LOCUS       pET-28a_(+)_ProRS       6943 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          56..78
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#5b637e
                     /ApEinfo_fwdcolor=#5b637e
     feature         72..263
                     /label="ROP"
                     /ApEinfo_revcolor=#1ebdac
                     /ApEinfo_fwdcolor=#1ebdac
     feature         738..1013
                     /label="tet(576-851)"
                     /ApEinfo_revcolor=#ba9231
                     /ApEinfo_fwdcolor=#ba9231
     gene            2246..2509
                     /label="tet (300-563)"
                     /ApEinfo_revcolor=#ffe2b9
                     /ApEinfo_fwdcolor=#ffe2b9
     primer          2438..2458
                     /label="pET28+ Reverse Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     promoter        2541..2559
                     /label="T7 Promoter"
                     /ApEinfo_revcolor=#c8498b
                     /ApEinfo_fwdcolor=#c8498b
     regulatory      2559..2586
                     /label="lacI"
                     /ApEinfo_revcolor=#c3dff3
                     /ApEinfo_fwdcolor=#c3dff3
     regulatory      2559..2586
                     /label="lacO"
                     /ApEinfo_revcolor=#fffbaa
                     /ApEinfo_fwdcolor=#fffbaa
     feature         2604..2620
                     /label="T7 Ribosome Binding Site"
                     /ApEinfo_revcolor=#e12222
                     /ApEinfo_fwdcolor=#e12222
     gene            2628..4343
                     /label="Proline-tRNA ligase"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     primer          3438..3466
                     /label="seqFOR2 Proline-tRNA ligase C-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     primer          3951..3974
                     /label="seqFOR1 Proline-tRNA ligase C-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     feature         4344..4364
                     /label="His Tag and Stop Codon"
                     /ApEinfo_revcolor=#ddb34e
                     /ApEinfo_fwdcolor=#ddb34e
     terminator      4372..4500
                     /label="T7 Terminator"
                     /ApEinfo_revcolor=#d24b19
                     /ApEinfo_fwdcolor=#d24b19
     primer          4475..4499
                     /label="pET28+ Forward Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     gene            5060..5875
                     /label="Kan R2"
                     /ApEinfo_revcolor=#359797
                     /ApEinfo_fwdcolor=#359797
     origin          5981..6600
                     /label="pBR322 Origin"
                     /ApEinfo_revcolor=#9faa9a
                     /ApEinfo_fwdcolor=#9faa9a
ORIGIN
        1 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG
       61 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA
      121 AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC
      181 TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG
      241 GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG
      301 GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT
      361 GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG
      421 AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG
      481 GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC
      541 CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC
      601 GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC
      661 TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG
      721 CGCACCCGTG GGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG
      781 GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC
      841 AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT
      901 GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA
      961 GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT
     1021 CGAGATCCCG GTGCCTAATG AGTGAGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC
     1081 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
     1141 AGAGGCGGTT TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA
     1201 CAGCTGATTG CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT
     1261 TTGCCCCAGC AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT
     1321 GTCTTCGGTA TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC
     1381 GGTAATGGCG CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG
     1441 AACGATGCCC TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC
     1501 GCCTTCCCGT TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC
     1561 CAGACGCAGA CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG
     1621 ACCCAATGCG ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT
     1681 ACTGTTGATG GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC
     1741 AGCTTCCACA GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC
     1801 GCGTTGCGCG AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC
     1861 CATCGACACC ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT
     1921 TTGCGACGGC GCGTGCAGGG CCAGACTGGA GGTGGCAACG CCAATCAGCA ACGACTGTTT
     1981 GCCCGCCAGT TGTTGTGCCA CGCGGTTGGG AATGTAATTC AGCTCCGCCA TCGCCGCTTC
     2041 CACTTTTTCC CGCGTTTTCG CAGAAACGTG GCTGGCCTGG TTCACCACGC GGGAAACGGT
     2101 CTGATAAGAG ACACCGGCAT ACTCTGCGAC ATCGTATAAC GTTACTGGTT TCACATTCAC
     2161 CACCCTGAAT TGACTCTCTT CCGGGCGCTA TCATGCCATA CCGCGAAAGG TTTTGCGCCA
     2221 TTCGATGGTG TCCGGGATCT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC
     2281 CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA
     2341 TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC
     2401 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC
     2461 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG
     2521 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA
     2581 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG CGTACTAGCC
     2641 AATACCTGCT CTCCACTCTC AAGGAGACAC CTGCCGACGC CGAGGTGATC AGCCATCAGC
     2701 TGATGCTGCG CGCCGGGATG ATCCGCAAGC TGGCCTCCGG GTTATATACC TGGCTGCCGA
     2761 CCGGCGTGCG CGTTCTGAAA AAAGTCGAAA ACATCGTGCG TGAAGAGATG AACAACGCCG
     2821 GTGCGATCGA GGTGTCGATG CCGGTGGTTC AGCCAGCCGA TTTGTGGCAA GAGAGTGGTC
     2881 GTTGGGAACA GTACGGTCCG GAACTGCTGC GTTTTGTTGA CCGTGGCGAG CGTCCGTTCG
     2941 TACTCGGCCC AACTCATGAA GAAGTTATCA CTGACCTGAT TCGTAACGAG CTTAGCTCTT
     3001 ACAAACAGCT GCCGCTGAAC TTCTATCAGA TCCAGACCAA GTTCCGCGAC GAAGTGCGTC
     3061 CGCGTTTCGG CGTCATGCGT TCCCGCGAAT TCCTGATGAA AGATGCTTAC TCTTTCCATA
     3121 CTTCTCAGGA ATCCCTGCAG GAAACCTACG ATGCAATGTA TGCGGCCTAC AGCAAAATCT
     3181 TCAGCCGCAT GGGGCTGGAT TTCCGCGCCG TACAAGCCGA CACCGGTTCT ATCGGCGGCA
     3241 GCGCCTCTCA CGAATTCCAG GTGCTGGCGC AGAGCGGTGA AGACGATGTG GTCTTCTCCG
     3301 ACACCTCTGA CTATGCAGCG AACATTGAAC TGGCAGAAGC TATCGCGCCG AAAGAACCGC
     3361 GCGCTGCTGC TACCCAGGAA ATGACGCTGG TTGATACGCC GAACGCGAAA ACCATCGCGG
     3421 AACTGGTTGA ACAGTTCAAT CTGCCGATTG AGAAAACGGT TAAGACTCTG CTGGTTAAAG
     3481 CGGTTGAAGG CAGCAGCTTC CCGCAGGTTG CGCTGCTGGT GCGCGGTGAT CACGAGCTGA
     3541 ACGAAGTTAA AGCAGAAAAA CTGCCGCAGG TTGCAAGCCC GCTGACTTTC GCGACCGAAG
     3601 AAGAAATTCG TGCCGTGGTT AAAGCCGGTC CGGGTTCACT GGGTCCGGTA AACATGCCGA
     3661 TTCCGGTGGT GATTGACCGT ACCGTTGCGG CGATGAGTGA TTTCGCTGCT GGTGCTAACA
     3721 TCGATGGTAA ACACTACTTC GGCATCAACT GGGATCGCGA TGTCGCTACC CCGGAAGTTG
     3781 CAGATATCCG TAACGTGGTG GCTGGCGATC CAAGCCCGGA TGGCCAGGGT AGGCTGCTGA
     3841 TCAAACGTGG TATCGAAGTT GGTCACATCT TCCAGCTGGG TACCAAGTAC TCCGAAGCAC
     3901 TGAAAGCCTC CGTACAGGGT GAAGATGGCC GTAACCAAAT CCTGACGATG GGTTGCTACG
     3961 GTATCGGGGT AACGCGTGTG GTAGCTGCGG CGATTGAGCA GAACTACGAC GAACGAGGCA
     4021 TCGTATGGCC TGACGCTATC GCGCCGTTCC AGGTGGCGAT TCTGCCGATG AACATGCACA
     4081 AATCCTTCCG CGTACAAGAG CTTGCTGAGA AACTGTACAG CGAACTGCGT GCACAAGGTA
     4141 TCGAAGTGCT GCTGGATGAC CGCAAAGAGC GTCCGGGCGT GATGTTTGCT GATATGGAAC
     4201 TGATCGGTAT TCCGCACACT ATTGTGCTGG GCGACCGTAA CCTCGACAAC GACGATATCG
     4261 AATATAAATA TCGTCGCAAC GGCGAGAAAC AGTTAATTAA GACTGGTGAC ATCGTCGAAT
     4321 ATCTGGTGAA ACAGATTAAA GGCCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA
     4381 AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC
     4441 TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT
     4501 TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
     4561 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
     4621 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
     4681 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
     4741 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
     4801 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
     4861 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA
     4921 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT
     4981 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA
     5041 TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT
     5101 TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA
     5161 ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC
     5221 GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA
     5281 AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC
     5341 AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC
     5401 CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC
     5461 AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT
     5521 TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG
     5581 TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA
     5641 TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC
     5701 CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG
     5761 TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA
     5821 TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC
     5881 CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA
     5941 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
     6001 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
     6061 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
     6121 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
     6181 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC
     6241 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
     6301 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC
     6361 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA
     6421 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT
     6481 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG
     6541 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG
     6601 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA
     6661 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC
     6721 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG
     6781 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA
     6841 CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG
     6901 GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCG
//