LOCUS       pET-28a_(+)_IF1         5485 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
KEYWORDS    "accession:addgene_vdb_2565"                                       
FEATURES             Location/Qualifiers
     origin          423..1042
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          1441..1463
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         complement(1457..1648)
                     /label="ROP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            complement(2123..2398)
                     /label="tet (576 - 851)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     regulatory      complement(2457..3548)
                     /label="lacI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            complement(3631..3894)
                     /label="tet (300 - 563)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3823..3842
                     /label="pBRrevBam_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3926..3944
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     regulatory      3944..3971
                     /label="lacO"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     feature         3989..4005
                     /label="T7_transl_en_RBS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3997..4021)
                     /label="Tentative pET28 anneal FOR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4013..4015
                     /label="Start Codon"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             4013..4305
                     /label="Translation 4013-4305"
     misc_feature    4025..4042
                     /label="His Tag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    4052..4069
                     /label="Thrombin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          4072..4072
                     /label="Xpress_fwd_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4072..4072)
                     /label="Tim Excises"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4073..4075
                     /label="IF1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4073..4291
                     /label="IF1"
     terminator      4299..4427
                     /label="T7_terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4303..4305
                     /label="Stop Codon"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(4341..4359)
                     /label="T7_Terminal_primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            complement(join(4987..5485,1..317))
                     /label="KanR2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
       61 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
      121 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
      181 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
      241 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
      301 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
      361 gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      421 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      481 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      541 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
      601 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      661 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      721 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      781 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      841 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      901 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      961 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1021 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1081 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1141 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1201 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     1261 catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     1321 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     1381 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     1441 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     1501 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     1561 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     1621 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     1681 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     1741 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     1801 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     1861 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     1921 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     1981 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     2041 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     2101 tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     2161 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     2221 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     2281 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     2341 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     2401 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     2461 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2521 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     2581 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     2641 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     2701 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     2761 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     2821 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     2881 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     2941 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     3001 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     3061 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     3121 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     3181 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     3241 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     3301 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     3361 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     3421 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     3481 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     3541 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     3601 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     3661 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     3721 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     3781 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     3841 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     3901 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
     3961 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgggcag
     4021 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatgGCCAA
     4081 AGAAGACAAT ATTGAAATGC AAGGTACCGT TCTTGAAACG TTGCCTAATA CCATGTTCCG
     4141 CGTAGAGTTA GAAAACGGTC ACGTGGTTAC TGCACACATC TCCGGTAAAA TGCGCAAAAA
     4201 CTACATCCGC ATCCTGACGG GCGACAAAGT GACTGTTGAA CTGACCCCGT ACGACCTGAG
     4261 CAAAGGCCGC ATTGTCTTCC GTAGTCGCtg agatccggct gctaacaaag cccgaaagga
     4321 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     4381 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
     4441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4861 gcgaatttta acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg
     4921 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat
     4981 taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     5041 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     5101 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     5161 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     5221 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
     5281 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     5341 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     5401 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     5461 aggatattct tctaatacct ggaat
//