LOCUS       pOMRP3                  4513 bp ds-DNA     circular     04-JAN-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  recombinant plasmid                                                
FEATURES             Location/Qualifiers
     source          1..4513
                     /label="source"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    95..118
                     /label="BB seq FOR"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    269..296
                     /label="upstream anneal"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    297..316
                     /label="20bp random sequence"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(317..343)
                     /label="downstream anneal"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(477..505)
                     /label="pOpen ori anneal REV"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(483..1343)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(502..523)
                     /label="BB seq REV"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          701..725
                     /label="popen_midFor"
                     /note="sequence: caactttatccgcctccatccagtc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(741..766)
                     /label="popen_midRev"
                     /note="sequence: ctggcgaactacttactctagcttcc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(1344..1448)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1449..1463
                     /label="AarI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1464..1480
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     terminator      1481..1512
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1509..1527)
                     /label="SLiCE-rev"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(1509..1547)
                     /label="Vector.FOR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     1523..1547
                     /label="Fragment.FOR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1527..1527
                     /label="BioBrick prefix"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1548..1563
                     /label="F_g3pdp_popen"
                     /note="sequence: accgatatggccagtgtgccAGAGGCGTCGTCAACG"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          1548..1563
                     /label="F_G3PDp_105971SS1"
                     /note="sequence: AGAGGCGTCGTCAACG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1548..2757
                     /label="G3PDp_10597SS1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1548..2899
                     /label="G3PD_10597SS1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1677..1726
                     /label="Core_putative"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1717..1717
                     /label="TSS_putative"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1984..1991
                     /label="MEME_motif_putative_G3PD_sdhB"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1996..1999
                     /label="TATA_putative"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2435..2438
                     /label="CAAT_putative"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2454..2457
                     /label="CAAT_putative"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2519..2522
                     /label="TATA_putative"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2626..2629
                     /label="CAAT_putative"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2641..2663
                     /label="seq_RFP_0001"
                     /note="sequence: GCGACGGAGCTATTAGGACATGG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2758..2817
                     /label="5'UTR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2818..2823
                     /label="Exon_1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2824..2879
                     /label="Intron_1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2873..2899)
                     /label="r_g3pdp_nanoluc"
                     /note="sequence: TCTTCGAGTGTGAAGACCATCCGTTAATTCCGACTTTGACCTAGAAG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2876..2899)
                     /label="R_G3PDp_1057ss1"
                     /note="sequence: CCGTTAATTCCGACTTTGACCTAG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2880..2899
                     /label="Exon_2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          2900..2924
                     /label="f_nanoluc_g3pdp"
                     /note="sequence: GTCAAAGTCGGAATTAACGGATGGTCTTCACACTCGAAGATTTCG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2900..3415
                     /label="NanoLuc_GL_IDToptimized"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             2900..3415
                     /label="Translation 2900-3415"
     primer          complement(3396..3415)
                     /label="r_nanoluc_g3pdt"
                     /note="sequence: ACCTTGCAATCTGCCTCTACTTACGCCAGAATGCGTTCGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          3416..3439
                     /label="f_g3pdt_nanoluc"
                     /note="sequence: GCGAACGCATTCTGGCGTAAGTAGAGGCAGATTGCAAGGTCTTG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3416..3494
                     /label="3'UTR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3416..4028
                     /label="G3PD_10597SS1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3495..4028
                     /label="Terminator_polyA_putative"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3599..3623)
                     /label="seqRev_G3PDt_2"
                     /note="sequence: GTATGTACCTCCGGTTTGCGTGATC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          3780..3799
                     /label="seq_bk2_0001"
                     /note="sequence: GCTTCGGGCCATATCAACGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(4006..4028)
                     /label="R_G3PDt_10597SS1"
                     /note="sequence: AATCGCGAGCTCACTAATCACCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(4006..4028)
                     /label="R_G3PDt_pCbxOH"
                     /note="sequence: tgatccaagctcaagctgctAATCGCGAGCTCACTAATCACCC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(4006..4028)
                     /label="R_g3pdt_popen"
                     /note="sequence: ccacttcttccccgataacgAATCGCGAGCTCACTAATCACCC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4029..4034
                     /label="BtgzI rec."
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4029..4041
                     /label="New insert"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     4029..4067
                     /label="Vector.REV"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(4029..4073)
                     /label="Fragment.REV"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4042..4044
                     /label="Rev Anneal"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4049..4052
                     /label="BioBrick suffix"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4049..4067
                     /label="SLiCE-for"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(4068..4086)
                     /label="T7 Reverse"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      4082..4129
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(4130..4150)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4151..4165
                     /label="AarI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4166..4216
                     /label="spacer"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(join(4217..4513,1..292))
                     /label="ori"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
       61 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      121 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaaggcct
      301 actattagca acaacgatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
      481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
      541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
      601 cccagtgctg caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata
      661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
      721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
      781 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
      841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
      901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
      961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1081 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1441 gttccgcgca cctgcaccag tcagtaaaac gacggccagt agtcaaaagc ctccgaccgg
     1501 aggcttttga cttggtcagg tggagtggga gtagtcttcg ccatcgcAGA GGCGTCGTCA
     1561 ACGCCCTCTC CCGATGAGAT TGTGCGGGTT GACTTGGAGA GCGGGAGGGA GAGTCCGACG
     1621 GGGACGGGGG TGAGGTGACA CTTCGGTCCG GGGGTGAATG TCGCAGGAGC GATGACATCA
     1681 TGATGTGAAT ATCAGTCGGC AGGGCATGGC AAGTGCGTGT GTTATACTTG GTGTCTGTGC
     1741 GGCAACCAGT GGAAGCTGAC GACTGATGAT GGTGTTTCAG ATTTGGGTCC AGCCCGTGCC
     1801 CTGTCATCCG AGGACCTGCG AATACTTCAA CCTCATAGGT CGAATCCATG AGGACTGATG
     1861 ACAGGAGGTT TCCCGGATTT TGCGATTTGA TGTGCCTCGG GTGGCACTCA GACACTCTAA
     1921 CGCTTATTCT CGGCCAGTGC TTTTATCACT ATGTAATGTG TGCGTTGTGT GTTGCCTGAC
     1981 TTGTTGTACA ACCAGTATAG ACTTCATCGA TGTATGAACC AGGGTGTGAC TATTCGCTTG
     2041 GCTGGGCTCT TACTCTTTCT CAGTGGCAAG GTGCCTTCAA GAGCGGTTCC TTGCGGAGCA
     2101 CCACCTGAGC GGCCCTGCCC GGGTTCAGGT TGAGCTTGCA GGCTAAACAT CGATGTAGGG
     2161 TTAATCAGAT GTAGAATGCG GTCGCCTTCT GCCTTGCCCG TAGTGCAGGA CTACGACTGC
     2221 AGCACCCAAC ACGACTGTGG AATGGCGAGC AGCACAGGGC AGCTTCTAAC CCATGGAGAG
     2281 ACAAGGGGAT GGGCGCGCCA GTAAGCGCAT ATGCCATTAT GGTCCACTGA GACTGACCGG
     2341 TGTATCCACC AGGCTGTCTT TGTTGACAGA TCTACCCGAA CGCTCTCTGT GCGCCGGAGT
     2401 CGGCGGCCGG CACGGAGCAC TGGGCCGATT CCGTCAATAT GACAAACCTC CTGCAATCGA
     2461 GTCATGCACT GTGTGCTGTG CATTGCGATC GAATCGGAGG CGTCTTCTCT TGTGTGCCTA
     2521 TAGGTTTGGT GAGTTTCCCT GTTCCAACGT AACATTCGTT TGTATTGCCT TCTGACCAAG
     2581 CACCCAGAAG GAGGGGGAGG GGGAGGATGA CATTTCGGCG TGTATCAATA CGATTACGTT
     2641 GCGACGGAGC TATTAGGACA TGGTAATCTA CACACCGAGC CCGAGCGGCC CGCGCTTACA
     2701 GCGGACGAGA GGCCATAAAA GCATCGCTGG GTTTCGAAAG TTAGACCGAA GTCTCATCTC
     2761 TGATCTTCAT TTCCTCTTCA TCTATTTCGA CCTATACTAG TAACCCTAGT TTCCGCAATG
     2821 CCCGTGAGTA GATGACTTCC GTTCCTCTTG CGGCGGATTG ACCGTACTGG TACTTCTAGG
     2881 TCAAAGTCGG AATTAACGGA TGGTCTTCAC ACTCGAAGAT TTCGTTGGGG ACTGGCGACA
     2941 GACAGCCGGC TACAACCTGG ACCAAGTCCT TGAACAGGGA GGTGTGTCCA GTTTGTTTCA
     3001 GAATCTCGGG GTGTCCGTAA CTCCGATCCA AAGGATTGTC CTGAGCGGTG AAAATGGGCT
     3061 GAAGATCGAC ATCCATGTCA TCATCCCGTA TGAAGGTCTG AGCGGCGACC AAATGGGCCA
     3121 GATCGAAAAA ATTTTTAAGG TGGTGTACCC TGTGGATGAT CATCACTTTA AGGTGATCCT
     3181 GCACTATGGC ACACTGGTAA TCGACGGGGT TACGCCGAAC ATGATCGACT ATTTCGGACG
     3241 GCCGTATGAA GGCATCGCCG TGTTCGACGG CAAAAAGATC ACTGTAACAG GGACCCTGTG
     3301 GAACGGCAAC AAAATTATCG ACGAGCGCCT GATCAACCCC GACGGCTCCC TGCTGTTCCG
     3361 AGTAACCATC AACGGAGTGA CCGGCTGGCG GCTGTGCGAA CGCATTCTGG CGTAAGTAGA
     3421 GGCAGATTGC AAGGTCTTGC TTTGTGCCCA GTGGCCTGAA GGTATCTATG AACTGTAAAT
     3481 AATTCTATCA CATAGACCTC AATAGATTTC ATCATCTGAA CTCCAGCCTC TATGGATATG
     3541 CGGGTAAGTA CTCACTGAGC AACAGTCCAA TAACTCGACG TACTACGCAG ACATGGCTGA
     3601 TCACGCAAAC CGGAGGTACA TACTTAAATA AAACGTTAAA GGCATGAGTC GGACGAACAG
     3661 TCCGGCCTTT GGGTTACGCT CACAACAGCG ATACCCCCAA TCTTGAAAGC TCCTACTACA
     3721 GGGATCGATC ACATTGATGA TCGCCGCTTC AACGAGGCCT TCCACAGGAA GCACAGTTTG
     3781 CTTCGGGCCA TATCAACGCA TGAATACGGA TTCAAATTAG TGTACAATTA AAGTAATTTA
     3841 AAGGGTATCG TTCATATATC CTGGAAATCT TCATTGGTCA TGCCGGATTG CTCTGGAGGA
     3901 GGGGAACACT ATGCGGCTGA GAACGCCATT GTACTTCACT AATCTCCGAT GGCCAACGAT
     3961 TTCGAGCTTG GAGCAGTTCT GGGTCCCGGA TGTTGAGCAC AAGGCGGGTG ATTAGTGAGC
     4021 TCGCGATTgc gatgttgaag accacgctga ggtgtcaatc gtcggagccg ctgagcaata
     4081 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc atggtcatag
     4141 ctgtttcctg agagcttggc aggtgatgac acacattaac aaatttcgtg aggagtctcc
     4201 agaagaatgc cattaatttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4501 gcgccttatc cgg
//