LOCUS       pET-28a_(+)_SerRS       6517 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          56..78
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#5b637e
                     /ApEinfo_fwdcolor=#5b637e
     feature         72..263
                     /label="ROP"
                     /ApEinfo_revcolor=#1ebdac
                     /ApEinfo_fwdcolor=#1ebdac
     feature         738..1013
                     /label="tet(576-851)"
                     /ApEinfo_revcolor=#ba9231
                     /ApEinfo_fwdcolor=#ba9231
     gene            2246..2509
                     /label="tet (300-563)"
                     /ApEinfo_revcolor=#ffe2b9
                     /ApEinfo_fwdcolor=#ffe2b9
     primer          2438..2458
                     /label="pET28+ Reverse Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     promoter        2541..2559
                     /label="T7 Promoter"
                     /ApEinfo_revcolor=#c8498b
                     /ApEinfo_fwdcolor=#c8498b
     regulatory      2559..2586
                     /label="lacI"
                     /ApEinfo_revcolor=#c3dff3
                     /ApEinfo_fwdcolor=#c3dff3
     regulatory      2559..2586
                     /label="lacO"
                     /ApEinfo_revcolor=#fffbaa
                     /ApEinfo_fwdcolor=#fffbaa
     feature         2604..2620
                     /label="T7 Ribosome Binding Site"
                     /ApEinfo_revcolor=#e12222
                     /ApEinfo_fwdcolor=#e12222
     gene            2628..3917
                     /label="Serine-tRNA ligase"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     primer          3007..3030
                     /label="seqFOR2 Serine-tRNA ligase C-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     primer          3529..3548
                     /label="seqFOR1 Serine-tRNA ligase C-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     feature         3918..3938
                     /label="His Tag and Stop Codon"
                     /ApEinfo_revcolor=#ddb34e
                     /ApEinfo_fwdcolor=#ddb34e
     terminator      3946..4074
                     /label="T7 Terminator"
                     /ApEinfo_revcolor=#d24b19
                     /ApEinfo_fwdcolor=#d24b19
     primer          4049..4073
                     /label="pET28+ Forward Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     gene            4634..5449
                     /label="Kan R2"
                     /ApEinfo_revcolor=#359797
                     /ApEinfo_fwdcolor=#359797
     origin          5555..6174
                     /label="pBR322 Origin"
                     /ApEinfo_revcolor=#9faa9a
                     /ApEinfo_fwdcolor=#9faa9a
ORIGIN
        1 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG
       61 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA
      121 AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC
      181 TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG
      241 GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG
      301 GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT
      361 GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG
      421 AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG
      481 GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC
      541 CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC
      601 GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC
      661 TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG
      721 CGCACCCGTG GGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG
      781 GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC
      841 AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT
      901 GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA
      961 GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT
     1021 CGAGATCCCG GTGCCTAATG AGTGAGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC
     1081 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
     1141 AGAGGCGGTT TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA
     1201 CAGCTGATTG CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT
     1261 TTGCCCCAGC AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT
     1321 GTCTTCGGTA TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC
     1381 GGTAATGGCG CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG
     1441 AACGATGCCC TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC
     1501 GCCTTCCCGT TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC
     1561 CAGACGCAGA CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG
     1621 ACCCAATGCG ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT
     1681 ACTGTTGATG GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC
     1741 AGCTTCCACA GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC
     1801 GCGTTGCGCG AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC
     1861 CATCGACACC ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT
     1921 TTGCGACGGC GCGTGCAGGG CCAGACTGGA GGTGGCAACG CCAATCAGCA ACGACTGTTT
     1981 GCCCGCCAGT TGTTGTGCCA CGCGGTTGGG AATGTAATTC AGCTCCGCCA TCGCCGCTTC
     2041 CACTTTTTCC CGCGTTTTCG CAGAAACGTG GCTGGCCTGG TTCACCACGC GGGAAACGGT
     2101 CTGATAAGAG ACACCGGCAT ACTCTGCGAC ATCGTATAAC GTTACTGGTT TCACATTCAC
     2161 CACCCTGAAT TGACTCTCTT CCGGGCGCTA TCATGCCATA CCGCGAAAGG TTTTGCGCCA
     2221 TTCGATGGTG TCCGGGATCT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC
     2281 CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA
     2341 TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC
     2401 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC
     2461 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG
     2521 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA
     2581 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG CTCGATCCCA
     2641 ATCTGCTGCG TAATGAGCCA GACGCAGTCG CTGAAAAACT GGCACGCCGG GGCTTTAAGC
     2701 TGGATGTAGA TAAGCTGGGC GCTCTTGAAG AGCGTCGTAA AGTATTGCAG GTCAAAACGG
     2761 AAAACCTGCA AGCGGAGCGT AACTCCCGAT CGAAATCCAT TGGCCAGGCG AAAGCGCGCG
     2821 GGGAAGATAT CGAGCCTTTA CGTCTGGAAG TGAACAAACT GGGCGAAGAG CTGGATGCAG
     2881 CAAAAGCCGA GCTGGATGCT TTACAGGCTG AAATTCGCGA TATCGCGCTG ACCATCCCTA
     2941 ACCTGCCTGC AGATGAAGTG CCGGTAGGTA AAGACGAAAA TGACAACGTT GAAGTCAGCC
     3001 GCTGGGGTAC CCCGCGTGAG TTTGACTTTG AAGTTCGTGA CCACGTGACG CTGGGTGAAA
     3061 TGCACTCTGG CCTCGACTTT GCAGCTGCAG TTAAGCTGAC TGGTTCCCGC TTTGTGGTAA
     3121 TGAAAGGGCA GATTGCTCGC ATGCACCGCG CACTGTCGCA GTTTATGCTG GATCTGCATA
     3181 CCGAACAGCA TGGCTACAGT GAGAACTATG TTCCGTACCT GGTTAACCAG GACACGCTGT
     3241 ACGGTACGGG TCAACTGCCG AAATTTGCTG GCGATCTGTT CCATACTCGT CCGCTGGAAG
     3301 AAGAAGCAGA CACCAGTAAC TATGCGCTGA TCCCAACGGC AGAAGTTCCG CTGACTAACC
     3361 TGGTGCGCGG TGAAATCATC GATGAAGATG ATCTGCCAAT TAAGATGACC GCCCACACCC
     3421 CATGCTTCCG TTCTGAAGCC GGTTCATATG GTCGTGACAC CCGTGGTCTG ATCCGTATGC
     3481 ACCAGTTCGA CAAAGTTGAA ATGGTGCAGA TCGTGCGCCC AGAAGACTCA ATGGCGGCGC
     3541 TGGAAGAGAT GACTGGTCAT GCAGAAAAAG TCCTGCAGTT GCTGGGCCTG CCGTACCGTA
     3601 AAATCATCCT TTGCACTGGC GACATGGGCT TTGGCGCTTG CAAAACTTAC GACCTGGAAG
     3661 TATGGATCCC GGCACAGAAC ACCTACCGTG AGATCTCTTC CTGCTCCAAC GTTTGGGATT
     3721 TCCAGGCACG TCGTATGCAG GCACGTTGCC GCAGCAAGTC GGACAAGAAA ACCCGTCTGG
     3781 TTCATACCCT GAACGGTTCT GGTCTGGCTG TTGGTCGTAC GCTGGTTGCA GTAATGGAAA
     3841 ACTATCAGCA GGCTGATGGT CGTATTGAAG TACCAGAAGT TCTGCGTCCG TATATGAACG
     3901 GACTGGAATA TATTGGCCAC CACCACCACC ACCACTGAGA TCCGGCTGCT AACAAAGCCC
     3961 GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG
     4021 CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATTGGCGA
     4081 ATGGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT
     4141 GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT
     4201 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG
     4261 ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG
     4321 TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
     4381 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA
     4441 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA
     4501 ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TCAGGTGGCA CTTTTCGGGG
     4561 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
     4621 CATGAATTAA TTCTTAGAAA AACTCATCGA GCATCAAATG AAACTGCAAT TTATTCATAT
     4681 CAGGATTATC AATACCATAT TTTTGAAAAA GCCGTTTCTG TAATGAAGGA GAAAACTCAC
     4741 CGAGGCAGTT CCATAGGATG GCAAGATCCT GGTATCGGTC TGCGATTCCG ACTCGTCCAA
     4801 CATCAATACA ACCTATTAAT TTCCCCTCGT CAAAAATAAG GTTATCAAGT GAGAAATCAC
     4861 CATGAGTGAC GACTGAATCC GGTGAGAATG GCAAAAGTTT ATGCATTTCT TTCCAGACTT
     4921 GTTCAACAGG CCAGCCATTA CGCTCGTCAT CAAAATCACT CGCATCAACC AAACCGTTAT
     4981 TCATTCGTGA TTGCGCCTGA GCGAGACGAA ATACGCGATC GCTGTTAAAA GGACAATTAC
     5041 AAACAGGAAT CGAATGCAAC CGGCGCAGGA ACACTGCCAG CGCATCAACA ATATTTTCAC
     5101 CTGAATCAGG ATATTCTTCT AATACCTGGA ATGCTGTTTT CCCGGGGATC GCAGTGGTGA
     5161 GTAACCATGC ATCATCAGGA GTACGGATAA AATGCTTGAT GGTCGGAAGA GGCATAAATT
     5221 CCGTCAGCCA GTTTAGTCTG ACCATCTCAT CTGTAACATC ATTGGCAACG CTACCTTTGC
     5281 CATGTTTCAG AAACAACTCT GGCGCATCGG GCTTCCCATA CAATCGATAG ATTGTCGCAC
     5341 CTGATTGCCC GACATTATCG CGAGCCCATT TATACCCATA TAAATCAGCA TCCATGTTGG
     5401 AATTTAATCG CGGCCTAGAG CAAGACGTTT CCCGTTGAAT ATGGCTCATA ACACCCCTTG
     5461 TATTACTGTT TATGTAAGCA GACAGTTTTA TTGTTCATGA CCAAAATCCC TTAACGTGAG
     5521 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT
     5581 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT
     5641 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG
     5701 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT
     5761 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC
     5821 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG
     5881 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA
     5941 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
     6001 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG
     6061 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA
     6121 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT
     6181 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT
     6241 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA
     6301 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT
     6361 CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT
     6421 GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT
     6481 GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCG
//