LOCUS BBF10K_000462 2548 bp DNA circular UNK 05-NOV-2020 DEFINITION Pichia AOXTT 3'-Homology Region in pOpen-v3. ACCESSION BBF10K_000462 VERSION BBF10K_000462.1 KEYWORDS BBFD_0017; Open Yeast Collection Part; OpenMTA; FreeGenes; Golden Gate Assembly. SOURCE synthetic DNA construct ORGANISM recombinant plasmid . REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission REFERENCE 2 (bases 2002 to 2548) AUTHORS Scott Pownall, Open Science Network TITLE Direct Submission JOURNAL https://biobricks.org/freegenes/ FEATURES Location/Qualifiers source 1..2001 /organism="recombinant plasmid" /mol_type="other DNA" /original_record="pOpen_v3.1" primer_bind complement(24..42) /label="T7 Reverse" /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7" /original_record="pOpen_v3.1" terminator 38..85 /label="T7 terminator" /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" /original_record="pOpen_v3.1" primer_bind complement(86..106) /label="M13 rev" /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd" /original_record="pOpen_v3.1" misc_feature 107..121 /label="AarI" /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" /original_record="pOpen_v3.1" misc_feature 122..172 /label="spacer" /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" /original_record="pOpen_v3.1" rep_origin complement(173..761) /direction=LEFT /label="ori" /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" /original_record="pOpen_v3.1" CDS complement(952..1812) /label="AmpR" /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW*" /original_record="pOpen_v3.1" misc_feature 1918..1932 /label="AarI" /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" /original_record="pOpen_v3.1" primer_bind 1933..1949 /label="M13 fwd" /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" /original_record="pOpen_v3.1" terminator 1950..1981 /label="tonB terminator" /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac" /original_record="pOpen_v3.1" primer_bind 2033..2052 /label="3' Barcode" /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" /original_record="part" misc_feature 2053..2448 /label="Pichia AOXTT 3'-Homology Region 396bp" /original_record="part" protein_bind 2449..2456 /label="NotI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" /original_record="part" misc_feature 2002..2021 /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" misc_feature complement(2469..2488) /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" misc_feature 2489..2548 /label="FreeGenes barcode" /ApEinfo_fwdcolor="#FF7E00" /ApEinfo_revcolor="#FF7E00" protein_bind join(2022..2027,2029..2032) /label="BsaI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" protein_bind complement(join(2458..2461,2463..2468)) /label="BsaI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" protein_bind 2510..2527 /label="I-SceI" /ApEinfo_fwdcolor="#4F0080" /ApEinfo_revcolor="#4F0080" ORIGIN 1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc 61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt 121 gatgacacac attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata 181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaaggccta ctattagcaa 781 caacgatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 1081 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc 1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 1921 ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac 1981 ttggttcagg tggagtggga gaagaattac tgacccctcg gggtctctag actatcgtgt 2041 tgcatctgcg gctcgagaat tatggcttaa tcaagtgaat acatcaaagt caaacttaaa 2101 atacattctt cgcaggcttg gcttgccaca tagttttctt cacaatgctg caaatgacgc 2161 ttattatacc cttttggcat cgttgaagct tgcacaaacg aacgtcccac ttaatcttct 2221 gtactctgaa gaggagtggg aaataccaag aaaaacatca aactcgaatg attttcccga 2281 acccctacca caagatattc atcagctgcg agataggctg atcaggagca agctcgtacg 2341 agaagaaaca aaatgacaaa aaaaatccta tactatatag gttacaaata aaaaagtatc 2401 aaaaatgaag cctgcatctc tcaggcaaat ggcattctga catcctctgc ggccgcacga 2461 atgagaccaa ggatatgtag acaccgcccc cggtgtgcta ggatggctgt agggataaca 2521 gggtaatggc ttcgcgggat tttaactt //