LOCUS       Exported     3913 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3913
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2010..3899
                     /label="MMSYN1_0885"
                     /note="NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34."
                     /translation="MKSNYDVIVVGGGHAGVEAALASARLNKKTALINLYEDKIATMPCNPSVGGPAKGIVVREIDALGGEMAKAADATALQTKLLNSSRGPGVWALRVQSDKEEYSKYMRNVIKNQKNLDLITRACTGLVYDDNKTVTGIYLDDQTILNAKAVIITTGTYLKSEILKGVDRYESGPNNEKTTKGISKSLIDLGIKLMRFKTGTPARVYRDSVDLTNAVLEPGTDMKLAFSFSTNTYTPIEKQQPCYLIHSTLETKKIIEDNLEKSAMYSGTVESIGPRYCPSFEDKVVRFKEKDTHQIFIEPETLNGDTWYVQGFSTSMPIEVQELMLKSLPGFENVRVKHWAYAIEYDCIDPMQLSPSLELKDVRNLFTAGQINGTSGYEEAAGQGLIAGINASRKIDGLDPIILRRDEAYIGVMIDDLVNKGVWEPYRLLTSRAEHRLLLRNDNAETRLKQYGREIGLISDQEWNQYLIYVNEIEQAIKELKEIRFTPKSQLAINLKNKNQADLSHGYSGYEIIKIPTVDINELIEFIPSLQKLKTNQLQSIVIEIRFEGYVKKERQLVDKLVKLERKKIPLDINYSKVDNLATEAKDKLEKIRPLNIGQASRITGVNPADIQMLLFYLKKQYPLENIDN"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAGTCAAA CTATGATGTT ATCGTAGTCG
     2041 GAGGGGGTCA TGCTGGCGTT GAGGCCGCAC TTGCCTCTGC TCGCCTGAAT AAAAAGACAG
     2101 CCTTAATCAA CCTTTATGAG GATAAGATTG CGACTATGCC ATGTAATCCG TCGGTAGGGG
     2161 GTCCAGCGAA AGGTATCGTG GTCCGTGAGA TCGATGCCCT GGGCGGTGAG ATGGCGAAGG
     2221 CAGCGGACGC GACGGCCTTA CAGACCAAGC TGTTAAACTC TAGTCGTGGC CCAGGGGTTT
     2281 GGGCTTTGCG CGTTCAATCG GATAAGGAAG AGTATTCGAA ATATATGCGT AATGTAATCA
     2341 AAAACCAGAA AAATTTAGAT TTGATTACGC GCGCCTGCAC TGGATTGGTT TACGACGACA
     2401 ACAAAACGGT AACTGGCATT TACTTAGATG ATCAGACTAT CCTGAACGCA AAAGCCGTGA
     2461 TTATCACTAC GGGTACTTAC TTAAAGTCTG AGATCCTGAA AGGGGTAGAC CGTTACGAGT
     2521 CGGGACCGAA CAACGAAAAG ACGACTAAGG GCATTTCAAA GTCGTTGATC GACTTGGGGA
     2581 TTAAGCTGAT GCGTTTTAAA ACGGGCACGC CTGCTCGTGT ATACCGTGAT AGTGTGGATC
     2641 TGACTAATGC GGTCCTTGAG CCAGGGACGG ATATGAAATT GGCCTTTTCA TTTTCAACCA
     2701 ATACCTATAC CCCGATTGAA AAGCAACAGC CGTGCTACCT GATCCACTCC ACACTGGAAA
     2761 CGAAGAAAAT CATTGAGGAC AACTTAGAGA AATCCGCAAT GTACAGCGGC ACTGTGGAAT
     2821 CTATTGGGCC ACGCTATTGT CCCTCTTTCG AGGACAAAGT AGTACGCTTC AAGGAAAAGG
     2881 ATACTCATCA GATCTTTATC GAGCCAGAGA CTCTGAATGG AGATACGTGG TACGTTCAAG
     2941 GATTCAGTAC TTCCATGCCA ATTGAAGTGC AAGAGTTGAT GCTTAAGTCC CTTCCAGGCT
     3001 TTGAAAACGT GCGCGTTAAG CACTGGGCCT ATGCAATTGA ATACGACTGC ATCGATCCGA
     3061 TGCAATTGTC ACCGTCCTTA GAACTTAAAG ATGTCCGCAA CCTTTTTACG GCGGGTCAGA
     3121 TTAATGGAAC TAGCGGCTAC GAGGAAGCGG CTGGTCAAGG ATTGATCGCA GGCATTAACG
     3181 CTTCACGTAA GATCGATGGT TTGGATCCAA TTATCCTGCG TCGTGATGAG GCTTATATTG
     3241 GTGTAATGAT CGATGATCTG GTCAATAAAG GGGTGTGGGA ACCTTACCGC CTGTTGACTA
     3301 GTCGTGCGGA GCACCGCCTG CTTTTGCGCA ATGACAATGC GGAAACCCGT TTAAAACAAT
     3361 ATGGGCGCGA AATCGGCCTG ATCTCGGATC AGGAGTGGAA TCAGTACCTT ATCTACGTGA
     3421 ATGAAATCGA GCAGGCGATT AAAGAATTAA AAGAGATCCG TTTTACACCC AAGAGCCAAC
     3481 TGGCCATTAA TCTTAAAAAC AAAAATCAGG CCGACTTGAG CCACGGCTAC TCAGGGTACG
     3541 AGATCATCAA GATCCCAACA GTTGATATCA ATGAGCTTAT TGAGTTCATC CCGTCTTTAC
     3601 AAAAGCTTAA GACCAATCAG CTTCAGTCAA TCGTTATTGA GATCCGTTTC GAGGGGTATG
     3661 TGAAAAAAGA GCGTCAGCTT GTTGATAAAC TGGTCAAGTT GGAGCGTAAG AAGATCCCGT
     3721 TAGACATTAA TTACTCGAAG GTGGATAACC TGGCGACGGA GGCGAAGGAC AAGCTTGAGA
     3781 AGATCCGTCC GCTGAACATC GGTCAAGCTT CTCGTATCAC TGGGGTTAAC CCTGCGGACA
     3841 TCCAAATGCT TCTTTTCTAT CTGAAGAAAC AGTACCCCCT TGAAAATATT GATAACTGAA
     3901 GAGCTTAGAG ACC
//