACTTAAAGG CATCCATTTT TTACTTATCT TTTTAACTAC TGGAATTGCG
      421 ATTATCATTA TTAATGCTCT TATTAACGTT TTTGACTTGT CTATCAGCCG TAAATATCAA
      481 GCGTTCGGGA CTATTTTCAA GTTTATCCCT TTAGCAATCG CACTTATCGC AGGAGTGGTA
      541 CTGTTTGATC AAAATGGTGC CTTTTTGAGC GGAGGTATCA ACATTACTAA TCCAACCGGC
      601 GGTACATCTA AGGTAGAGTG GTCCACAAAC AACTTTAACC CGCTTCTGTT TTTTCGCGGA
      661 TTTGGTGGAA TTTTGTTCGC TTTCGACGGC TTCATTTTCA TCTGTAACTC ACAGCGTAAG
      721 GCCAAGTATA AAGACGTTGT TCCAAAAGCT TTAATCTTTG GAATGATCTT TGTTTCGGTG
      781 TTCTACACCT TGATTGCAGT CTCGTTATTA ATGGGTTCAC CTGATGGGAG CATTGGAGCG
      841 TTATTAGAAA AGCTTTTCAA TGGGGGCAAG GTTCTGTCCT CTTCTGACTC GAGTACATTA
      901 TCTCGCGTTG CTAACATCTT AACCTCAGTA ATCATCATCA TTATCTGTTC AATCGGGGCC
      961 AATAATCTGT CATATGTCTC ATTCGTCGTC ATCGAGTCGG ATGTTATTGA CAAATTGTAC
     1021 TTGACTAGTC AGAAAAACAT CTCTGCGAAG CGGATCGCTA TCATCCAGGT GTCCGTAGCC
     1081 ACGGCAATCT ACTCAACCTT CATTTTGGTT GGGACCCTTG CTACAGTGGG CCTGACGAAT
     1141 ACCGCAACTG TCGAACAAGC TGTATCGTCA ACCAACGGTC TGATTTATCC GATCCAAATT
     1201 ATCGCGACCT CTAACGCGTG TCTGTCTTTC ATCATGATTA TTACGCTTAT TATCGGTGCA
     1261 TTATTCAACC GCAAAACTAA TAAGGTTGAG GTAGAGAAGA AAAAGGGGTT TGTAGTTCTG
     1321 GGGTCTATTG CCGCATGTTG TTTAGTATTG TTTGTGACGA TGAGTCTGTT TACCATTTTG
     1381 GTTCCGTTGG ACGTAATTAA TAAAAACAAC AACAACTCAA ACTGGTTCAC ATCTAATTAC
     1441 TATCAGGGGC CATTGTTCAT TTTGCTTACT TTGTTAGAGC TTGGGTCCGT GTTTATTTTT
     1501 TGGTGTATCC AAGAAAAGCG TCGTAAAAAG TATGATTTAG AGAACCCGGA AATCCAGATT
     1561 ATTGCGAAAC CAACTGTGTG AAGAGCTTAG AGACCGGTAC TAGTTCCTGC AGGGCTCGAG
     1621 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1681 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1741 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1801 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1861 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1921 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1981 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     2041 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     2101 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     2161 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     2221 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     2281 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2341 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     2401 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     2461 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     2521 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     2581 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     2641 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC
     2701 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     2761 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     2821 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     2881 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     2941 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     3001 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     3061 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     3121 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     3181 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     3241 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     3301 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     3361 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     3421 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     3481 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA
     3541 GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGA ATTCGCGGCC
     3601 GCTTCTAGAG GCTCTTCGTA ATTACGAAGA GGTCTCTA
//