LOCUS       Dual_controller_plasmid 6613 bp ds-DNA     circular     12-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label="prefix Bba"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    23..76
                     /label="pTetO"
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_fwdcolor=#006666
     misc_feature    77..107
                     /label="RBS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    108..1610
                     /label="Bxb1 Integrase"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1631..1725
                     /label="B0014"
                     /ApEinfo_revcolor=#cc3300
                     /ApEinfo_fwdcolor=#cc3300
     misc_feature    complement(1748..2825)
                     /label="AraC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2826..2955
                     /label="pBad"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2962..2977
                     /label="RBS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2984..4510
                     /label="6his-TP901  integrase"
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_fwdcolor=#ff00ff
     misc_feature    2990..3008
                     /label="6-His tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4511..4531
                     /label="suffix BBa"
                     /ApEinfo_revcolor=#000000
                     /ApEinfo_fwdcolor=#000000
     rep_origin      complement(4820..5502)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5627..6288
                     /label="Chloramphenicol Resistance"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 GAATTCGCGG CCGCTTCTAG AGTCCCTATC AGTGATAGAG ATTGACATCC CTATCAGTGA
       61 TAGAGATACT GAGCACAAGC TTGCGGCTTT CACACTTGGG GGCTAGCGTG AGAGCCCTGG
      121 TAGTCATCCG CCTGTCCCGC GTCACCGATG CTACGACTTC ACCGGAGCGT CAGCTGGAGT
      181 CTTGCCAGCA GCTCTGCGCC CAGCGCGGCT GGGACGTCGT CGGGGTAGCG GAGGATCTGG
      241 ACGTCTCCGG GGCGGTCGAT CCGTTCGACC GGAAGCGCAG ACCGAACCTG GCCCGGTGGC
      301 TAGCGTTCGA GGAGCAACCG TTTGACGTGA TCGTGGCGTA CCGGGTAGAT CGGTTGACCC
      361 GATCGATCCG GCATCTTCAG CAGCTGGTCC ACTGGGCCGA GGACCACAAG AAGCTGGTCG
      421 TCTCCGCGAC CGAAGCGCAC TTCGATACGA CGACGCCGTT TGCGGCGGTC GTCATCGCGC
      481 TTATGGGAAC GGTGGCGCAG ATGGAATTAG AAGCGATCAA AGAGCGGAAC CGTTCGGCTG
      541 CGCATTTCAA TATCCGCGCC GGGAAATACC GAGGATCCCT GCCGCCGTGG GGATACCTGC
      601 CTACGCGCGT GGACGGGGAG TGGCGGCTGG TGCCGGACCC TGTGCAGCGA GAGCGCATCC
      661 TCGAGGTGTA TCACCGCGTC GTCGACAACC ACGAGCCGCT GCATCTGGTG GCCCACGACC
      721 TGAACCGGCG TGGTGTCCTG TCGCCGAAGG ACTACTTCGC GCAGCTGCAA GGCCGCGAGC
      781 CGCAGGGCCG GGAGTGGTCG GCTACCGCGC TGAAGCGATC GATGATCTCC GAGGCGATGC
      841 TCGGGTACGC GACTCTGAAC GGTAAGACCG TCCGAGACGA CGACGGAGCC CCGCTGGTGC
      901 GGGCTGAGCC GATCCTGACC CGTGAGCAGC TGGAGGCGCT GCGCGCCGAG CTCGTGAAGA
      961 CCTCCCGGGC GAAGCCCGCG GTGTCTACCC CGTCGCTGCT GCTGCGGGTG TTGTTCTGCG
     1021 CGGTGTGCGG GGAGCCCGCG TACAAGTTCG CCGGGGGAGG ACGTAAGCAC CCGCGCTACC
     1081 GCTGCCGCTC GATGGGGTTC CCGAAGCACT GCGGGAACGG CACGGTGGCG ATGGCCGAGT
     1141 GGGACGCGTT CTGCGAGGAG CAGGTACTGG ATCTGCTCGG GGACGCGGAG CGTCTGGAGA
     1201 AAGTCTGGGT AGCGGGCTCG GACTCCGCGG TCGAACTCGC GGAGGTGAAC GCGGAGCTGG
     1261 TGGACCTGAC GTCGCTGATC GGCTCCCCGG CCTACCGGGC GGGCTCTCCG CAGCGAGAAG
     1321 CACTGGATGC CCGTATTGCG GCGCTGGCCG CGCGGCAAGA GGAGCTGGAG GGCCTGGAGG
     1381 CTCGCCCGTC TGGCTGGGAG TGGCGCGAGA CCGGGCAGCG GTTCGGGGAC TGGTGGCGGG
     1441 AGCAGGACAC CGCGGCAAAG AACACCTGGC TTCGGTCGAT GAACGTTCGG CTGACGTTCG
     1501 ACGTCCGCGG CGGGCTGACT CGCACGATCG ACTTCGGGGA TCTTCAGGAG TACGAGCAGC
     1561 ATCTCAGGCT CGGCAGCGTG GTCGAACGGC TACACACCGG GATGTCGtaa gcggccgcAT
     1621 GCAgTAATAA tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga
     1681 gagaatataa aaagccagat tattaatccg gcttttttat tatttCCTGC AGGgcggccg
     1741 cCATATGtta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga
     1801 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt
     1861 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct
     1921 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc
     1981 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat
     2041 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat
     2101 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa
     2161 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt
     2221 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat
     2281 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa
     2341 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg
     2401 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca
     2461 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga
     2521 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat
     2581 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc
     2641 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct
     2701 tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac
     2761 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa
     2821 aagtcacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
     2881 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccg
     2941 tttttttggg ctagctacta gagtcacaca ggaaacctac tagATGAAAC ATCATCACCA
     3001 TCACCACCAG GCCGGCacta agaaagtagc aatctataca cgagtatcca ctactaacca
     3061 agcagaggaa ggcttctcaa ttgatgagca aattgaccgt ttaacaaaat atgctgaagc
     3121 aatggggtgg caagtatctg atacttatac tgatgctggt ttttcagggg ccaaacttga
     3181 acgcccagca atgcaaagat taatcaacga tatcgagaat aaagcttttg atacagttct
     3241 tgtatataag ctagaccgcc tttcacgtag tgtaagagat actctttatc ttgttaagga
     3301 tgtgttcaca aaaaataaaa tagactttat ctcgcttaat gaaagtattg atacttcttc
     3361 tgctatgggt agcttgtttc tcactattct ttctgcaatt aatgagtttg aaagagagaa
     3421 tataaaagaa cgcatgacta tgggtaaact agggcgagcg aaatctggta agtctatgat
     3481 gtggactaag acagcttttg ggtattacca caacagaaag acaggtatat tagaaattgt
     3541 tcctttacaa gctacaatag ttgaacaaat attcactgat tatttatcag gaatatcact
     3601 tacaaaatta agagataaac tcaatgaatc tggacacatc ggtaaagata taccgtggtc
     3661 ttatcgtacc ctaagacaaa cacttgataa tccagtttac tgtggttata tcaaatttaa
     3721 ggacagccta tttgaaggta tgcacaaacc aattatccct tatgagactt atttaaaagt
     3781 tcaaaaagag ctagaagaaa gacaacagca gacttatgaa agaaataaca accctagacc
     3841 tttccaagct aaatatatgc tgtcagggat ggcaaggtgc ggttactgtg gagcaccttt
     3901 aaaaattgtt cttggccaca aaagaaaaga tggaagccgc actatgaaat atcactgtgc
     3961 aaatagattt cctcgaaaaa caaaaggaat tacagtatat aatgacaata aaaagtgtga
     4021 ttcaggaact tatgatttaa gtaatttaga aaatactgtt attgacaacc tgattggatt
     4081 tcaagaaaat aatgactcct tattgaaaat tatcaatggc aacaaccaac ctattcttga
     4141 tacttcgtca tttaaaaagc aaatttcaca gatcgataaa aaaatacaaa agaactctga
     4201 tttgtaccta aatgatttta tcactatgga tgagttgaaa gatcgtactg attcccttca
     4261 ggctgagaaa aagctgctta aagctaagat tagcgaaaat aaatttaatg actctactga
     4321 tgtttttgag ttagttaaaa ctcagttggg ctcaattccg attaatgaac tatcatatga
     4381 taataaaaag aaaatcgtca acaaccttgt atcaaaggtt gatgttactg ctgataatgt
     4441 agatatcata tttaaattcC AACTCGCTAC CGGTGCTGCT AAGGACGAAA ACTACGCTCT
     4501 GGCTGCTTAA TACTAGTAGC GGCCGCTGCA Gtccggcaaa aaaacgggca aggtgtcacc
     4561 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
     4621 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4681 ggtaatccta gggcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gacttgtgct tggattctca
     5521 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     5581 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     5641 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     5701 acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     5761 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     5821 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     5881 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     5941 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     6001 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     6061 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     6121 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     6181 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     6241 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     6301 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     6361 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaga tatcgacgtc
     6421 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     6481 cgtcttcacc tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     6541 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
     6601 aggatgattt ctg
//