LOCUS       Exported     2774 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2774
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      787..834
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2662..2678
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2679..2710
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0906"
                     /note=""
                     /translation="MKIKITKGGTNVSYRVDNTFLQIKNYNNFNHQINYELLKNFDFVPKLISNNQKEIVWEYIDGVEPVIDLGNINLIANQIKQIHNSNLKFPDNNLKQRVEYYKTKMSELNTSVEVISKYASLIDDILDSMEFNTPLHNDLFPFNMIQTENKIYFVDWEYATMGDKHFELAYLIETSNMSNQCEKVFLDLYRNYDEHKLLLNKIFVNYIVILWIRTQTKAPHNTTFFEQKIINYVAKLNI"
     primer_bind     complement(839..855)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(922..1510)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1681..2541)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2542..2646)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0906"
                     /note=""
                     /translation="MKIKITKGGTNVSYRVDNTFLQIKNYNNFNHQINYELLKNFDFVPKLISNNQKEIVWEYIDGVEPVIDLGNINLIANQIKQIHNSNLKFPDNNLKQRVEYYKTKMSELNTSVEVISKYASLIDDILDSMEFNTPLHNDLFPFNMIQTENKIYFVDWEYATMGDKHFELAYLIETSNMSNQCEKVFLDLYRNYDEHKLLLNKIFVNYIVILWIRTQTKAPHNTTFFEQKIINYVAKLNI"
ORIGIN
        1 ATGAAGATCA AGATCACGAA AGGTGGTACT AATGTCTCTT ACCGCGTTGA CAACACTTTT
       61 TTGCAAATCA AAAACTATAA CAATTTCAAT CACCAAATCA ATTACGAACT GTTAAAGAAC
      121 TTTGATTTTG TCCCAAAACT GATTTCCAAC AACCAAAAGG AGATTGTGTG GGAGTACATT
      181 GATGGTGTAG AACCCGTAAT CGATCTGGGC AACATTAATT TGATCGCCAA TCAGATCAAG
      241 CAGATTCATA ACTCAAATCT GAAGTTCCCT GACAACAATT TAAAGCAACG CGTCGAGTAC
      301 TATAAAACAA AGATGTCGGA ACTTAATACT TCAGTGGAAG TAATTAGCAA ATATGCGAGC
      361 TTGATTGATG ACATTCTTGA TTCGATGGAA TTCAACACGC CCTTACATAA CGATTTGTTT
      421 CCATTTAATA TGATCCAAAC CGAGAACAAG ATTTACTTCG TCGATTGGGA GTATGCAACA
      481 ATGGGGGATA AGCACTTTGA GTTAGCATAT CTTATCGAGA CATCAAACAT GTCAAATCAG
      541 TGTGAAAAAG TGTTCCTGGA TTTGTACCGC AACTATGACG AGCATAAGCT GTTACTGAAT
      601 AAGATCTTTG TGAATTACAT TGTAATTTTG TGGATTCGCA CCCAAACGAA GGCTCCACAC
      661 AATACGACAT TTTTCGAGCA AAAGATTATC AACTACGTTG CCAAGCTGAA CATTTGAAGA
      721 GCTTAGAGAC CGGTACTAGT TCCTGCAGGG CTCGAGGTGT CAATCGTCGG AGCCGCTGAG
      781 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
      841 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
      901 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
      961 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1021 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1081 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1141 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1201 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1261 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1321 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1381 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1441 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1501 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA
     1561 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
     1621 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG
     1681 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
     1741 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC
     1801 CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA
     1861 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA
     1921 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA
     1981 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT
     2041 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC
     2101 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT
     2161 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC
     2221 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG
     2281 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT
     2341 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC
     2401 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG
     2461 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC
     2521 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG
     2581 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT
     2641 TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG
     2701 GCTTTTGACT TGGTCAGGTG GAGTGAATTC GCGGCCGCTT CTAGAGGCTC TTCGTAATTA
     2761 CGAAGAGGTC TCTA
//