LOCUS       Exported                5283 bp DNA     circular SYN 02-JUL-2021
DEFINITION  BBF10K_000513 in pOpen_v4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 2, 2021 from SnapGene 5.3.1
            https://www.snapgene.com
COMMENT     This file was created using tools provided by Twist Bioscience
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="BBF10K_000513 in pOpen_v4"
     primer_bind     21..51
                     /label=Gibson-for
     regulatory      46..55
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             52..789
                     /codon_start=1
                     /product="UL16 binding protein 2"
                     /label=ULBP2
                     /translation="MAAAAATKILLCLPLLLLLSGWSRAGRADPHSLCYDITVIPKFRP
                     GPRWCAVQGQVDEKTFLHYDCGNKTVTPVSPLGKKLNVTTAWKAQNPVLREVVDILTEQ
                     LRDIQLENYTPKEPLTLQARMSCEQKAEGHSSGSWQFSFDGQIFLLFDSEKRMWTTVHP
                     GARKMKEKWENDKVVAMSFHYFSMGDCIGWLEDFLMGMDSTLEPSAGAPLAMSSGTTQL
                     RATATTLILCCLLIILPCFILPGI"
     primer_bind     complement(790..814)
                     /label=Gibson-rev
     terminator      933..980
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(985..1001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1160..1410
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(1413..2168)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      2259..2478
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     protein_bind    2898..2919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2934..2964
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2972..2988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2996..3012
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3085..3930
                     /codon_start=1
                     /label=repL
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCAN
                     LAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANLYR
                     FTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSPCQD
                     DVAIKNKKSQVKKTKRSVSGGAGTTSLKKLTSWIAKAKAKADNLRLSKKRTQKHEFKQK
                     VEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIPAGFRG
                     "
     misc_feature    4038..4189
                     /label=nnpp promoters
     CDS             4229..5044
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      complement(5154..5197)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     5215..5231
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      5232..5263
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aagaattact gacccctcgg cagactgagt cgcccggggg gatccgccac catggccgcc
       61 gccgccgcca ccaagatcct gctgtgcctg cccctgctgc tgctgctgag cggctggagc
      121 agagccggca gagccgaccc ccacagcctg tgctacgaca tcaccgtgat ccccaagttc
      181 agacccggcc ccagatggtg cgccgtgcag ggccaggtgg acgagaagac cttcctgcac
      241 tacgactgcg gcaacaagac cgtgaccccc gtgagccccc tgggcaagaa gctgaacgtg
      301 accaccgcct ggaaggccca gaaccccgtg ctgagagagg tggtggacat cctgaccgag
      361 cagctgagag acatccagct ggagaactac acccccaagg agcccctgac cctgcaggcc
      421 agaatgagct gcgagcagaa ggccgagggc cacagcagcg gcagctggca gttcagcttc
      481 gacggccaga tcttcctgct gttcgacagc gagaagagaa tgtggaccac cgtgcacccc
      541 ggcgccagaa agatgaagga gaagtgggag aacgacaagg tggtggccat gagcttccac
      601 tacttcagca tgggcgactg catcggctgg ctggaggact tcctgatggg catggacagc
      661 accctggagc ccagcgccgg cgcccccctg gccatgagca gcggcaccac ccagctgaga
      721 gccaccgcca ccaccctgat cctgtgctgc ctgctgatca tcctgccctg cttcatcctg
      781 cccggcatcg gatccgccac caacttcagc ctgctagaga ggcatgattg acctcaccgg
      841 cgagtgctga atgtactagg gataacaggg taattctaac cgtttcgttc cgatccgctg
      901 aggtgtcaat cgtcggagcc gctgagcaat aactagcata accccttggg gcctctaaac
      961 gggtcttgag gggttttttg catggtcata gctgtttcct gagagcttgg caggtgtcgc
     1021 gtatgcatcg atcacgtgtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1081 acactcggat cgatccggcg cgcaccgtgg gaaaaactcc aggtagaggt acacacgcgg
     1141 atagccaatt cagagtaata aactgtgata atcaaccctc atcaatgatg acgaactaac
     1201 ccccgatatc aggtcacatg acgaagggaa agagaaggaa atcaactgtg acaaactgcc
     1261 ctcaaatttg gcttccttaa aaattacagt tcaaaaagta tgagaaaatc catgcaggct
     1321 gaaggaaaca gcaaaactgt gacaaattac cctcagtagg tcagaacaaa tgtgacgaac
     1381 caccctcaaa tctgtgacag ataaccctca gactatcctg tcgtcatgga agtgatatcg
     1441 cggaaggaaa atacgatatg agtcgtctgg cggcctttct ttttctcaat gtatgagagg
     1501 cgcattggag ttctgctgtt gatctcatta acacagactt gcaggaagcg gcggcggaag
     1561 tcaggcatac gctggtaact ttgaggcagc tggtaacgct ctatgatcca gtcgattttc
     1621 agagacacga tgcctgagcc atccggctta cgatactgac acagggattc gtataaacgc
     1681 atggcatacg gattggtgat ttcttttgtt tcactaagcc gaaactgcgt aaaccggttc
     1741 tgtaacccga taaagaaggg aatgagatat gggttgatat gtacactgta aagccctctg
     1801 gatggactgt gcgcacgttt gataaaccaa ggaaaagatt catagccttt ttcatcaccg
     1861 gcatcctctt cagggcgata aaaaaccact tccttccccg cgaaactctt caatgcctgc
     1921 cgtatatcct tactggcttc cgcagaggtc aatccgaata tttcagcata tttagcaaca
     1981 tggatctcgc agataccgtc atgttcctgt agggtgccat cagattttct gatctggtca
     2041 acgaacagat acagcatacg tttttgatcc cgggagagac tatatgccgc ctcagtgagg
     2101 tcgtttgact ggacgattcg cgggctattt ttacgtttct tgtgattgat aaccgctgtt
     2161 tccgccatga cagatccatg tgaagtgtga caagttttta gattgtcaca ctaaataaaa
     2221 aagagtcaat aagcagggat aactttgtga aaaaacagct tcttctgagg gcaatttgtc
     2281 acagggttaa gggcaatttg tcacagacag gactgtcatt tgagggtgat ttgtcacact
     2341 gaaagggcaa tttgtcacaa caccttctgt agaaccagca tggataaagg cctacaaggc
     2401 gctctaaaaa agaagatcta aaaactataa aaaaaataat tataaaaata tccccgtgga
     2461 taagtggata accccaaggg aagttttttc aggcatcgtg tgtaagcaga atatataagt
     2521 gctgttccct ggtgcttcct cgctcactcg aaattcccgg ggatagcttt atgcttgtaa
     2581 accgttttgt gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc ccaattaatt
     2641 agtaatataa tctattaaag gtcattcaaa aggtcatcca ccggatcaat tcccctgctc
     2701 gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc cgatccttgg agcccttgcc
     2761 ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc
     2821 ctcactgatc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2881 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     2941 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     3001 aacagctatg accatgatta cgccaagcgc ggtacctgtc gcggcaacgc gctaacagac
     3061 gtagtaagaa ccaccagcat tgtaatgctg gctaaagtca ctttcctgag ctgtataacg
     3121 atgagcgatt ttactttttc tggctatgaa ttggcctgct ttgtaacaca ctccggtcta
     3181 tcccgtagcg ccgggcatat cctgtcgcaa tgtgcaaatc tcgcggcaac aaccagtgaa
     3241 tacttcattc acaagcctca ccgcctgatc gcggcagaaa ctggttatag ccaatcaacc
     3301 gtcgttcgtg cattccgtga agctgtaaac aaaggtatcc tgtctgtaga gattgttatc
     3361 ggcgatcacc gtgaacgtcg cgctaacctg taccggttta caccatcctt tttggccttc
     3421 gcacaacaag ccaaaaatgc gctgatagaa agcaaattaa agatctcttc agcggcaacc
     3481 aaggttaaag ctgttctcgc taagacattg gctttattta attttttatc cacaccccca
     3541 tgtcaaaatg ataccccctc cccctgtcag gatgacgtgg caataaagaa taagaagtca
     3601 caagttaaaa aaacaaaaag atcagtttcc ggcggtgccg gaacaaccag cctcaaaaaa
     3661 ttgacttcat ggatcgctaa ggcaaaagca aaggctgaca atctgcggtt atccaaaaaa
     3721 cgcactcaaa aacatgagtt caagcagaaa gtagaggcgg ctgcgcggaa atatgcttac
     3781 ctgaagaaca agcgttcgcc tgatattggc gggatatcaa acttcgataa cctaccgcat
     3841 tgcatgacgg taaacgaagc tcttaatgcg gttttagcca aaaataaaga taacgaacaa
     3901 tggggtatac cggcaggatt cagagggtaa tgaattgctc taattataac catgcatact
     3961 ttcaacacct ctagtttgcc atgaggcaaa ctcataggtg tcctggtaag aggacactgt
     4021 tgccaaaact ggacgcccca ttattgcaat taataaacaa ctaacggaca attctaccta
     4081 acaataagtg gagttgcggc cggccgcgat ccgctagcaa agccacgttg tgtctcaaaa
     4141 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     4201 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc
     4261 gcgcccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     4321 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
     4381 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     4441 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     4501 tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga
     4561 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     4621 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gcctcgctca
     4681 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     4741 tggctggcct gttgaacaag tctggaaaga aatgcataaa ctgttgccat tctcaccgga
     4801 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     4861 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     4921 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     4981 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     5041 ctaatcagaa ttggttaatt ggttgtaaca ctggcgagct ctgccacctg acgtctaaga
     5101 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
     5161 ataaaaaaaa tccttagctt tcgctaagga tgatttctgc acctgcacca gtcagtaaaa
     5221 cgacggccag tagtcaaaag cctccgaccg gaggcttttg acttggttca ggtggagtgg
     5281 gag
//
