LOCUS       BBF10K_000467           4094 bp    DNA     circular UNK 09-JUN-2018
DEFINITION  Yeast marker cassette ADE2 in pOpen-v3.
ACCESSION   BBF10K_000467
VERSION     BBF10K_000467.1
KEYWORDS    BBFD_0017; Open Yeast Collection Part; OpenMTA; FreeGenes; Golden
            Gate Assembly.
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
            .
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
REFERENCE   2  (bases 2002 to 4094)
  AUTHORS   Scott Pownall, Open Science Network
  TITLE     Direct Submission
  JOURNAL   https://biobricks.org/freegenes/
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /original_record="pOpen_v3.1"
     primer_bind     complement(24..42)
                     /label="T7 Reverse"
                     /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7"
                     /original_record="pOpen_v3.1"
     terminator      38..85
                     /label="T7 terminator"
                     /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295"
                     /original_record="pOpen_v3.1"
     primer_bind     complement(86..106)
                     /label="M13 rev"
                     /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd"
                     /original_record="pOpen_v3.1"
     misc_feature    107..121
                     /label="AarI"
                     /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67"
                     /original_record="pOpen_v3.1"
     misc_feature    122..172
                     /label="spacer"
                     /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67"
                     /original_record="pOpen_v3.1"
     rep_origin      complement(173..761)
                     /direction=LEFT
                     /label="ori"
                     /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc"
                     /original_record="pOpen_v3.1"
     CDS             complement(952..1812)
                     /label="AmpR"
                     /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW*"
                     /original_record="pOpen_v3.1"
     misc_feature    1918..1932
                     /label="AarI"
                     /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295"
                     /original_record="pOpen_v3.1"
     primer_bind     1933..1949
                     /label="M13 fwd"
                     /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc"
                     /original_record="pOpen_v3.1"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac"
                     /original_record="pOpen_v3.1"
     source          2022..4014
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /original_record="part"
     promoter        2033..2209
                     /label="AgTEF Promoter"
                     /original_record="part"
     CDS             2210..3925
                     /label="ADE2 selection marker"
                     /note="Complements ADE2 auxotrophic mutations in yeast.
                     ADE2 mutants require adenine"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
                     /original_record="part"
     terminator      3926..4003
                     /label="AgTEF Terminator"
                     /original_record="part"
     misc_feature    2002..2021
                     /label="FreeGenes dialout primer"
                     /ApEinfo_fwdcolor="#0048BA"
                     /ApEinfo_revcolor="#0048BA"
     misc_feature    complement(4015..4034)
                     /label="FreeGenes dialout primer"
                     /ApEinfo_fwdcolor="#0048BA"
                     /ApEinfo_revcolor="#0048BA"
     misc_feature    4035..4094
                     /label="FreeGenes barcode"
                     /ApEinfo_fwdcolor="#FF7E00"
                     /ApEinfo_revcolor="#FF7E00"
     protein_bind    join(2022..2027,2029..2032)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    complement(join(4004..4007,4009..4014))
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    4056..4073
                     /label="I-SceI"
                     /ApEinfo_fwdcolor="#4F0080"
                     /ApEinfo_revcolor="#4F0080"
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gatgacacac attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata
      181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaaggccta ctattagcaa
      781 caacgatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1081 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc
     1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1921 ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac
     1981 ttggttcagg tggagtggga gaagaattac tgacccctcg gggtctcaaa ggcggtttcc
     2041 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
     2101 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
     2161 cttaacccaa ctgcacagaa caaaaacgtg caggaaacga agataaatca tggattctag
     2221 aacagttggt atattaggag ggggacaatt gggacgtatg attgttgagg cagcaaacag
     2281 gctcaacatt aagacggtaa tactagatgc tgaaaattct cctgccaaac aaataagcaa
     2341 ctccaatgac cacgttaatg gctccttttc caatcctctt gatatcgaaa aactagctga
     2401 aaaatgtgat gtgctaacga ttgagattga gcatgttgat gttcctacac taaagaatct
     2461 tcaagtaaaa catcccaaat taaaaattta cccttctcca gaaacaatca gattgataca
     2521 agacaaatat attcaaaaag agcatttaat caaaaatggt atagcagtta cccaaagtgt
     2581 tcctgtggaa caagccagtg agacgtccct attgaatgtt ggaagagatt tgggttttcc
     2641 attcgtcttg aagtcgagga ctttggcata cgatggaaga ggtaacttcg ttgtaaagaa
     2701 taaggaaatg attccggaag ctttggaagt actgaaggat cgtcctttgt acgccgaaaa
     2761 atgggcacca tttactaaag aattagcagt catgattgtg agatctgtta acggtttagt
     2821 gttttcttac ccaattgtag agactatcca caaggacaat atttgtgact tatgttatgc
     2881 gcctgctaga gttccggact ccgttcaact taaggcgaag ttgttggcag aaaatgcaat
     2941 caaatctttt cccggttgtg gtatatttgg tgtggaaatg ttctatttag aaacagggga
     3001 attgcttatt aacgaaattg ccccaaggcc tcacaactct ggacattata ccattgatgc
     3061 ttgcgtcact tctcaatttg aagctcattt gagatcaata ttggatttgc caatgccaaa
     3121 gaatttcaca tctttctcca ccattacaac gaacgccatt atgctaaatg ttcttggaga
     3181 caaacataca aaagataaag agctagaaac ttgcgaaaga gcattggcga ctccaggttc
     3241 ctcagtgtac ttatatggaa aagagtctag acctaacaga aaagtaggtc acataaatat
     3301 tattgcctcc agtatggcgg aatgtgaaca aaggctgaac tacattacag gtagaactga
     3361 tattccaatc aaaatctctg tcgctcaaaa gttggacttg gaagcaatgg tcaaaccatt
     3421 ggttggaatc atcatgggat cagactctga cttgccggta atgtctgccg catgtgcggt
     3481 tttaaaagat tttggcgttc catttgaagt gacaatagtc tctgctcata gaactccaca
     3541 taggatgtca gcatatgcta tttccgcaag caagcgtgga attaaaacaa ttatcgctgg
     3601 agctggtggg gctgctcact tgccaggtat ggtggctgca atgacaccac ttcctgtcat
     3661 cggtgtgccc gtaaaaggtt cttgtctaga tggagtagat tctttacatt caattgtgca
     3721 aatgcctaga ggtgttccag tagctaccgt cgctattaat aatagtacga acgctgcgct
     3781 gttggctgtc agactgcttg gcgcttatga ttcaagttat acaacgaaaa tggaacagtt
     3841 tttattaaag caagaagaag aagttcttgt caaagcacaa aagttagaaa ctgtcggtta
     3901 cgaagcttat ctagaaaaca agtaaaaaac tgtattataa gtaaatgcat gtatactaaa
     3961 ctcacaaatt agagcttcaa tttaattata tcagttatta cccatgatga gaccaatatc
     4021 acgcaaaagc accggcagtc gaaattctcg acgattaggg ataacagggt aatagctgtg
     4081 atccggaatt tgtt
//
