LOCUS       Exported     2738 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2738
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      751..798
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2626..2642
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2643..2674
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0877"
                     /note="membrane protein, putative"
                     /translation="MITYKEKKDNNLELQKDKKIKRVQSLRQYFLLSTNKIALLATLLALQILLTLFSKYVMGALVIFPSAPYLKLEINYWVSTVVLTATNLFWSLIFTVASVWMRLLLGSEPIGLLSLMLVDSSAIIGFATVFYIVKKMFIESNKSEAFAKFEILFVIFASVIATLFGGLVAYISNATFIFDLYSIPRPFGPILAVTFMFTIIKLVVNHAIFCIIYKRVKVLIRKIIRS"
     primer_bind     complement(803..819)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(886..1474)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1645..2505)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2506..2610)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0877"
                     /note="membrane protein, putative"
                     /translation="MITYKEKKDNNLELQKDKKIKRVQSLRQYFLLSTNKIALLATLLALQILLTLFSKYVMGALVIFPSAPYLKLEINYWVSTVVLTATNLFWSLIFTVASVWMRLLLGSEPIGLLSLMLVDSSAIIGFATVFYIVKKMFIESNKSEAFAKFEILFVIFASVIATLFGGLVAYISNATFIFDLYSIPRPFGPILAVTFMFTIIKLVVNHAIFCIIYKRVKVLIRKIIRS"
ORIGIN
        1 ATGATTACTT ACAAAGAGAA AAAAGACAAC AACCTGGAAC TTCAGAAAGA CAAAAAGATC
       61 AAACGCGTGC AGTCCCTGCG CCAATACTTT TTGCTTAGCA CTAACAAAAT CGCTCTTTTG
      121 GCTACATTAC TTGCCTTGCA GATTTTACTG ACATTATTCA GCAAATACGT GATGGGAGCG
      181 TTGGTCATCT TCCCCTCGGC GCCATACCTT AAGTTGGAAA TCAATTACTG GGTGTCTACG
      241 GTAGTCTTAA CTGCAACCAA CCTTTTTTGG TCACTGATTT TCACCGTGGC CTCTGTGTGG
      301 ATGCGTTTAT TGTTAGGCTC GGAGCCTATC GGACTGTTGT CGTTAATGTT AGTAGATAGT
      361 TCCGCGATTA TTGGTTTCGC CACGGTCTTT TATATTGTAA AGAAGATGTT TATCGAATCA
      421 AATAAGAGTG AGGCATTTGC CAAGTTTGAA ATCTTATTCG TTATTTTTGC CTCCGTGATC
      481 GCAACACTGT TTGGTGGTCT GGTGGCCTAT ATCTCTAATG CTACATTTAT CTTTGACCTG
      541 TATTCAATTC CTCGTCCCTT CGGACCTATC CTTGCCGTCA CTTTCATGTT TACGATTATT
      601 AAATTAGTCG TGAATCATGC TATCTTCTGT ATCATCTATA AGCGCGTTAA GGTATTGATT
      661 CGTAAGATCA TCCGCTCCTG AAGAGCTTAG AGACCGGTAC TAGTTCCTGC AGGGCTCGAG
      721 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
      781 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
      841 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
      901 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
      961 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1021 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1081 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1141 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1201 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1261 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1321 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1381 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1441 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     1501 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     1561 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     1621 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     1681 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     1741 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC
     1801 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     1861 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     1921 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     1981 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     2041 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     2101 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     2161 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     2221 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     2281 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     2341 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     2401 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     2461 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     2521 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     2581 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA
     2641 GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGA ATTCGCGGCC
     2701 GCTTCTAGAG GCTCTTCGTA ATTACGAAGA GGTCTCTA
//