LOCUS       pET-28a_(+)_ValRS       8080 bp ds-DNA     circular     23-MAR-2021
DEFINITION  .
KEYWORDS    "accession:addgene_vdb_2565"                                       
FEATURES             Location/Qualifiers
     primer          56..78
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     feature         complement(72..263)
                     /label="ROP"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            complement(738..1013)
                     /label="tet (576 - 851)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     regulatory      complement(1072..2163)
                     /label="lacI"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            complement(2246..2509)
                     /label="tet (300 - 563)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          2438..2457
                     /label="pBRrevBam_primer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        2541..2559
                     /label="T7_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     regulatory      2559..2586
                     /label="lacO"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     feature         2604..2620
                     /label="T7_transl_en_RBS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2612..2627)
                     /label="Tentative pET28 anneal FOR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             2628..5480
                     /label="Translation 2628-5480"
     CDS             complement(2628..5480)
                     /label="Translation 5480-2628"
     misc_feature    complement(2628..5480)
                     /label="ValS"
     misc_feature    5481..5499
                     /label="pET28 anneal REV"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             5481..5501
                     /label="Translation 5481-5501"
     CDS             5481..5515
                     /label="Translation 5481-5515"
     terminator      5509..5637
                     /label="T7_terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5513..5515
                     /label="Stop Codon"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(5551..5569)
                     /label="T7_Terminal_primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            complement(6197..7012)
                     /label="KanR2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     origin          7118..7737
                     /label="pBR322_origin"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
       61 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
      121 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
      181 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
      241 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
      301 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
      361 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
      421 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
      481 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
      541 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
      601 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
      661 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
      721 cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
      781 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
      841 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
      901 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
      961 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     1021 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
     1081 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1141 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
     1201 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     1261 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     1321 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     1381 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     1441 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     1501 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     1561 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     1621 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
     1681 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     1741 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     1801 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     1861 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     1921 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     1981 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     2041 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     2101 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     2161 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
     2221 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
     2281 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
     2341 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     2401 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     2461 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
     2521 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
     2581 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccATG GAAAAGACAT
     2641 ATAACCCACA AGATATCGAA CAGCCGCTTT ACGAGCACTG GGAAAAGCAG GGCTACTTTA
     2701 AGCCTAATGG CGATGAAAGC CAGGAAAGTT TCTGCATCAT GATCCCGCCG CCGAACGTCA
     2761 CCGGCAGTTT GCATATGGGT CACGCCTTCC AGCAAACCAT CATGGATACC ATGATCCGCT
     2821 ATCAGCGCAT GCAGGGCAAA AACACCCTGT GGCAGGTCGG TACTGACCAC GCCGGGATCG
     2881 CTACCCAGAT GGTCGTTGAG CGCAAGATTG CCGCAGAAGA AGGTAAAACC CGTCACGACT
     2941 ACGGCCGCGA AGCTTTCATC GACAAAATCT GGGAATGGAA AGCGGAATCT GGCGGCACCA
     3001 TTACCCGTCA GATGCGCCGT CTCGGCAACT CCGTCGACTG GGAGCGTGAA CGCTTCACCA
     3061 TGGACGAAGG CCTGTCCAAT GCGGTGAAAG AAGTTTTCGT TCGTCTGTAT AAAGAAGACC
     3121 TGATTTACCG TGGCAAACGC CTGGTAAACT GGGATCCGAA ACTGCGCACC GCTATCTCTG
     3181 ACCTGGAAGT CGAAAACCGC GAATCGAAAG GTTCGATGTG GCACATCCGC TATCCGCTGG
     3241 CTGACGGTGC GAAAACCGCA GACGGTAAAG ATTATCTGGT GGTCGCGACT ACCCGTCCAG
     3301 AAACCCTGCT GGGCGATACT GGCGTAGCCG TTAACCCGGA AGATCCGCGT TACAAAGATC
     3361 TGATTGGCAA ATATGTCATT CTGCCGCTGG TTAACCGTCG TATTCCGATC GTTGGCGACG
     3421 AACACGCCGA CATGGAAAAA GGCACCGGCT GCGTGAAAAT CACTCCGGCG CACGACTTTA
     3481 ACGACTATGA AGTGGGTAAA CGTCACGCCC TGCCGATGAT CAACATCCTG ACCTTTGACG
     3541 GCGATATCCG TGAAAGCGCC CAGGTGTTCG ATACCAAAGG TAACGAATCT GACGTTTATT
     3601 CCAGCGAAAT CCCTGCAGAG TTCCAGAAAC TGGAGCGTTT TGCTGCACGT AAAGCAGTCG
     3661 TTGCCGCAGT TGACGCGCTT GGCCTGCTGG AAGAAATTAA ACCGCACGAC CTGACCGTTC
     3721 CTTACGGCGA CCGTGGCGGC GTAGTTATCG AACCAATGCT GACCGACCAG TGGTACGTGC
     3781 GTGCCGATGT CCTGGCGAAA CCGGCGGTTG AAGCGGTTGA GAACGGCGAC ATTCAGTTCG
     3841 TACCGAAGCA GTACGAAAAC ATGTACTTCT CCTGGATGCG CGATATTCAG GACTGGTGTA
     3901 TCTCTCGTCA GTTGTGGTGG GGTCACCGTA TCCCGGCATG GTATGACGAA GCGGGTAACG
     3961 TTTATGTTGG CCGCAACGAA GACGAAGTGC GTAAAGAAAA TAACCTCGGT GCTGATGTTG
     4021 TCCTGCGTCA GGACGAAGAC GTTCTCGATA CCTGGTTCTC TTCTGCGCTG TGGACCTTCT
     4081 CTACCCTTGG CTGGCCGGAA AATACCGACG CCCTGCGTCA GTTCCACCCA ACCAGCGTGA
     4141 TGGTATCTGG TTTCGACATC ATTTTCTTCT GGATTGCCCG CATGATCATG ATGACCATGC
     4201 ACTTCATCAA AGATGAAAAT GGCAAACCGC AGGTGCCGTT CCACACCGTT TACATGACCG
     4261 GCCTGATTCG TGATGACGAA GGCCAGAAGA TGTCCAAATC CAAGGGTAAC GTTATCGACC
     4321 CACTGGATAT GGTTGACGGT ATTTCGCTGC CAGAACTGCT GGAAAAACGT ACCGGCAATA
     4381 TGATGCAGCC GCAGCTGGCG GACAAAATCC GTAAGCGCAC CGAGAAGCAG TTCCCGAACG
     4441 GTATTGAGCC GCACGGTACT GACGCGCTGC GCTTCACCCT GGCGGCGCTG GCGTCTACCG
     4501 GTCGTGACAT CAACTGGGAT ATGAAGCGTC TGGAAGGTTA CCGTAACTTC TGTAACAAGC
     4561 TGTGGAACGC CAGCCGCTTT GTGCTGATGA ACACAGAAGG TCAGGATTGC GGCTTCAACG
     4621 GCGGCGAAAT GACGCTGTCG CTGGCGGACC GCTGGATTCT GGCGGAGTTC AACCAGACCA
     4681 TCAAAGCGTA CCGCGAAGCG CTGGACAGCT TCCGCTTCGA TATCGCCGCA GGCATTCTGT
     4741 ATGAGTTCAC CTGGAACCAG TTCTGTGACT GGTATCTCGA GCTGACCAAG CCGGTAATGA
     4801 ACGGTGGCAC CGAAGCAGAA CTGCGCGGTA CTCGCCATAC GCTGGTGACT GTACTGGAAG
     4861 GTCTGCTGCG CCTCGCGCAT CCGATCATTC CGTTCATCAC CGAAACCATC TGGCAGCGTG
     4921 TGAAAGTACT TTGCGGTATC ACTGCCGACA CCATCATGCT GCAGCCGTTC CCGCAGTACG
     4981 ATGCATCTCA GGTTGATGAA GCCGCACTGG CCGACACCGA ATGGCTGAAA CAGGCGATCG
     5041 TTGCGGTACG TAACATCCGT GCAGAAATGA ACATCGCGCC GGGCAAACCG CTGGAGCTGC
     5101 TGCTGCGTGG TTGCAGCGCG GATGCAGAAC GTCGCGTAAA TGAAAACCGT GGCTTCCTGC
     5161 AAACCCTGGC GCGTCTGGAA AGTATCACCG TGCTGCCTGC CGATGACAAA GGTCCGGTTT
     5221 CCGTTACGAA GATCATCGAC GGTGCAGAGC TGCTGATCCC GATGGCTGGC CTCATCAACA
     5281 AAGAAGATGA GCTGGCGCGT CTGGCGAAAG AAGTGGCGAA GATTGAAGGT GAAATCAGCC
     5341 GTATCGAGAA CAAACTGGCG AACGAAGGCT TTGTCGCCCG CGCACCGGAA GCGGTCATCG
     5401 CGAAAGAGCG TGAGAAGCTG GAAGGCTATG CGGAAGCGAA AGCGAAACTG ATTGAACAGC
     5461 AGGCTGTTAT CGCCGCGCTG caccaccacc accaccactg agatccggct gctaacaaag
     5521 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     5581 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
     5641 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5701 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5761 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5821 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5881 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5941 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6001 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6061 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcaggtg gcacttttcg
     6121 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6181 gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     6241 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     6301 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     6361 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     6421 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     6481 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     6541 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     6601 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     6661 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     6721 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     6781 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     6841 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     6901 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     6961 tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc
     7021 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgaccaaaat cccttaacgt
     7081 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7141 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7201 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7261 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7321 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7381 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7441 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7501 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7561 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7621 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7681 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7741 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7801 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7861 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     7921 tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa
     7981 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     8041 catggctgcg ccccgacacc cgccaacacc cgctgacgcg
//