LOCUS BBF10K_000459 2649 bp DNA circular UNK 29-NOV-2020 DEFINITION Pichia ENO 5'-Homology Region in pOpen-v3. ACCESSION BBF10K_000459 VERSION BBF10K_000459.1 KEYWORDS BBFD_0017; Open Yeast Collection Part; OpenMTA; FreeGenes; Golden Gate Assembly. SOURCE synthetic DNA construct ORGANISM recombinant plasmid . REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission REFERENCE 2 (bases 2002 to 2649) AUTHORS Scott Pownall, Open Science Network TITLE Direct Submission JOURNAL https://biobricks.org/freegenes/ FEATURES Location/Qualifiers source 1..2001 /organism="recombinant plasmid" /mol_type="other DNA" /original_record="pOpen_v3.1" primer_bind complement(24..42) /label="T7 Reverse" /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7" /original_record="pOpen_v3.1" terminator 38..85 /label="T7 terminator" /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" /original_record="pOpen_v3.1" primer_bind complement(86..106) /label="M13 rev" /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd" /original_record="pOpen_v3.1" misc_feature 107..121 /label="AarI" /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" /original_record="pOpen_v3.1" misc_feature 122..172 /label="spacer" /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" /original_record="pOpen_v3.1" rep_origin complement(173..761) /direction=LEFT /label="ori" /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" /original_record="pOpen_v3.1" CDS complement(952..1812) /label="AmpR" /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW*" /original_record="pOpen_v3.1" misc_feature 1918..1932 /label="AarI" /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" /original_record="pOpen_v3.1" primer_bind 1933..1949 /label="M13 fwd" /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" /original_record="pOpen_v3.1" terminator 1950..1981 /label="tonB terminator" /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac" /original_record="pOpen_v3.1" protein_bind 2034..2041 /label="NotI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" /original_record="part" misc_feature 2042..2538 /label="Pichia ENO 5'-Homology Region" /original_record="part" primer_bind complement(2539..2558) /label="5' Barcode" /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" /original_record="part" misc_feature 2002..2021 /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" misc_feature complement(2570..2589) /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" misc_feature 2590..2649 /label="FreeGenes barcode" /ApEinfo_fwdcolor="#FF7E00" /ApEinfo_revcolor="#FF7E00" protein_bind join(2022..2027,2029..2032) /label="BsaI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" protein_bind complement(join(2559..2562,2564..2569)) /label="BsaI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" protein_bind 2611..2628 /label="I-SceI" /ApEinfo_fwdcolor="#4F0080" /ApEinfo_revcolor="#4F0080" ORIGIN 1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc 61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt 121 gatgacacac attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata 181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaaggccta ctattagcaa 781 caacgatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 1081 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc 1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 1921 ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac 1981 ttggttcagg tggagtggga gaagaattac tgacccctcg gggtctctaa aaagcggccg 2041 caagaccagc ttcgctctta attaagcggc ccaaagagtg agaggaaagt acctgggcaa 2101 aatcacacaa ttccaaacca tgctaaatga gatttaaaga gcaaacgatg gcaaaaggca 2161 accgttataa atgtgatctt tcttggcagc tatctgtcaa tttttctaag gaacagtgaa 2221 ttcatcatag gagagatgtt atacgttaca taatcataca tactgcatgt atctcaccta 2281 ctttacctca tcaactctaa aacagttcta gtcccaaccc cagattccta gtcatgacac 2341 aagtccgcac cggacaggac tcacaaccag caagagaagc taacaaattt acgccccggt 2401 aaaacattct ttaggggccg ttcaatggta attttcctct cacccgttta aacttacctc 2461 cgggcggtat cttcaataac ctctgttgtc cccgggtatc attggaaaca gtgagggacg 2521 ttgaacagaa gagaggatca tcacgggcaa ggattgtaaa ggtgagacca agacgatccg 2581 agccattacg gggctgagca ccgtgaaggt tagggataac agggtaatac acttttggtc 2641 ttatgattt //