LOCUS       BsubSecTags_LP_4        4879 bp ds-DNA     linear     17-NOV-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     RBS             50..74
                     /label="YqxM_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       1198..1203
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1013..1018
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             75..203
                     /label="YqxM_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(4085..4090)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             1932..1956
                     /label="YwaD_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       complement(4456..4463)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(1882..1889)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       1882..1889
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             4096..4120
                     /label="YjcM_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       1272..1277
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(1049..1056)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             1098..1122
                     /label="YwmD_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       complement(1661..1668)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(210..215)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3623..3628)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1484..1489
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1921..1926
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       39..44
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(658..665)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       1410..1415
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             2141..2165
                     /label="YpjP_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       complement(2712..2717)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       1049..1056
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             1283..1307
                     /label="YwtD_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       4844..4849
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             295..319
                     /label="YkoJ_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       3091..3096
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(1..8)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(2674..2681)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       3426..3431
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(3053..3060)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             4693..4717
                     /label="SpoIIQ_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             2723..2747
                     /label="TyrA_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       complement(3262..3269)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       1625..1630
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3426..3431)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2527..2532)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3697..3702
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(246..253)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(1234..1241)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             3127..3219
                     /label="WprA_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       284..289
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2527..2532
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(4241..4248)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(2638..2643)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(622..627)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       2092..2099
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             2538..2562
                     /label="DacF_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             4290..4314
                     /label="YhaK_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             926..1006
                     /label="TasA_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(1921..1926)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             3336..3419
                     /label="YbbR_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(2295..2302)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       2056..2061
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       4644..4651
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(1625..1630)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       2489..2496
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             2748..2816
                     /label="TyrA_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             3902..3926
                     /label="PbpD_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             2563..2631
                     /label="DacF_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       2130..2135
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2712..2717
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       2674..2681
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(696..701)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2638..2643
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       246..253
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(4011..4016)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1087..1092
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             901..925
                     /label="TasA_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       complement(404..409)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(39..44)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       210..215
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       890..895
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             320..397
                     /label="YkoJ_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(852..859)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       3660..3666
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(3462..3469)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             514..615
                     /label="Mpr_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(2056..2061)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(1198..1203)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       3053..3060
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             1520..1618
                     /label="YdbK_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(440..447)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(4420..4425)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       1661..1668
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(890..895)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       3853..3860
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(1846..1851)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             2548..2556
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     PmeI_bind       3262..3269
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(4608..4613)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2453..2458)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1846..1851
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3226..3231
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4011..4016
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3500..3505
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4420..4425
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2259..2264)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             1308..1403
                     /label="YwtD_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       1699..1704
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       4456..4463
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             2166..2252
                     /label="YpjP_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             1495..1519
                     /label="YdbK_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       658..665
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(1410..1415)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(1484..1489)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2453..2458
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(478..483)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3017..3022
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(1272..1277)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             4718..4837
                     /label="SpoIIQ_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(2859..2866)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             3708..3732
                     /label="YjiA_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       complement(284..289)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2333..2338
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2897..2902
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2823..2828
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4279..4284
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       852..859
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             2908..2932
                     /label="LytF_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       696..701
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(816..821)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             4530..4601
                     /label="PelB_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(3891..3896)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(4682..4687)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4494..4499
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             3311..3335
                     /label="YbbR_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             2369..2446
                     /label="RpmG_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(1013..1018)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(2489..2496)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       2295..2302
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(2823..2828)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3017..3022)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3817..3822
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             489..513
                     /label="Mpr_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             1735..1839
                     /label="YfkN_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(1446..1453)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(4644..4651)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(3226..3231)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3891..3896
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(4047..4054)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             1710..1734
                     /label="YfkN_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             1957..2049
                     /label="YwaD_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(2092..2099)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       1234..1241
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       3462..3469
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       440..447
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             2344..2368
                     /label="RpmG_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       complement(1699..1704)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             2933..3010
                     /label="LytF_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             4505..4529
                     /label="PelB_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             4121..4198
                     /label="YjcM_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       1446..1453
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       3623..3628
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(4844..4849)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(4494..4499)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3091..3096)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2897..2902)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       404..409
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             3927..4004
                     /label="PbpD_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       4085..4090
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3500..3505)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       478..483
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       2859..2866
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             707..731
                     /label="YpuA_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       4241..4248
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(2333..2338)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             1123..1191
                     /label="YwmD_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(4205..4210)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(3660..3666)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(4279..4284)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       1..8
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(3817..3822)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3300..3305
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4205..4210
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             3511..3535
                     /label="YhjA_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       622..627
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             4315..4413
                     /label="YhaK_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(3853..3860)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             3102..3126
                     /label="WprA_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       complement(3300..3305)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4608..4613
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       816..821
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             3733..3810
                     /label="YjiA_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(2130..2135)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2259..2264
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             732..809
                     /label="YpuA_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(1087..1092)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       4047..4054
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       4682..4687
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             3536..3616
                     /label="YhjA_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(3697..3702)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 GTTTAAACTG GGATCGTCAC TTGATCCCTA GCGAATGTGG TCTCGTACTG GGGGCTAATA
       61 AAGGAGGTTT TTTTATGTTC CGTCTGTTTC ACAATCAGCA GAAAGCGAAA ACTAAGCTGA
      121 AGGTTCTGCT GATTTTCCAG CTTTCCGTTA TCTTTAGCCT GACGGCTGCG ATTTGCCTGC
      181 AGTTCTCCGA CGACACGAGT GCCTCCATGG AGACCTAACC TCTTTAGATT GACCCGAGCG
      241 AACCGGTTTA AACCGCGAAG CAACTATCCC AAAGCAAAAG TGGGGTCTCG TACTGGCTAA
      301 TCAAAAAGGA GGTAAAATAA TGCTGAAGAA GAAGTGGATG GTAGGCTTAC TGGCAGGTTG
      361 CCTGGCAGCT GGCGGTTTCT CCTATAACGC GTTCGCTTCC ATGGAGACCG AACGGGTGAT
      421 CACCGATACA GCCTCACACG TTTAAACGTG TGCATTCTTC GACCTAACTT GCTCTAGGGT
      481 CTCCTACTCA GGCGGCCCAA AGGAGGTATT TTAATGAAAC TGGTTCCTCG TTTTCGTAAA
      541 CAGTGGTTCG CGTATCTTAC TGTCTTATGC CTTGCGTTAG CTGCCGCTGT GTCATTCGGC
      601 GTGCCAGCGA AAGCCTCCAT AGAGACCGAG TGATAAGACC TTTACACCCT GTAGTAAGTT
      661 TAAACGGACA AGGTCCCTAA CAGTCGGTCT TATGCGGTCT CATACTCGGG GAGATAAAAG
      721 GAGGGTTTTT TATGAAGAAA ATCTGGATTG GAATGCTTGC GGCAGCCGTG CTGCTTCTGA
      781 TGGTCCCAAA GGTTAGCCTG GCGGACGCGT CCATAGAGAC CAGCAACTAA CGAGATAGCC
      841 ACTGCGGTCT GGTTTAAACG AACCACGTGG TCACCACGAA ACTGGCTGGG GTCTCCTACT
      901 CGGGGCAAGA AAAGGAGGTA ATTGTATGGG TATGAAGAAG AAACTGTCTC TGGGTGTTGC
      961 ATCTGCGGCA CTTGGCTTGG CGCTGGTGGG TGGCGGTACG TGGGCATCCA TGGAGACCGT
     1021 ACCGTCCGCA GTCCTTTGAT TGGACCTAGT TTAAACCATT CTTGAATCAA CCTTGCGGTA
     1081 GCTTCAGGTC TCTTACTCAC GGGCGAACAA GGAGGTTATA CTATGAAGAA ACTGCTGGCA
     1141 GCCGGAATCA TCGGTCTGTT AACCGTGTCT ATTGCGTCAC CGTCGTTTGC GTCCATCGAG
     1201 ACCCTACTGG ACTTATGACT GGACAGTCTC GCTGTTTAAA CGCATATCCT GTTGTATAGG
     1261 ACCTCAACTT GGGTCTCCTA CTGGGAGCAG TTAAAGGAGG TATTTATATG AACACGTTAG
     1321 CAAACTGGAA GAAGTTCCTG CTGGTCGCCG TCATCATTTG TTTTCTGGTT CCAATCATGA
     1381 CGAAAGCCGA AATCGCCGAA GCATCCATCG AGACCCTGAA CGGGTGCATG CAATAGGTGT
     1441 GCAATGTTTA AACCTCGTCA CTACTTCCCG TGAACAGACC GCAGGTCTCG TACTGGACAA
     1501 CAATAAAGGA GCCTATTTAA TGAAACTGTT TAACCGCAAA GTCACACTGG TAAGTCTGAT
     1561 CTTGATGGCC GTGTTCCAGT TCTTTATGGC TCTGATTATC AAACGTATTG TCATTTCCTC
     1621 CATCGAGACC GCGTCATTGA GCCAGACCCA TAGCGTGACG GTTTAAACGC TAACTTTCTT
     1681 TCGAGCAACG GTCGAGTAGG TCTCTTACTG GCCCACATAA AAGAGCGTCT TTTTATGCGT
     1741 ATCCAGAAAC GTCGCACCCA CGTCGAGAAC ATTCTGCGCA TTCTGTTACC ACCGATTATG
     1801 ATCCTGTCTC TGATTCTGCC TACCCCACCC ATTCACGCTT CCATCGAGAC CAAATACCGA
     1861 CGATGGAAGT GCTAGAGCAA GGTTTAAACC AAGTGGTTCG GAAGGACCAT TCAAGGCCAT
     1921 GGTCTCATAC TTCAGAGGGG TCTGGGAGGT CACGATATGA AGAAACTGCT GACCGTGATG
     1981 ACTATGGCGG TTCTGACTGC AGGCACTCTG CTGCTGCCGG CTCAATCAGT CACCCCAGCC
     2041 GCACATGCTT CCATAGAGAC CTACTGCATG GTCCTAGTTG GATTGGTTTT CGTTTAAACA
     2101 AAGGAGGTAA CTTGAAAGGG CATCAGTCGG GTCTCTTACT CGGCAAGGTC AAAGGAGGTT
     2161 ACAATATGAA ATTGTGGATG CGTAAAACGT TGGTGGTGCT GTTTACGATC GTCACATTCG
     2221 GGTTGGTTTC TCCACCAGCA GCACTGATGG CGTCCATTGA GACCTTGGTG AAAGCGTTGC
     2281 ACTATCTGTG CTTCGTTTAA ACGTATGACG ATCTCACATG CTACCTAGTC GTGGTCTCCT
     2341 ACTCCGGGGG GACAAAGGAG GTTATCGAAT GCGTAAGAAA ATCACGTTGG CTTGCAAGAC
     2401 CTGCGGTAAC CGTAACTATA CCACCATGAA GTCATCGGCA AGCGCATCCA TGGAGACCAT
     2461 CATAGCTTGG AACTGCAACC TGGGATTTGT TTAAACAATG TTCGGTGTTA GGAGCGAAAC
     2521 GTCATAGGTC TCATACTCGC TGGGTAATAA GGAGGTTAAC ATATGAAGCG CTTGTTGAGC
     2581 ACGCTGCTGA TCGGCATTAT GCTGTTGACC TTTGCTCCGT CAGCATTTGC CTCCATCGAG
     2641 ACCGAGTAGA TCCCTTTCCT CTTTCATCGT TCTGTTTAAA CTAAGAGTAT GACGAGCCCT
     2701 CTGACAGACG AGGTCTCATA CTTGAGGGGC CCAAAGGAGG TATTTTTATG AACCAGATGA
     2761 AAGACACTAT TTTGCTGGCC GGTCTGGGCC TGATTGGAGG GAGTATTGCC CTGGCCTCCA
     2821 TCGAGACCTT CCGTCATGCG TGACTGGCTC ACGTTCTGGT TTAAACCAAG GCAAGCACTA
     2881 ACGTTGTACG AATTGGGGTC TCTTACTTAA GAGAAGGAAA GGAGGACATA AGATGAAGAA
     2941 GAAACTGGCA GCTGGTCTGA CAGCAAGCGC TATTGTGGGC ACAACCCTGG TGGTTACTCC
     3001 GGCTGAAGCG TCCATCGAGA CCATAATCAA CGGTAACGCT AGGGTCTTGT AGGTTTAAAC
     3061 AAATGGGGAC TGTGTAAGGA TGCGTCTAAT GGTCTCCTAC TCATAGGGTC GAAAGGAGGT
     3121 CCAATAATGA AGCGCCGTAA ATTCAGCTCA GTTGTAGCAG CTGTGTTGAT CTTTGCGCTG
     3181 ATCTTCTCAC TGTTCAGTCC AGGAACTAAA GCCGCAGCGT CCATTGAGAC CCCTCCGATA
     3241 CAACTGCGCT AGGACACTAA TGTTTAAACG TCCGTCACGA GAGGCTATGT TGGTGAATAG
     3301 GTCTCATACT GACGGAGGAC CGGAAGGATT TTTTTATGGA TAAGTTTCTG AACAACCGTT
     3361 GGGCTGTCAA AATTATTGCT CTGCTGTTTG CGCTGCTGCT GTATGTGGCG GTCAACAGTT
     3421 CCATAGAGAC CGCGTATGTA CTGTAAATCC AGCCAATCGT CGTTTAAACG GGCTTAGCCA
     3481 AGCCGAAGAT GATGAGGCAG GTCTCCTACT GGTAATAAAG GAGCGTTATA TTTTTATGAA
     3541 GAAAGCAGCT GCCGTCCTGT TGAGCTTAGG CCTTGTGTTT GGTTTCTCCT ACGGTGCGGG
     3601 TCACGTTGCT GAAGCATCCA TGGAGACCCG CTTTCGTCAC AGAATGTCGC GTATCCAGGT
     3661 TTAAACGGTG GCCAAAACCA GTCAGAATTG CCACGTGGTC TCATACTCCA GGGGACAAAA
     3721 GGAGGATTAT ATATGGCCGC TCAAACGGAC TACAAGAAAC AAGTCGTGGG CATTCTGCTG
     3781 TCATTGGCCT TCGTCCTGTT CGTCTTTAGT TCCATTGAGA CCGCATATCG TGGGGTTTGG
     3841 CACCTATCGT ACGTTTAAAC ACTGTATGAT ACTGCAATCC CTGCAGCAAG GGTCTCCTAC
     3901 TTGGTAGGTT CAAAGGAGGT ATTTTAATGA CCATGTTGCG TAAAATCATC GGGTGGATTC
     3961 TGCTTCTGTG CATCATTCCC CTGTTTGCCT TTACAGTTAT CGCGTCCATC GAGACCGGTT
     4021 GCGTTGCGGA AGGGATTGTG AGCATCGTTT AAACATACTC CATCAGACAC GGTCCGAGAT
     4081 AACAGGTCTC TTACTGGGAA TACCAAAAGG AGGCCTATAT ATGAAGAAAG AGTTACTTGC
     4141 GTCGCTGGTA CTGTGCCTGT CACTGTCTCC TTTAGTGAGT ACTAACGAAG TCTTTGCGTC
     4201 CATGGAGACC AACTGAGAAC TCAATCCTTC GAGCCTACGT GTTTAAACGT AAGCCCTTGT
     4261 ATAGTAAGCT CAGGGTGGGG TCTCTTACTC AGGCAATAAA AAGCAGGAAA TATCATGCGC
     4321 ACCTGGAAAC GTATTCCCAA GACCACCATG CTGATTAGCC TGGTATCACC GTTTCTGTTA
     4381 ATCACACCAG TGTTGTTCTA CGCAGCCCTG GCGTCCATGG AGACCCGAAC AACATAGATG
     4441 GTTACGGAGT GACTGGTTTA AACTTGTGTG TCGGAGTTTG GGGTGGCTGT GCGGGTCTCG
     4501 TACTTAAGGC GGGAAAAGGA GGTCTAAAAA TGAAACGCCT GTGCCTGTGG TTTACAGTGT
     4561 TTAGCCTGTT CCTGGTTCTG CTGCCAGGTA AAGCATTGGG CTCCATGGAG ACCGGTTCAT
     4621 CTACACCGTG GCACAGTGAG TCAGTTTAAA CTCCAGCGAA GTAAGTACTA AGTATGAGGA
     4681 TGGTCTCTTA CTGGGGTATT ACAAAGGAGG TATTTTAATG CGTGAAGAGG AAAAGAAAAC
     4741 ATCTCAGGTG AAGAAGCTGC AGCAGTTCTT CCGCAAACGT TGGGTGTTTC CGGCAATCTA
     4801 TCTTGTGTCA GCAGCTGTGA TCTTAACAGC GGTTCTGTCC ATTGAGACCT AGACGACTTT
     4861 AGCGAAGTGT GGCAGCCTA
//
