LOCUS       Copy_of_Methionine-tRNA 7258 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     origin          344..963
                     /label="pBR322 Origin"
                     /ApEinfo_revcolor=#9faa9a
                     /ApEinfo_fwdcolor=#9faa9a
     gene            1069..1884
                     /label="Kan R2"
                     /ApEinfo_revcolor=#359797
                     /ApEinfo_fwdcolor=#359797
     terminator      2444..2572
                     /label="T7 Terminator"
                     /ApEinfo_revcolor=#d24b19
                     /ApEinfo_fwdcolor=#d24b19
     primer          2445..2469
                     /label="pET28+ Forward Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     feature         2580..2600
                     /label="His Tag and Stop Codon"
                     /ApEinfo_revcolor=#ddb34e
                     /ApEinfo_fwdcolor=#ddb34e
     gene            2601..4631
                     /label="Methionine-tRNA ligase"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     primer          2970..2997
                     /label="seqFOR1 Methionine-tRNA ligase C-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     primer          3497..3528
                     /label="seqFOR2 Methionine-tRNA ligase C-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     primer          4029..4055
                     /label="seqFOR3 Methionine-tRNA ligase C-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     feature         4639..4655
                     /label="T7 Ribosome Binding Site"
                     /ApEinfo_revcolor=#e12222
                     /ApEinfo_fwdcolor=#e12222
     regulatory      4673..4700
                     /label="lacI"
                     /ApEinfo_revcolor=#c3dff3
                     /ApEinfo_fwdcolor=#c3dff3
     regulatory      4673..4700
                     /label="lacO"
                     /ApEinfo_revcolor=#fffbaa
                     /ApEinfo_fwdcolor=#fffbaa
     promoter        4700..4718
                     /label="T7 Promoter"
                     /ApEinfo_revcolor=#c8498b
                     /ApEinfo_fwdcolor=#c8498b
     gene            4750..5013
                     /label="tet (300-563)"
                     /ApEinfo_revcolor=#ffe2b9
                     /ApEinfo_fwdcolor=#ffe2b9
     primer          4801..4821
                     /label="pET28+ Reverse Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     feature         6246..6521
                     /label="tet(576-851)"
                     /ApEinfo_revcolor=#ba9231
                     /ApEinfo_fwdcolor=#ba9231
     feature         6996..7187
                     /label="ROP"
                     /ApEinfo_revcolor=#1ebdac
                     /ApEinfo_fwdcolor=#1ebdac
     primer          7181..7203
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#5b637e
                     /ApEinfo_fwdcolor=#5b637e
ORIGIN
        1 CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG
       61 CGGAGTGTAT ACTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT
      121 ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT
      181 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG
      241 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA
      301 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT
      361 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC
      421 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
      481 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
      541 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
      601 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT
      661 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA
      721 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
      781 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
      841 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
      901 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA
      961 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA
     1021 TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT
     1081 CAACGGGAAA CGTCTTGCTC TAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT
     1141 GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA TCGATTGTAT
     1201 GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT
     1261 GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC
     1321 AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGGAAA
     1381 ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG
     1441 GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT
     1501 CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT
     1561 GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA
     1621 CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT
     1681 ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC
     1741 CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG
     1801 AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT
     1861 TTGATGCTCG ATGAGTTTTT CTAAGAATTA ATTCATGAGC GGATACATAT TTGAATGTAT
     1921 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGAAAT
     1981 TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT
     2041 TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG
     2101 GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT
     2161 CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC
     2221 AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG
     2281 ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA
     2341 AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC
     2401 CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCCATTCG CCAATCCGGA TATAGTTCCT
     2461 CCTTTCAGCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC CAAGGGGTTA TGCTAGTTAT
     2521 TGCTCAGCGG TGGCAGCAGC CAACTCAGCT TCCTTTCGGG CTTTGTTAGC AGCCGGATCT
     2581 CAGTGGTGGT GGTGGTGGTG TTTCACCTGA TGACCCGGTT TAGCACCGGC ATCCGGGCTT
     2641 AGCAGGAAAA TATCTTTCCC GCCAGGACCG GCAGCCATCA CCATGCCTTC AGAGATACCG
     2701 AAGCGCATTT TACGTGGTGC CAGGTTAGCC ACCATAATGG TGTGACGACC AATCAGTGCC
     2761 TGCGGATCCG GGTAAGCAGA ACGAATACCG GAGAAGACAT TGCGTTTTTC ACCGCCGAGA
     2821 TCCAGCGTCA GGCGCAGCAG TTTGTCAGAA CCTTCAACAA ACTCTGCGTT TTCAATCAGC
     2881 GCCACGCGCA GGTCAACTTT AGCGAAGTCG TCAAAGGTGA TGGTTTCCTG AATCGGATCA
     2941 TCTGCCAGCG GGCCAGTTAC CGGCGCGGCA GCGGCTTTTA CTTCTTCTTT AGAGGCTTCC
     3001 ACCAGTGCTT CAACCTGCCT CATATCGATG CGGTTATACA GCGCCTTGAA CGGATTCACT
     3061 TTGTGGCCCA GCAGCGGTTG CTGGATACCA TCCCAGGTCA GTTCCGTATT GAGGAATGCT
     3121 TCTGCACGCT CGGTCAGTTT CGGCAGTACC GGCTTCAGGT AAGTCATCAG CACGCGGAAC
     3181 AGGTTGATGC CCATTGAGCA AATTGCCTGC AGGTCGGCAT CGCGGCCTTC CTGTTTCGCC
     3241 ACCACCCACG GAGCCTGTTC ATCGACATAG CGGTTAGCCA GATCAGCCAG CGCCATGATT
     3301 TCGCGCACGG CTTTACCAAA TTCACGGCTT TCCCACGCTT CACCAATCAC TTCAGCGGCA
     3361 TCAGTGAAGG TTTTGTACAA CTGCGGGTCA GCCAGTTCGC TTGCCAGCAC GCCGTCAAAA
     3421 CGCTTGTTGA TAAAGCCCGC ATTACGGGAG GCCAGGTTAA CCACTTTGTT AACGATATCG
     3481 GCATTCACAC GCTGAACGAA ATCTTCCAGG TTGAGATCGA TATCATCAAT GCGCGAAGAG
     3541 AGTTTCGCAG TGTAGTAGTA ACGCAGGCTG TCTGCGTCAA AATGATTCAG CCAGGTGCTG
     3601 GCTTTAATAA AGGTGCCGCG AGACTTGGAC ATCTTTGCGC CGTTCACCGT CACATAGCCA
     3661 TGAACAAACA GGTTGGACGG CTTGCGGAAG TTGCTGCCTT CCAGCATGGC AGGCCAGAAC
     3721 AGGCTGTGGA AGTAAACAAT ATCTTTACCG ATGAAGTGGT ACAGCTCGGC GGTGGAGTCT
     3781 TTCTTCCAGT ATTCATCGAA GCTTACGCTG TCGCCGCGCT TGTCGCACAG ATTCTTGAAA
     3841 GAACCCATGT AGCCAATCGG TGCGTCCAGC CAGACGTAGA AATATTTGCC CGGCGCGTTC
     3901 GGAATTTCAA AACCGAAGTA AGGGGCGTCG CGGGAGATAT CCCACTGTTG CAGGCCAGAT
     3961 TCAAACCACT CCTGCATTTT ATTTGCCACC TGCTCCTGCA ACGCACCGCT GCGGGTCCAT
     4021 GCCTGCAACA TTTCGCTGAA AGAGGGCAGA TCAAAGAAGA AGTGTTCAGA ATCACGCATT
     4081 ACCGGCGTAG CGCCAGAAAC CACCGATTTC GGCTCGATCA GTTCAGTCGG GCTGTAGGTC
     4141 GCGCCGCAGA CTTCGCAGTT ATCGCCGTAT TGATCCGGGG ATTTACATTT CGGGCAGGTG
     4201 CCTTTCACAA AACGGTCCGG CAGGAACATG CCTTTTTCCG GATCGTACAG CTGAGAGATG
     4261 GTGCGGTTTT TAATAAAACC GTTTTCTTTC AGGCGAGAGT AGATAAGTTC TGACAACTGG
     4321 CGGTTCTCTT CGCTGTGCGT CGAGTGATAG TTGTCATAGC TGATGTTAAA GCCTGCGAAA
     4381 TCAGTCTGAT GCTCCTGACT CATTTCGCCA ATCATCTGCT CCGGGGTGAT ACCAAGCTGC
     4441 TGAGCTTTCA GCATGATCGG TGTACCGTGG GCATCGTCGG CGCAGATGAA GTTGACCTCG
     4501 TGGCCGCGCA TTCGCTGGTA ACGGACCCAG ACATCAGCCT GGATGTGCTC CAGCATATGG
     4561 CCGAGGTGGA TTGAGCCGTT AGCGTACGGC AGTGCGCACG TCACCAGAAT TTTCTTCGCG
     4621 ACTTGAGTCA TGGTATATCT CCTTCTTAAA GTTAAACAAA ATTATTTCTA GAGGGGAATT
     4681 GTTATCCGCT CACAATTCCC CTATAGTGAG TCGTATTAAT TTCGCGGGAT CGAGATCTCG
     4741 ATCCTCTACG CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC
     4801 GCCTATATCG CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC
     4861 GCTTGTTTCG GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC
     4921 TCCTTGCATG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC
     4981 TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAG ATCCCGGACA CCATCGAATG
     5041 GCGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT
     5101 GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC
     5161 CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA
     5221 AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA
     5281 ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT
     5341 TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT
     5401 AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT
     5461 CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC
     5521 CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT
     5581 TATTTTCTCC CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA
     5641 CCAGCAAATC GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC
     5701 TGGCTGGCAT AAATATCTCA CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA
     5761 CTGGAGTGCC ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC
     5821 CACTGCGATG CTGGTTGCCA ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA
     5881 GTCCGGGCTG CGCGTTGGTG CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG
     5941 CTCATGTTAT ATCCCGCCGT TAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC
     6001 CAGCGTGGAC CGCTTGCTGC AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT
     6061 GCCCGTCTCA CTGGTGAAAA GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC
     6121 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG
     6181 GCAGTGAGCG CAACGCAATT AATGTAAGTT AGCTCACTCA TTAGGCACCG GGATCTCGAC
     6241 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA
     6301 TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG
     6361 CGCTCTGGGT CATTTTCGGC GAGGACCGCT TTCGCTGGAG CGCGACGATG ATCGGCCTGT
     6421 CGCTTGCGGT ATTCGGAATC TTGCACGCCC TCGCTCAAGC CTTCGTCACT GGTCCCGCCA
     6481 CCAAACGTTT CGGCGAGAAG CAGGCCATTA TCGCCGGCAT GGCGGCCCCA CGGGTGCGCA
     6541 TGATCGTGCT CCTGTCGTTG AGGACCCGGC TAGGCTGGCG GGGTTGCCTT ACTGGTTAGC
     6601 AGAATGAATC ACCGATACGC GAGCGAACGT GAAGCGACTG CTGCTGCAAA ACGTCTGCGA
     6661 CCTGAGCAAC AACATGAATG GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG GAAACGCGGA
     6721 AGTCAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC AGGATGCTGC TGGCTACCCT
     6781 GTGGAACACC TACATCTGTA TTAACGAAGC GCTGGCATTG ACCCTGAGTG ATTTTTCTCT
     6841 GGTCCCGCCG CATCCATACC GCCAGTTGTT TACCCTCACA ACGTTCCAGT AACCGGGCAT
     6901 GTTCATCATC AGTAACCCGT ATCGTGAGCA TCCTCTCTCG TTTCATCGGT ATCATTACCC
     6961 CCATGAACAG AAATCCCCCT TACACGGAGG CATCAGTGAC CAAACAGGAA AAAACCGCCC
     7021 TTAACATGGC CCGCTTTATC AGAAGCCAGA CATTAACGCT TCTGGAGAAA CTCAACGAGC
     7081 TGGACGCGGA TGAACAGGCA GACATCTGTG AATCGCTTCA CGACCACGCT GATGAGCTTT
     7141 ACCGCAGCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC
     7201 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGG
//