LOCUS       Copy_of_Inorganic_Pyrop 5797 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     origin          342..961
                     /label="pBR322 Origin"
                     /ApEinfo_revcolor=#9faa9a
                     /ApEinfo_fwdcolor=#9faa9a
     gene            1067..1882
                     /label="Kan R2"
                     /ApEinfo_revcolor=#359797
                     /ApEinfo_fwdcolor=#359797
     terminator      2442..2570
                     /label="T7 Terminator"
                     /ApEinfo_revcolor=#d24b19
                     /ApEinfo_fwdcolor=#d24b19
     primer          2443..2467
                     /label="pET28+ Forward Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     gene            2578..3108
                     /label="Inorganic Pyrophosphotase"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     tag             3112..3129
                     /label="thrombin"
                     /ApEinfo_revcolor=#5f631d
                     /ApEinfo_fwdcolor=#5f631d
     tag             3139..3156
                     /label="His Tag"
                     /ApEinfo_revcolor=#f9c5fe
                     /ApEinfo_fwdcolor=#f9c5fe
     feature         3176..3192
                     /label="T7 Ribosome Binding Site"
                     /ApEinfo_revcolor=#e12222
                     /ApEinfo_fwdcolor=#e12222
     regulatory      3210..3237
                     /label="lacO"
                     /ApEinfo_revcolor=#fffbaa
                     /ApEinfo_fwdcolor=#fffbaa
     regulatory      3210..3237
                     /label="lacI"
                     /ApEinfo_revcolor=#c3dff3
                     /ApEinfo_fwdcolor=#c3dff3
     promoter        3237..3255
                     /label="T7 Promoter"
                     /ApEinfo_revcolor=#c8498b
                     /ApEinfo_fwdcolor=#c8498b
     gene            3287..3550
                     /label="tet (300-563)"
                     /ApEinfo_revcolor=#ffe2b9
                     /ApEinfo_fwdcolor=#ffe2b9
     primer          3338..3358
                     /label="pET28+ Reverse Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     feature         4783..5058
                     /label="tet(576-851)"
                     /ApEinfo_revcolor=#ba9231
                     /ApEinfo_fwdcolor=#ba9231
     feature         5533..5724
                     /label="ROP"
                     /ApEinfo_revcolor=#1ebdac
                     /ApEinfo_fwdcolor=#1ebdac
     primer          5718..5740
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#5b637e
                     /ApEinfo_fwdcolor=#5b637e
ORIGIN
        1 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG
       61 GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT
      121 ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC
      181 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
      241 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
      301 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
      361 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA
      421 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
      481 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
      541 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
      601 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
      661 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
      721 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
      781 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC
      841 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT
      901 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC
      961 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     1021 AACAATAAAA CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA
     1081 ACGGGAAACG TCTTGCTCTA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG
     1141 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG
     1201 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT
     1261 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA
     1321 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGGAAAAC
     1381 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC
     1441 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG
     1501 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA
     1561 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT
     1621 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT
     1681 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG
     1741 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA
     1801 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT
     1861 GATGCTCGAT GAGTTTTTCT AAGAATTAAT TCATGAGCGG ATACATATTT GAATGTATTT
     1921 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAAATTG
     1981 TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA
     2041 ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT
     2101 TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA
     2161 AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA
     2221 GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT
     2281 TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG
     2341 GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG
     2401 CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CCCATTCGCC AATCCGGATA TAGTTCCTCC
     2461 TTTCAGCAAA AAACCCCTCA AGACCCGTTT AGAGGCCCCA AGGGGTTATG CTAGTTATTG
     2521 CTCAGCGGTG GCAGCAGCCA ACTCAGCTTC CTTTCGGGCT TTGTTAGCAG CCGGATCTTA
     2581 TTTATTCTTT GCGCGCTCGA AGGAGGCAAC GATTTCAGCT TTAGCGGCTT CTGCGTTTTC
     2641 CCAACCTTCA ACTTTCACCC ACTTGCCTTT TTCGAGGTCT TTGTAGTGCT CGAAGAAGTG
     2701 AGCGATTTGC GCTTTCAGCA GTTCAGGCAG ATCGTTAACG TCTTTAATGT GATCGTATTC
     2761 TTTGCTCAGC TTGCTGTGCG GAACCGCAAC CAGTTTCGCA TCTTCACCGG CTTCGTCGGT
     2821 CATTTTCAGA ACGCCAACCG GACGGCAACG GATCACAGAA CCCGGCTGCA GCGGGTACGG
     2881 AGTTGGGACC AGTACGTCAA CCGGGTCACC GTCCAGAGAC AGGGTGTGGT TGATGTAACC
     2941 GTAGTTGCAT GGATAGAACA TCGCGGTGGA CATGAAGCGG TCAACGAACA GTGCGCCGCT
     3001 CTCTTTGTCG ATTTCGTATT TGATCGGATC TGCGTTAGCC GGGATCTCAA TAACAACGTA
     3061 GATGTCTTCC GGCAGATCTT TACCCGCAGG GACGTTGAGT AAGCTCATAT GGCTGCCGCG
     3121 CGGCACCAGG CCGCTGCTGT GATGATGATG ATGATGGCTG CTGCCCATGG TATATCTCCT
     3181 TCTTAAAGTT AAACAAAATT ATTTCTAGAG GGGAATTGTT ATCCGCTCAC AATTCCCCTA
     3241 TAGTGAGTCG TATTAATTTC GCGGGATCGA GATCTCGATC CTCTACGCCG GACGCATCGT
     3301 GGCCGGCATC ACCGGCGCCA CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA
     3361 TGGGGAAGAT CGGGCTCGCC ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT
     3421 GGCAGGCCCC GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC
     3481 GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA
     3541 TAAGGGAGAG CGTCGAGATC CCGGACACCA TCGAATGGCG CAAAACCTTT CGCGGTATGG
     3601 CATGATAGCG CCCGGAAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT
     3661 ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG
     3721 CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT
     3781 ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG
     3841 CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG
     3901 CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC GTCGAAGCCT
     3961 GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC ATTAACTATC
     4021 CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT CCGGCGTTAT
     4081 TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT GAAGACGGTA
     4141 CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA GCAAATCGCG CTGTTAGCGG
     4201 GCCCATTAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG CTGGCATAAA TATCTCACTC
     4261 GCAATCAAAT TCAGCCGATA GCGGAACGGG AAGGCGACTG GAGTGCCATG TCCGGTTTTC
     4321 AACAAACCAT GCAAATGCTG AATGAGGGCA TCGTTCCCAC TGCGATGCTG GTTGCCAACG
     4381 ATCAGATGGC GCTGGGCGCA ATGCGCGCCA TTACCGAGTC CGGGCTGCGC GTTGGTGCGG
     4441 ATATCTCGGT AGTGGGATAC GACGATACCG AAGACAGCTC ATGTTATATC CCGCCGTTAA
     4501 CCACCATCAA ACAGGATTTT CGCCTGCTGG GGCAAACCAG CGTGGACCGC TTGCTGCAAC
     4561 TCTCTCAGGG CCAGGCGGTG AAGGGCAATC AGCTGTTGCC CGTCTCACTG GTGAAAAGAA
     4621 AAACCACCCT GGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA
     4681 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT
     4741 GTAAGTTAGC TCACTCATTA GGCACCGGGA TCTCGACCGA TGCCCTTGAG AGCCTTCAAC
     4801 CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC
     4861 TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTGGGTCAT TTTCGGCGAG
     4921 GACCGCTTTC GCTGGAGCGC GACGATGATC GGCCTGTCGC TTGCGGTATT CGGAATCTTG
     4981 CACGCCCTCG CTCAAGCCTT CGTCACTGGT CCCGCCACCA AACGTTTCGG CGAGAAGCAG
     5041 GCCATTATCG CCGGCATGGC GGCCCCACGG GTGCGCATGA TCGTGCTCCT GTCGTTGAGG
     5101 ACCCGGCTAG GCTGGCGGGG TTGCCTTACT GGTTAGCAGA ATGAATCACC GATACGCGAG
     5161 CGAACGTGAA GCGACTGCTG CTGCAAAACG TCTGCGACCT GAGCAACAAC ATGAATGGTC
     5221 TTCGGTTTCC GTGTTTCGTA AAGTCTGGAA ACGCGGAAGT CAGCGCCCTG CACCATTATG
     5281 TTCCGGATCT GCATCGCAGG ATGCTGCTGG CTACCCTGTG GAACACCTAC ATCTGTATTA
     5341 ACGAAGCGCT GGCATTGACC CTGAGTGATT TTTCTCTGGT CCCGCCGCAT CCATACCGCC
     5401 AGTTGTTTAC CCTCACAACG TTCCAGTAAC CGGGCATGTT CATCATCAGT AACCCGTATC
     5461 GTGAGCATCC TCTCTCGTTT CATCGGTATC ATTACCCCCA TGAACAGAAA TCCCCCTTAC
     5521 ACGGAGGCAT CAGTGACCAA ACAGGAAAAA ACCGCCCTTA ACATGGCCCG CTTTATCAGA
     5581 AGCCAGACAT TAACGCTTCT GGAGAAACTC AACGAGCTGG ACGCGGATGA ACAGGCAGAC
     5641 ATCTGTGAAT CGCTTCACGA CCACGCTGAT GAGCTTTACC GCAGCTGCCT CGCGCGTTTC
     5701 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG
     5761 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCG
//