LOCUS       pBad-Xis/Int_Reset_Flip 5693 bp ds-DNA     circular     12-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    124..145
                     /label="Biobrick prefix (Bba)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(146..1223)
                     /label="AraC"
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_fwdcolor=#ff0080
     misc_feature    1224..1353
                     /label="pBad"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1360..1384
                     /label="RBS-50000"
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_fwdcolor=#cc0000
     misc_feature    1385..2165
                     /label="Bxb1-Excisionase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2171..2184
                     /label="RBS B0031"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    2191..3693
                     /label="Bxb1 Integrase"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3694..3714
                     /label="Biobrick suffix (BBa)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(4003..4338)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(4359..4705)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4830..5491)
                     /label="Chloramphenicol Resistance"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
       61 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
      121 ctggaattcg cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt
      181 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      241 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      301 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      361 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      421 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      481 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      541 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      601 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      661 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      721 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      781 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      841 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      901 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      961 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1021 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1081 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1141 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1201 tctataatca cggcagaaaa gtcacattga ttatttgcac ggcgtcacac tttgctatgc
     1261 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
     1321 tactgtttct ccataccgtt tttttgggct agcTACTAGA CCTTAACAAA CTACGGAGGT
     1381 CAACatggcc ggcactcagc gtatcgtctt tctacccgat actcagttgc ctttcgaggc
     1441 gcgcaaagag atgcaagcgg tcatccgctt catcggggat gtccagccgt acggcgtggt
     1501 acatatcggt gacgtcctag acctgccgca gccctcgcgc tggaaccggg gaaccaaggg
     1561 cgagttcgag ggctcggtgt accgcgacgc ggactacgcc aagaagaacc tgatggagcc
     1621 gctgcgcaag gtctacgacg gctggatcgg gatgcacgag ggcaaccacg atctgcgagc
     1681 ccgcgagtac ctggccaaga acgcaccggc cctggagggt acgcacgctt tcgacatcga
     1741 cgtgctgctc gacttcgacg ggttcggtgt ggagctgctg cctgacttct acgacatcgc
     1801 tccgggctgg atctccactc acgggcacat gggcaagatg acgctatccc agatcgccgg
     1861 atcgacagcg ctcaacggtg ccaagaagtt cggcaagtcc gtggtctgcg gccacacgca
     1921 ccggcaggct gtcgtctcgc actcgttcgg gtacggcggc tcggtgcgca agaccgtcac
     1981 cggcatggaa gtcgggcacc tgatggacat gaagaaggcc aactatctaa agggcggagc
     2041 cgggaactgg cagatgggct tcgggatgct cacggtcgac ggcaagcatg tcaaggctga
     2101 gatcgtcccg atcctgggag gcaagttcac cgttgacggc caggtctggg aagtcaccgg
     2161 tTAAtactag tcacacagga aacctactag ATGAGAGCCC TGGTAGTCAT CCGCCTGTCC
     2221 CGCGTCACCG ATGCTACGAC TTCACCGGAG CGTCAGCTGG AGTCTTGCCA GCAGCTCTGC
     2281 GCCCAGCGCG GCTGGGACGT CGTCGGGGTA GCGGAGGATC TGGACGTCTC CGGGGCGGTC
     2341 GATCCGTTCG ACCGGAAGCG CAGACCGAAC CTGGCCCGGT GGCTAGCGTT CGAGGAGCAA
     2401 CCGTTTGACG TGATCGTGGC GTACCGGGTA GATCGGTTGA CCCGATCGAT CCGGCATCTT
     2461 CAGCAGCTGG TCCACTGGGC CGAGGACCAC AAGAAGCTGG TCGTCTCCGC GACCGAAGCG
     2521 CACTTCGATA CGACGACGCC GTTTGCGGCG GTCGTCATCG CGCTTATGGG AACGGTGGCG
     2581 CAGATGGAAT TAGAAGCGAT CAAAGAGCGG AACCGTTCGG CTGCGCATTT CAATATCCGC
     2641 GCCGGGAAAT ACCGAGGATC CCTGCCGCCG TGGGGATACC TGCCTACGCG CGTGGACGGG
     2701 GAGTGGCGGC TGGTGCCGGA CCCTGTGCAG CGAGAGCGCA TCCTCGAGGT GTATCACCGC
     2761 GTCGTCGACA ACCACGAGCC GCTGCATCTG GTGGCCCACG ACCTGAACCG GCGTGGTGTC
     2821 CTGTCGCCGA AGGACTACTT CGCGCAGCTG CAAGGCCGCG AGCCGCAGGG CCGGGAGTGG
     2881 TCGGCTACCG CGCTGAAGCG ATCGATGATC TCCGAGGCGA TGCTCGGGTA CGCGACTCTG
     2941 AACGGTAAGA CCGTCCGAGA CGACGACGGA GCCCCGCTGG TGCGGGCTGA GCCGATCCTG
     3001 ACCCGTGAGC AGCTGGAGGC GCTGCGCGCC GAGCTCGTGA AGACCTCCCG GGCGAAGCCC
     3061 GCGGTGTCTA CCCCGTCGCT GCTGCTGCGG GTGTTGTTCT GCGCGGTGTG CGGGGAGCCC
     3121 GCGTACAAGT TCGCCGGGGG AGGACGTAAG CACCCGCGCT ACCGCTGCCG CTCGATGGGG
     3181 TTCCCGAAGC ACTGCGGGAA CGGCACGGTG GCGATGGCCG AGTGGGACGC GTTCTGCGAG
     3241 GAGCAGGTAC TGGATCTGCT CGGGGACGCG GAGCGTCTGG AGAAAGTCTG GGTAGCGGGC
     3301 TCGGACTCCG CGGTCGAACT CGCGGAGGTG AACGCGGAGC TGGTGGACCT GACGTCGCTG
     3361 ATCGGCTCCC CGGCCTACCG GGCGGGCTCT CCGCAGCGAG AAGCACTGGA TGCCCGTATT
     3421 GCGGCGCTGG CCGCGCGGCA AGAGGAGCTG GAGGGCCTGG AGGCTCGCCC GTCTGGCTGG
     3481 GAGTGGCGCG AGACCGGGCA GCGGTTCGGG GACTGGTGGC GGGAGCAGGA CACCGCGGCA
     3541 AAGAACACCT GGCTTCGGTC GATGAACGTT CGGCTGACGT TCGACGTCCG CGGCGGGCTG
     3601 ACTCGCACGA TCGACTTCGG GGATCTTCAG GAGTACGAGC AGCATCTCAG GCTCGGCAGC
     3661 GTGGTCGAAC GGCTACACAC CGGGATGTCG taaTACTAGT AGCGGCCGCT GCAGtccggc
     3721 aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt
     3781 cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3841 gcggtatcag ctcactcaaa ggcggtaatc ctagggcgtt cggctgcggc gagcggtatc
     3901 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3961 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4201 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4321 ctatcgtctt gagtccaatg ccacctgacg tctaagaacc cggtaagaca cgacttatcg
     4381 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4441 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     4501 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4561 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4621 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4681 tcacgttaag ggattttggt catgacttgt gcttggattc tcaccaataa aaaacgcccg
     4741 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     4801 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
     4861 actgttgtaa ttcattaagc attctgccga catggaagcc atcacagacg gcatgatgaa
     4921 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     4981 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     5041 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     5101 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     5161 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     5221 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     5281 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     5341 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     5401 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     5461 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     5521 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     5581 ttacgtgccg atcaacgtct cattttcgcc agatatcgac gtctaagaaa ccattattat
     5641 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc acc
//