LOCUS       pET-28a_(+)_PheRS       6250 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
KEYWORDS    "accession:addgene_vdb_2565"                                       
FEATURES             Location/Qualifiers
     origin          303..922
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          1321..1343
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         complement(1337..1528)
                     /label="ROP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            complement(2003..2278)
                     /label="tet (576 - 851)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             2337..3428
                     /label="Translation 2337-3428"
     regulatory      complement(2337..3428)
                     /label="lacI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            complement(3511..3774)
                     /label="tet (300 - 563)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3703..3722
                     /label="pBRrevBam_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3806..3824
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     regulatory      3824..3851
                     /label="lacO"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     feature         3869..3885
                     /label="T7_transl_en_RBS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3877..3901)
                     /label="Tentative pET28 anneal FOR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3893..3895
                     /label="Start Codon"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             3893..4950
                     /label="Translation 3893-4950"
     misc_feature    3905..3922
                     /label="His Tag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     tag             3932..3949
                     /label="Thrombin"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3952..3952
                     /label="Xpress_fwd_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(3952..3952)
                     /label="Tim Excises"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3953..4936
                     /label="pheS CDS (phenylalanine tRNA synthetase, alpha subunit)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            3953..4936
                     /label="pheS gene"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      4944..5072
                     /label="T7_terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4948..4950
                     /label="Stop Codon"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(4986..5004)
                     /label="T7_Terminal_primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            complement(join(5632..6250,1..197))
                     /label="KanR2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
       61 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
      121 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
      181 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
      241 gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      301 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      361 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      421 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
      481 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      541 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      601 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      661 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      721 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      781 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      841 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      901 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      961 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1021 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1081 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     1141 catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     1201 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     1261 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     1321 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     1381 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     1441 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     1501 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     1561 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     1621 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     1681 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     1741 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     1801 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     1861 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     1921 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     1981 tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     2041 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     2101 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     2161 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     2221 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     2281 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     2341 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2401 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     2461 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     2521 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     2581 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     2641 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     2701 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     2761 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     2821 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     2881 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     2941 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     3001 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     3061 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     3121 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     3181 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     3241 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     3301 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     3361 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     3421 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     3481 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     3541 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     3601 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     3661 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     3721 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     3781 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
     3841 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgggcag
     3901 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatgtcaca
     3961 tctcgcagaa ctggttgcca gtgcgaaggc ggccattagc caggcgtcag atgttgccgc
     4021 gttagataat gtgcgcgtcg aatatttggg taaaaaaggg cacttaaccc ttcagatgac
     4081 gaccctgcgt gagctgccgc cagaagagcg tccggcagct ggtgcggtta tcaacgaagc
     4141 gaaagagcag gttcagcagg cgctgaatgc gcgtaaagcg gaactggaaa gcgctgcact
     4201 gaatgcgcgt ctggcggcgg aaacgattga tgtctctctg ccaggtcgtc gcattgaaaa
     4261 cggcggtctg catccggtta cccgtaccat cgaccgtatc gaaagtttct tcggtgagct
     4321 tggctttacc gtggcaaccg ggccggaaat cgaagacgat tatcataact tcgatgctct
     4381 gaacattcct ggtcaccacc cggcgcgcgc tgaccacgac actttctggt ttgacactac
     4441 ccgcctgctg cgtacccaga cctctggcgt acagatccgc accatgaaag cccagcagcc
     4501 accgattcgt atcatcgcgc ctggccgtgt ttatcgtaac gactacgacc agactcacac
     4561 gccgatgttc catcagatgg aaggtctgat tgttgatacc aacatcagct ttaccaacct
     4621 gaaaggcacg ctgcacgact tcctgcgtaa cttctttgag gaagatttgc agattcgctt
     4681 ccgtccttcc tacttcccgt ttaccgaacc ttctgcagaa gtggacgtca tgggtaaaaa
     4741 cggtaaatgg ctggaagtgc tgggctgcgg gatggtgcat ccgaacgtgt tgcgtaacgt
     4801 tggcatcgac ccggaagttt actctggttt cgccttcggg atggggatgg agcgtctgac
     4861 tatgttgcgt tacggcgtca ccgacctgcg ttcattcttc gaaaacgatc tgcgtttcct
     4921 caaacagttt aaataagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
     4981 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     5041 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
     5101 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5161 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5221 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5281 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5341 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5401 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5461 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5521 atattaacgt ttacaatttc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5581 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa
     5641 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     5701 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     5761 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     5821 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
     5881 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
     5941 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
     6001 gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
     6061 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
     6121 tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt
     6181 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
     6241 catctcatct
//