LOCUS       OR_gate                 4808 bp ds-DNA     circular     12-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label="prefix Bba"
                     /ApEinfo_revcolor=#333333
                     /ApEinfo_fwdcolor=#333333
     misc_feature    23..39
                     /label="P7 promoter"
                     /ApEinfo_revcolor=#008040
                     /ApEinfo_fwdcolor=#008040
     misc_feature    57..69
                     /label="P7 promoter"
                     /ApEinfo_revcolor=#008040
                     /ApEinfo_fwdcolor=#008040
     misc_feature    76..128
                     /label="ATTB-TP901"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    129..178
                     /label="attB Bxb1"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    179..307
                     /label="B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    308..360
                     /label="attP Bxb1"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    361..410
                     /label="attP TP901"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    428..455
                     /label="JBEI RBS"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    456..1175
                     /label="Superfolder GFP"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    1184..1312
                     /label="B0015"
                     /ApEinfo_revcolor=#666666
                     /ApEinfo_fwdcolor=#666666
     misc_feature    1313..1333
                     /label="suffix BBa"
                     /ApEinfo_revcolor=#333333
                     /ApEinfo_fwdcolor=#333333
     misc_feature    1428..1447
                     /label="VR primer "
                     /ApEinfo_revcolor=#4c4c4c
                     /ApEinfo_fwdcolor=#4c4c4c
     misc_feature    1496..3532
                     /label="pSC101"
                     /ApEinfo_revcolor=#666666
                     /ApEinfo_fwdcolor=#666666
     misc_feature    3541..4483
                     /label="Amp resistance"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4563..4582
                     /label="VF2 primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gaattcgcgg ccgcttctag agAAAAAATT TATTTGCTTt cgcatctttt tgtaccTATA
       61 ATGTGTGGAA AGCTTATGCC AACACAATTA ACATCTCAAT CAAGGTAAAT GCTTTTTGCT
      121 TTTTTTGCTC GGCCGGCTTG TCGACGACGG CGGTCTCCGT CGTCAGGATC ATCCGGGCcc
      181 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      241 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      301 gtttataTCG TGGTTTGTCT GGTCAACCAC CGCGGTCTCA GTGGTGTACG GTACAAACCC
      361 gcgagttttt atttcgttta tttcaattaa ggtaactaaa aaactccttt GGCGCGCCTA
      421 CTAGAGAAAA GATCTTTTAA GAAGGAGATA TACATatgcg taaaggcgaa gagctgttca
      481 ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg
      541 tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta
      601 ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact tatggtgttc
      661 agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc
      721 cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc
      781 gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg
      841 actttaaaga agacggcaat atcctgggcc ataagctgga atacaatttt aacagccaca
      901 atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc
      961 acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg
     1021 gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta
     1081 aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca
     1141 tcacgcatgg tatggatgaa ctgtacaaat gatgatacta gagccaggca tcaaataaaa
     1201 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1261 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagta
     1321 gcggccgctg caggagtcac taagggttag ttagttagat tagcagaaag tcaaaagcct
     1381 ccgaccggag gcttttgact aaaacttccc ttggggttat cattggggct cactcaaagg
     1441 cggtaatcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgc tagagctgtc
     1501 agaccaagtt tacgagctcg cttggactcc tgttgataga tccagtaatg acctcagaac
     1561 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
     1621 caagcactag ggacagtaag acgggtaagc ctgttgatga taccgctgcc ttactgggtg
     1681 cattagccag tctgaatgac ctgtcacggg ataatccgaa gtggtcagac tggaaaatca
     1741 gagggcagga actgctgaac agcaaaaagt cagatagcac cacatagcag acccgccata
     1801 aaacgccctg agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga
     1861 atccataaaa ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca
     1921 gcgaactgaa tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg
     1981 aatattcagc gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg
     2041 tctgttttgt agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga
     2101 ctaaagtagt gagttataca cagggctggg atctattctt tttatctttt tttattcttt
     2161 ctttattcta taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc
     2221 ggaatttaca gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt
     2281 acagataccc acaactcaaa ggaaaaggac atgtaattat cattgactag cccatctcaa
     2341 ttggtatagt gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt
     2401 caaagcaaat gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat
     2461 tttatgctgt gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac
     2521 ggtatcgttc acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta
     2581 tggtgtatta gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc
     2641 tttggttaaa ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa
     2701 attagaatta gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat
     2761 aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga
     2821 gtggttatta aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct
     2881 tgatgaattt aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa
     2941 ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt
     3001 ggttgataag cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca
     3061 aatggatctc gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc
     3121 aacaaccatt acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc
     3181 tttaactgca aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag
     3241 taagcatgat ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga
     3301 gaacatactg gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg
     3361 aagcatcaag actaacaaac aaaagtagaa caactgttca ccgttagata tcaaagggaa
     3421 aactgtccat atgcacagat gaaaacggtg taaaaaagat agatacatca gagcttttac
     3481 gagtttttgg tgcatttaaa gctgttcacc atgaacagat cgacaatgta actactagag
     3541 ctaaatacat tcaaatatct atccgctcat gagacaataa ccctgataaa tgcttcaata
     3601 atattgaaaa aggaagaata tgagtattca acatttccgt gtcgccctta ttcccttttt
     3661 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3721 cgaagatcag ttgggtgcac gtgtgggtta catcgaactg gacctcaaca gcggtaagat
     3781 tcttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct
     3841 ctgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3901 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggacgg
     3961 catgacagta cgcgaattat gcagcgctgc cataaccatg agtgataaca cggcggccaa
     4021 cttacttctg acaacgatcg gaggaccgaa ggagcttacc gcttttttgc acaacatggg
     4081 tgatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4141 cgagcgtgac accacgatgc ctgtagctat ggcaacaacg ttgcgcaaac tcttaactgg
     4201 cgaacttctt actctcgctt cccggcaaca attaatagac tggatggagg cggataaagt
     4261 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4321 agccggtgag cgtgggtccc gcggtattat tgcagccctg gggccagatg gtaagccctc
     4381 ccgtatcgta gttatctaca cgacggggag ccaggcaact atggacgaac gtaatcgcca
     4441 gatcgctgag ataggtgcct ccctgattaa gcattggtaa taatactagc tccggcaaaa
     4501 aaacgggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg
     4561 tttgccacct gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc
     4621 ccacccgtga aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt
     4681 gactctgcca cctgacgtct aagaaaagga atattcagca atttgcccgt gccgaagaaa
     4741 ggcccacccg tgaaggtgag ccagtgagtt gattgctacg taattagtta gttagccctt
     4801 agtgactc
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