LOCUS       Exported     4715 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4715
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2728..2775
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4603..4619
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4620..4651
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             1..0
                     /label="MMSYN1_0879"
                     /note="putative magnesium-importing ATPase"
                     /translation="MFKTKKGNLKSLDYKKQDYVIKLSNTNSNNLESILDSKIGLNNQTRQNNISKFGSNQIVVKKFLIFKKILETLIEPFNLLLLFIGILELIIYFLFQRNWITLISAFIIFFMIFLASIVDFIQEYKAYKFNLKLTKIIENDVFVVNDQIKDFNNLNYQNIKNNLIKEKQSNLTIGDVVYLSKGDIIPSDCRIIWSEDLYLDESTLTGESKAIKKQTTNTKTNFLELENILFKETLIVSGNCLAVVININKDNYSNSLLDLIDDEVITDYEKGINKVTKILIYLISILVFIITFISLLKTGISNWTSSLVFGLSIAVSLTPEALPAIISSNLKLASKRLSKNKVVIKKLSVLQNIGSVNILATDKTGTLTLDTTNIETYLDINNQKNKLLMQYFFYNAYFQNNLFDTIDKAIIDQFKTNISDIKLIDHLSFDHNFRISSVLINFNSSNLLITKGSLEEILEITSFINVNNQVINLCDNYKNMIIDQVNSYTKKGYKVLVLSYKNSDVIDNKNLIYLGMVVFSDQIRENVKQVIDTFKAYDIDIKVLSGDNLYTCKNVCDQVGINSNTSLIGKQINNLTKEELIKISQSVNIFYKLSPLDKAKIIDSLKSNNVVGFLGDGVNDAVALKKADVGISVNNASSLAKQSADVILLEKDLNALEHAFIIGRKTFSNAIKYIKITVASNFGILLTLLLATSLFKFEVMSPIQLLIQNLIFDFANLVFVFDNVDESSIKKPQKWNIKSIIPFAIFNGLTQVIISFINFMILYFGFNIKGLDTYSIELFQTCYFIECILTHIMIILVLRTDKLSFFKSIASKQMLISMLFFSVVCFMIVFISSSFNSLGFKMMIGNFNNINLSWWFLILFGLEILSWIISELIKKIYLIIFKNWI"
     primer_bind     complement(2780..2796)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2863..3451)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3622..4482)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(4483..4587)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0879"
                     /note="putative magnesium-importing ATPase"
                     /translation="MFKTKKGNLKSLDYKKQDYVIKLSNTNSNNLESILDSKIGLNNQTRQNNISKFGSNQIVVKKFLIFKKILETLIEPFNLLLLFIGILELIIYFLFQRNWITLISAFIIFFMIFLASIVDFIQEYKAYKFNLKLTKIIENDVFVVNDQIKDFNNLNYQNIKNNLIKEKQSNLTIGDVVYLSKGDIIPSDCRIIWSEDLYLDESTLTGESKAIKKQTTNTKTNFLELENILFKETLIVSGNCLAVVININKDNYSNSLLDLIDDEVITDYEKGINKVTKILIYLISILVFIITFISLLKTGISNWTSSLVFGLSIAVSLTPEALPAIISSNLKLASKRLSKNKVVIKKLSVLQNIGSVNILATDKTGTLTLDTTNIETYLDINNQKNKLLMQYFFYNAYFQNNLFDTIDKAIIDQFKTNISDIKLIDHLSFDHNFRISSVLINFNSSNLLITKGSLEEILEITSFINVNNQVINLCDNYKNMIIDQVNSYTKKGYKVLVLSYKNSDVIDNKNLIYLGMVVFSDQIRENVKQVIDTFKAYDIDIKVLSGDNLYTCKNVCDQVGINSNTSLIGKQINNLTKEELIKISQSVNIFYKLSPLDKAKIIDSLKSNNVVGFLGDGVNDAVALKKADVGISVNNASSLAKQSADVILLEKDLNALEHAFIIGRKTFSNAIKYIKITVASNFGILLTLLLATSLFKFEVMSPIQLLIQNLIFDFANLVFVFDNVDESSIKKPQKWNIKSIIPFAIFNGLTQVIISFINFMILYFGFNIKGLDTYSIELFQTCYFIECILTHIMIILVLRTDKLSFFKSIASKQMLISMLFFSVVCFMIVFISSSFNSLGFKMMIGNFNNINLSWWFLILFGLEILSWIISELIKKIYLIIFKNWI"
ORIGIN
        1 ATGTTCAAAA CGAAGAAGGG CAACCTGAAA TCGTTAGACT ATAAGAAGCA GGATTACGTC
       61 ATTAAGTTAT CGAACACTAA CTCCAATAAC TTGGAATCCA TTCTGGACTC TAAAATCGGT
      121 CTTAATAATC AAACTCGTCA GAATAACATC TCGAAATTCG GGTCGAATCA GATCGTGGTG
      181 AAGAAATTTC TGATCTTTAA GAAAATCCTG GAAACCTTAA TTGAGCCTTT CAATTTGTTG
      241 TTGTTATTCA TTGGAATCTT AGAACTGATT ATCTACTTCT TGTTCCAACG TAATTGGATC
      301 ACCCTTATCA GCGCTTTTAT CATCTTCTTC ATGATCTTTT TGGCAAGCAT CGTTGATTTT
      361 ATCCAGGAAT ACAAAGCGTA TAAGTTTAAC TTGAAGCTGA CAAAGATCAT CGAGAACGAC
      421 GTATTCGTGG TGAACGATCA GATTAAGGAC TTTAACAACC TGAACTACCA AAACATCAAG
      481 AATAATTTGA TCAAAGAAAA ACAGAGCAAC TTAACAATTG GAGATGTAGT GTATCTGAGC
      541 AAGGGGGACA TCATCCCTTC AGATTGTCGT ATTATTTGGA GTGAGGATCT GTATCTTGAT
      601 GAGAGCACGC TTACCGGCGA ATCTAAGGCG ATCAAGAAAC AAACCACAAA TACCAAAACA
      661 AACTTTTTGG AACTTGAAAA CATCTTGTTT AAGGAAACCC TGATTGTCTC AGGCAACTGT
      721 CTGGCAGTGG TAATTAACAT TAATAAGGAT AACTATTCGA ACTCGTTGTT AGATCTTATT
      781 GACGACGAAG TCATTACGGA TTATGAGAAA GGTATCAATA AAGTCACCAA AATCCTGATT
      841 TATTTAATCA GTATTCTTGT ATTTATCATC ACCTTTATTT CGTTGTTAAA GACGGGAATC
      901 AGCAACTGGA CCAGTTCGTT AGTATTTGGC TTATCTATTG CCGTAAGTCT TACGCCAGAA
      961 GCATTACCGG CGATTATTTC TTCAAATCTG AAATTGGCGT CGAAACGCCT GTCTAAAAAT
     1021 AAGGTGGTCA TCAAAAAATT AAGTGTTCTG CAAAACATCG GTTCGGTCAA TATTCTTGCC
     1081 ACTGACAAAA CGGGGACATT GACACTGGAC ACGACAAACA TCGAAACGTA CTTGGACATC
     1141 AATAACCAGA AGAACAAACT TTTGATGCAA TACTTCTTTT ACAACGCATA CTTTCAAAAC
     1201 AATTTGTTCG ACACAATCGA TAAGGCAATC ATTGATCAGT TCAAGACGAA TATCTCCGAC
     1261 ATCAAGCTTA TTGATCATCT TTCTTTCGAC CATAACTTCC GTATTTCGAG TGTTCTTATC
     1321 AACTTCAACT CGAGTAATCT GCTGATCACA AAAGGTAGTC TTGAAGAGAT CCTGGAAATC
     1381 ACATCGTTTA TCAACGTAAA TAACCAAGTC ATCAACTTGT GCGACAATTA CAAGAATATG
     1441 ATTATCGACC AAGTAAACTC TTACACGAAA AAGGGATACA AAGTCTTGGT CTTAAGCTAC
     1501 AAGAATTCAG ATGTGATCGA CAACAAAAAC CTTATTTATC TGGGTATGGT TGTTTTCTCG
     1561 GATCAAATTC GCGAAAACGT TAAACAAGTG ATTGACACTT TCAAGGCATA TGACATCGAT
     1621 ATTAAAGTCC TTTCGGGTGA TAATCTTTAC ACATGCAAGA ATGTCTGTGA TCAGGTTGGG
     1681 ATTAACTCTA ACACCTCATT GATTGGTAAG CAAATCAACA ACCTTACAAA AGAAGAATTG
     1741 ATTAAGATCT CCCAATCTGT AAATATTTTT TATAAATTAA GCCCGCTGGA CAAAGCAAAG
     1801 ATCATCGATT CCCTGAAAAG CAACAATGTA GTTGGCTTCT TGGGCGACGG AGTAAACGAT
     1861 GCAGTAGCGT TAAAGAAAGC AGATGTTGGG ATTTCTGTCA ACAACGCCTC ATCTCTTGCA
     1921 AAGCAATCCG CCGACGTAAT TTTGTTAGAA AAAGACTTAA ACGCCTTGGA GCACGCGTTC
     1981 ATCATCGGAC GTAAGACTTT CTCTAATGCC ATTAAGTATA TCAAGATCAC CGTCGCTTCC
     2041 AATTTCGGCA TCTTATTAAC TTTACTTTTA GCAACTTCTC TGTTCAAATT TGAGGTCATG
     2101 TCACCAATTC AATTATTGAT CCAAAATTTA ATCTTCGACT TTGCTAATTT GGTCTTTGTT
     2161 TTTGATAATG TGGACGAAAG CTCCATCAAA AAGCCGCAAA AGTGGAACAT CAAAAGTATC
     2221 ATCCCCTTCG CAATCTTCAA TGGACTGACC CAAGTTATTA TTAGCTTTAT CAATTTTATG
     2281 ATCTTGTACT TTGGATTCAA CATTAAGGGG CTTGATACCT ACTCTATTGA GCTGTTCCAA
     2341 ACTTGTTACT TCATCGAATG TATTCTTACC CATATCATGA TCATTCTTGT ATTACGTACC
     2401 GACAAGCTTT CATTTTTTAA AAGTATCGCT TCTAAGCAGA TGTTAATCTC CATGTTATTT
     2461 TTCAGTGTGG TTTGTTTTAT GATTGTTTTC ATTAGCTCAT CGTTTAATTC TTTAGGATTT
     2521 AAGATGATGA TTGGTAATTT TAATAACATT AACTTGAGCT GGTGGTTCTT GATTCTTTTC
     2581 GGCCTTGAGA TTTTATCTTG GATTATCTCT GAATTAATCA AGAAAATTTA CCTGATCATC
     2641 TTCAAGAACT GGATTTGAAG AGCTTAGAGA CCGGTACTAG TTCCTGCAGG GCTCGAGGTG
     2701 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     2761 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     2821 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     2881 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     2941 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     3001 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     3061 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     3121 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     3181 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     3241 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     3301 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     3361 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     3421 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     3481 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     3541 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     3601 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     3661 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     3721 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC
     3781 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     3841 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     3901 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
     3961 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     4021 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     4081 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     4141 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     4201 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
     4261 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     4321 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     4381 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     4441 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     4501 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     4561 GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA
     4621 GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGAATT CGCGGCCGCT
     4681 TCTAGAGGCT CTTCGTAATT ACGAAGAGGT CTCTA
//