LOCUS       pET-28a_(+)_ArgRS       7000 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
KEYWORDS    "accession:addgene_vdb_2565"                                       
FEATURES             Location/Qualifiers
     primer          808..830
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         complement(824..1015)
                     /label="ROP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            complement(1490..1765)
                     /label="tet (576 - 851)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             1824..2915
                     /label="Translation 1824-2915"
     regulatory      complement(1824..2915)
                     /label="lacI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            complement(2998..3261)
                     /label="tet (300 - 563)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3190..3209
                     /label="pBRrevBam_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3293..3311
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     regulatory      3311..3338
                     /label="lacO"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     feature         3356..3372
                     /label="T7_transl_en_RBS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3364..3388)
                     /label="Tentative pET28 anneal FOR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3380..3382
                     /label="Start Codon"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3380..5187
                     /label="Translation 3380-5187"
     misc_feature    3392..3409
                     /label="His Tag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             3392..5173
                     /label="Translation 3392-5173"
     tag             3419..3436
                     /label="Thrombin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3439..3439
                     /label="Xpress_fwd_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(3439..3439)
                     /label="Tim Excises"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            3440..5173
                     /label="argS gene"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             3440..5173
                     /label="argS CDS (arginyl-tRNA synthetase)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             3440..5173
                     /label="Translation 3440-5173"
     terminator      5181..5309
                     /label="T7_terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5185..5187
                     /label="Stop Codon"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(5223..5241)
                     /label="T7_Terminal_primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            complement(5869..6684)
                     /label="KanR2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     origin          join(6790..7000,1..409)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
       61 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      421 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      481 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
      541 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
      601 tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct
      661 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
      721 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      781 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
      841 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
      901 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
      961 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     1021 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     1081 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     1141 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     1201 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     1261 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     1321 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     1381 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     1441 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga
     1501 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     1561 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     1621 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     1681 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     1741 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct
     1801 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1861 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
     1921 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
     1981 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     2041 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
     2101 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     2161 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
     2221 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
     2281 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
     2341 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
     2401 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
     2461 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
     2521 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
     2581 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
     2641 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
     2701 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
     2761 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     2821 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     2881 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     2941 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     3001 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     3061 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     3121 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     3181 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     3241 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
     3301 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
     3361 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
     3421 ggtgccgcgc ggcagccata tgaatattca ggctcttctc tcagaaaaag tccgtcaggc
     3481 catgattgcg gcaggcgcgc ctgcggattg cgaaccgcag gttcgtcagt cagcaaaagt
     3541 tcagttcggc gactatcagg ctaacggcat gatggcagtt gctaaaaaac tgggtatggc
     3601 accgcgacaa ttagcagagc aggtgctgac tcatctggat cttaacggta tcgccagcaa
     3661 agttgagatc gccggtccag gctttatcaa cattttcctt gatccggcat tcctggctga
     3721 acatgttcag caggcgctgg cgtccgatcg tctcggtgtt gctacgccag aaaaacagac
     3781 cattgtggtt gactactctg cgccaaacgt ggcgaaagag atgcatgtcg gtcacctgcg
     3841 ctctaccatt attggtgacg cagcagtgcg tactctggag ttcctcggtc acaaagtgat
     3901 tcgcgcaaac cacgtcggcg actggggcac tcagttcggt atgctgattg catggctgga
     3961 aaagcagcag caggaaaacg ccggtgaaat ggagctggct gaccttgaag gtttctaccg
     4021 cgatgcgaaa aagcattacg atgaagatga agagttcgcc gagcgcgcac gtaactacgt
     4081 ggtaaaactg caaagcggtg acgaatattt ccgcgagatg tggcgcaaac tggtcgacat
     4141 caccatgacg cagaaccaga tcacctacga tcgtctcaac gtgacgctga cccgtgatga
     4201 cgtgatgggc gaaagcctct acaacccgat gctgccagga attgtggcgg atctcaaagc
     4261 caaaggtctg gcagtagaaa gcgaaggggc gaccgtcgta ttccttgatg agtttaaaaa
     4321 caaggaaggc gaaccgatgg gcgtgatcat tcagaagaaa gatggcggct atctctacac
     4381 caccactgat atcgcctgtg cgaaatatcg ttatgaaaca ctgcatgccg atcgcgtgct
     4441 gtattacatc gactcccgtc agcatcaaca cctgatgcag gcatgggcga tcgtccgtaa
     4501 agcaggctat gtaccggaat ccgtaccgct ggaacaccac atgttcggca tgatgctggg
     4561 taaagacggc aaaccgttca aaacccgcgc gggtggtaca gtgaaactgg ccgatctgct
     4621 ggatgaagcc ctggaacgtg cacgccgtct ggtggcagaa aagaacccgg atatgccagc
     4681 cgacgagctg gaaaaactgg ctaacgcggt tggtattggt gcggtgaaat atgcggatct
     4741 ctccaaaaac cgcaccacgg actacatctt cgactgggac aacatgctgg cgtttgaggg
     4801 taataccgcg ccatacatgc agtatgcata cacgcgtgta ttgtccgtgt tccgtaaagc
     4861 agaaattgac gaagagcaac tggctgcagc tccggttatc atccgtgaag atcgtgaagc
     4921 gcaactggca gctcgcctgc tgcagtttga agaaaccctc accgtggttg cccgtgaagg
     4981 cacgccgcat gtaatgtgtg cttacctgta cgatctggcc ggtctgttct ctggcttcta
     5041 cgagcactgc ccgatcctca gcgcagaaaa cgaagaagtg cgtaacagcc gtctaaaact
     5101 ggcacaactg acggcgaaga cgctgaagct gggtctggat acgctgggta ttgagactgt
     5161 agagcgtatg taagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     5221 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     5281 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
     5341 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5401 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5461 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5521 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5581 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5641 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5701 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5761 ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5821 ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc
     5881 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     5941 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     6001 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     6061 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     6121 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     6181 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     6241 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     6301 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     6361 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     6421 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     6481 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     6541 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     6601 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga
     6661 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     6721 ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6781 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6841 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6901 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     6961 tgtagccgta gttaggccac cacttcaaga actctgtagc
//