LOCUS pOMRP2 4720 bp ds-DNA circular 04-JAN-2021 DEFINITION . SOURCE synthetic DNA construct ORGANISM recombinant plasmid FEATURES Location/Qualifiers source join(148..4720,1..147) /label="source" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 242..265 /label="BB seq FOR" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 416..443 /label="upstream anneal" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 444..463 /label="20bp random sequence" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature complement(464..490) /label="downstream anneal" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(624..652) /label="pOpen ori anneal REV" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS complement(630..1490) /label="AmpR" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature complement(649..670) /label="BB seq REV" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 848..872 /label="popen_midFor" /note="sequence: caactttatccgcctccatccagtc" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer complement(888..913) /label="popen_midRev" /note="sequence: ctggcgaactacttactctagcttcc" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 1287..1312 /label="mfor_popen" /note="sequence: gctcatcattggaaaacgttcttcgg" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer complement(1337..1363) /label="mrev_popen" /note="sequence: gttacatcgaactggatctcaacagcg" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 promoter complement(1491..1595) /label="AmpR promoter" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 1596..1610 /label="AarI" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind 1611..1627 /label="M13 fwd" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 terminator 1628..1659 /label="tonB terminator" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature complement(1656..1674) /label="SLiCE-rev" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer_bind complement(1656..1694) /label="Vector.FOR" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer_bind 1670..1694 /label="Fragment.FOR" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 1674..1674 /label="BioBrick prefix" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer 1695..1710 /label="F_G3PDp_105971SS1" /note="sequence: AGAGGCGTCGTCAACG" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer 1695..1710 /label="F_g3pdp_popen" /note="sequence: accgatatggccagtgtgccAGAGGCGTCGTCAACG" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 1695..2904 /label="G3PDp_10597SS1" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1695..3046 /label="G3PD_10597SS1" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 1824..1873 /label="Core_putative" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 1864..1864 /label="TSS_putative" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 2131..2138 /label="MEME_motif_putative_G3PD_sdhB" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 2143..2146 /label="TATA_putative" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 2582..2585 /label="CAAT_putative" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 2601..2604 /label="CAAT_putative" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2666..2669 /label="TATA_putative" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 2773..2776 /label="CAAT_putative" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 2788..2810 /label="seq_RFP_0001" /note="sequence: GCGACGGAGCTATTAGGACATGG" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 2905..2964 /label="5'UTR" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2965..2970 /label="Exon_1" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 2971..3026 /label="Intron_1" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(3020..3046) /label="r_g3pdp_fugfp" /note="sequence: TCCTCCCCGCTGCTGACCATCCGTTAATTCCGACTTTGACCTAGAAG" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(3023..3046) /label="R_G3PDp_1057ss1" /note="sequence: CCGTTAATTCCGACTTTGACCTAG" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 3027..3046 /label="Exon_2" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer 3047..3065 /label="f_fuGFP_g3pdp" /note="sequence: GTCAAAGTCGGAATTAACGGATGGTCAGCAGCGGGGAGG" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 3047..3769 /label="fuGFP_GL" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 3047..3769 /label="Translation 3047-3769" primer complement(3744..3769) /label="r_fugfp_g3pdt" /note="sequence: ACCTTGCAATCTGCCTCTACTCAGGACTTGTAAAGCTCGTCCATGC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer 3770..3790 /label="f_g3pdt_fugfp" /note="sequence: ACGAGCTTTACAAGTCCTGAGTAGAGGCAGATTGCAAGGTC" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 3770..3848 /label="3'UTR" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 3770..4382 /label="G3PD_10597SS1" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 3849..4382 /label="Terminator_polyA_putative" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(3953..3977) /label="seqRev_G3PDt_2" /note="sequence: GTATGTACCTCCGGTTTGCGTGATC" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 4134..4153 /label="seq_bk2_0001" /note="sequence: GCTTCGGGCCATATCAACGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(4360..4382) /label="R_G3PDt_pCbxOH" /note="sequence: tgatccaagctcaagctgctAATCGCGAGCTCACTAATCACCC" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(4360..4382) /label="R_G3PDt_10597SS1" /note="sequence: AATCGCGAGCTCACTAATCACCC" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer complement(4360..4382) /label="R_g3pdt_popen" /note="sequence: ccacttcttccccgataacgAATCGCGAGCTCACTAATCACCC" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 4383..4388 /label="BtgzI rec." /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 4383..4395 /label="New insert" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind 4383..4421 /label="Vector.REV" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer_bind complement(4383..4427) /label="Fragment.REV" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 4396..4398 /label="Rev Anneal" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 4403..4406 /label="BioBrick suffix" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 4403..4421 /label="SLiCE-for" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer_bind complement(4422..4440) /label="T7 Reverse" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 terminator 4436..4483 /label="T7 terminator" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind complement(4484..4504) /label="M13 rev" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 4505..4519 /label="AarI" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 4520..4570 /label="spacer" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin complement(join(4571..4720,1..439)) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 ORIGIN 1 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 61 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 121 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 181 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 241 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 301 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 361 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 421 cagaaaaaaa ggatctcaag aaggcctact attagcaaca acgatccttt gatcttttct 481 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 541 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 601 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 661 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 721 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc 781 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 841 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 901 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 961 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 1021 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 1081 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 1141 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 1201 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 1261 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 1321 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 1381 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 1441 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 1501 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 1561 tgtatttaga aaaataaaca aataggggtt ccgcgcacct gcaccagtca gtaaaacgac 1621 ggccagtagt caaaagcctc cgaccggagg cttttgactt ggtcaggtgg agtgggagta 1681 gtcttcgcca tcgcAGAGGC GTCGTCAACG CCCTCTCCCG ATGAGATTGT GCGGGTTGAC 1741 TTGGAGAGCG GGAGGGAGAG TCCGACGGGG ACGGGGGTGA GGTGACACTT CGGTCCGGGG 1801 GTGAATGTCG CAGGAGCGAT GACATCATGA TGTGAATATC AGTCGGCAGG GCATGGCAAG 1861 TGCGTGTGTT ATACTTGGTG TCTGTGCGGC AACCAGTGGA AGCTGACGAC TGATGATGGT 1921 GTTTCAGATT TGGGTCCAGC CCGTGCCCTG TCATCCGAGG ACCTGCGAAT ACTTCAACCT 1981 CATAGGTCGA ATCCATGAGG ACTGATGACA GGAGGTTTCC CGGATTTTGC GATTTGATGT 2041 GCCTCGGGTG GCACTCAGAC ACTCTAACGC TTATTCTCGG CCAGTGCTTT TATCACTATG 2101 TAATGTGTGC GTTGTGTGTT GCCTGACTTG TTGTACAACC AGTATAGACT TCATCGATGT 2161 ATGAACCAGG GTGTGACTAT TCGCTTGGCT GGGCTCTTAC TCTTTCTCAG TGGCAAGGTG 2221 CCTTCAAGAG CGGTTCCTTG CGGAGCACCA CCTGAGCGGC CCTGCCCGGG TTCAGGTTGA 2281 GCTTGCAGGC TAAACATCGA TGTAGGGTTA ATCAGATGTA GAATGCGGTC GCCTTCTGCC 2341 TTGCCCGTAG TGCAGGACTA CGACTGCAGC ACCCAACACG ACTGTGGAAT GGCGAGCAGC 2401 ACAGGGCAGC TTCTAACCCA TGGAGAGACA AGGGGATGGG CGCGCCAGTA AGCGCATATG 2461 CCATTATGGT CCACTGAGAC TGACCGGTGT ATCCACCAGG CTGTCTTTGT TGACAGATCT 2521 ACCCGAACGC TCTCTGTGCG CCGGAGTCGG CGGCCGGCAC GGAGCACTGG GCCGATTCCG 2581 TCAATATGAC AAACCTCCTG CAATCGAGTC ATGCACTGTG TGCTGTGCAT TGCGATCGAA 2641 TCGGAGGCGT CTTCTCTTGT GTGCCTATAG GTTTGGTGAG TTTCCCTGTT CCAACGTAAC 2701 ATTCGTTTGT ATTGCCTTCT GACCAAGCAC CCAGAAGGAG GGGGAGGGGG AGGATGACAT 2761 TTCGGCGTGT ATCAATACGA TTACGTTGCG ACGGAGCTAT TAGGACATGG TAATCTACAC 2821 ACCGAGCCCG AGCGGCCCGC GCTTACAGCG GACGAGAGGC CATAAAAGCA TCGCTGGGTT 2881 TCGAAAGTTA GACCGAAGTC TCATCTCTGA TCTTCATTTC CTCTTCATCT ATTTCGACCT 2941 ATACTAGTAA CCCTAGTTTC CGCAATGCCC GTGAGTAGAT GACTTCCGTT CCTCTTGCGG 3001 CGGATTGACC GTACTGGTAC TTCTAGGTCA AAGTCGGAAT TAACGGATGG TCAGCAGCGG 3061 GGAGGATATT TTCTCGGGAC TCGTCCCCAT TCTTATCGAA CTGGAGGGCG ACGTCAACGG 3121 GCACCGTTTC TCGGTCCGCG GCGAGGGATA TGGAGACGCC TCCAACGGCA AGCTGGAGAT 3181 CAAGTTCATC TGTACTACTG GCCGCCTCCC GGTCCCTTGG CCTACCCTTG TGACTACCCT 3241 GAGCTACGGG GTCCAGTGTT TCGCCAAGTA CCCTGAGCAC ATGCGGCAGA ACGACTTCTT 3301 CAAGTCCGCG ATGCCGGACG GCTACGTCCA GGAGCGTACC ATCTCCTTCA AGGAAGACGG 3361 AACCTACAAA ACTCGCGCGG AGGTCAAATT CGAGGGGGAG GCCCTCGTGA ACCGCATCGA 3421 TCTCAAGGGG CTCGAGTTCA AAGAGGACGG GAACATTCTC GGACACAAGC TCGAATACTC 3481 GTTCAACTCG CACTACGTCT ATATCACCGC CGACAAGAAC CGCAATGGGC TCGAAGCGCA 3541 GTTCCGCATC CGCCACAACG TGGACGACGG TTCGGTCCAA CTTGCCGACC ACTACCAGCA 3601 GAACACGCCC ATCGGCGAGG GGCCGGTCCT TCTGCCTGAA CAGCACTACC TCACGACGAA 3661 CTCCGTGCTC TCCAAGGATC CTCAGGAGCG CCGCGATCAT ATGGTCCTTG TGGAATTCGT 3721 CACGGCGGCG GGCCTGTCCC TTGGCATGGA CGAGCTTTAC AAGTCCTGAG TAGAGGCAGA 3781 TTGCAAGGTC TTGCTTTGTG CCCAGTGGCC TGAAGGTATC TATGAACTGT AAATAATTCT 3841 ATCACATAGA CCTCAATAGA TTTCATCATC TGAACTCCAG CCTCTATGGA TATGCGGGTA 3901 AGTACTCACT GAGCAACAGT CCAATAACTC GACGTACTAC GCAGACATGG CTGATCACGC 3961 AAACCGGAGG TACATACTTA AATAAAACGT TAAAGGCATG AGTCGGACGA ACAGTCCGGC 4021 CTTTGGGTTA CGCTCACAAC AGCGATACCC CCAATCTTGA AAGCTCCTAC TACAGGGATC 4081 GATCACATTG ATGATCGCCG CTTCAACGAG GCCTTCCACA GGAAGCACAG TTTGCTTCGG 4141 GCCATATCAA CGCATGAATA CGGATTCAAA TTAGTGTACA ATTAAAGTAA TTTAAAGGGT 4201 ATCGTTCATA TATCCTGGAA ATCTTCATTG GTCATGCCGG ATTGCTCTGG AGGAGGGGAA 4261 CACTATGCGG CTGAGAACGC CATTGTACTT CACTAATCTC CGATGGCCAA CGATTTCGAG 4321 CTTGGAGCAG TTCTGGGTCC CGGATGTTGA GCACAAGGCG GGTGATTAGT GAGCTCGCGA 4381 TTgcgatgtt gaagaccacg ctgaggtgtc aatcgtcgga gccgctgagc aataactagc 4441 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgcatggtc atagctgttt 4501 cctgagagct tggcaggtga tgacacacat taacaaattt cgtgaggagt ctccagaaga 4561 atgccattaa tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 4621 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 4681 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg //