LOCUS BBF10K_000463 3152 bp DNA circular UNK 27-MAY-2018 DEFINITION Sc2micron in pOpen-v3. ACCESSION BBF10K_000463 VERSION BBF10K_000463.1 KEYWORDS BBFD_0017; Open Yeast Collection Part; OpenMTA; FreeGenes; Golden Gate Assembly. SOURCE synthetic DNA construct ORGANISM recombinant plasmid . REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission REFERENCE 2 (bases 2002 to 3152) AUTHORS Scott Pownall, Open Science Network TITLE Direct Submission JOURNAL https://biobricks.org/freegenes/ FEATURES Location/Qualifiers CDS complement(483..1343) /label="AmpR" /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW*" /original_record="pOpen_v3.1" misc_feature 1449..1463 /label="AarI" /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" /original_record="pOpen_v3.1" primer_bind 1464..1480 /label="M13 fwd" /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc" /original_record="pOpen_v3.1" terminator 1481..1512 /label="tonB terminator" /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac" /original_record="pOpen_v3.1" primer_bind complement(2707..2725) /label="T7 Reverse" /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7" /original_record="pOpen_v3.1" terminator 2721..2768 /label="T7 terminator" /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295" /original_record="pOpen_v3.1" primer_bind complement(2769..2789) /label="M13 rev" /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd" /original_record="pOpen_v3.1" misc_feature 2790..2804 /label="AarI" /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" /original_record="pOpen_v3.1" misc_feature 2805..2855 /label="spacer" /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67" /original_record="pOpen_v3.1" primer_bind 1541..1560 /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" /original_record="part" protein_bind join(1561..1566,1568..1571) /label="BsaI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" /original_record="part" protein_bind 1572..1577 /label="AvrII" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" /original_record="part" origin 1578..2578 /label="2micron origin" /original_record="part" misc_feature 1578..1641 /label="repeat-1" /ApEinfo_fwdcolor="#800080" /ApEinfo_revcolor="#800080" /original_record="part" misc_feature 1642..1701 /label="repeat-2" /ApEinfo_fwdcolor="#B284BE" /ApEinfo_revcolor="#B284BE" /original_record="part" misc_feature 1702..1766 /label="repeat-3" /ApEinfo_fwdcolor="#800080" /ApEinfo_revcolor="#800080" /original_record="part" misc_feature 1767..1826 /label="repeat-4" /ApEinfo_fwdcolor="#B284BE" /ApEinfo_revcolor="#B284BE" /original_record="part" misc_feature 1827..1890 /label="repeat-5" /ApEinfo_fwdcolor="#800080" /ApEinfo_revcolor="#800080" /original_record="part" protein_bind 2579..2584 /label="AgeI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" /original_record="part" protein_bind complement(join(2585..2588,2590..2595)) /label="BsaI" /ApEinfo_fwdcolor="#00FF2C" /ApEinfo_revcolor="#00FF2C" /original_record="part" primer_bind complement(2596..2616) /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" /original_record="part" misc_feature 2617..2675 /label="FreeGenes barcode" /ApEinfo_fwdcolor="#FF7E00" /ApEinfo_revcolor="#FF7E00" /original_record="part" protein_bind 2637..2654 /label="I-SceI" /original_record="part" misc_feature 2002..2021 /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" misc_feature complement(3073..3092) /label="FreeGenes dialout primer" /ApEinfo_fwdcolor="#0048BA" /ApEinfo_revcolor="#0048BA" misc_feature 3093..3152 /label="FreeGenes barcode" /ApEinfo_fwdcolor="#FF7E00" /ApEinfo_revcolor="#FF7E00" ORIGIN 1 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 61 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 121 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaaggcct 301 actattagca acaacgatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 601 cccagtgctg caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata 661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 781 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 1021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 1081 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 1141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 1201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 1261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 1321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 1381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 1441 gttccgcgca cctgcaccag tcagtaaaac gacggccagt agtcaaaagc ctccgaccgg 1501 aggcttttga cttggttcag gtggagtggg agtagtcttc aagaattact gacccctcgg 1561 ggtctctaaa acctaggaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg 1621 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca 1681 acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg 1741 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa 1801 atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa 1861 aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct 1921 ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta 1981 gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc 2041 gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga 2101 ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat 2161 gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg 2221 tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta 2281 caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt 2341 tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga 2401 gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt 2461 agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtcat cagagcgctt 2521 ttggttttca aaagcgctct gaagttccta tactttctag ctagagaata ggaacttcac 2581 cggtaaggtg agaccaaggc ccagaaggat acaactagca ctgcacatta aaagactagg 2641 gataacaggg taattgtagc gtcgtctaat tctcagaaga ccacgctgag gtgtcaatcg 2701 tcggagccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg 2761 gttttttgca tggtcatagc tgtttcctga gagcttggca ggtgatgaca cacattaaca 2821 aatttcgtga ggagtctcca gaagaatgcc attaatttcc ataggctccg cccccctgac 2881 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 2941 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 3001 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 3061 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 3121 cccgttcagc ccgaccgctg cgccttatcc gg //