LOCUS Copy_of_Inorganic_Pyrop 5797 bp ds-DNA circular 23-MAR-2021 DEFINITION . FEATURES Location/Qualifiers origin 342..961 /label="pBR322 Origin" /ApEinfo_revcolor=#9faa9a /ApEinfo_fwdcolor=#9faa9a gene 1067..1882 /label="Kan R2" /ApEinfo_revcolor=#359797 /ApEinfo_fwdcolor=#359797 terminator 2442..2570 /label="T7 Terminator" /ApEinfo_revcolor=#d24b19 /ApEinfo_fwdcolor=#d24b19 primer 2443..2467 /label="pET28+ Forward Primer" /ApEinfo_revcolor=#fffafa /ApEinfo_fwdcolor=#fffafa gene 2578..3108 /label="Inorganic Pyrophosphotase" /ApEinfo_revcolor=#0000ff /ApEinfo_fwdcolor=#0000ff tag 3112..3129 /label="thrombin" /ApEinfo_revcolor=#5f631d /ApEinfo_fwdcolor=#5f631d tag 3139..3156 /label="His Tag" /ApEinfo_revcolor=#f9c5fe /ApEinfo_fwdcolor=#f9c5fe feature 3176..3192 /label="T7 Ribosome Binding Site" /ApEinfo_revcolor=#e12222 /ApEinfo_fwdcolor=#e12222 regulatory 3210..3237 /label="lacO" /ApEinfo_revcolor=#fffbaa /ApEinfo_fwdcolor=#fffbaa regulatory 3210..3237 /label="lacI" /ApEinfo_revcolor=#c3dff3 /ApEinfo_fwdcolor=#c3dff3 promoter 3237..3255 /label="T7 Promoter" /ApEinfo_revcolor=#c8498b /ApEinfo_fwdcolor=#c8498b gene 3287..3550 /label="tet (300-563)" /ApEinfo_revcolor=#ffe2b9 /ApEinfo_fwdcolor=#ffe2b9 primer 3338..3358 /label="pET28+ Reverse Primer" /ApEinfo_revcolor=#fffafa /ApEinfo_fwdcolor=#fffafa feature 4783..5058 /label="tet(576-851)" /ApEinfo_revcolor=#ba9231 /ApEinfo_fwdcolor=#ba9231 feature 5533..5724 /label="ROP" /ApEinfo_revcolor=#1ebdac /ApEinfo_fwdcolor=#1ebdac primer 5718..5740 /label="pGEX_3_primer" /ApEinfo_revcolor=#5b637e /ApEinfo_fwdcolor=#5b637e ORIGIN 1 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG 61 GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT 121 ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC 181 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT 241 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 301 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC 361 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 421 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT 481 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG 541 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 601 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT 661 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 721 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC 781 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC 841 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 901 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC 961 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 1021 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA 1081 ACGGGAAACG TCTTGCTCTA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 1141 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG 1201 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 1261 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 1321 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGGAAAAC 1381 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 1441 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 1501 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 1561 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 1621 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 1681 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 1741 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 1801 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 1861 GATGCTCGAT GAGTTTTTCT AAGAATTAAT TCATGAGCGG ATACATATTT GAATGTATTT 1921 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAAATTG 1981 TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA 2041 ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT 2101 TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA 2161 AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA 2221 GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT 2281 TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG 2341 GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG 2401 CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CCCATTCGCC AATCCGGATA TAGTTCCTCC 2461 TTTCAGCAAA AAACCCCTCA AGACCCGTTT AGAGGCCCCA AGGGGTTATG CTAGTTATTG 2521 CTCAGCGGTG GCAGCAGCCA ACTCAGCTTC CTTTCGGGCT TTGTTAGCAG CCGGATCTTA 2581 TTTATTCTTT GCGCGCTCGA AGGAGGCAAC GATTTCAGCT TTAGCGGCTT CTGCGTTTTC 2641 CCAACCTTCA ACTTTCACCC ACTTGCCTTT TTCGAGGTCT TTGTAGTGCT CGAAGAAGTG 2701 AGCGATTTGC GCTTTCAGCA GTTCAGGCAG ATCGTTAACG TCTTTAATGT GATCGTATTC 2761 TTTGCTCAGC TTGCTGTGCG GAACCGCAAC CAGTTTCGCA TCTTCACCGG CTTCGTCGGT 2821 CATTTTCAGA ACGCCAACCG GACGGCAACG GATCACAGAA CCCGGCTGCA GCGGGTACGG 2881 AGTTGGGACC AGTACGTCAA CCGGGTCACC GTCCAGAGAC AGGGTGTGGT TGATGTAACC 2941 GTAGTTGCAT GGATAGAACA TCGCGGTGGA CATGAAGCGG TCAACGAACA GTGCGCCGCT 3001 CTCTTTGTCG ATTTCGTATT TGATCGGATC TGCGTTAGCC GGGATCTCAA TAACAACGTA 3061 GATGTCTTCC GGCAGATCTT TACCCGCAGG GACGTTGAGT AAGCTCATAT GGCTGCCGCG 3121 CGGCACCAGG CCGCTGCTGT GATGATGATG ATGATGGCTG CTGCCCATGG TATATCTCCT 3181 TCTTAAAGTT AAACAAAATT ATTTCTAGAG GGGAATTGTT ATCCGCTCAC AATTCCCCTA 3241 TAGTGAGTCG TATTAATTTC GCGGGATCGA GATCTCGATC CTCTACGCCG GACGCATCGT 3301 GGCCGGCATC ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA 3361 TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT 3421 GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC 3481 GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA 3541 TAAGGGAGAG CGTCGAGATC CCGGACACCA TCGAATGGCG CAAAACCTTT CGCGGTATGG 3601 CATGATAGCG CCCGGAAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT 3661 ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG 3721 CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT 3781 ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG 3841 CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG 3901 CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT 3961 GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC ATTAACTATC 4021 CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT 4081 TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT GAAGACGGTA 4141 CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA GCAAATCGCG CTGTTAGCGG 4201 GCCCATTAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG CTGGCATAAA TATCTCACTC 4261 GCAATCAAAT TCAGCCGATA GCGGAACGGG AAGGCGACTG GAGTGCCATG TCCGGTTTTC 4321 AACAAACCAT GCAAATGCTG AATGAGGGCA TCGTTCCCAC TGCGATGCTG GTTGCCAACG 4381 ATCAGATGGC GCTGGGCGCA ATGCGCGCCA TTACCGAGTC CGGGCTGCGC GTTGGTGCGG 4441 ATATCTCGGT AGTGGGATAC GACGATACCG AAGACAGCTC ATGTTATATC CCGCCGTTAA 4501 CCACCATCAA ACAGGATTTT CGCCTGCTGG GGCAAACCAG CGTGGACCGC TTGCTGCAAC 4561 TCTCTCAGGG CCAGGCGGTG AAGGGCAATC AGCTGTTGCC CGTCTCACTG GTGAAAAGAA 4621 AAACCACCCT GGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 4681 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT 4741 GTAAGTTAGC TCACTCATTA GGCACCGGGA TCTCGACCGA TGCCCTTGAG AGCCTTCAAC 4801 CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC 4861 TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTGGGTCAT TTTCGGCGAG 4921 GACCGCTTTC GCTGGAGCGC GACGATGATC GGCCTGTCGC TTGCGGTATT CGGAATCTTG 4981 CACGCCCTCG CTCAAGCCTT CGTCACTGGT CCCGCCACCA AACGTTTCGG CGAGAAGCAG 5041 GCCATTATCG CCGGCATGGC GGCCCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG 5101 ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG 5161 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC ATGAATGGTC 5221 TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT CAGCGCCCTG CACCATTATG 5281 TTCCGGATCT GCATCGCAGG ATGCTGCTGG CTACCCTGTG GAACACCTAC ATCTGTATTA 5341 ACGAAGCGCT GGCATTGACC CTGAGTGATT TTTCTCTGGT CCCGCCGCAT CCATACCGCC 5401 AGTTGTTTAC CCTCACAACG TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC 5461 GTGAGCATCC TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC 5521 ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG CTTTATCAGA 5581 AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG ACGCGGATGA ACAGGCAGAC 5641 ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC GCAGCTGCCT CGCGCGTTTC 5701 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG 5761 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCG //