LOCUS       pET-28a_(+)_IF3         5809 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
KEYWORDS    "accession:addgene_vdb_2565"                                       
FEATURES             Location/Qualifiers
     gene            complement(717..980)
                     /label="tet (300 - 563)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          909..928
                     /label="pBRrevBam_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        1012..1030
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     regulatory      1030..1057
                     /label="lacO"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     feature         1075..1091
                     /label="T7_transl_en_RBS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(1083..1107)
                     /label="Tentative pET28 anneal FOR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1099..1101
                     /label="Start Codon"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             1099..1715
                     /label="Translation 1099-1715"
     misc_feature    1111..1128
                     /label="His Tag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1138..1155
                     /label="Thrombin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1158..1158
                     /label="Xpress_fwd_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1158..1158)
                     /label="Tim Excises"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1159..1701
                     /label="infC CDS (translation initiation factor IF-3)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            1159..1701
                     /label="infC gene"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      1709..1837
                     /label="T7_terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1713..1715
                     /label="Stop Codon"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(1751..1769)
                     /label="T7_Terminal_primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1811..1837)
                     /label="pET28a-T7 term"
                     /note="sequence: atccggatatagttcctcctttcagca"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     gene            complement(2397..3212)
                     /label="KanR2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     origin          3318..3937
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          4336..4358
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         complement(4352..4543)
                     /label="ROP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            complement(5018..5293)
                     /label="tet (576 - 851)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     regulatory      complement(join(5352..5809,1..634))
                     /label="lacI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
       61 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
      121 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
      181 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
      241 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
      301 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
      361 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
      421 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
      481 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
      541 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
      601 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct
      661 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct
      721 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
      781 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
      841 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
      901 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
      961 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
     1021 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
     1081 ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg
     1141 gtgccgcgcg gcagccatat gaaaggcgga aaacgagttc aaacggcgcg ccctaaccgt
     1201 atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag gtctggaagg cgagcagctt
     1261 ggtattgtga gtctgagaga agctctggag aaagcagaag aagccggagt agacttagtc
     1321 gagatcagcc ctaacgccga gccgccggtt tgtcgtataa tggattacgg caaattcctc
     1381 tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc aaaaagttat ccaggttaag
     1441 gaaattaaat tccgtcctgg tacagatgaa ggcgactatc aggtaaaact ccgcagcctg
     1501 attcgctttc tcgaagaggg tgataaagcc aaaatcacgc tgcgtttccg cggtcgtgag
     1561 atggcgcacc agcaaatcgg tatggaagtg cttaatcgcg tgaaagacga tttgcaagaa
     1621 ctggcagtgg tcgaatcctt cccaacgaag atcgaaggcc gccagatgat catggtgctc
     1681 gctcctaaga agaaacagta agatccggct gctaacaaag cccgaaagga agctgagttg
     1741 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     1801 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
     1861 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1921 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1981 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2041 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     2101 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2161 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     2221 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2281 acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2341 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag
     2401 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     2461 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     2521 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     2581 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     2641 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     2701 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     2761 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     2821 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     2881 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     2941 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     3001 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     3061 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     3121 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
     3181 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     3241 gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3301 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3421 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3481 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3841 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3901 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3961 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4021 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4081 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     4141 cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4201 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     4261 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4321 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4381 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
     4441 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     4501 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     4561 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     4621 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     4681 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
     4741 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
     4801 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     4861 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     4921 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     4981 acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct
     5041 tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga
     5101 ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc
     5161 cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag
     5221 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     5281 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa
     5341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     5461 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     5521 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     5581 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
     5641 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     5701 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     5761 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaattt
//