LOCUS       pET-28a_(+)_ThrRS       7195 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          58..80
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#5b637e
                     /ApEinfo_fwdcolor=#5b637e
     feature         74..265
                     /label="ROP"
                     /ApEinfo_revcolor=#1ebdac
                     /ApEinfo_fwdcolor=#1ebdac
     feature         740..1015
                     /label="tet(576-851)"
                     /ApEinfo_revcolor=#ba9231
                     /ApEinfo_fwdcolor=#ba9231
     gene            2248..2511
                     /label="tet (300-563)"
                     /ApEinfo_revcolor=#ffe2b9
                     /ApEinfo_fwdcolor=#ffe2b9
     primer          2440..2460
                     /label="pET28+ Reverse Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     promoter        2543..2561
                     /label="T7 Promoter"
                     /ApEinfo_revcolor=#c8498b
                     /ApEinfo_fwdcolor=#c8498b
     regulatory      2561..2588
                     /label="lacO"
                     /ApEinfo_revcolor=#fffbaa
                     /ApEinfo_fwdcolor=#fffbaa
     regulatory      2561..2588
                     /label="lacI"
                     /ApEinfo_revcolor=#c3dff3
                     /ApEinfo_fwdcolor=#c3dff3
     feature         2606..2622
                     /label="T7 Ribosome Binding Site"
                     /ApEinfo_revcolor=#e12222
                     /ApEinfo_fwdcolor=#e12222
     tag             2642..2659
                     /label="His Tag"
                     /ApEinfo_revcolor=#f9c5fe
                     /ApEinfo_fwdcolor=#f9c5fe
     tag             2669..2686
                     /label="thrombin"
                     /ApEinfo_revcolor=#5f631d
                     /ApEinfo_fwdcolor=#5f631d
     gene            2690..4618
                     /label="Threonine-tRNA ligase"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     primer          3175..3198
                     /label="seqFOR3 Threonine-tRNA ligase N-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     primer          3692..3717
                     /label="seqFOR2 Threonine-tRNA ligase N-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     primer          4210..4235
                     /label="seqFOR1 Threonine-tRNA ligase N-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     terminator      4626..4754
                     /label="T7 Terminator"
                     /ApEinfo_revcolor=#d24b19
                     /ApEinfo_fwdcolor=#d24b19
     primer          4729..4753
                     /label="pET28+ Forward Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     gene            5314..6129
                     /label="Kan R2"
                     /ApEinfo_revcolor=#359797
                     /ApEinfo_fwdcolor=#359797
     origin          6235..6854
                     /label="pBR322 Origin"
                     /ApEinfo_revcolor=#9faa9a
                     /ApEinfo_fwdcolor=#9faa9a
ORIGIN
        1 CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG
       61 GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT
      121 AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA
      181 GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA
      241 GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG
      301 GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC
      361 ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC
      421 AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC
      481 AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT
      541 TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG
      601 TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT
      661 GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA
      721 TGCGCACCCG TGGGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG
      781 TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG
      841 ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG
      901 CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA
      961 TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG
     1021 GTCGAGATCC CGGTGCCTAA TGAGTGAGCT AACTTACATT AATTGCGTTG CGCTCACTGC
     1081 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG
     1141 GGAGAGGCGG TTTGCGTATT GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC
     1201 AACAGCTGAT TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG
     1261 GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTA ACGGCGGGAT ATAACATGAG
     1321 CTGTCTTCGG TATCGTCGTA TCCCACTACC GAGATATCCG CACCAACGCG CAGCCCGGAC
     1381 TCGGTAATGG CGCGCATTGC GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG
     1441 GGAACGATGC CCTCATTCAG CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG
     1501 TCGCCTTCCC GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA
     1561 GCCAGACGCA GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC GATTTGCTGG
     1621 TGACCCAATG CGACCAGATG CTCCACGCCC AGTCGCGTAC CGTCTTCATG GGAGAAAATA
     1681 ATACTGTTGA TGGGTGTCTG GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG
     1741 GCAGCTTCCA CAGCAATGGC ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG
     1801 ACGCGTTGCG CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT
     1861 ACCATCGACA CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT CGCCGCGACA
     1921 ATTTGCGACG GCGCGTGCAG GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT
     1981 TTGCCCGCCA GTTGTTGTGC CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT
     2041 TCCACTTTTT CCCGCGTTTT CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG
     2101 GTCTGATAAG AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC
     2161 ACCACCCTGA ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA GGTTTTGCGC
     2221 CATTCGATGG TGTCCGGGAT CTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA
     2281 GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA
     2341 GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC
     2401 GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC
     2461 GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT
     2521 CGAGATCTCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC
     2581 AATTCCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGGCAGCAG
     2641 CCATCATCAT CATCATCACA GCAGCGGCCT GGTGCCGCGC GGCAGCCATA TGCCTGTTAT
     2701 AACTCTTCCT GATGGCAGCC AACGCCATTA CGATCACGCT GTAAGCCCCA TGGATGTTGC
     2761 GCTGGACATT GGTCCAGGTC TGGCGAAAGC CTGTATCGCA GGGCGCGTTA ATGGCGAACT
     2821 GGTTGATGCT TGCGATCTGA TTGAAAACGA CGCACAACTG TCGATCATTA CCGCCAAAGA
     2881 CGAAGAAGGT CTGGAGATCA TTCGTCACTC CTGTGCGCAC CTGTTAGGGC ACGCGATTAA
     2941 ACAACTTTGG CCGCATACCA AAATGGCAAT CGGCCCGGTT ATTGACAACG GTTTTTATTA
     3001 CGACGTTGAT CTTGACCGCA CGTTAACCCA GGAAGATGTC GAAGCACTCG AGAAGCGGAT
     3061 GCATGAGCTT GCTGAGAAAA ACTACGACGT CATTAAGAAG AAAGTCAGCT GGCACGAAGC
     3121 GCGTGAAACT TTCGCCAACC GTGGGGAGAG CTACAAAGTC TCCATTCTTG ACGAAAACAT
     3181 CGCCCATGAT GACAAGCCAG GTCTGTACTT CCATGAAGAA TATGTCGATA TGTGCCGCGG
     3241 TCCGCACGTA CCGAACATGC GTTTCTGCCA TCATTTCAAA CTAATGAAAA CGGCAGGGGC
     3301 TTACTGGCGT GGCGACAGCA ACAACAAAAT GTTGCAACGT ATTTACGGTA CGGCGTGGGC
     3361 AGACAAAAAA GCACTTAACG CTTACCTGCA GCGCCTGGAA GAAGCCGCGA AACGCGACCA
     3421 CCGTAAAATC GGTAAACAGC TCGACCTGTA CCATATGCAG GAAGAAGCGC CGGGTATGGT
     3481 ATTCTGGCAC AACGACGGCT GGACCATCTT CCGTGAACTG GAAGTGTTTG TTCGTTCTAA
     3541 ACTGAAAGAG TACCAGTATC AGGAAGTTAA AGGTCCGTTC ATGATGGACC GTGTCCTGTG
     3601 GGAAAAAACC GGTCACTGGG ACAACTACAA AGATGCAATG TTCACCACAT CTTCTGAGAA
     3661 CCGTGAATAC TGCATTAAGC CGATGAACTG CCCGGGTCAC GTACAAATTT TCAACCAGGG
     3721 GCTGAAGTCT TATCGCGATC TGCCGCTGCG TATGGCCGAG TTTGGTAGCT GCCACCGTAA
     3781 CGAGCCGTCA GGTTCGCTGC ATGGCCTGAT GCGCGTGCGT GGATTTACCC AGGATGACGC
     3841 GCATATCTTC TGTACTGAAG AACAAATTCG CGATGAAGTT AACGGATGTA TCCGTTTAGT
     3901 CTATGATATG TACAGCACTT TTGGCTTCGA GAAGATCGTC GTCAAACTCT CCACTCGTCC
     3961 TGAAAAACGT ATTGGCAGCG ACGAAATGTG GGATCGTGCT GAGGCGGACC TGGCGGTTGC
     4021 GCTGGAAGAA AACAACATCC CGTTTGAATA TCAACTGGGT GAAGGCGCTT TCTACGGTCC
     4081 GAAAATTGAA TTTACCCTGT ATGACTGCCT CGATCGTGCA TGGCAGTGCG GTACAGTACA
     4141 GCTGGACTTC TCTTTGCCGT CTCGTCTGAG CGCTTCTTAT GTAGGCGAAG ACAATGAACG
     4201 TAAAGTACCG GTAATGATTC ACCGCGCAAT TCTGGGGTCG ATGGAACGTT TCATCGGTAT
     4261 CCTGACCGAA GAGTTCGCTG GTTTCTTCCC GACCTGGCTT GCGCCGGTTC AGGTTGTTAT
     4321 CATGAATATT ACCGATTCAC AGTCTGAATA CGTTAACGAA TTGACGCAAA AACTATCAAA
     4381 TGCGGGCATT CGTGTTAAAG CAGACTTGAG AAATGAGAAG ATTGGCTTTA AAATCCGCGA
     4441 GCACACTTTG CGTCGCGTCC CATATATGCT GGTCTGTGGT GATAAAGAGG TGGAATCAGG
     4501 CAAAGTTGCC GTTCGCACCC GCCGTGGTAA AGACCTGGGA AGCATGGACG TAAATGAAGT
     4561 GATCGAGAAG CTGCAACAAG AGATTCGCAG CCGCAGTCTT AAACAATTGG AGGAATAAGA
     4621 TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA
     4681 ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG
     4741 AACTATATCC GGATTGGCGA ATGGGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT
     4801 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC
     4861 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG
     4921 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT
     4981 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG
     5041 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT
     5101 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
     5161 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT
     5221 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA
     5281 CATTCAAATA TGTATCCGCT CATGAATTAA TTCTTAGAAA AACTCATCGA GCATCAAATG
     5341 AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT TTTTGAAAAA GCCGTTTCTG
     5401 TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG GCAAGATCCT GGTATCGGTC
     5461 TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT TTCCCCTCGT CAAAAATAAG
     5521 GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC GGTGAGAATG GCAAAAGTTT
     5581 ATGCATTTCT TTCCAGACTT GTTCAACAGG CCAGCCATTA CGCTCGTCAT CAAAATCACT
     5641 CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA GCGAGACGAA ATACGCGATC
     5701 GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC CGGCGCAGGA ACACTGCCAG
     5761 CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT AATACCTGGA ATGCTGTTTT
     5821 CCCGGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA GTACGGATAA AATGCTTGAT
     5881 GGTCGGAAGA GGCATAAATT CCGTCAGCCA GTTTAGTCTG ACCATCTCAT CTGTAACATC
     5941 ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT GGCGCATCGG GCTTCCCATA
     6001 CAATCGATAG ATTGTCGCAC CTGATTGCCC GACATTATCG CGAGCCCATT TATACCCATA
     6061 TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTAGAG CAAGACGTTT CCCGTTGAAT
     6121 ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA GACAGTTTTA TTGTTCATGA
     6181 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     6241 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     6301 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     6361 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
     6421 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     6481 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     6541 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     6601 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
     6661 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     6721 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     6781 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     6841 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
     6901 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
     6961 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
     7021 AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG
     7081 GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA
     7141 TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACG
//