LOCUS       pET-28a_(+)_IF2         7939 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
KEYWORDS    "accession:addgene_vdb_2565"                                       
FEATURES             Location/Qualifiers
     origin          303..922
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          1321..1343
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         complement(1337..1528)
                     /label="ROP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            complement(2003..2278)
                     /label="tet (576 - 851)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2337..3428
                     /label="Translation 2337-3428"
     regulatory      complement(2337..3428)
                     /label="lacI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            complement(3511..3774)
                     /label="tet (300 - 563)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3703..3722
                     /label="pBRrevBam_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3806..3824
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     regulatory      3824..3851
                     /label="lacO"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     feature         3869..3885
                     /label="T7_transl_en_RBS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3877..3901)
                     /label="Tentative pET28 anneal FOR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3893..3895
                     /label="Start Codon"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3893..6639
                     /label="Translation 3893-6639"
     misc_feature    3905..3922
                     /label="His Tag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     tag             3932..3949
                     /label="Thrombin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3952..3952
                     /label="Xpress_fwd_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(3952..3952)
                     /label="Tim Excises"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3953..6625
                     /label="IF2"
     terminator      6633..6761
                     /label="T7_terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6637..6639
                     /label="Stop Codon"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(6675..6693)
                     /label="T7_Terminal_primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            complement(join(7321..7939,1..197))
                     /label="KanR2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
       61 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
      121 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
      181 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
      241 gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      301 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      361 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      421 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
      481 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      541 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      601 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      661 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      721 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      781 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      841 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      901 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      961 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1021 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1081 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     1141 catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     1201 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     1261 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     1321 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     1381 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     1441 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     1501 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     1561 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     1621 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     1681 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     1741 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     1801 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     1861 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     1921 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     1981 tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     2041 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     2101 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     2161 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     2221 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     2281 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     2341 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2401 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     2461 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     2521 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     2581 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     2641 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     2701 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     2761 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     2821 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     2881 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     2941 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     3001 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     3061 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     3121 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     3181 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     3241 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     3301 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     3361 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     3421 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     3481 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     3541 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     3601 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     3661 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     3721 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     3781 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
     3841 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgggcag
     3901 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatgACAGA
     3961 TGTAACGATT AAAACGCTGG CCGCAGAGCG ACAGACCTCC GTGGAACGCC TGGTACAGCA
     4021 ATTTGCTGAT GCAGGTATCC GGAAGTCTGC TGACGACTCT GTGTCTGCAC AAGAGAAACA
     4081 GACTTTGATT GACCACCTGA ATCAGAAAAA TTCAGGCCCG GACAAATTGA CGCTGCAACG
     4141 TAAAACACGC AGCACCCTTA ACATTCCTGG TACCGGTGGA AAAAGCAAAT CGGTACAAAT
     4201 CGAAGTCCGC AAGAAACGCA CCTTTGTGAA ACGCGATCCG CAAGAGGCTG AACGCCTTGC
     4261 AGCGGAAGAG CAAGCGCAGC GTGAAGCGGA AGAGCAAGCC CGTCGTGAGG CAGAAGAATC
     4321 GGCTAAACGC GAGGCGCAAC AAAAAGCTGA ACGTGAGGCC GCAGAACAAG CTAAGCGTGA
     4381 AGCTGCTGAA CAAGCGAAAC GTGAAGCTGC GGAAAAAGAC AAAGTGAGCA ATCAACAAGA
     4441 CGATATGACT AAAAACGCCC AGGCTGAAAA AGCCCGCCGT GAGCAGGAAG CTGCAGAGCT
     4501 CAAGCGTAAA GCTGAAGAAG AAGCGCGTCG TAAACTCGAA GAAGAAGCAC GTCGCGTTGC
     4561 TGAAGAAGCA CGTCGTATGG CGGAAGAAAA CAAATGGACT GATAACGCGG AACCGACTGA
     4621 AGATTCCAGC GATTATCACG TCACTACTTC TCAACATGCT CGCCAGGCAG AAGACGAAAG
     4681 CGATCGTGAA GTCGAAGGCG GCCGTGGCCG TGGTCGTAAC GCGAAAGCAG CGCGTCCGAA
     4741 GAAAGGCAAC AAACACGCTG AATCAAAAGC TGATCGTGAA GAAGCACGCG CAGCAGTACG
     4801 TGGCGGTAAA GGCGGAAAAC GTAAAGGTTC TTCGCTGCAG CAAGGCTTCC AGAAGCCTGC
     4861 TCAGGCCGTT AACCGTGACG TTGTGATCGG CGAAACTATC ACCGTTGGCG AACTGGCGAA
     4921 CAAGATGGCG GTTAAAGGCT CTCAGGTCAT CAAAGCGATG ATGAAACTGG GCGCAATGGC
     4981 AACCATCAAC CAGGTTATCG ATCAGGAAAC CGCACAGCTG GTTGCTGAAG AGATGGGCCA
     5041 TAAAGTTATC CTGCGTCGTG AAAACGAGCT GGAAGAGGCG GTAATGAGCG ACCGTGACAC
     5101 GGGTGCTGCG GCTGAACCGC GCGCGCCGGT TGTGACCATC ATGGGTCACG TTGACCACGG
     5161 TAAAACCTCT CTGCTGGACT ACATTCGTTC AACGAAAGTG GCCTCTGGCG AAGCGGGCGG
     5221 CATTACCCAG CACATTGGTG CATACCACGT TGAAACTGAA AACGGCATGA TCACCTTCCT
     5281 GGACACCCCG GGGCACGCCG CGTTTACTTC AATGCGTGCT CGTGGTGCGC AGGCAACGGA
     5341 CATCGTAGTC CTGGTTGTTG CTGCCGACGA CGGTGTGATG CCGCAGACCA TCGAAGCAAT
     5401 CCAGCACGCG AAAGCGGCGC AGGTACCGGT GGTGGTTGCA GTGAACAAGA TCGATAAACC
     5461 AGAAGCTGAT CCGGATCGCG TTAAGAACGA ACTCTCCCAG TACGGCATCC TGCCGGAAGA
     5521 GTGGGGCGGT GAAAGCCAGT TCGTACACGT ATCTGCGAAA GCGGGTACCG GTATCGATGA
     5581 ACTGCTGGAC GCTATCCTGC TGCAGGCGGA AGTTCTGGAG CTGAAAGCGG TACGTAAAGG
     5641 TATGGCGAGC GGTGCGGTTA TCGAATCCTT CCTCGATAAA GGTCGTGGTC CGGTTGCTAC
     5701 CGTTCTGGTA CGTGAAGGTA CTCTGCACAA GGGCGATATC GTTCTGTGTG GCTTCGAATA
     5761 CGGTCGTGTT CGTGCGATGC GTAACGAACT GGGTCAGGAA GTGCTGGAAG CGGGTCCGTC
     5821 CATTCCGGTG GAAATCCTCG GCCTGTCCGG CGTACCGGCT GCGGGTGATG AAGTTACCGT
     5881 TGTACGTGAC GAGAAGAAAG CGCGTGAAGT TGCACTCTAT CGTCAGGGTA AATTCCGCGA
     5941 AGTTAAACTG GCGCGTCAGC AGAAATCTAA ACTCGAGAAC ATGTTCGCCA ACATGACCGA
     6001 AGGCGAAGTT CACGAAGTGA ATATCGTCCT GAAGGCAGAC GTACAGGGTT CTGTCGAAGC
     6061 GATCTCCGAC TCCTTGCTGA AACTGTCTAC TGACGAAGTT AAAGTGAAGA TCATCGGTTC
     6121 TGGCGTAGGT GGTATCACCG AAACCGACGC CACCCTGGCT GCGGCGTCCA ACGCCATCCT
     6181 GGTTGGCTTT AACGTACGTG CTGATGCCTC TGCACGTAAA GTGATTGAAG CGGAAAGCCT
     6241 GGATCTGCGT TACTACTCCG TCATCTATAA CCTGATTGAC GAAGTGAAAG CGGCGATGAG
     6301 CGGTATGCTG TCTCCGGAAC TGAAACAGCA GATTATCGGT CTGGCGGAAG TTCGTGACGT
     6361 GTTCAAATCG CCGAAATTTG GTGCCATCGC AGGCTGTATG GTTACCGAAG GTGTGGTTAA
     6421 ACGTCACAAC CCGATCCGCG TTCTGCGTGA CAACGTGGTT ATCTACGAAG GCGAGCTGGA
     6481 GTCCCTGCGC CGCTTCAAAG ATGACGTTAA CGAAGTCCGT AACGGTATGG AATGTGGTAT
     6541 CGGCGTTAAG AACTACAACG ACGTCCGCAC TGGCGATGTG ATCGAAGTAT TCGAAATCAT
     6601 CGAGATCCAA CGTACCATTG CTtaagatcc ggctgctaac aaagcccgaa aggaagctga
     6661 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     6721 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
     6781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6841 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6901 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6961 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7021 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7081 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7141 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7201 tttaacaaaa tattaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga
     7261 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     7321 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     7381 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     7441 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     7501 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     7561 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     7621 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     7681 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     7741 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     7801 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     7861 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     7921 tagtctgacc atctcatct
//