LOCUS       pET-28a_(+)_TyrRS       6499 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          56..78
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#5b637e
                     /ApEinfo_fwdcolor=#5b637e
     feature         72..263
                     /label="ROP"
                     /ApEinfo_revcolor=#1ebdac
                     /ApEinfo_fwdcolor=#1ebdac
     feature         738..1013
                     /label="tet(576-851)"
                     /ApEinfo_revcolor=#ba9231
                     /ApEinfo_fwdcolor=#ba9231
     gene            2246..2509
                     /label="tet (300-563)"
                     /ApEinfo_revcolor=#ffe2b9
                     /ApEinfo_fwdcolor=#ffe2b9
     primer          2438..2458
                     /label="pET28+ Reverse Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     promoter        2541..2559
                     /label="T7 Promoter"
                     /ApEinfo_revcolor=#c8498b
                     /ApEinfo_fwdcolor=#c8498b
     regulatory      2559..2586
                     /label="lacI"
                     /ApEinfo_revcolor=#c3dff3
                     /ApEinfo_fwdcolor=#c3dff3
     regulatory      2559..2586
                     /label="lacO"
                     /ApEinfo_revcolor=#fffbaa
                     /ApEinfo_fwdcolor=#fffbaa
     feature         2604..2620
                     /label="T7 Ribosome Binding Site"
                     /ApEinfo_revcolor=#e12222
                     /ApEinfo_fwdcolor=#e12222
     gene            2628..3899
                     /label="Tyrosine-tRNA ligase"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     primer          3000..3030
                     /label="seqFOR2 Tyrosine-tRNA ligase C-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     primer          3526..3548
                     /label="seqFOR1 Tyrosine-tRNA ligase C-tagged"
                     /ApEinfo_revcolor=#f2c22e
                     /ApEinfo_fwdcolor=#f2c22e
     feature         3900..3920
                     /label="His Tag and Stop Codon"
                     /ApEinfo_revcolor=#ddb34e
                     /ApEinfo_fwdcolor=#ddb34e
     terminator      3928..4056
                     /label="T7 Terminator"
                     /ApEinfo_revcolor=#d24b19
                     /ApEinfo_fwdcolor=#d24b19
     primer          4031..4055
                     /label="pET28+ Forward Primer"
                     /ApEinfo_revcolor=#fffafa
                     /ApEinfo_fwdcolor=#fffafa
     gene            4616..5431
                     /label="Kan R2"
                     /ApEinfo_revcolor=#359797
                     /ApEinfo_fwdcolor=#359797
     origin          5537..6156
                     /label="pBR322 Origin"
                     /ApEinfo_revcolor=#9faa9a
                     /ApEinfo_fwdcolor=#9faa9a
ORIGIN
        1 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG
       61 AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA
      121 AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC
      181 TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG
      241 GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG
      301 GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT
      361 GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG
      421 AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG
      481 GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC
      541 CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC
      601 GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC
      661 TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG
      721 CGCACCCGTG GGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG
      781 GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC
      841 AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT
      901 GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA
      961 GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT
     1021 CGAGATCCCG GTGCCTAATG AGTGAGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC
     1081 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
     1141 AGAGGCGGTT TGCGTATTGG GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA
     1201 CAGCTGATTG CCCTTCACCG CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT
     1261 TTGCCCCAGC AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT
     1321 GTCTTCGGTA TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC
     1381 GGTAATGGCG CGCATTGCGC CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG
     1441 AACGATGCCC TCATTCAGCA TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC
     1501 GCCTTCCCGT TCCGCTATCG GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC
     1561 CAGACGCAGA CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG
     1621 ACCCAATGCG ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT
     1681 ACTGTTGATG GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC
     1741 AGCTTCCACA GCAATGGCAT CCTGGTCATC CAGCGGATAG TTAATGATCA GCCCACTGAC
     1801 GCGTTGCGCG AGAAGATTGT GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC
     1861 CATCGACACC ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT
     1921 TTGCGACGGC GCGTGCAGGG CCAGACTGGA GGTGGCAACG CCAATCAGCA ACGACTGTTT
     1981 GCCCGCCAGT TGTTGTGCCA CGCGGTTGGG AATGTAATTC AGCTCCGCCA TCGCCGCTTC
     2041 CACTTTTTCC CGCGTTTTCG CAGAAACGTG GCTGGCCTGG TTCACCACGC GGGAAACGGT
     2101 CTGATAAGAG ACACCGGCAT ACTCTGCGAC ATCGTATAAC GTTACTGGTT TCACATTCAC
     2161 CACCCTGAAT TGACTCTCTT CCGGGCGCTA TCATGCCATA CCGCGAAAGG TTTTGCGCCA
     2221 TTCGATGGTG TCCGGGATCT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC
     2281 CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA
     2341 TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC
     2401 TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC
     2461 CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG
     2521 AGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA
     2581 TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG GCAAGCAGTA
     2641 ACTTGATTAA ACAATTGCAA GAGCGGGGGC TGGTAGCCCA GGTGACGGAC GAGGAAGCGT
     2701 TAGCAGAGCG ACTGGCGCAA GGCCCGATCG CGCTCTATTG CGGCTTCGAT CCTACCGCTG
     2761 ACAGCTTGCA TTTGGGGCAT CTTGTTCCAT TGTTATGCCT GAAACGCTTC CAGCAGGCGG
     2821 GCCACAAGCC GGTTGCGCTG GTAGGCGGCG CGACGGGTCT GATTGGCGAC CCGAGCTTCA
     2881 AAGCTGCCGA GCGTAAGCTG AACACCGAAG AAACTGTTCA GGAGTGGGTG GACAAAATCC
     2941 GTAAGCAGGT TGCCCCGTTC CTCGATTTCG ACTGTGGAGA AAACTCTGCT ATCGCGGCGA
     3001 ACAACTATGA CTGGTTCGGC AATATGAATG TGCTGACCTT CCTGCGCGAT ATTGGCAAAC
     3061 ACTTCTCCGT TAACCAGATG ATCAACAAAG AAGCGGTTAA GCAGCGTCTC AACCGTGAAG
     3121 ATCAGGGGAT TTCGTTCACT GAGTTTTCCT ACAACCTGTT GCAGGGTTAT GACTTCGCCT
     3181 GTCTGAACAA ACAGTACGGT GTGGTGCTGC AAATTGGTGG TTCTGACCAG TGGGGTAACA
     3241 TCACTTCTGG TATCGACCTG ACCCGTCGTC TGCATCAGAA TCAGGTGTTT GGCCTGACCG
     3301 TTCCGCTGAT CACTAAAGCA GATGGCACCA AATTTGGTAA AACTGAAGGC GGCGCAGTCT
     3361 GGTTGGATCC GAAGAAAACC AGCCCGTACA AATTCTACCA GTTCTGGATC AACACTGCGG
     3421 ATGCCGACGT TTACCGCTTC CTGAAGTTCT TCACCTTTAT GAGCATTGAA GAGATCAACG
     3481 CCCTGGAAGA AGAAGATAAA AACAGCGGTA AAGCACCGCG CGCCCAGTAT GTACTGGCGG
     3541 AGCAGGTGAC TCGTCTGGTT CACGGTGAAG AAGGTTTACA GGCGGCAAAA CGTATTACCG
     3601 AATGCCTGTT CAGCGGTTCT TTGAGTGCGC TGAGTGAAGC GGACTTCGAA CAGCTGGCGC
     3661 AGGACGGCGT ACCGATGGTT GAGATGGAAA AGGGCGCAGA CCTGATGCAG GCACTGGTCG
     3721 ATTCTGAACT GCAACCTTCC CGTGGTCAGG CACGTAAAAC TATCGCCTCC AATGCCATCA
     3781 CCATTAACGG TGAAAAACAG TCCGATCCTG AATACTTCTT TAAAGAAGAA GATCGTCTGT
     3841 TTGGTCGTTT TACCTTACTG CGTCGCGGTA AAAAGAATTA CTGTCTGATT TGCTGGAAAC
     3901 ACCACCACCA CCACCACTGA GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG
     3961 CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA
     4021 GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATTGGC GAATGGGACG CGCCCTGTAG
     4081 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG
     4141 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT
     4201 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA
     4261 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA
     4321 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA
     4381 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC
     4441 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA
     4501 CAAAATATTA ACGTTTACAA TTTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC
     4561 TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAATT AATTCTTAGA
     4621 AAAACTCATC GAGCATCAAA TGAAACTGCA ATTTATTCAT ATCAGGATTA TCAATACCAT
     4681 ATTTTTGAAA AAGCCGTTTC TGTAATGAAG GAGAAAACTC ACCGAGGCAG TTCCATAGGA
     4741 TGGCAAGATC CTGGTATCGG TCTGCGATTC CGACTCGTCC AACATCAATA CAACCTATTA
     4801 ATTTCCCCTC GTCAAAAATA AGGTTATCAA GTGAGAAATC ACCATGAGTG ACGACTGAAT
     4861 CCGGTGAGAA TGGCAAAAGT TTATGCATTT CTTTCCAGAC TTGTTCAACA GGCCAGCCAT
     4921 TACGCTCGTC ATCAAAATCA CTCGCATCAA CCAAACCGTT ATTCATTCGT GATTGCGCCT
     4981 GAGCGAGACG AAATACGCGA TCGCTGTTAA AAGGACAATT ACAAACAGGA ATCGAATGCA
     5041 ACCGGCGCAG GAACACTGCC AGCGCATCAA CAATATTTTC ACCTGAATCA GGATATTCTT
     5101 CTAATACCTG GAATGCTGTT TTCCCGGGGA TCGCAGTGGT GAGTAACCAT GCATCATCAG
     5161 GAGTACGGAT AAAATGCTTG ATGGTCGGAA GAGGCATAAA TTCCGTCAGC CAGTTTAGTC
     5221 TGACCATCTC ATCTGTAACA TCATTGGCAA CGCTACCTTT GCCATGTTTC AGAAACAACT
     5281 CTGGCGCATC GGGCTTCCCA TACAATCGAT AGATTGTCGC ACCTGATTGC CCGACATTAT
     5341 CGCGAGCCCA TTTATACCCA TATAAATCAG CATCCATGTT GGAATTTAAT CGCGGCCTAG
     5401 AGCAAGACGT TTCCCGTTGA ATATGGCTCA TAACACCCCT TGTATTACTG TTTATGTAAG
     5461 CAGACAGTTT TATTGTTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG
     5521 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC
     5581 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG
     5641 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC
     5701 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC
     5761 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC
     5821 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT
     5881 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT
     5941 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC
     6001 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT
     6061 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA
     6121 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
     6181 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
     6241 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
     6301 TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC
     6361 GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT
     6421 TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC
     6481 GCCAACACCC GCTGACGCG
//