LOCUS       pOMRP1                  5023 bp ds-DNA     circular     04-JAN-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     promoter        complement(309..413)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    414..428
                     /label="AarI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     429..445
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      446..477
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(474..492)
                     /label="SLiCE-rev"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(474..512)
                     /label="Vector.FOR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     488..512
                     /label="Fragment.FOR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    492..492
                     /label="BioBrick prefix"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(492..512)
                     /label="r_popen2_g3pdp"
                     /note="sequence: AGGGCGTTGACGACGCCTCTgcgatggcgaagactactccc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          513..528
                     /label="F_G3PDp_105971SS1"
                     /note="sequence: AGAGGCGTCGTCAACG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          513..529
                     /label="f_g3pdp2_popen"
                     /note="sequence: ggagtagtcttcgccatcgcAGAGGCGTCGTCAACGC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    513..1722
                     /label="G3PDp_10597SS1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    513..1864
                     /label="G3PD_10597SS1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    642..691
                     /label="Core_putative"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    682..682
                     /label="TSS_putative"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    949..956
                     /label="MEME_motif_putative_G3PD_sdhB"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    961..964
                     /label="TATA_putative"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1400..1403
                     /label="CAAT_putative"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1419..1422
                     /label="CAAT_putative"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1484..1487
                     /label="TATA_putative"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1591..1594
                     /label="CAAT_putative"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          1606..1628
                     /label="seq_RFP_0001"
                     /note="sequence: GCGACGGAGCTATTAGGACATGG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1723..1782
                     /label="5'UTR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1783..1788
                     /label="Exon_1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1789..1844
                     /label="Intron_1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1841..1864)
                     /label="R_G3PDp_1057ss1"
                     /note="sequence: CCGTTAATTCCGACTTTGACCTAG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1845..1864
                     /label="Exon_2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1865..2890
                     /label="hph_GanoLuc.optimized"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1865..2890
                     /label="Translation 1865-2890"
     CDS             1865..2890
                     /label="Translation 1865-2890"
     CDS             1865..2890
                     /label="Translation 1865-2890"
     CDS             1865..2890
                     /label="Translation 1865-2890"
     CDS             1865..2890
                     /label="Translation 1865-2890"
     CDS             1865..2890
                     /label="Translation 1865-2890"
     CDS             1865..2890
                     /label="Translation 1865-2890"
     misc_feature    2891..2969
                     /label="3'UTR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2891..3503
                     /label="G3PD_10597SS1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2970..3503
                     /label="Terminator_polyA_putative"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3074..3098)
                     /label="seqRev_G3PDt_2"
                     /note="sequence: GTATGTACCTCCGGTTTGCGTGATC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          3255..3274
                     /label="seq_bk2_0001"
                     /note="sequence: GCTTCGGGCCATATCAACGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3481..3503)
                     /label="R_G3PDt_10597SS1"
                     /note="sequence: AATCGCGAGCTCACTAATCACCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(3481..3503)
                     /label="r_g3pdt2_popen"
                     /note="sequence: agcgtggtcttcaacatcgcAATCGCGAGCTCACTAATCACCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3504..3509
                     /label="BtgzI rec."
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3504..3516
                     /label="New insert"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          3504..3526
                     /label="f_popen2_g3pdt"
                     /note="sequence: TGATTAGTGAGCTCGCGATTgcgatgttgaagaccacgctgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     3504..3542
                     /label="Vector.REV"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(3504..3548)
                     /label="Fragment.REV"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3517..3519
                     /label="Rev Anneal"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3524..3527
                     /label="BioBrick suffix"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3524..3542
                     /label="SLiCE-for"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(3527..3548)
                     /label="R_pOM_HygR"
                     /note="sequence: cagcggctccgacgattgacac"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(3543..3561)
                     /label="T7 Reverse"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      3557..3604
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(3605..3625)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3626..3640
                     /label="AarI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3641..3691
                     /label="spacer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3692..4280)
                     /label="ori"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     source          join(3989..5023,1..3988)
                     /label="source"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4083..4106
                     /label="BB seq FOR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4257..4284
                     /label="upstream anneal"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4285..4304
                     /label="20bp random sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4305..4331)
                     /label="downstream anneal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(4465..4493)
                     /label="pOpen ori anneal REV"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(join(4471..5023,1..308))
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(4490..4511)
                     /label="BB seq REV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4689..4713
                     /label="popen_midFor"
                     /note="sequence: caactttatccgcctccatccagtc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(4729..4754)
                     /label="popen_midRev"
                     /note="sequence: ctggcgaactacttactctagcttcc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
       61 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
      121 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      181 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      241 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      301 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacctgc
      421 accagtcagt aaaacgacgg ccagtagtca aaagcctccg accggaggct tttgacttgg
      481 tcaggtggag tgggagtagt cttcgccatc gcAGAGGCGT CGTCAACGCC CTCTCCCGAT
      541 GAGATTGTGC GGGTTGACTT GGAGAGCGGG AGGGAGAGTC CGACGGGGAC GGGGGTGAGG
      601 TGACACTTCG GTCCGGGGGT GAATGTCGCA GGAGCGATGA CATCATGATG TGAATATCAG
      661 TCGGCAGGGC ATGGCAAGTG CGTGTGTTAT ACTTGGTGTC TGTGCGGCAA CCAGTGGAAG
      721 CTGACGACTG ATGATGGTGT TTCAGATTTG GGTCCAGCCC GTGCCCTGTC ATCCGAGGAC
      781 CTGCGAATAC TTCAACCTCA TAGGTCGAAT CCATGAGGAC TGATGACAGG AGGTTTCCCG
      841 GATTTTGCGA TTTGATGTGC CTCGGGTGGC ACTCAGACAC TCTAACGCTT ATTCTCGGCC
      901 AGTGCTTTTA TCACTATGTA ATGTGTGCGT TGTGTGTTGC CTGACTTGTT GTACAACCAG
      961 TATAGACTTC ATCGATGTAT GAACCAGGGT GTGACTATTC GCTTGGCTGG GCTCTTACTC
     1021 TTTCTCAGTG GCAAGGTGCC TTCAAGAGCG GTTCCTTGCG GAGCACCACC TGAGCGGCCC
     1081 TGCCCGGGTT CAGGTTGAGC TTGCAGGCTA AACATCGATG TAGGGTTAAT CAGATGTAGA
     1141 ATGCGGTCGC CTTCTGCCTT GCCCGTAGTG CAGGACTACG ACTGCAGCAC CCAACACGAC
     1201 TGTGGAATGG CGAGCAGCAC AGGGCAGCTT CTAACCCATG GAGAGACAAG GGGATGGGCG
     1261 CGCCAGTAAG CGCATATGCC ATTATGGTCC ACTGAGACTG ACCGGTGTAT CCACCAGGCT
     1321 GTCTTTGTTG ACAGATCTAC CCGAACGCTC TCTGTGCGCC GGAGTCGGCG GCCGGCACGG
     1381 AGCACTGGGC CGATTCCGTC AATATGACAA ACCTCCTGCA ATCGAGTCAT GCACTGTGTG
     1441 CTGTGCATTG CGATCGAATC GGAGGCGTCT TCTCTTGTGT GCCTATAGGT TTGGTGAGTT
     1501 TCCCTGTTCC AACGTAACAT TCGTTTGTAT TGCCTTCTGA CCAAGCACCC AGAAGGAGGG
     1561 GGAGGGGGAG GATGACATTT CGGCGTGTAT CAATACGATT ACGTTGCGAC GGAGCTATTA
     1621 GGACATGGTA ATCTACACAC CGAGCCCGAG CGGCCCGCGC TTACAGCGGA CGAGAGGCCA
     1681 TAAAAGCATC GCTGGGTTTC GAAAGTTAGA CCGAAGTCTC ATCTCTGATC TTCATTTCCT
     1741 CTTCATCTAT TTCGACCTAT ACTAGTAACC CTAGTTTCCG CAATGCCCGT GAGTAGATGA
     1801 CTTCCGTTCC TCTTGCGGCG GATTGACCGT ACTGGTACTT CTAGGTCAAA GTCGGAATTA
     1861 ACGGATGAAG AAGCCGGAGC TTACGGCGAC TAGCGTGGAG AAGTTCCTTA TTGAGAAGTT
     1921 CGACTCGGTG AGCGACCTTA TGCAGCTCAG CGAAGGTGAG GAGAGCCGCG CCTTCTCGTT
     1981 CGATGTCGGC GGTCGTGGCT ATGTGCTGCG TGTTAACTCC TGTGCGGACG GGTTCTACAA
     2041 GGACCGCTAC GTGTATCGCC ATTTCGCCTC CGCCGCTCTC CCGATTCCCG AGGTTCTGGA
     2101 CATCGGCGAG TTCTCGGAGT CGCTGACGTA CTGCATCTCC CGCCGCGCTC AAGGCGTTAC
     2161 TCTCCAGGAT CTCCCCGAAA CCGAACTCCC CGCTGTCCTC CAACCTGTCG CGGAAGCTAT
     2221 GGACGCCATT GCTGCCGCGG ATCTCTCCCA GACGTCGGGC TTCGGACCCT TCGGCCCTCA
     2281 GGGCATTGGG CAGTACACGA CGTGGCGTGA CTTCATCTGC GCTATCGCGG ACCCTCATGT
     2341 TTACCACTGG CAGACGGTTA TGGATGACAC CGTGTCGGCG TCGGTTGCGC AGGCGCTTGA
     2401 CGAGCTTATG CTCTGGGCTG AGGATTGTCC CGAGGTGCGT CACCTGGTGC ATGCGGACTT
     2461 CGGCTCCAAC AACGTCCTGA CGGACAATGG GCGCATCACC GCCGTGATTG ATTGGTCCGA
     2521 GGCGATGTTC GGGGATAGCC AGTACGAGGT GGCCAACATC TTCTTCTGGC GCCCCTGGCT
     2581 CGCTTGCATG GAGCAGCAAA CCCGCTACTT CGAGCGCCGT CACCCTGAGC TGGCTGGTAG
     2641 CCCTCGCCTC CGCGCCTATA TGCTGCGCAT CGGcCTCGAC CAGCTCTATC AGTCCCTCGT
     2701 CGACGGCAAC TTCGACGACG CTGCGTGGGC TCAAGGTCGT TGTGACGCTA TCGTTCGTTC
     2761 GGGCGCTGGC ACTGTGGGTC GCACGCAAAT TGCTCGTCGT TCGGCCGCTG TGTGGACGGA
     2821 CGGCTGCGTC GAAGTTCTCG CTGACTCGGG CAACCGCCGC CCAAGCACTC GTCCCCGGGC
     2881 TAAAAAGTGA GTAGAGGCAG ATTGCAAGGT CTTGCTTTGT GCCCAGTGGC CTGAAGGTAT
     2941 CTATGAACTG TAAATAATTC TATCACATAG ACCTCAATAG ATTTCATCAT CTGAACTCCA
     3001 GCCTCTATGG ATATGCGGGT AAGTACTCAC TGAGCAACAG TCCAATAACT CGACGTACTA
     3061 CGCAGACATG GCTGATCACG CAAACCGGAG GTACATACTT AAATAAAACG TTAAAGGCAT
     3121 GAGTCGGACG AACAGTCCGG CCTTTGGGTT ACGCTCACAA CAGCGATACC CCCAATCTTG
     3181 AAAGCTCCTA CTACAGGGAT CGATCACATT GATGATCGCC GCTTCAACGA GGCCTTCCAC
     3241 AGGAAGCACA GTTTGCTTCG GGCCATATCA ACGCATGAAT ACGGATTCAA ATTAGTGTAC
     3301 AATTAAAGTA ATTTAAAGGG TATCGTTCAT ATATCCTGGA AATCTTCATT GGTCATGCCG
     3361 GATTGCTCTG GAGGAGGGGA ACACTATGCG GCTGAGAACG CCATTGTACT TCACTAATCT
     3421 CCGATGGCCA ACGATTTCGA GCTTGGAGCA GTTCTGGGTC CCGGATGTTG AGCACAAGGC
     3481 GGGTGATTAG TGAGCTCGCG ATTgcgatgt tgaagaccac gctgaggtgt caatcgtcgg
     3541 agccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     3601 tttgcatggt catagctgtt tcctgagagc ttggcaggtg atgacacaca ttaacaaatt
     3661 tcgtgaggag tctccagaag aatgccatta atttccatag gctccgcccc cctgacgagc
     3721 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4261 gcagaaaaaa aggatctcaa gaaggcctac tattagcaac aacgatcctt tgatcttttc
     4321 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4381 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4441 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4501 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4561 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg
     4621 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4681 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4741 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4801 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4861 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4921 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4981 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gag
//