LOCUS       BBF10K_000484           3861 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  OYC pWV01 origin ACTA AAAA in pOpen-v3.
ACCESSION   BBF10K_000484
VERSION     BBF10K_000484.1
KEYWORDS    BBFD_0017; Open Yeast Collection Part; OpenMTA; FreeGenes; Golden
            Gate Assembly.
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
            .
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
REFERENCE   2  (bases 2002 to 3861)
  AUTHORS   Scott Pownall, Open Science Network
  TITLE     Direct Submission
  JOURNAL   https://biobricks.org/freegenes/
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /original_record="pOpen_v3.1"
     primer_bind     complement(24..42)
                     /label="T7 Reverse"
                     /note="/ApEinfo_fwdcolor=#b7e6d7 /ApEinfo_revcolor=#b7e6d7"
                     /original_record="pOpen_v3.1"
     terminator      38..85
                     /label="T7 terminator"
                     /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295"
                     /original_record="pOpen_v3.1"
     primer_bind     complement(86..106)
                     /label="M13 rev"
                     /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd"
                     /original_record="pOpen_v3.1"
     misc_feature    107..121
                     /label="AarI"
                     /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67"
                     /original_record="pOpen_v3.1"
     misc_feature    122..172
                     /label="spacer"
                     /note="/ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67"
                     /original_record="pOpen_v3.1"
     rep_origin      complement(173..761)
                     /direction=LEFT
                     /label="ori"
                     /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc"
                     /original_record="pOpen_v3.1"
     CDS             complement(952..1812)
                     /label="AmpR"
                     /note="/ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW*"
                     /original_record="pOpen_v3.1"
     misc_feature    1918..1932
                     /label="AarI"
                     /note="/ApEinfo_fwdcolor=#d6b295 /ApEinfo_revcolor=#d6b295"
                     /original_record="pOpen_v3.1"
     primer_bind     1933..1949
                     /label="M13 fwd"
                     /note="/ApEinfo_fwdcolor=#84b0dc /ApEinfo_revcolor=#84b0dc"
                     /original_record="pOpen_v3.1"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="/ApEinfo_fwdcolor=#b4abac /ApEinfo_revcolor=#b4abac"
                     /original_record="pOpen_v3.1"
     source          2033..3745
                     /organism="Cloning vector pMQ113"
                     /mol_type="other DNA"
                     /label="shuttle vector"
                     /note="color: #ffffff"
                     /db_xref="taxon:515307"
                     /original_record="part"
     misc_feature    2033..3745
                     /label="pWV01-ori"
                     /note="https://www.ncbi.nlm.nih.gov/nuccore/NC_002192.1
                     http://www.dartmouth.edu/~gotoole/vectors.html"
                     /note="color: #a6acb3"
                     /original_record="part"
     CDS             2339..2548
                     /codon_start=1
                     /transl_table=11
                     /product="OrfB"
                     /label="OrfB"
                     /note="color: #993366"
                     /protein_id="ACB45184.1"
                     /translation="MGGKEANFASVLRPPIKCRVPIFVPKTLYPNWLKGLRGFSIANES
                     PTFSPTFFINLYLSSFIVVFMITK"
                     /original_record="part"
     gene            2589..2750
                     /gene="repC"
                     /label="repC"
                     /note="color: #a6acb3; direction: RIGHT"
                     /original_record="part"
     CDS             2589..2750
                     /codon_start=1
                     /transl_table=11
                     /gene="repC"
                     /product="RepC"
                     /label="repC"
                     /note="color: #993366"
                     /protein_id="ACB45185.1"
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
                     /original_record="part"
     CDS             2817..3515
                     /codon_start=1
                     /transl_table=11
                     /gene="repA-ts"
                     /product="replication protein"
                     /label="repA-ts"
                     /note="color: #993366"
                     /protein_id="ACB45186.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
                     /original_record="part"
     gene            2817..3515
                     /gene="repA-ts"
                     /label="repA-ts"
                     /note="color: #a6acb3; direction: RIGHT"
                     /original_record="part"
     CDS             3512..3661
                     /codon_start=1
                     /transl_table=11
                     /product="OrfD"
                     /label="OrfD"
                     /note="color: #993366"
                     /protein_id="ACB45187.1"
                     /translation="MTNKEKELFAENEELKKEIKDLKERIERYREMEVELSTTIDLLRG
                     GIIE"
                     /original_record="part"
     regulatory      3660..3701
                     /regulatory_class="terminator"
                     /label="putative terminator"
                     /note="color: #ffffff"
                     /original_record="part"
     primer_bind     3746..3769
                     /label="M13 fwd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /original_record="part"
     misc_feature    2002..2021
                     /label="FreeGenes dialout primer"
                     /ApEinfo_fwdcolor="#0048BA"
                     /ApEinfo_revcolor="#0048BA"
     misc_feature    complement(3782..3801)
                     /label="FreeGenes dialout primer"
                     /ApEinfo_fwdcolor="#0048BA"
                     /ApEinfo_revcolor="#0048BA"
     misc_feature    3802..3861
                     /label="FreeGenes barcode"
                     /ApEinfo_fwdcolor="#FF7E00"
                     /ApEinfo_revcolor="#FF7E00"
     protein_bind    join(2022..2027,2029..2032)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    complement(join(3770..3773,3775..3780))
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    3823..3840
                     /label="I-SceI"
                     /ApEinfo_fwdcolor="#4F0080"
                     /ApEinfo_revcolor="#4F0080"
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gatgacacac attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata
      181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaaggccta ctattagcaa
      781 caacgatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1081 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc
     1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1921 ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac
     1981 ttggttcagg tggagtggga gaagaattac tgacccctcg gggtctccac tatttttatt
     2041 aaaattttag cgtttatttc gtttagttat cggcataatc gttaaaacag gcgttatcgt
     2101 agcgtaaaag cccttgagcg tagcgtggct ttgcagcgaa gatgttgtct gttagattat
     2161 gaaagccgat gactgaatga aataataagc gcagcgccct tctatttcgg ttggaggagg
     2221 ctcaagggag tatgagggaa tgaaattccc tcatgggttt gattttaaaa attgcttgca
     2281 attttgccga gcggtagcgc tggaaaattt ttgaaaaaaa tttggaattt ggaaaaaaat
     2341 ggggggaaag gaagcgaatt ttgcttccgt actacgaccc cccattaagt gccgagtgcc
     2401 aatttttgtg ccaaaaacgc tctatcccaa ctggctcaag ggtttaaggg gtttttcaat
     2461 cgccaacgaa tcgccaacgt tttcgccaac gttttttata aatctatatt taagtagctt
     2521 tattgttgtt tttatgatta caaagtgata cactaacttt ataaaattat ttgattggag
     2581 ttttttaaat ggtgatttca gaatcgaaaa aaagagttat gatttctctg acaaaagagc
     2641 aagataaaaa attaacagat atggcgaaac aaaaaggttt ttcaaaatct gcggttgcgg
     2701 cgttagctat agaagaatat gcaagaaagg aatcagaaca aaaaaaataa gcgaaagctc
     2761 gcgtttttag aaggatacga gttttcgcta cttgtttttg ataaggtaat tatatcatgg
     2821 ctattaaaaa tactaaagct agaaattttg gatttttatt atatcctgac tcaattccta
     2881 atgattggaa agaaaaatta gagagtttgg gcgtatctat ggctgtcagt cctttacacg
     2941 atatggacga aaaaaaagat aaagatacat ggaataatag taatattata caaaatggaa
     3001 agcactataa aaaaccacac tatcacgtta tatatattgc acgaaatcct gtaacaatag
     3061 aaagcgttag gaacaagatt aagcgaaaat tggggaatag ttcagttgct catgttgaga
     3121 tacttgatta tatcaaaggt tcatatgaat atttgactca tgaatcaaag gacgctattg
     3181 ctaagaataa acatatatac gacaaaaaag atattttgaa cattaatgat tttgatattg
     3241 accgctatat aacacttgat gaaagccaaa aaagagaatt gaagaattta cttttagata
     3301 tagtggatga ctataatttg gtaaatacaa aagatttaat ggcttttatt cgccttaggg
     3361 gagcggagtt tggaatttta aatacgaatg atgtaaaaga tattgtttca acaaactcta
     3421 gcgcctttag attatggttt gagggcaatt atcagtgtgg atatagagca agttatgcaa
     3481 aggttcttga tgctgaaacg ggggaaataa aatgacaaac aaagaaaaag agttatttgc
     3541 tgaaaatgag gaattaaaaa aagaaattaa ggacttaaaa gagcgtattg aaagatacag
     3601 agaaatggaa gttgaattaa gtacaacaat agatttattg agaggaggga ttattgaata
     3661 aataaaagcc ccctgacgaa agtcgaaggg ggtttttatt ttggtttgat gttgcgatta
     3721 atagcaatac aattgcaata aacaacgcca gggttttccc agtcacgaca aaaggagacc
     3781 aacggagccc ttattgtaac cccccataac ccactgtcgt tatagggata acagggtaat
     3841 atagtatctt caggccgatg a
//
