LOCUS       BsubSecTags_LP_6        4868 bp ds-DNA     linear     17-NOV-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     PmeI_bind       complement(2371..2378)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       3381..3388
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(1795..1802)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             2232..2256
                     /label="YwgB_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       complement(3796..3803)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(3834..3839)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       3796..3803
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       1404..1411
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(2147..2152)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(2975..2982)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(3601..3606)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(4074..4079)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(4671..4676)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3601..3606
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(1633..1638)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2335..2340)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             674..698
                     /label="YusW_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       complement(4230..4236)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(183..188)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             1644..1668
                     /label="YwcI_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       2769..2776
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(4194..4199)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2939..2944)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             468..492
                     /label="YuaB_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       4833..4838
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3139..3144
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             3224..3248
                     /label="YwsB_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             2420..2444
                     /label="YwmB_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             1478..1552
                     /label="YvpB_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(3175..3182)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(1028..1035)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             1453..1477
                     /label="YvpB_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             1299..1361
                     /label="YvnB_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       1595..1602
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       1..8
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(872..877)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       625..632
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       3175..3182
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       2939..2944
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       419..426
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(1263..1268)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4597..4602
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       383..388
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             50..74
                     /label="YqzC_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       1759..1764
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4194..4199
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       2371..2378
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(419..426)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             268..292
                     /label="YraJ_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             883..907
                     /label="YvgO_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       2807..2812
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             2026..2050
                     /label="YweA_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       complement(1595..1602)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       3419..3424
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       2571..2578
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       1977..1984
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             699..791
                     /label="YusW_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(3381..3388)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             2257..2328
                     /label="YwgB_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       1795..1802
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       4036..4043
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       2409..2414
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(834..841)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             1077..1101
                     /label="YvgV_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             278..286
                     /label="Shine-Dalgarno sequence"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     Loc             2645..2725
                     /label="YwoF_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       834..841
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       3213..3218
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(3563..3570)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(1941..1946)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4671..4676
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1368..1373
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(663..668)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             3049..3132
                     /label="YwqO_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       3345..3350
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             1274..1298
                     /label="YvnB_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       complement(2571..2578)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             2445..2528
                     /label="YwmB_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(457..462)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       3563..3570
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(437..448)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     PmeI_bind       1028..1035
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             75..176
                     /label="YqzC_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(625..632)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       3760..3765
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3213..3218)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             1844..1868
                     /label="YwdK_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       2015..2020
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(1977..1984)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(2183..2190)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(3345..3350)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(4833..4838)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       1225..1232
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(3760..3765)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(4036..4043)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     PmeI_bind       complement(1..8)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(589..594)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2409..2414)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       4633..4640
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             1869..1934
                     /label="YwdK_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Loc             1669..1752
                     /label="YwcI_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             3430..3454
                     /label="YwtC_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       complement(1759..1764)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1263..1268
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3013..3018)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(257..262)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2535..2540)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       4268..4273
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       663..668
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             908..985
                     /label="YvgO_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             3024..3048
                     /label="YwqO_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             3612..3636
                     /label="YwtF_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             3637..3753
                     /label="YwtF_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       4474..4479
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2609..2614)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(39..44)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       798..803
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2535..2540
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2147..2152
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             3249..3338
                     /label="YwsB_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(1225..1232)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       589..594
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1941..1946
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1559..1564
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2335..2340
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       4436..4443
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(4400..4405)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             4707..4826
                     /label="MotB_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       4400..4405
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(992..997)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3419..3424)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(2769..2776)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(2732..2737)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             493..582
                     /label="YuaB_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             4682..4706
                     /label="MotB_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       4230..4236
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     RBS             4085..4109
                     /label="YxiA_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       complement(2221..2226)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       257..262
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(1559..1564)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             4279..4303
                     /label="YybN_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       complement(2807..2812)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             2620..2644
                     /label="YwoF_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     BsaI_bind       complement(4268..4273)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             1102..1182
                     /label="YvgV_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(4633..4640)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       3834..3839
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1633..1638
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             4510..4590
                     /label="YycP_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       3013..3018
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       2183..2190
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       2221..2226
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       992..997
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       3999..4004
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3527..3532)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             3845..3869
                     /label="YxaK_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     PmeI_bind       complement(219..226)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             2843..2932
                     /label="YwqC_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(4597..4602)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(1066..1071)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1442..1447
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1833..1838
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       complement(4436..4443)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Loc             4304..4393
                     /label="YybN_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             2818..2842
                     /label="YwqC_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             2051..2140
                     /label="YweA_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       complement(1404..1411)
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       3527..3532
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3999..4004)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             3455..3520
                     /label="YwtC_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       39..44
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(383..388)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(4474..4479)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       183..188
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       872..877
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2609..2614
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PmeI_bind       2975..2982
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       4074..4079
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(2015..2020)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(3139..3144)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             4485..4509
                     /label="YycP_RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Loc             3870..3992
                     /label="YxaK_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       457..462
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(1833..1838)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(1442..1447)
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       2732..2737
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             293..376
                     /label="YraJ_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     BsaI_bind       complement(798..803)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1066..1071
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       complement(1368..1373)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     BsaI_bind       1189..1194
                     /label="BsaI_3PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Loc             4110..4187
                     /label="YxiA_SecTag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     PmeI_bind       219..226
                     /label="PmeI_bind"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     BsaI_bind       complement(1189..1194)
                     /label="BsaI_5PrimeBindingSite"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 GTTTAAACAA ATCAACACCC TGTTGCGCAA CGGTGTGGGG TCTCCTACTT GAGAAGGAAA
       61 GGAGGATTTT TTTTATGACC AAACGTGGCA TTCAAGCGTT TGCTGGCGGT ATTATCCTGG
      121 CCACTGCCGT CTTAGCGGCA GTGTTCTATC TGACCGACGA AGATCAGGCT GCTGCGTCCA
      181 TTGAGACCTC TGCGCTATCG GTGATCTCGT GGGTTGGAGT TTAAACAGCA GATAAGGGAA
      241 CAGTAGGTCC ACGATAGGTC TCCTACTTGG TACAAACTAA GGAGGTTTTA CTATGACCCT
      301 GACCAAACTG AAAATGCTGT CCATGCTGAC TGTGATGATT GCAAGCCTGT TCATCTTTAG
      361 CTCACAGGCG CTGGCGTCCA TGGAGACCTA CCTCCCTAGC ATTACAAGTC TTAGCAATGT
      421 TTAAACGCAC TGGGCATCGT CCTTCAATGC GTTGAGGGTC TCCTACTTGA GTACTTCAAA
      481 GGAGAATTTT TAATGAAACG TAAACTGCTG TCTAGCCTGG CGATCTCTGC ACTGTCACTG
      541 GGCTTGCTTG TGAGCGCACC CACTGCGTCC TTCGCTGCCG AGTCCATTGA GACCGACCGG
      601 AACTGAACAG GTAGGGTTTC CGGTGTTTAA ACACATTGCG TCTTTCTGCC GAATTGGCAG
      661 AGGGTCTCCT ACTGATTTCC CAAAGGACCC TTTTTTTAAT GCATCTGATT CGTGCTGCAG
      721 GCGCTGTCTG CTTAGCGGTA GTACTGATCG CGGGTTGTCG TTTTAACGAG GACCAACATC
      781 AGGCGGAAGG CTCCATCGAG ACCGAAAAGT GGTGCAGTGC GGTCGTGCTG ATTGTTTAAA
      841 CTCGCCATCA TAGAGGACTT GGGATGTTCG AGGTCTCCTA CTTCTGGGGG GCAAAGGAGG
      901 TATTTTTATG AAACGCATCC GTATTCCGAT GACGTTGGCT TTAGGTGCAG CATTAACGAT
      961 TGCGCCTTTA TCGTTTGCCA GTGCCTCCAT GGAGACCCTT CTCTGACATC CCATAAGCAA
     1021 CATGCGGGTT TAAACGGCTG TCGCACATCC CGAATAGAGG AAGCGGGTCT CGTACTTAAG
     1081 GCGGCGAAGG TAGGTATTTA TATGAAGAAG AAACAGCAGA GCAGCGCTAA GTTTGCCGTG
     1141 ATTTTGACCG TTGTGGTGGT GGTCTTGCTG GCTGCAATAG TATCCATAGA GACCTCCCTA
     1201 ACAACTATGT AAGGGGTAGG ATTTGTTTAA ACGCCCGAAA CGTGAGTACT GCAGACTGCT
     1261 CGGGTCTCGT ACTAGAACCA TTGAAAGGAG GTCTTTTAAT GCGTAAGTAT ACCGTGATTG
     1321 CCTCTATCCT GTTGTCGTTC CTTAGCGTCC TTTCTGGCGG TTCCATTGAG ACCCAGAATA
     1381 AGAACGGAGC GTGAATGTAT TGGGTTTAAA CTCCGTAGGA ACCGAAACAC AGACTCTCCA
     1441 GGGTCTCTTA CTTCTTGGTT TCGAAGGAGG TCTTGATATG AAAACCCTGC GTACGTTGTG
     1501 TGTGTTGATG ATCCTTTCCG GTGTGATCTT CTTTGGACTG AAAATCGACG CCTCCATTGA
     1561 GACCCCCACG ACATATGCAG GCAATCTGCA TCGAGTTTAA ACGACTCTAA TCGCTGTTGT
     1621 AGACCACGGC TTGGTCTCAT ACTCCGGGGG AAAGGAGGTT TATTTTTTAT GAAACGCCTG
     1681 TTGGTTAGCT TACGCGTGTG GATGGTTTTC TTAATGAACT GGGTTACGCC AGACCGCAAA
     1741 ACAGCACGTG CATCCATGGA GACCCCGTAT GATCGGAAAC GCTTCCGCTC CAAAGTTTAA
     1801 ACCCAAACTC TCCGCTGCGG AAAGTGAATC CGGGTCTCAT ACTCGGGGGA AGTAAAGGAG
     1861 GTTTTTTTAT GAAAGTGTTC ATCATCCTTG GTGCGATCAA CGCCTTGTTG GCAGTGGGCC
     1921 TGGGCGCATT CGGTTCCATG GAGACCGGAA TGCTGATGGA CTATGAGACA GCATCAGTTT
     1981 AAACATTCTA CCTCGCTACC GACTGGCAGC CTACGGTCTC TTACTGGGGC TAACGAAAGG
     2041 AGGTTTAATT ATGTTGAAAC GCACCAGCTT TGTCAGCAGT CTGTTTATCT CCTCCGCAGT
     2101 CCTGCTTTCT ATTTTGCTGC CGTCTGGCCA GGCACATGCA TCCATGGAGA CCATGCCCAA
     2161 GTAGGGAGGC TCTAAGCACA TTGTTTAAAC CTAGAGTTGC CACGGGATTA GTCTCTATCG
     2221 GGTCTCATAC TCTGGGTAAG AGCAGGAGGA TTATTTATGA AGATGAAATC AGGTATGGAA
     2281 CAAGCCGTGA GCGTGCTGTT ACTGCTGAGT CGCCTTCCCG TTCAGGCGTC CATTGAGACC
     2341 AAGATAGGAT TACCGAATGC GTCGCTTGAA GTTTAAACCT CCGTATGGCA GTCTGATACC
     2401 GCGAAGCAGG TCTCGTACTC AGGGGGAAGA GAGGAGGTAA AGTAATGAAG AAGAAACAGG
     2461 TGTCTCACGC CATCATCATC TCTGTCATGC TGTCCTTTGT CATTGCTGTT TTCCACACGA
     2521 TTCATGCTTC CATGGAGACC TTAAGACCGT ACGAAGCATG CTCGTACGGT GTTTAAACGT
     2581 TTTCGCTGTC TGATTGGCGA TCGGGTTCGG TCTCCTACTC CAGGGGGGAA AAGGAGGTAT
     2641 ATTAATGCGT AAGTGGTATT TCATCTTGCT GGCAGGGGTG CTGACTTCTG TAATCTTGGC
     2701 CTTTGTTTAC GACAAAACGA AAGCATCCAT CGAGACCAGC TGGCAGAGGG TTTAGGAACA
     2761 GCGAGACTGT TTAAACTCCT TCCTCCCAAC GCTAAGGTGA CGTATCGGTC TCCTACTCCG
     2821 GGGAATTAAA GGAGGTTTAT ATATGGGCGA GAGTACAAGC CTGAAAGAGA TCCTGAGTAC
     2881 GTTGACCAAA CGCATCCTGC TGATTATGAT TGTCACTGCG GCAGCAACGG CTTCCATGGA
     2941 GACCTCTTAG GCTCAGTAAG CTATGCATCC AAAGGTTTAA ACGTGTGTAC GTACTGATAC
     3001 CTTGATGTCA ATGGTCTCGT ACTTGCGAGG GGTAAAGGAG GTTTTTCTAT GAAGTTTCTG
     3061 CTGTCCGTGA TTGCAGGATT ACTGATTCTG GCCCTGTACC TGTTTTGGAA AGTCCAACCG
     3121 CCTGTATGGA TTTCCATCGA GACCGGGTTT GTTTCGGTGG GTCTGCTTGT TGACGTTTAA
     3181 ACCGAGATCG AAACGCTTAT GGTCGCTAGA CTGGTCTCCT ACTCCGGGGG TCTAAAGGAG
     3241 GTTTTTATAT GAATAAGCCC ACGAAGTTAT TCTCTACGCT GGCTCTGGCA GCCGGTATGA
     3301 CGGCTGCTGC AGCAGGAGGC GCAGGGACTA TTCATGCATC CATTGAGACC GGCCACTGGA
     3361 CCTGTTCCTT ATGGTTAAGG GTTTAAACCC AAGTACGGAG TCACCCAGTA ATCGCAGCGG
     3421 TCTCCTACTC TGGGGGCTGA AAGGAGGTTT TTTTATGAAG TTCGTGAAAG CGATCTGGCC
     3481 ATTTGTGGCG GTGGCCATTG TGTTCATGTT CATGTCTGCT TCCATCGAGA CCCAGATATG
     3541 CTGGGTGCCT GCTAAAGTGA CAGTTTAAAC TACTGGTAGA TAACGGTGAG ACTCTACGGA
     3601 GGTCTCGTAC TCAGGAATCA AAAAGGAGAC TATTTTATGG AAGAACGCTC CCAACGCCGT
     3661 AAGAAGAAAC GCAAGCTGAA GAAGTGGGTC AAGGTTGTTG CTGGCCTTAT GGCGTTTCTT
     3721 GTTATCGCGG CAGGCAGTGT GGGTGCATAT GCATCCATGG AGACCGAAGC AGTCATAGAC
     3781 TTCGCAATGT GAGCAGTTTA AACTCCTCTT AGGCAGGGCT TACATTCCAC ATTGGTCTCA
     3841 TACTCTGGGA GGGGAAAGGA GGTAGTTTGA TGGTGAAGTC TTTTCGTATG AAAGCGCTGA
     3901 TCGCTGGAGC CGCAGTGGCC GCAGCGGTGT CGGCTGGAGC TGTCTCTGAT GTTCCGGCTG
     3961 CTAAAGTGCT GCAACCTACA GCAGCTTACG CGTCCATAGA GACCATGGTT TCTTGATGTA
     4021 ACGGCAGCAC TCAACGTTTA AACGAATCAG TCAGGTGATA CCTAGAGACT CGGGGTCTCA
     4081 TACTCGGGCC ACCCTTTGGA GGTATTTTTA TGTTTAACCG CCTGTTCCGC GTCTGCTTTC
     4141 TTGCCGCACT GATCATGGCT TTTACACTGC CGAACAGCGT GTACGCCTCC ATGGAGACCC
     4201 ACCCATCACA CACGCTCCAA GTCGTTACTG TTTAAATCAG CGTCGCAAGG GACAAAGCCA
     4261 TTGTGCTGGT CTCATACTGA ATCATAATAA AGGAGGTTTA TTTATGAACA AGTTCCTGAA
     4321 AAGCAACTTC CGCTTCCTGC TGGCCGCAGC GCTGGGGATT AGCCTGCTGG CCAGCTCGAA
     4381 CTTCATCAAA GCGTCCATAG AGACCGGTTT GTCGCTGCAC TGGGAATTGT CACTGGTTTA
     4441 AACTTTTCTC AGGTACAGAG GATCTCTCGA AAAGGTCTCC TACTTGCGGG CCCGCCAGGA
     4501 GGTACAAGGA TGAAGAAATG GATGATCACG ATTGCTATGC TGATTCTGGC GGGAATCGCT
     4561 CTGTTTGTGT TTATCTCACC ACTGAAGTCA TCCATGGAGA CCATCTCGTA CGCGATTTCC
     4621 CGAGGGTTCC CAGTTTAAAC ACAGATAGTT ATCAAGCTCC GGACCTACCG GGTCTCTTAC
     4681 TGATAAAATA GAAAGGAGCT TTTTTTATGG CGCGTAAGAA GAAGAAGAAA CACGAAGATG
     4741 AACATGTTGA CGAGTCCTGG TTGGTGCCGT ACGCGGATAT CCTTACGCTG CTTCTGGCGT
     4801 TGTTCATCGT ATTGTATGCC TCTTCGTCCA TAGAGACCCA ACATTACACG GGAGACAAGT
     4861 CATAGAGT
//
