LOCUS       BBF10K_000590           6020 bp ds-DNA     circular     10-AUG-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     source          1..4388
                     /label="source"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      38..85
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    265..515
                     /label="incC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(518..1273)
                     /label="repE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     exon            complement(518..1273)
                     /label="Translation 1273-518"
     rep_origin      1364..1583
                     /label="ori2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    2003..2024
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        2039..2069
                     /label="lac promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    2077..2093
                     /label="lac operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     2101..2117
                     /label="M13 rev"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2190..3035
                     /label="repL"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     exon            2190..3035
                     /label="Translation 2190-3035"
     misc_feature    3143..3294
                     /label="nnpp promoters"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3334..4149
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            3334..4149
                     /label="Translation 3334-4149"
     terminator      complement(4259..4302)
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     4320..4336
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      4337..4368
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4389..4408
                     /label="FG Dialout primer FWD"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4409..4414
                     /label="BamHI RE site"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4417..4422
                     /label="BsaI Recognition site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4424..4427
                     /label="BsaI Cut site/MoClo overhang C"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4428..5912
                     /label="yD1E CDS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5913..5915
                     /label="STOP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5918..5921
                     /label="MoClo overhang D"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5922..5925
                     /label="BsaI cut site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5927..5932
                     /label="BasI Recognition Site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5935..5940
                     /label="SalI RE site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5941..5960
                     /label="FG Dialout primer REV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5961..6020
                     /label="FreeGenes Barcode"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5982..5999
                     /label="I-SceI RE site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gtcgcgtatg catcgatcac gtgtccacgt tctttaatag tggactcttg ttccaaactg
      181 gaacaacact cggatcgatc cggcgcgcac cgtgggaaaa actccaggta gaggtacaca
      241 cgcggatagc caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa
      301 ctaacccccg atatcaggtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa
      361 ctgccctcaa atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc
      421 aggctgaagg aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga
      481 cgaaccaccc tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga
      541 tatcgcggaa ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg
      601 agaggcgcat tggagttctg ctgttgatct cattaacaca gacttgcagg aagcggcggc
      661 ggaagtcagg catacgctgg taactttgag gcagctggta acgctctatg atccagtcga
      721 ttttcagaga cacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata
      781 aacgcatggc atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc
      841 ggttctgtaa cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc
      901 ctctggatgg actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat
      961 caccggcatc ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg
     1021 cctgccgtat atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag
     1081 caacatggat ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct
     1141 ggtcaacgaa cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag
     1201 tgaggtcgtt tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg
     1261 ctgtttccgc catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa
     1321 taaaaaagag tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat
     1381 ttgtcacagg gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc
     1441 acactgaaag ggcaatttgt cacaacacct tctgtagaac cagcatggat aaaggcctac
     1501 aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc
     1561 gtggataagt ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata
     1621 taagtgctgt tccctggtgc ttcctcgctc actcgaaatt cccggggata gctttatgct
     1681 tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg ggacctctag ggtccccaat
     1741 taattagtaa tataatctat taaaggtcat tcaaaaggtc atccaccgga tcaattcccc
     1801 tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc
     1861 ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc
     1921 aaaccctcac tgatccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2101 caggaaacag ctatgaccat gattacgcca agcgcggtac ctgtcgcggc aacgcgctaa
     2161 cagacgtagt aagaaccacc agcattgtaa tgctggctaa agtcactttc ctgagctgta
     2221 taacgatgag cgattttact ttttctggct atgaattggc ctgctttgta acacactccg
     2281 gtctatcccg tagcgccggg catatcctgt cgcaatgtgc aaatctcgcg gcaacaacca
     2341 gtgaatactt cattcacaag cctcaccgcc tgatcgcggc agaaactggt tatagccaat
     2401 caaccgtcgt tcgtgcattc cgtgaagctg taaacaaagg tatcctgtct gtagagattg
     2461 ttatcggcga tcaccgtgaa cgtcgcgcta acctgtaccg gtttacacca tcctttttgg
     2521 ccttcgcaca acaagccaaa aatgcgctga tagaaagcaa attaaagatc tcttcagcgg
     2581 caaccaaggt taaagctgtt ctcgctaaga cattggcttt atttaatttt ttatccacac
     2641 ccccatgtca aaatgatacc ccctccccct gtcaggatga cgtggcaata aagaataaga
     2701 agtcacaagt taaaaaaaca aaaagatcag tttccggcgg tgccggaaca accagcctca
     2761 aaaaattgac ttcatggatc gctaaggcaa aagcaaaggc tgacaatctg cggttatcca
     2821 aaaaacgcac tcaaaaacat gagttcaagc agaaagtaga ggcggctgcg cggaaatatg
     2881 cttacctgaa gaacaagcgt tcgcctgata ttggcgggat atcaaacttc gataacctac
     2941 cgcattgcat gacggtaaac gaagctctta atgcggtttt agccaaaaat aaagataacg
     3001 aacaatgggg tataccggca ggattcagag ggtaatgaat tgctctaatt ataaccatgc
     3061 atactttcaa cacctctagt ttgccatgag gcaaactcat aggtgtcctg gtaagaggac
     3121 actgttgcca aaactggacg ccccattatt gcaattaata aacaactaac ggacaattct
     3181 acctaacaat aagtggagtt gcggccggcc gcgatccgct agcaaagcca cgttgtgtct
     3241 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     3301 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     3361 tgctcgcgcc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3421 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3481 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3541 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3601 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     3661 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3721 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc
     3781 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3841 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactgtt gccattctca
     3901 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3961 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     4021 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     4081 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     4141 tttttctaat cagaattggt taattggttg taacactggc gagctctgcc acctgacgtc
     4201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     4261 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctgcacctg caccagtcag
     4321 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg
     4381 agtgggagAA GAATTACTGA CCCCTCGGGG ATCCATCCAG AGCAATGATG CGTTGCGTTG
     4441 GGATCGGAAG TAGAGATTTC GTAGAGGGAT TATCCGGTGC AACATGGGTT GATGTTGTCC
     4501 TGGAGCATGG GTCTTGTGTC ACAACGATGG CGAAAGATAA GCCTACGTTA GATATAGAGC
     4561 TTCTTAAAAC AGAAGTTACA AACCCAGCTG TGCTAAGGAA ATTATGCATA GAGGCCAAAA
     4621 TCTCCAACAC CACCACGGAC AGTCGTTGCC CCACTCAGGG AGAGGCTACC CTGGTGGAGG
     4681 AGCAAGATGC GAATTTTGTG TGCAGAAGAA CATTCGTCGA TAGAGGTTGG GGGAACGGTT
     4741 GCGGCCTGTT TGGCAAGGGG TCTTTAATTA CATGCGCCAA GTTCAAATGT GTGACTAAAT
     4801 TGGAGGGAAA GATTGTGCAG TATGAAAACC TGAAGTATAG CGTCATTGTA ACGGTTCATA
     4861 CAGGGGACCA ACACCAGGTT GGCAACGAGA GTACAGAGCA CGGTACTACA GCTACTATCA
     4921 CACCCCAGGC CCCAACCACC GAGATACAAC TAACAGACTA CGGCGCTCTG ACACTTGACT
     4981 GCAGCCCCAG GACGGGCTTG GATTTCAATG AGATGGTATT ATTGACCATG AAAGAGAAAA
     5041 GCTGGTTGGT TCATAAACAG TGGTTTCTGG ATCTGCCTTT ACCATGGACT AGCGGCGCCT
     5101 CAACAAGCCA AGAGACTTGG AATCGTCAGG ACCTGTTAGT CACGTTTAAG ACAGCACACG
     5161 CGAAAAAACA GGAAGTGGTT GTCTTAGGCT CCCAAGAGGG AGCGATGCAT ACGGCCTTGA
     5221 CAGGAGCGAC TGAAATTCAG ACTTCCGGCA CCACGACAAT CTTCGCGGGT CATTTGAAGT
     5281 GTAGGTTGAA AATGGATAAA TTGACATTAA AGGGTATGTC ATATGTAATG TGCACCGGTT
     5341 CATTCAAGCT AGAGAAGGAG GTAGCCGAAA CACAACACGG GACTGTGTTA GTACAGATCA
     5401 AGTATGAGGG AACCGACGCT CCATGCAAAA TACCATTTTC TACACAAGAC GAGAAAGGCG
     5461 TCACGCAAAA TGGGCGTTTA ATCACAGCTA ATCCGATTGT GACTGACAAA GAGAAGCCAG
     5521 TAAACATCGA AGCCGAACCG CCCTTTGGGG AAAGTTATAT TGTCATTGGT GCCGGAGAAA
     5581 AAGCTCTGAA GCTGTCCTGG TTTAAGAAGG GTTCAAGCAT TGGGAAGATG TTCGAGGCGA
     5641 CCGCGAGGGG CGCGAGGCGT ATGGCCATTC TTGGCGATAC CGCTTGGGAC TTTGGCTCTA
     5701 TCGGCGGCGT ATTCACTAGC GTGGGAAAGT TGGTTCACCA AATTTTTGGC ACAGCGTATG
     5761 GGGTCCTTTT CAGCGGCGTT TCTTGGACAA TGAAGATAGG CATCGGTGTC CTGCTGACGT
     5821 GGCTTGGATT GAATAGTAGG AGTACAAGTT TGAGTATGAC TTGTATCGCG GTGGGGCTGG
     5881 TTACGCTGTA TCTGGGTGTA ATGGTACAAC GCTGAAGAGG TGCTTCGAGA CCATGTCGAC
     5941 ATACTGTAAG AACCACGCGG GCTAATCGTG GGAGCCTGGC GTAGGGATAA CAGGGTAATT
     6001 TAGCCAGGGT AAGTCAATTA
//