LOCUS       Exported                5277 bp DNA     circular SYN 02-JUL-2021
DEFINITION  BBF10K_000511 in pOpen_v4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
COMMENT     This file was created using tools provided by Twist Bioscience
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="BBF10K_000511 in pOpen_v4"
     primer_bind     21..51
                     /label=Gibson-for
     regulatory      46..55
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             52..783
                     /codon_start=1
                     /product="UL16 binding protein 1 isoform 1"
                     /label=ULBP1I1
                     /translation="MAAAASPAFLLCLPLLHLLSGWSRAGWVDTHCLCYDFIITPKSRP
                     EPQWCEVQGLVDERPFLHYDCVNHKAKAFASLGKKVNVTKTWEEQTETLRDVVDFLKGQ
                     LLDIQVENLIPIEPLTLQARMSCEHEAHGHGRGSWQFLFNGQKFLLFDSNNRKWTALHP
                     GAKKMTEKWEKNRDVTMFFQKISLGDCKMWLEEFLMYWEQMLDPTKPPSLAPGTTQPKA
                     MATTLSPWSLLIIFLCFILAGR"
     primer_bind     complement(784..808)
                     /label=Gibson-rev
     terminator      927..974
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(979..995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1154..1404
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(1407..2162)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      2253..2472
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    2892..2913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2928..2958
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2966..2982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2990..3006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3079..3924
                     /codon_start=1
                     /label=repL
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCAN
                     LAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANLYR
                     FTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSPCQD
                     DVAIKNKKSQVKKTKRSVSGGAGTTSLKKLTSWIAKAKAKADNLRLSKKRTQKHEFKQK
                     VEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIPAGFRG
                     "
     misc_feature    4032..4183
                     /label=nnpp promoters
     CDS             4223..5038
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      complement(5148..5191)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     5209..5225
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      5226..5257
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aagaattact gacccctcgg cagactgagt cgcccggggg gatccgccac catggccgca
       61 gcagcttccc ctgcttttct cttgtgtttg cctctcctgc atctcctctc cggatggtct
      121 cgcgctggat gggtcgatac acattgtctc tgttatgatt ttataataac acctaaatcc
      181 cgacctgaac cacaatggtg tgaagtacaa gggcttgttg atgaaaggcc tttcttgcat
      241 tatgattgtg tcaatcataa agctaaagca tttgcgtctc tcggaaagaa agtcaatgtc
      301 accaaaactt gggaagaaca aactgaaact cttcgggatg ttgtagattt tcttaaaggg
      361 cagcttctgg atattcaagt tgaaaatctc ataccaatag aaccacttac attgcaagct
      421 cgcatgtctt gtgaacatga agcacatgga catgggcgcg ggtcttggca atttctgttt
      481 aatggacaaa agtttctcct tttcgattct aataatcgca aatggacggc tctccatcct
      541 ggtgcaaaga aaatgactga gaagtgggaa aagaataggg atgttacaat gttctttcag
      601 aaaatttccc tgggtgattg caaaatgtgg ttggaagaat ttcttatgta ttgggaacag
      661 atgctcgatc ctactaaacc accaagtctt gctcctggta caactcaacc aaaagctatg
      721 gcgacaacgc tctcaccttg gagtctcctc attattttcc tctgttttat cctggccggc
      781 agaggatccg ccaccaactt cagcctgcta gacacaaggc tgattccagt tagtaggcag
      841 tataaccgag tagggataac agggtaatgc ggcccaaccg ggctctgtcc gctgaggtgt
      901 caatcgtcgg agccgctgag caataactag cataacccct tggggcctct aaacgggtct
      961 tgaggggttt tttgcatggt catagctgtt tcctgagagc ttggcaggtg tcgcgtatgc
     1021 atcgatcacg tgtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1081 ggatcgatcc ggcgcgcacc gtgggaaaaa ctccaggtag aggtacacac gcggatagcc
     1141 aattcagagt aataaactgt gataatcaac cctcatcaat gatgacgaac taacccccga
     1201 tatcaggtca catgacgaag ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa
     1261 tttggcttcc ttaaaaatta cagttcaaaa agtatgagaa aatccatgca ggctgaagga
     1321 aacagcaaaa ctgtgacaaa ttaccctcag taggtcagaa caaatgtgac gaaccaccct
     1381 caaatctgtg acagataacc ctcagactat cctgtcgtca tggaagtgat atcgcggaag
     1441 gaaaatacga tatgagtcgt ctggcggcct ttctttttct caatgtatga gaggcgcatt
     1501 ggagttctgc tgttgatctc attaacacag acttgcagga agcggcggcg gaagtcaggc
     1561 atacgctggt aactttgagg cagctggtaa cgctctatga tccagtcgat tttcagagac
     1621 acgatgcctg agccatccgg cttacgatac tgacacaggg attcgtataa acgcatggca
     1681 tacggattgg tgatttcttt tgtttcacta agccgaaact gcgtaaaccg gttctgtaac
     1741 ccgataaaga agggaatgag atatgggttg atatgtacac tgtaaagccc tctggatgga
     1801 ctgtgcgcac gtttgataaa ccaaggaaaa gattcatagc ctttttcatc accggcatcc
     1861 tcttcagggc gataaaaaac cacttccttc cccgcgaaac tcttcaatgc ctgccgtata
     1921 tccttactgg cttccgcaga ggtcaatccg aatatttcag catatttagc aacatggatc
     1981 tcgcagatac cgtcatgttc ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac
     2041 agatacagca tacgtttttg atcccgggag agactatatg ccgcctcagt gaggtcgttt
     2101 gactggacga ttcgcgggct atttttacgt ttcttgtgat tgataaccgc tgtttccgcc
     2161 atgacagatc catgtgaagt gtgacaagtt tttagattgt cacactaaat aaaaaagagt
     2221 caataagcag ggataacttt gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg
     2281 ttaagggcaa tttgtcacag acaggactgt catttgaggg tgatttgtca cactgaaagg
     2341 gcaatttgtc acaacacctt ctgtagaacc agcatggata aaggcctaca aggcgctcta
     2401 aaaaagaaga tctaaaaact ataaaaaaaa taattataaa aatatccccg tggataagtg
     2461 gataacccca agggaagttt tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt
     2521 ccctggtgct tcctcgctca ctcgaaattc ccggggatag ctttatgctt gtaaaccgtt
     2581 ttgtgaaaaa atttttaaaa taaaaaaggg gacctctagg gtccccaatt aattagtaat
     2641 ataatctatt aaaggtcatt caaaaggtca tccaccggat caattcccct gctcgcgcag
     2701 gctgggtgcc aagctctcgg gtaacatcaa ggcccgatcc ttggagccct tgccctcccg
     2761 cacgatgatc gtgccgtgat cgaaatccag atccttgacc cgcagttgca aaccctcact
     2821 gatccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2881 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     2941 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     3001 tatgaccatg attacgccaa gcgcggtacc tgtcgcggca acgcgctaac agacgtagta
     3061 agaaccacca gcattgtaat gctggctaaa gtcactttcc tgagctgtat aacgatgagc
     3121 gattttactt tttctggcta tgaattggcc tgctttgtaa cacactccgg tctatcccgt
     3181 agcgccgggc atatcctgtc gcaatgtgca aatctcgcgg caacaaccag tgaatacttc
     3241 attcacaagc ctcaccgcct gatcgcggca gaaactggtt atagccaatc aaccgtcgtt
     3301 cgtgcattcc gtgaagctgt aaacaaaggt atcctgtctg tagagattgt tatcggcgat
     3361 caccgtgaac gtcgcgctaa cctgtaccgg tttacaccat cctttttggc cttcgcacaa
     3421 caagccaaaa atgcgctgat agaaagcaaa ttaaagatct cttcagcggc aaccaaggtt
     3481 aaagctgttc tcgctaagac attggcttta tttaattttt tatccacacc cccatgtcaa
     3541 aatgataccc cctccccctg tcaggatgac gtggcaataa agaataagaa gtcacaagtt
     3601 aaaaaaacaa aaagatcagt ttccggcggt gccggaacaa ccagcctcaa aaaattgact
     3661 tcatggatcg ctaaggcaaa agcaaaggct gacaatctgc ggttatccaa aaaacgcact
     3721 caaaaacatg agttcaagca gaaagtagag gcggctgcgc ggaaatatgc ttacctgaag
     3781 aacaagcgtt cgcctgatat tggcgggata tcaaacttcg ataacctacc gcattgcatg
     3841 acggtaaacg aagctcttaa tgcggtttta gccaaaaata aagataacga acaatggggt
     3901 ataccggcag gattcagagg gtaatgaatt gctctaatta taaccatgca tactttcaac
     3961 acctctagtt tgccatgagg caaactcata ggtgtcctgg taagaggaca ctgttgccaa
     4021 aactggacgc cccattattg caattaataa acaactaacg gacaattcta cctaacaata
     4081 agtggagttg cggccggccg cgatccgcta gcaaagccac gttgtgtctc aaaatctctg
     4141 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     4201 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgcgccc
     4261 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     4321 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
     4381 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     4441 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     4501 tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata
     4561 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     4621 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca
     4681 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     4741 gcctgttgaa caagtctgga aagaaatgca taaactgttg ccattctcac cggattcagt
     4801 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     4861 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     4921 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     4981 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     5041 agaattggtt aattggttgt aacactggcg agctctgcca cctgacgtct aagaaaccat
     5101 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     5161 aaaatcctta gctttcgcta aggatgattt ctgcacctgc accagtcagt aaaacgacgg
     5221 ccagtagtca aaagcctccg accggaggct tttgacttgg ttcaggtgga gtgggag
//
