LOCUS       pET-28a_(+)_AsnRS       6667 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
KEYWORDS    "accession:addgene_vdb_2565"                                       
FEATURES             Location/Qualifiers
     origin          303..922
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          1321..1343
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         complement(1337..1528)
                     /label="ROP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            complement(2003..2278)
                     /label="tet (576 - 851)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2337..3428
                     /label="Translation 2337-3428"
     regulatory      complement(2337..3428)
                     /label="lacI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            complement(3511..3774)
                     /label="tet (300 - 563)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3703..3722
                     /label="pBRrevBam_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3806..3824
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     regulatory      3824..3851
                     /label="lacO"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     feature         3869..3885
                     /label="T7_transl_en_RBS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3877..3901)
                     /label="Tentative pET28 anneal FOR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3893..3895
                     /label="Start Codon"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3893..5367
                     /label="Translation 3893-5367"
     misc_feature    3905..3922
                     /label="His Tag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     tag             3932..3949
                     /label="Thrombin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3952..3952
                     /label="Xpress_fwd_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(3952..3952)
                     /label="Tim Excises"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            3953..5353
                     /label="asnS gene"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3953..5353
                     /label="asnS CDS (asparaginyl tRNA synthetase)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      5361..5489
                     /label="T7_terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5365..5367
                     /label="Stop Codon"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(5403..5421)
                     /label="T7_Terminal_primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            complement(join(6049..6667,1..197))
                     /label="KanR2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
       61 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
      121 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
      181 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
      241 gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      301 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      361 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      421 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
      481 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      541 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      601 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      661 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      721 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      781 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      841 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      901 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      961 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1021 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1081 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     1141 catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     1201 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     1261 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     1321 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     1381 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     1441 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     1501 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     1561 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     1621 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     1681 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     1741 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     1801 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     1861 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     1921 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     1981 tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     2041 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     2101 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     2161 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     2221 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     2281 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     2341 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2401 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     2461 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     2521 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     2581 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     2641 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     2701 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     2761 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     2821 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     2881 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     2941 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     3001 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     3061 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     3121 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     3181 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     3241 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     3301 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     3361 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     3421 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     3481 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     3541 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     3601 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     3661 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     3721 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     3781 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
     3841 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgggcag
     3901 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatgagcgt
     3961 tgtgcctgta gccgacgtac tccagggccg tgtagccgtt gacagcgaag tcaccgtgcg
     4021 cggatgggta cgtacccgcc gagattcaaa agctggcatc tccttcctcg ccgtttatga
     4081 cggttcctgc tttgatcctg tacaggctgt catcaataat tctctgccca attacaatga
     4141 agacgtcctg cgtctgacca ccggctgctc ggtcattgtg acgggtaaag tcgtggcgtc
     4201 gccgggccag gggcaacaat ttgaaattca ggccagcaag gttgaagttg ctggttgggt
     4261 tgaagatcca gacacttacc cgatggcggc aaaacgccac agcattgagt atctgcgtga
     4321 agtcgctcac ctgcgtccgc gcacaaacct gattggtgcc gtcgcgcgcg ttcgccatac
     4381 gctggcgcag gcgctgcatc gcttctttaa cgagcaggga ttcttctggg tttcaacgcc
     4441 actgattacc gcatctgata ccgaaggtgc aggcgaaatg ttccgcgttt ctacgctgga
     4501 tctggaaaac ctgccgcgta acgatcaggg caaagtggat ttcgacaaag acttctttgg
     4561 taaagagtct ttcctgaccg tatctggcca gttgaacggc gaaacctacg cttgcgcatt
     4621 gtccaaaatt tataccttcg gcccgacttt ccgtgctgaa aactccaaca ccagccgtca
     4681 cctggcggaa ttctggatgc tggagccgga agtggcgttt gctaacctga acgatattgc
     4741 gggtctggct gaagccatgc tgaaatatgt cttcaaagcg gttctcgaag aacgcgctga
     4801 cgacatgaaa ttcttcgctg aacgcgtaga taaagatgcc gtttcacgtc tggaacgctt
     4861 cattgaagcc gattttgcgc aggtggatta taccgacgca gtgaccattc tcgaaaactg
     4921 cggcaggaag tttgaaaacc cggtttactg gggagtcgat ctctcttctg agcatgagcg
     4981 ttatctggcg gaagaacact ttaaagcacc ggtagtggtt aaaaactatc cgaaagatat
     5041 taaagcgttc tatatgcgcc ttaacgaaga cggtaaaacc gttgcggcta tggacgttct
     5101 ggctccgggc atcggtgaga tcattggtgg ctcccagcgt gaagaacgtc tggacgtgct
     5161 ggacgagcgt atgctggaaa tgggcctgaa taaagaagat tactggtggt atcgcgatct
     5221 gcgtcgctac ggtactgttc cgcattcagg tttcggtctt ggttttgaac gtctgattgc
     5281 ttacgtaact ggcgtgcaaa acgtacgtga tgtgattccg ttcccacgta ctccgcgtaa
     5341 cgccagcttc taagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     5401 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     5461 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
     5521 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5581 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5641 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5701 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5761 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5821 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5881 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5941 ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6001 ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc
     6061 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     6121 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     6181 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     6241 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     6301 gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     6361 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     6421 acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     6481 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     6541 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     6601 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     6661 ctcatct
//