LOCUS Exported 3367 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3367 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2010..3353 /label="MMSYN1_0887" /note="" /translation="MKIVIIGGAASGMTVASRLKKADKKAQIIVIQKEKYVSLGACGLPYFVANPSLKPTDLLARTVEQFIEQDILVYSESVVKKIDAENQKVWYEKNNQLLELDYDKLVISTGAKPIVPPIKGIDLPNIFTLTRLEDATELKEKLKDKNIKKVAVIGSGFIGLECCEMLEHFNKEIVLIEKTSRLNQRVFDQEITDLLEQNLIKNNVEIIKENGLKSITQTKDKRLSLTLDQNEELEVDLIILAIGFRPATEFLKDTKLEMLGNGAIVVDKHGRTNLKNIWSCGDCATVYHKITNQITYTPLATVARKFAKVVADDILNVNSEFVGTLQTAILKVFESELVATGVNETLAKELGYDIKTIFIKDSDHPSYYPNPTPLALKLILNKKTNTLIGAQMYGSNLSVLRINFLISLIWNQIEINQELTQVDLPYSPPFSRVVDIIHIALEKLIKN" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAAAATTGT CATTATCGGC GGAGCGGCTT 2041 CAGGCATGAC TGTGGCGAGT CGCTTAAAGA AAGCTGATAA GAAAGCACAA ATTATCGTAA 2101 TTCAAAAAGA AAAGTATGTT AGTTTAGGCG CGTGTGGCCT GCCATACTTC GTTGCTAACC 2161 CTTCATTGAA GCCAACTGAT CTTCTGGCCC GCACAGTCGA ACAGTTTATC GAGCAAGATA 2221 TCCTGGTGTA TTCTGAGTCT GTGGTCAAAA AGATTGATGC AGAGAACCAG AAGGTCTGGT 2281 ACGAGAAAAA TAATCAGCTT CTTGAGCTGG ATTATGATAA GTTGGTGATT AGCACCGGCG 2341 CAAAGCCTAT CGTACCCCCA ATCAAGGGTA TTGATCTGCC CAACATTTTC ACCTTGACGC 2401 GTCTGGAGGA TGCGACTGAA CTTAAAGAAA AGTTAAAAGA TAAAAATATC AAGAAAGTAG 2461 CTGTGATTGG TAGCGGGTTC ATTGGTCTGG AGTGCTGCGA GATGTTGGAA CATTTCAATA 2521 AGGAGATCGT GTTAATCGAA AAGACTTCGC GCCTGAATCA ACGTGTTTTC GATCAGGAAA 2581 TCACTGATCT TCTTGAGCAA AATCTTATCA AGAATAATGT CGAGATCATT AAGGAGAACG 2641 GGTTAAAGAG CATCACTCAA ACGAAAGATA AGCGCTTGTC GCTGACGTTA GATCAGAATG 2701 AGGAACTTGA AGTAGATCTG ATCATCTTGG CCATCGGTTT TCGCCCTGCT ACGGAATTTC 2761 TTAAAGATAC TAAGTTAGAA ATGTTGGGAA ATGGGGCCAT TGTCGTCGAT AAGCATGGTC 2821 GCACAAACTT GAAGAATATC TGGTCCTGTG GTGATTGCGC GACAGTTTAC CATAAAATTA 2881 CCAACCAGAT TACATATACG CCTTTGGCAA CCGTGGCGCG TAAGTTTGCG AAGGTAGTAG 2941 CAGACGACAT TCTGAATGTA AACAGCGAAT TCGTGGGTAC ACTGCAAACT GCGATCTTAA 3001 AAGTTTTCGA AAGTGAACTT GTGGCGACAG GTGTAAATGA AACATTAGCA AAAGAATTAG 3061 GATATGATAT TAAGACCATC TTTATCAAGG ATTCCGATCA TCCTTCGTAC TATCCTAATC 3121 CAACTCCGTT AGCGCTGAAA CTTATTCTTA ACAAAAAGAC CAATACGCTG ATCGGAGCAC 3181 AAATGTACGG TTCAAACTTG AGCGTGTTGC GTATTAATTT TCTTATCTCT CTTATTTGGA 3241 ATCAAATTGA GATCAATCAG GAACTTACAC AGGTGGACCT TCCGTACAGT CCCCCCTTCT 3301 CACGCGTAGT TGATATTATC CATATTGCTT TGGAAAAACT TATCAAGAAC TGAAGAGCTT 3361 AGAGACC //