LOCUS       AND_gate_v2.0           5042 bp ds-DNA     circular     12-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label="prefix Bba"
                     /ApEinfo_revcolor=#4c4c4c
                     /ApEinfo_fwdcolor=#4c4c4c
     misc_feature    23..39
                     /label="P7 promoter"
                     /ApEinfo_revcolor=#008040
                     /ApEinfo_fwdcolor=#008040
     misc_feature    57..69
                     /label="P7 promoter"
                     /ApEinfo_revcolor=#008040
                     /ApEinfo_fwdcolor=#008040
     misc_feature    76..128
                     /label="ATTB-TP901"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    129..257
                     /label="B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    complement(258..307)
                     /label="attP TP901"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    314..363
                     /label="attB Bxb1"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    364..476
                     /label="J61048"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    complement(477..529)
                     /label="attP Bxb1"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    530..604
                     /label="RiboJ"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    605..691
                     /label="BCD2_apFAB682"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    693..1409
                     /label="Superfolder registry"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1418..1546
                     /label="B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1547..1567
                     /label="suffix BBa"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1662..1681
                     /label="VR primer "
                     /ApEinfo_revcolor=#4c4c4c
                     /ApEinfo_fwdcolor=#4c4c4c
     misc_feature    1730..3766
                     /label="pSC101"
                     /ApEinfo_revcolor=#666666
                     /ApEinfo_fwdcolor=#666666
     misc_feature    3775..4717
                     /label="Amp resistance"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4797..4816
                     /label="VF2 primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gaattcgcgg ccgcttctag agAAAAAATT TATTTGCTTt cgcatctttt tgtaccTATA
       61 ATGTGTGGAA AGCTTATGCC AACACAATTA ACATCTCAAT CAAGGTAAAT GCTTTTTGCT
      121 TTTTTTGCcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      181 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      241 gcctttctgc gtttataaaa ggagtttttt agttacctta attgaaataa acgaaataaa
      301 aactcgcGCT AGCTCGGCCG GCTTGTCGAC GACGGCGGTC TCCGTCGTCA GGATCATCCG
      361 GGCccggctt atcggtcagt ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt
      421 gcttttggag gggcagaaag atgaatgact gtccacgacg ctatacccaa aagaaaGGGT
      481 TTGTACCGTA CACCACTGAG ACCGCGGTGG TTGACCAGAC AAACCACGAA GCTGTCACCG
      541 GATGTGCTTT CCGGTCTGAT GAGTCCGTGA GGACGAAACA GCCTCTACAA ATAATTTTGT
      601 TTAAGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA
      661 CATCTTAATC ATGCTAAGGA GGTTTTCTAA Tgcgtaaagg cgaagagctg ttcactggtg
      721 tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg
      781 gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc tgtactactg
      841 gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt gttcagtgct
      901 ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc atgccggaag
      961 gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa acgcgtgcgg
     1021 aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc attgacttta
     1081 aagaagacgg caatatcctg ggccataagc tggaatacaa ttttaacagc cacaatgttt
     1141 acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg
     1201 tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca atcggtgatg
     1261 gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg tctaaagatc
     1321 cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg ggcatcacgc
     1381 atggtatgga tgaactgtac aaatgatgat actagagcca ggcatcaaat aaaacgaaag
     1441 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1501 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataTACT AGTAGCGGCC
     1561 GCTGCAGgag tcactaaggg ttagttagtt agattagcag aaagtcaaaa gcctccgacc
     1621 ggaggctttt gactaaaact tcccttgggg ttatcattgg ggctcactca aaggcggtaa
     1681 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctgctagagc tgtcagacca
     1741 agtttacgag ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     1801 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagca
     1861 ctagggacag taagacgggt aagcctgttg atgataccgc tgccttactg ggtgcattag
     1921 ccagtctgaa tgacctgtca cgggataatc cgaagtggtc agactggaaa atcagagggc
     1981 aggaactgct gaacagcaaa aagtcagata gcaccacata gcagacccgc cataaaacgc
     2041 cctgagaagc ccgtgacggg cttttcttgt attatgggta gtttccttgc atgaatccat
     2101 aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag cgcagcgaac
     2161 tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa aaggaatatt
     2221 cagcgatttg cccgagcttg cgagggtgct acttaagcct ttagggtttt aaggtctgtt
     2281 ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa ctgactaaag
     2341 tagtgagtta tacacagggc tgggatctat tctttttatc tttttttatt ctttctttat
     2401 tctataaatt ataaccactt gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt
     2461 tacagagggt ctagcagaat ttacaagttt tccagcaaag gtctagcaga atttacagat
     2521 acccacaact caaaggaaaa ggacatgtaa ttatcattga ctagcccatc tcaattggta
     2581 tagtgattaa aatcacctag accaattgag atgtatgtct gaattagttg ttttcaaagc
     2641 aaatgaacta gcgattagtc gctatgactt aacggagcat gaaaccaagc taattttatg
     2701 ctgtgtggca ctactcaacc ccacgattga aaaccctaca aggaaagaac ggacggtatc
     2761 gttcacttat aaccaatacg ctcagatgat gaacatcagt agggaaaatg cttatggtgt
     2821 attagctaaa gcaaccagag agctgatgac gagaactgtg gaaatcagga atcctttggt
     2881 taaaggcttt gagattttcc agtggacaaa ctatgccaag ttctcaagcg aaaaattaga
     2941 attagttttt agtgaagaga tattgcctta tcttttccag ttaaaaaaat tcataaaata
     3001 taatctggaa catgttaagt cttttgaaaa caaatactct atgaggattt atgagtggtt
     3061 attaaaagaa ctaacacaaa agaaaactca caaggcaaat atagagatta gccttgatga
     3121 atttaagttc atgttaatgc ttgaaaataa ctaccatgag tttaaaaggc ttaaccaatg
     3181 ggttttgaaa ccaataagta aagatttaaa cacttacagc aatatgaaat tggtggttga
     3241 taagcgaggc cgcccgactg atacgttgat tttccaagtt gaactagata gacaaatgga
     3301 tctcgtaacc gaacttgaga acaaccagat aaaaatgaat ggtgacaaaa taccaacaac
     3361 cattacatca gattcctacc tacgtaacgg actaagaaaa acactacacg atgctttaac
     3421 tgcaaaaatt cagctcacca gttttgaggc aaaatttttg agtgacatgc aaagtaagca
     3481 tgatctcaat ggttcgttct catggctcac gcaaaaacaa cgaaccacac tagagaacat
     3541 actggctaaa tacggaagga tctgaggttc ttatggctct tgtatctatc agtgaagcat
     3601 caagactaac aaacaaaagt agaacaactg ttcaccgtta gatatcaaag ggaaaactgt
     3661 ccatatgcac agatgaaaac ggtgtaaaaa agatagatac atcagagctt ttacgagttt
     3721 ttggtgcatt taaagctgtt caccatgaac agatcgacaa tgtaactact agagctaaat
     3781 acattcaaat atctatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3841 aaaaaggaag aatatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3901 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgccgaaga
     3961 tcagttgggt gcacgtgtgg gttacatcga actggacctc aacagcggta agattcttga
     4021 gagttttcgc cccgaagaac gtttcccaat gatgagcact tttaaagttc tgctctgtgg
     4081 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4141 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg acggcatgac
     4201 agtacgcgaa ttatgcagcg ctgccataac catgagtgat aacacggcgg ccaacttact
     4261 tctgacaacg atcggaggac cgaaggagct taccgctttt ttgcacaaca tgggtgatca
     4321 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4381 tgacaccacg atgcctgtag ctatggcaac aacgttgcgc aaactcttaa ctggcgaact
     4441 tcttactctc gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4501 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4561 tgagcgtggg tcccgcggta ttattgcagc cctggggcca gatggtaagc cctcccgtat
     4621 cgtagttatc tacacgacgg ggagccaggc aactatggac gaacgtaatc gccagatcgc
     4681 tgagataggt gcctccctga ttaagcattg gtaataatac tagctccggc aaaaaaacgg
     4741 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgtttgcc
     4801 acctgacgtc taagaaaagg aatattcagc aatttgcccg tgccgaagaa aggcccaccc
     4861 gtgaaggtga gccagtgagt tgattgctac gtaattagtt agttagccct tagtgactct
     4921 gccacctgac gtctaagaaa aggaatattc agcaatttgc ccgtgccgaa gaaaggccca
     4981 cccgtgaagg tgagccagtg agttgattgc tacgtaatta gttagttagc ccttagtgac
     5041 tc
//