LOCUS       Exported     3248 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3248
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1261..1308
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3136..3152
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3153..3184
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             1..0
                     /label="MMSYN1_0908"
                     /note=""
                     /translation="MYKQSDKVMSYLNASKNKKAPKTKKETIKLVIKWLKVFGFLFILVSMLWGCVQMYQAQYSVNQIVDMTGKSVYAPGVSFEIILSSLGEKGSKVHHFVYDKGNYFEYGYNAITSWKETFKLTQSPFYGFFVYPTAWVLAGMVRLFSGTLNPLLDKSSQLSYGISAIFAIFLTTLLIKGITLSFGWKSQINQEKMQDIQLKIADIQAKYKDKKDMQSKQKQQLEIQALYKKENMSQFSALAGSFAPLPFLFAIYAIVRSTRALKIAAVGPIALIEGPWQQITSGNYIYIIILAIYLPLQAVSMLLPTLLQMKKQKSITLTEAQKKSRKKQLIMQVVMMFVFIIIIVSVATGVCIYWIFSSVLQIIQTYAFYLYNEKKRKAGNQERQRRLRQMERMNLK"
     primer_bind     complement(1313..1329)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1396..1984)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2155..3015)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3016..3120)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     CDS             complement(1..0)
                     /label="MMSYN1_0908"
                     /note=""
                     /translation="MYKQSDKVMSYLNASKNKKAPKTKKETIKLVIKWLKVFGFLFILVSMLWGCVQMYQAQYSVNQIVDMTGKSVYAPGVSFEIILSSLGEKGSKVHHFVYDKGNYFEYGYNAITSWKETFKLTQSPFYGFFVYPTAWVLAGMVRLFSGTLNPLLDKSSQLSYGISAIFAIFLTTLLIKGITLSFGWKSQINQEKMQDIQLKIADIQAKYKDKKDMQSKQKQQLEIQALYKKENMSQFSALAGSFAPLPFLFAIYAIVRSTRALKIAAVGPIALIEGPWQQITSGNYIYIIILAIYLPLQAVSMLLPTLLQMKKQKSITLTEAQKKSRKKQLIMQVVMMFVFIIIIVSVATGVCIYWIFSSVLQIIQTYAFYLYNEKKRKAGNQERQRRLRQMERMNLK"
ORIGIN
        1 ATGTATAAAC AAAGCGACAA AGTTATGTCG TACTTAAATG CAAGTAAGAA TAAGAAGGCC
       61 CCTAAAACCA AGAAGGAAAC GATCAAGCTT GTAATCAAGT GGTTAAAAGT CTTTGGATTT
      121 TTGTTCATTC TTGTTTCGAT GCTGTGGGGG TGCGTCCAAA TGTATCAGGC CCAATATAGC
      181 GTTAATCAAA TTGTTGACAT GACAGGTAAA AGCGTTTATG CCCCCGGTGT GAGCTTTGAA
      241 ATTATTCTCT CTTCATTAGG GGAGAAAGGC AGCAAGGTTC ACCATTTTGT GTACGACAAA
      301 GGGAACTACT TCGAGTACGG TTATAACGCG ATTACCTCGT GGAAGGAAAC ATTTAAGCTT
      361 ACCCAATCAC CGTTTTATGG GTTTTTTGTA TATCCTACGG CGTGGGTGTT GGCTGGCATG
      421 GTACGCCTGT TTTCTGGGAC TCTTAATCCC CTGCTGGACA AGAGCAGCCA ATTGTCCTAT
      481 GGGATTAGCG CAATCTTTGC TATTTTCCTT ACTACCCTTC TGATTAAGGG AATTACGCTG
      541 AGTTTTGGGT GGAAAAGCCA GATCAATCAA GAAAAAATGC AAGACATCCA GTTGAAGATC
      601 GCGGATATTC AAGCCAAATA TAAGGACAAA AAAGACATGC AATCCAAGCA GAAGCAACAA
      661 TTGGAAATTC AGGCATTGTA TAAAAAAGAA AATATGTCAC AATTTTCGGC TCTGGCTGGA
      721 TCCTTTGCGC CACTGCCATT CCTGTTCGCG ATCTATGCTA TCGTGCGCAG CACACGCGCT
      781 CTTAAGATTG CCGCAGTCGG CCCTATCGCA CTTATTGAGG GCCCATGGCA ACAAATTACA
      841 AGTGGCAATT ATATTTATAT CATCATCCTG GCGATTTATC TGCCACTGCA GGCAGTGAGT
      901 ATGTTGTTGC CGACGTTGTT GCAAATGAAG AAACAGAAGT CGATTACACT GACCGAAGCG
      961 CAAAAGAAGA GTCGTAAGAA ACAATTGATT ATGCAAGTCG TCATGATGTT TGTATTCATC
     1021 ATCATTATTG TATCCGTAGC AACTGGAGTA TGCATCTACT GGATCTTCTC CTCCGTTCTT
     1081 CAGATCATTC AAACTTACGC TTTTTACTTA TATAACGAGA AAAAACGCAA AGCTGGAAAT
     1141 CAGGAGCGCC AACGCCGCTT GCGTCAAATG GAGCGTATGA ACTTGAAGTG AAGAGCTTAG
     1201 AGACCGGTAC TAGTTCCTGC AGGGCTCGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1261 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1321 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1381 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1441 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1501 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1561 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1621 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1681 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1741 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1801 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1861 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1921 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1981 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     2041 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     2101 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     2161 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     2221 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     2281 TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     2341 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     2401 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     2461 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     2521 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     2581 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     2641 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     2701 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     2761 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     2821 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     2881 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     2941 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     3001 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     3061 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     3121 CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT
     3181 GACTTGGTCA GGTGGAGTGA ATTCGCGGCC GCTTCTAGAG GCTCTTCGTA ATTACGAAGA
     3241 GGTCTCTA
//