LOCUS       Exported                4737 bp DNA     circular SYN 02-JUL-2021
DEFINITION  BBF10K_000512 in pOpen_v4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
COMMENT     This file was created using tools provided by Twist Bioscience
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="BBF10K_000512 in pOpen_v4"
     primer_bind     21..51
                     /label=Gibson-for
     regulatory      46..55
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             52..243
                     /codon_start=1
                     /product="UL16 binding protein 1 isoform 2"
                     /label=ULBP1I2
                     /translation="MAAAASPAFLLCLPLLHLLSGWSRAGWVEPPSLAPGTTQPKAMAT
                     TLSPWSLLIIFLCFILAGR"
     primer_bind     complement(244..268)
                     /label=Gibson-rev
     terminator      387..434
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(439..455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    614..864
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(867..1622)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      1713..1932
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    2352..2373
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2388..2418
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2426..2442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2450..2466
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2539..3384
                     /codon_start=1
                     /label=repL
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCAN
                     LAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANLYR
                     FTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSPCQD
                     DVAIKNKKSQVKKTKRSVSGGAGTTSLKKLTSWIAKAKAKADNLRLSKKRTQKHEFKQK
                     VEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIPAGFRG
                     "
     misc_feature    3492..3643
                     /label=nnpp promoters
     CDS             3683..4498
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      complement(4608..4651)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     4669..4685
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4686..4717
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aagaattact gacccctcgg cagactgagt cgcccggggg gatccgccac catggccgca
       61 gctgcatccc ctgcatttct cttgtgtctc ccactccttc atctcctttc cggatggtct
      121 agggcaggtt gggttgaacc gccatccctt gcacctggta ctacgcaacc taaagctatg
      181 gctacgactc tctcaccgtg gtctttgctg atcatcttcc tgtgcttcat cctggccggc
      241 agaggatccg ccaccaactt cagcctgcta gacatgggat tgaccacact gacgctatct
      301 cggctaagaa tagggataac agggtaatgg attgaccgcc gggcagcgac gctgaggtgt
      361 caatcgtcgg agccgctgag caataactag cataacccct tggggcctct aaacgggtct
      421 tgaggggttt tttgcatggt catagctgtt tcctgagagc ttggcaggtg tcgcgtatgc
      481 atcgatcacg tgtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
      541 ggatcgatcc ggcgcgcacc gtgggaaaaa ctccaggtag aggtacacac gcggatagcc
      601 aattcagagt aataaactgt gataatcaac cctcatcaat gatgacgaac taacccccga
      661 tatcaggtca catgacgaag ggaaagagaa ggaaatcaac tgtgacaaac tgccctcaaa
      721 tttggcttcc ttaaaaatta cagttcaaaa agtatgagaa aatccatgca ggctgaagga
      781 aacagcaaaa ctgtgacaaa ttaccctcag taggtcagaa caaatgtgac gaaccaccct
      841 caaatctgtg acagataacc ctcagactat cctgtcgtca tggaagtgat atcgcggaag
      901 gaaaatacga tatgagtcgt ctggcggcct ttctttttct caatgtatga gaggcgcatt
      961 ggagttctgc tgttgatctc attaacacag acttgcagga agcggcggcg gaagtcaggc
     1021 atacgctggt aactttgagg cagctggtaa cgctctatga tccagtcgat tttcagagac
     1081 acgatgcctg agccatccgg cttacgatac tgacacaggg attcgtataa acgcatggca
     1141 tacggattgg tgatttcttt tgtttcacta agccgaaact gcgtaaaccg gttctgtaac
     1201 ccgataaaga agggaatgag atatgggttg atatgtacac tgtaaagccc tctggatgga
     1261 ctgtgcgcac gtttgataaa ccaaggaaaa gattcatagc ctttttcatc accggcatcc
     1321 tcttcagggc gataaaaaac cacttccttc cccgcgaaac tcttcaatgc ctgccgtata
     1381 tccttactgg cttccgcaga ggtcaatccg aatatttcag catatttagc aacatggatc
     1441 tcgcagatac cgtcatgttc ctgtagggtg ccatcagatt ttctgatctg gtcaacgaac
     1501 agatacagca tacgtttttg atcccgggag agactatatg ccgcctcagt gaggtcgttt
     1561 gactggacga ttcgcgggct atttttacgt ttcttgtgat tgataaccgc tgtttccgcc
     1621 atgacagatc catgtgaagt gtgacaagtt tttagattgt cacactaaat aaaaaagagt
     1681 caataagcag ggataacttt gtgaaaaaac agcttcttct gagggcaatt tgtcacaggg
     1741 ttaagggcaa tttgtcacag acaggactgt catttgaggg tgatttgtca cactgaaagg
     1801 gcaatttgtc acaacacctt ctgtagaacc agcatggata aaggcctaca aggcgctcta
     1861 aaaaagaaga tctaaaaact ataaaaaaaa taattataaa aatatccccg tggataagtg
     1921 gataacccca agggaagttt tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt
     1981 ccctggtgct tcctcgctca ctcgaaattc ccggggatag ctttatgctt gtaaaccgtt
     2041 ttgtgaaaaa atttttaaaa taaaaaaggg gacctctagg gtccccaatt aattagtaat
     2101 ataatctatt aaaggtcatt caaaaggtca tccaccggat caattcccct gctcgcgcag
     2161 gctgggtgcc aagctctcgg gtaacatcaa ggcccgatcc ttggagccct tgccctcccg
     2221 cacgatgatc gtgccgtgat cgaaatccag atccttgacc cgcagttgca aaccctcact
     2281 gatccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2341 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     2401 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     2461 tatgaccatg attacgccaa gcgcggtacc tgtcgcggca acgcgctaac agacgtagta
     2521 agaaccacca gcattgtaat gctggctaaa gtcactttcc tgagctgtat aacgatgagc
     2581 gattttactt tttctggcta tgaattggcc tgctttgtaa cacactccgg tctatcccgt
     2641 agcgccgggc atatcctgtc gcaatgtgca aatctcgcgg caacaaccag tgaatacttc
     2701 attcacaagc ctcaccgcct gatcgcggca gaaactggtt atagccaatc aaccgtcgtt
     2761 cgtgcattcc gtgaagctgt aaacaaaggt atcctgtctg tagagattgt tatcggcgat
     2821 caccgtgaac gtcgcgctaa cctgtaccgg tttacaccat cctttttggc cttcgcacaa
     2881 caagccaaaa atgcgctgat agaaagcaaa ttaaagatct cttcagcggc aaccaaggtt
     2941 aaagctgttc tcgctaagac attggcttta tttaattttt tatccacacc cccatgtcaa
     3001 aatgataccc cctccccctg tcaggatgac gtggcaataa agaataagaa gtcacaagtt
     3061 aaaaaaacaa aaagatcagt ttccggcggt gccggaacaa ccagcctcaa aaaattgact
     3121 tcatggatcg ctaaggcaaa agcaaaggct gacaatctgc ggttatccaa aaaacgcact
     3181 caaaaacatg agttcaagca gaaagtagag gcggctgcgc ggaaatatgc ttacctgaag
     3241 aacaagcgtt cgcctgatat tggcgggata tcaaacttcg ataacctacc gcattgcatg
     3301 acggtaaacg aagctcttaa tgcggtttta gccaaaaata aagataacga acaatggggt
     3361 ataccggcag gattcagagg gtaatgaatt gctctaatta taaccatgca tactttcaac
     3421 acctctagtt tgccatgagg caaactcata ggtgtcctgg taagaggaca ctgttgccaa
     3481 aactggacgc cccattattg caattaataa acaactaacg gacaattcta cctaacaata
     3541 agtggagttg cggccggccg cgatccgcta gcaaagccac gttgtgtctc aaaatctctg
     3601 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     3661 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgcgccc
     3721 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     3781 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
     3841 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     3901 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     3961 tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata
     4021 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     4081 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca
     4141 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     4201 gcctgttgaa caagtctgga aagaaatgca taaactgttg ccattctcac cggattcagt
     4261 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     4321 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     4381 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     4441 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     4501 agaattggtt aattggttgt aacactggcg agctctgcca cctgacgtct aagaaaccat
     4561 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     4621 aaaatcctta gctttcgcta aggatgattt ctgcacctgc accagtcagt aaaacgacgg
     4681 ccagtagtca aaagcctccg accggaggct tttgacttgg ttcaggtgga gtgggag
//
