LOCUS       pOMRP2                  4720 bp ds-DNA     circular     04-JAN-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     source          join(148..4720,1..147)
                     /label="source"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    242..265
                     /label="BB seq FOR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    416..443
                     /label="upstream anneal"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    444..463
                     /label="20bp random sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(464..490)
                     /label="downstream anneal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(624..652)
                     /label="pOpen ori anneal REV"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(630..1490)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(649..670)
                     /label="BB seq REV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          848..872
                     /label="popen_midFor"
                     /note="sequence: caactttatccgcctccatccagtc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(888..913)
                     /label="popen_midRev"
                     /note="sequence: ctggcgaactacttactctagcttcc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1287..1312
                     /label="mfor_popen"
                     /note="sequence: gctcatcattggaaaacgttcttcgg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1337..1363)
                     /label="mrev_popen"
                     /note="sequence: gttacatcgaactggatctcaacagcg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        complement(1491..1595)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1596..1610
                     /label="AarI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1611..1627
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      1628..1659
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1656..1674)
                     /label="SLiCE-rev"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(1656..1694)
                     /label="Vector.FOR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     1670..1694
                     /label="Fragment.FOR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1674..1674
                     /label="BioBrick prefix"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1695..1710
                     /label="F_G3PDp_105971SS1"
                     /note="sequence: AGAGGCGTCGTCAACG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1695..1710
                     /label="F_g3pdp_popen"
                     /note="sequence: accgatatggccagtgtgccAGAGGCGTCGTCAACG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1695..2904
                     /label="G3PDp_10597SS1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1695..3046
                     /label="G3PD_10597SS1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1824..1873
                     /label="Core_putative"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1864..1864
                     /label="TSS_putative"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2131..2138
                     /label="MEME_motif_putative_G3PD_sdhB"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2143..2146
                     /label="TATA_putative"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2582..2585
                     /label="CAAT_putative"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2601..2604
                     /label="CAAT_putative"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2666..2669
                     /label="TATA_putative"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2773..2776
                     /label="CAAT_putative"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2788..2810
                     /label="seq_RFP_0001"
                     /note="sequence: GCGACGGAGCTATTAGGACATGG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2905..2964
                     /label="5'UTR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2965..2970
                     /label="Exon_1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2971..3026
                     /label="Intron_1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3020..3046)
                     /label="r_g3pdp_fugfp"
                     /note="sequence: TCCTCCCCGCTGCTGACCATCCGTTAATTCCGACTTTGACCTAGAAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(3023..3046)
                     /label="R_G3PDp_1057ss1"
                     /note="sequence: CCGTTAATTCCGACTTTGACCTAG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3027..3046
                     /label="Exon_2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          3047..3065
                     /label="f_fuGFP_g3pdp"
                     /note="sequence: GTCAAAGTCGGAATTAACGGATGGTCAGCAGCGGGGAGG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3047..3769
                     /label="fuGFP_GL"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3047..3769
                     /label="Translation 3047-3769"
     primer          complement(3744..3769)
                     /label="r_fugfp_g3pdt"
                     /note="sequence: ACCTTGCAATCTGCCTCTACTCAGGACTTGTAAAGCTCGTCCATGC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          3770..3790
                     /label="f_g3pdt_fugfp"
                     /note="sequence: ACGAGCTTTACAAGTCCTGAGTAGAGGCAGATTGCAAGGTC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3770..3848
                     /label="3'UTR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3770..4382
                     /label="G3PD_10597SS1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3849..4382
                     /label="Terminator_polyA_putative"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3953..3977)
                     /label="seqRev_G3PDt_2"
                     /note="sequence: GTATGTACCTCCGGTTTGCGTGATC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          4134..4153
                     /label="seq_bk2_0001"
                     /note="sequence: GCTTCGGGCCATATCAACGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(4360..4382)
                     /label="R_G3PDt_pCbxOH"
                     /note="sequence: tgatccaagctcaagctgctAATCGCGAGCTCACTAATCACCC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(4360..4382)
                     /label="R_G3PDt_10597SS1"
                     /note="sequence: AATCGCGAGCTCACTAATCACCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(4360..4382)
                     /label="R_g3pdt_popen"
                     /note="sequence: ccacttcttccccgataacgAATCGCGAGCTCACTAATCACCC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4383..4388
                     /label="BtgzI rec."
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4383..4395
                     /label="New insert"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     4383..4421
                     /label="Vector.REV"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(4383..4427)
                     /label="Fragment.REV"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4396..4398
                     /label="Rev Anneal"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4403..4406
                     /label="BioBrick suffix"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4403..4421
                     /label="SLiCE-for"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(4422..4440)
                     /label="T7 Reverse"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      4436..4483
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(4484..4504)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4505..4519
                     /label="AarI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4520..4570
                     /label="spacer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(join(4571..4720,1..439))
                     /label="ori"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
       61 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      121 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      181 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      241 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      301 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      361 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      421 cagaaaaaaa ggatctcaag aaggcctact attagcaaca acgatccttt gatcttttct
      481 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      541 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      601 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      661 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      721 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agaaccacgc
      781 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      841 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      901 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      961 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1021 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1081 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1141 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1201 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1261 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1321 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1381 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1441 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1501 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1561 tgtatttaga aaaataaaca aataggggtt ccgcgcacct gcaccagtca gtaaaacgac
     1621 ggccagtagt caaaagcctc cgaccggagg cttttgactt ggtcaggtgg agtgggagta
     1681 gtcttcgcca tcgcAGAGGC GTCGTCAACG CCCTCTCCCG ATGAGATTGT GCGGGTTGAC
     1741 TTGGAGAGCG GGAGGGAGAG TCCGACGGGG ACGGGGGTGA GGTGACACTT CGGTCCGGGG
     1801 GTGAATGTCG CAGGAGCGAT GACATCATGA TGTGAATATC AGTCGGCAGG GCATGGCAAG
     1861 TGCGTGTGTT ATACTTGGTG TCTGTGCGGC AACCAGTGGA AGCTGACGAC TGATGATGGT
     1921 GTTTCAGATT TGGGTCCAGC CCGTGCCCTG TCATCCGAGG ACCTGCGAAT ACTTCAACCT
     1981 CATAGGTCGA ATCCATGAGG ACTGATGACA GGAGGTTTCC CGGATTTTGC GATTTGATGT
     2041 GCCTCGGGTG GCACTCAGAC ACTCTAACGC TTATTCTCGG CCAGTGCTTT TATCACTATG
     2101 TAATGTGTGC GTTGTGTGTT GCCTGACTTG TTGTACAACC AGTATAGACT TCATCGATGT
     2161 ATGAACCAGG GTGTGACTAT TCGCTTGGCT GGGCTCTTAC TCTTTCTCAG TGGCAAGGTG
     2221 CCTTCAAGAG CGGTTCCTTG CGGAGCACCA CCTGAGCGGC CCTGCCCGGG TTCAGGTTGA
     2281 GCTTGCAGGC TAAACATCGA TGTAGGGTTA ATCAGATGTA GAATGCGGTC GCCTTCTGCC
     2341 TTGCCCGTAG TGCAGGACTA CGACTGCAGC ACCCAACACG ACTGTGGAAT GGCGAGCAGC
     2401 ACAGGGCAGC TTCTAACCCA TGGAGAGACA AGGGGATGGG CGCGCCAGTA AGCGCATATG
     2461 CCATTATGGT CCACTGAGAC TGACCGGTGT ATCCACCAGG CTGTCTTTGT TGACAGATCT
     2521 ACCCGAACGC TCTCTGTGCG CCGGAGTCGG CGGCCGGCAC GGAGCACTGG GCCGATTCCG
     2581 TCAATATGAC AAACCTCCTG CAATCGAGTC ATGCACTGTG TGCTGTGCAT TGCGATCGAA
     2641 TCGGAGGCGT CTTCTCTTGT GTGCCTATAG GTTTGGTGAG TTTCCCTGTT CCAACGTAAC
     2701 ATTCGTTTGT ATTGCCTTCT GACCAAGCAC CCAGAAGGAG GGGGAGGGGG AGGATGACAT
     2761 TTCGGCGTGT ATCAATACGA TTACGTTGCG ACGGAGCTAT TAGGACATGG TAATCTACAC
     2821 ACCGAGCCCG AGCGGCCCGC GCTTACAGCG GACGAGAGGC CATAAAAGCA TCGCTGGGTT
     2881 TCGAAAGTTA GACCGAAGTC TCATCTCTGA TCTTCATTTC CTCTTCATCT ATTTCGACCT
     2941 ATACTAGTAA CCCTAGTTTC CGCAATGCCC GTGAGTAGAT GACTTCCGTT CCTCTTGCGG
     3001 CGGATTGACC GTACTGGTAC TTCTAGGTCA AAGTCGGAAT TAACGGATGG TCAGCAGCGG
     3061 GGAGGATATT TTCTCGGGAC TCGTCCCCAT TCTTATCGAA CTGGAGGGCG ACGTCAACGG
     3121 GCACCGTTTC TCGGTCCGCG GCGAGGGATA TGGAGACGCC TCCAACGGCA AGCTGGAGAT
     3181 CAAGTTCATC TGTACTACTG GCCGCCTCCC GGTCCCTTGG CCTACCCTTG TGACTACCCT
     3241 GAGCTACGGG GTCCAGTGTT TCGCCAAGTA CCCTGAGCAC ATGCGGCAGA ACGACTTCTT
     3301 CAAGTCCGCG ATGCCGGACG GCTACGTCCA GGAGCGTACC ATCTCCTTCA AGGAAGACGG
     3361 AACCTACAAA ACTCGCGCGG AGGTCAAATT CGAGGGGGAG GCCCTCGTGA ACCGCATCGA
     3421 TCTCAAGGGG CTCGAGTTCA AAGAGGACGG GAACATTCTC GGACACAAGC TCGAATACTC
     3481 GTTCAACTCG CACTACGTCT ATATCACCGC CGACAAGAAC CGCAATGGGC TCGAAGCGCA
     3541 GTTCCGCATC CGCCACAACG TGGACGACGG TTCGGTCCAA CTTGCCGACC ACTACCAGCA
     3601 GAACACGCCC ATCGGCGAGG GGCCGGTCCT TCTGCCTGAA CAGCACTACC TCACGACGAA
     3661 CTCCGTGCTC TCCAAGGATC CTCAGGAGCG CCGCGATCAT ATGGTCCTTG TGGAATTCGT
     3721 CACGGCGGCG GGCCTGTCCC TTGGCATGGA CGAGCTTTAC AAGTCCTGAG TAGAGGCAGA
     3781 TTGCAAGGTC TTGCTTTGTG CCCAGTGGCC TGAAGGTATC TATGAACTGT AAATAATTCT
     3841 ATCACATAGA CCTCAATAGA TTTCATCATC TGAACTCCAG CCTCTATGGA TATGCGGGTA
     3901 AGTACTCACT GAGCAACAGT CCAATAACTC GACGTACTAC GCAGACATGG CTGATCACGC
     3961 AAACCGGAGG TACATACTTA AATAAAACGT TAAAGGCATG AGTCGGACGA ACAGTCCGGC
     4021 CTTTGGGTTA CGCTCACAAC AGCGATACCC CCAATCTTGA AAGCTCCTAC TACAGGGATC
     4081 GATCACATTG ATGATCGCCG CTTCAACGAG GCCTTCCACA GGAAGCACAG TTTGCTTCGG
     4141 GCCATATCAA CGCATGAATA CGGATTCAAA TTAGTGTACA ATTAAAGTAA TTTAAAGGGT
     4201 ATCGTTCATA TATCCTGGAA ATCTTCATTG GTCATGCCGG ATTGCTCTGG AGGAGGGGAA
     4261 CACTATGCGG CTGAGAACGC CATTGTACTT CACTAATCTC CGATGGCCAA CGATTTCGAG
     4321 CTTGGAGCAG TTCTGGGTCC CGGATGTTGA GCACAAGGCG GGTGATTAGT GAGCTCGCGA
     4381 TTgcgatgtt gaagaccacg ctgaggtgtc aatcgtcgga gccgctgagc aataactagc
     4441 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgcatggtc atagctgttt
     4501 cctgagagct tggcaggtga tgacacacat taacaaattt cgtgaggagt ctccagaaga
     4561 atgccattaa tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4621 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4681 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
//