LOCUS       SR_latch_G8-C1          7483 bp ds-DNA     circular     12-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    124..145
                     /label="prefix Bba"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    146..199
                     /label="pTetO"
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_fwdcolor=#006666
     misc_feature    206..230
                     /label="6N-RBS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    231..233
                     /label="GTG Start"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    231..1733
                     /label="SET Integrase"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1754..1848
                     /label="B0014"
                     /ApEinfo_revcolor=#cc3300
                     /ApEinfo_fwdcolor=#cc3300
     misc_feature    complement(1871..2948)
                     /label="AraC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2949..3078
                     /label="pBad"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    3085..3109
                     /label="RBS-50000"
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_fwdcolor=#cc0000
     misc_feature    3110..3886
                     /label="Bxb1-Excisonase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3887..3919
                     /label="AAK Tag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3920..3922
                     /label="Bxb1-Excisonase"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3939..3952
                     /label="RBS B0031"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    3959..3961
                     /label="GTG Start"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3959..5503
                     /label="RESET Integrase"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5465..5503
                     /label="ARH Tag"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5504..5524
                     /label="suffix BBa"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(5813..6495)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    6620..7281
                     /label="Chloramphenicol Resistance"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
       61 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
      121 ctgGAATTCG CGGCCGCTTC TAGAGTCCCT ATCAGTGATA GAGATTGACA TCCCTATCAG
      181 TGATAGAGAT ACTGAGCACA AGCTTGCGGC TTTCACACTT GGGGGCTAGC GTGAGAGCCC
      241 TGGTAGTCAT CCGCCTGTCC CGCGTCACCG ATGCTACGAC TTCACCGGAG CGTCAGCTGG
      301 AGTCTTGCCA GCAGCTCTGC GCCCAGCGCG GCTGGGACGT CGTCGGGGTA GCGGAGGATC
      361 TGGACGTCTC CGGGGCGGTC GATCCGTTCG ACCGGAAGCG CAGACCGAAC CTGGCCCGGT
      421 GGCTAGCGTT CGAGGAGCAA CCGTTTGACG TGATCGTGGC GTACCGGGTA GATCGGTTGA
      481 CCCGATCGAT CCGGCATCTT CAGCAGCTGG TCCACTGGGC CGAGGACCAC AAGAAGCTGG
      541 TCGTCTCCGC GACCGAAGCG CACTTCGATA CGACGACGCC GTTTGCGGCG GTCGTCATCG
      601 CGCTTATGGG AACGGTGGCG CAGATGGAAT TAGAAGCGAT CAAAGAGCGG AACCGTTCGG
      661 CTGCGCATTT CAATATCCGC GCCGGGAAAT ACCGAGGATC CCTGCCGCCG TGGGGATACC
      721 TGCCTACGCG CGTGGACGGG GAGTGGCGGC TGGTGCCGGA CCCTGTGCAG CGAGAGCGCA
      781 TCCTCGAGGT GTATCACCGC GTCGTCGACA ACCACGAGCC GCTGCATCTG GTGGCCCACG
      841 ACCTGAACCG GCGTGGTGTC CTGTCGCCGA AGGACTACTT CGCGCAGCTG CAAGGCCGCG
      901 AGCCGCAGGG CCGGGAGTGG TCGGCTACCG CGCTGAAGCG ATCGATGATC TCCGAGGCGA
      961 TGCTCGGGTA CGCGACTCTG AACGGTAAGA CCGTCCGAGA CGACGACGGA GCCCCGCTGG
     1021 TGCGGGCTGA GCCGATCCTG ACCCGTGAGC AGCTGGAGGC GCTGCGCGCC GAGCTCGTGA
     1081 AGACCTCCCG GGCGAAGCCC GCGGTGTCTA CCCCGTCGCT GCTGCTGCGG GTGTTGTTCT
     1141 GCGCGGTGTG CGGGGAGCCC GCGTACAAGT TCGCCGGGGG AGGACGTAAG CACCCGCGCT
     1201 ACCGCTGCCG CTCGATGGGG TTCCCGAAGC ACTGCGGGAA CGGCACGGTG GCGATGGCCG
     1261 AGTGGGACGC GTTCTGCGAG GAGCAGGTAC TGGATCTGCT CGGGGACGCG GAGCGTCTGG
     1321 AGAAAGTCTG GGTAGCGGGC TCGGACTCCG CGGTCGAACT CGCGGAGGTG AACGCGGAGC
     1381 TGGTGGACCT GACGTCGCTG ATCGGCTCCC CGGCCTACCG GGCGGGCTCT CCGCAGCGAG
     1441 AAGCACTGGA TGCCCGTATT GCGGCGCTGG CCGCGCGGCA AGAGGAGCTG GAGGGCCTGG
     1501 AGGCTCGCCC GTCTGGCTGG GAGTGGCGCG AGACCGGGCA GCGGTTCGGG GACTGGTGGC
     1561 GGGAGCAGGA CACCGCGGCA AAGAACACCT GGCTTCGGTC GATGAACGTT CGGCTGACGT
     1621 TCGACGTCCG CGGCGGGCTG ACTCGCACGA TCGACTTCGG GGATCTTCAG GAGTACGAGC
     1681 AGCATCTCAG GCTCGGCAGC GTGGTCGAAC GGCTACACAC CGGGATGTCG taagcggccg
     1741 cATGCATTAA TAAtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1801 agagagaata taaaaagcca gattattaat ccggcttttt tattatttCC TGCAGGgcgg
     1861 ccgcCATATG ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
     1921 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
     1981 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
     2041 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
     2101 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
     2161 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
     2221 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
     2281 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
     2341 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
     2401 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
     2461 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
     2521 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
     2581 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
     2641 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
     2701 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
     2761 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
     2821 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     2881 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     2941 gaaaagtcac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
     3001 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
     3061 ccgttttttt gggctagcTA CTAGACCTTA ACAAACTACG GAGGTCAACa tggccggcac
     3121 tcagcgtatc gtctttctac ccgatactca gttgcctttc gaggcgcgca aagagatgca
     3181 agcggtcatc cgcttcatcg gggatgtcca gccgtacggc gtggtacata tcggtgacgt
     3241 cctagacctg ccgcagccct cgcgctggaa ccggggaacc aagggcgagt tcgagggctc
     3301 ggtgtaccgc gacgcggact acgccaagaa gaacctgatg gagccgctgc gcaaggtcta
     3361 cgacggctgg atcgggatgc acgagggcaa ccacgatctg cgagcccgcg agtacctggc
     3421 caagaacgca ccggccctgg agggtacgca cgctttcgac atcgacgtgc tgctcgactt
     3481 cgacgggttc ggtgtggagc tgctgcctga cttctacgac atcgctccgg gctggatctc
     3541 cactcacggg cacatgggca agatgacgct atcccagatc gccggatcga cagcgctcaa
     3601 cggtgccaag aagttcggca agtccgtggt ctgcggccac acgcaccggc aggctgtcgt
     3661 ctcgcactcg ttcgggtacg gcggctcggt gcgcaagacc gtcaccggca tggaagtcgg
     3721 gcacctgatg gacatgaaga aggccaacta tctaaagggc ggagccggga actggcagat
     3781 gggcttcggg atgctcacgg tcgacggcaa gcatgtcaag gctgagatcg tcccgatcct
     3841 gggaggcaag ttcaccgttg acggccaggt ctgggaagtc accggtgcag caaacgacga
     3901 aaactacgct gctGCTAAAt aataaGGCGC GCCtagagtc acacaggaaa cctactagGT
     3961 GAGAGCCCTG GTAGTCATCC GCCTGTCCCG CGTCACCGAT GCTACGACTT CACCGGAGCG
     4021 TCAGCTGGAG TCTTGCCAGC AGCTCTGCGC CCAGCGCGGC TGGGACGTCG TCGGGGTAGC
     4081 GGAGGATCTG GACGTCTCCG GGGCGGTCGA TCCGTTCGAC CGGAAGCGCA GACCGAACCT
     4141 GGCCCGGTGG CTAGCGTTCG AGGAGCAACC GTTTGACGTG ATCGTGGCGT ACCGGGTAGA
     4201 TCGGTTGACC CGATCGATCC GGCATCTTCA GCAGCTGGTC CACTGGGCCG AGGACCACAA
     4261 GAAGCTGGTC GTCTCCGCGA CCGAAGCGCA CTTCGATACG ACGACGCCGT TTGCGGCGGT
     4321 CGTCATCGCG CTTATGGGAA CGGTGGCGCA GATGGAATTA GAAGCGATCA AAGAGCGGAA
     4381 CCGTTCGGCT GCGCATTTCA ATATCCGCGC CGGGAAATAC CGAGGATCCC TGCCGCCGTG
     4441 GGGATACCTG CCTACGCGCG TGGACGGGGA GTGGCGGCTG GTGCCGGACC CTGTGCAGCG
     4501 AGAGCGCATC CTCGAGGTGT ATCACCGCGT CGTCGACAAC CACGAGCCGC TGCATCTGGT
     4561 GGCCCACGAC CTGAACCGGC GTGGTGTCCT GTCGCCGAAG GACTACTTCG CGCAGCTGCA
     4621 AGGCCGCGAG CCGCAGGGCC GGGAGTGGTC GGCTACCGCG CTGAAGCGAT CGATGATCTC
     4681 CGAGGCGATG CTCGGGTACG CGACTCTGAA CGGTAAGACC GTCCGAGACG ACGACGGAGC
     4741 CCCGCTGGTG CGGGCTGAGC CGATCCTGAC CCGTGAGCAG CTGGAGGCGC TGCGCGCCGA
     4801 GCTCGTGAAG ACCTCCCGGG CGAAGCCCGC GGTGTCTACC CCGTCGCTGC TGCTGCGGGT
     4861 GTTGTTCTGC GCGGTGTGCG GGGAGCCCGC GTACAAGTTC GCCGGGGGAG GACGTAAGCA
     4921 CCCGCGCTAC CGCTGCCGCT CGATGGGGTT CCCGAAGCAC TGCGGGAACG GCACGGTGGC
     4981 GATGGCCGAG TGGGACGCGT TCTGCGAGGA GCAGGTACTG GATCTGCTCG GGGACGCGGA
     5041 GCGTCTGGAG AAAGTCTGGG TAGCGGGCTC GGACTCCGCG GTCGAACTCG CGGAGGTGAA
     5101 CGCGGAGCTG GTGGACCTGA CGTCGCTGAT CGGCTCCCCG GCCTACCGGG CGGGCTCTCC
     5161 GCAGCGAGAA GCACTGGATG CCCGTATTGC GGCGCTGGCC GCGCGGCAAG AGGAGCTGGA
     5221 GGGCCTGGAG GCTCGCCCGT CTGGCTGGGA GTGGCGCGAG ACCGGGCAGC GGTTCGGGGA
     5281 CTGGTGGCGG GAGCAGGACA CCGCGGCAAA GAACACCTGG CTTCGGTCGA TGAACGTTCG
     5341 GCTGACGTTC GACGTCCGCG GCGGGCTGAC TCGCACGATC GACTTCGGGG ATCTTCAGGA
     5401 GTACGAGCAG CATCTCAGGC TCGGCAGCGT GGTCGAACGG CTACACACCG GGATGTCGAC
     5461 CGGCGCAGCA AACGACGAAA ACTACGCTGC TCGTCATTAA TAATACTAGT AGCGGCCGCT
     5521 GCAGtccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     5581 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5641 gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc ctagggcgtt cggctgcggc
     5701 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5761 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5821 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5881 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5941 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6001 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     6061 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6121 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6181 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6241 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6301 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6361 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6421 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6481 ggattttggt catgacttgt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     6541 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     6601 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     6661 ttcattaagc attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc
     6721 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     6781 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     6841 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     6901 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     6961 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     7021 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     7081 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     7141 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     7201 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     7261 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     7321 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     7381 atcaacgtct cattttcgcc agatatcgac gtctaagaaa ccattattat catgacatta
     7441 acctataaaa ataggcgtat cacgaggccc tttcgtcttc acc
//