LOCUS       BBF10K_000593           5591 bp ds-DNA     circular     10-AUG-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     source          1..4388
                     /label="source"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      38..85
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    265..515
                     /label="incC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(518..1273)
                     /label="repE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     exon            complement(518..1273)
                     /label="Translation 1273-518"
     rep_origin      1364..1583
                     /label="ori2"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    2003..2024
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        2039..2069
                     /label="lac promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    2077..2093
                     /label="lac operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     2101..2117
                     /label="M13 rev"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2190..3035
                     /label="repL"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     exon            2190..3035
                     /label="Translation 2190-3035"
     misc_feature    3143..3294
                     /label="nnpp promoters"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3334..4149
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            3334..4149
                     /label="Translation 3334-4149"
     terminator      complement(4259..4302)
                     /label="bacterial terminator"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     4320..4336
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      4337..4368
                     /label="tonB terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4389..4408
                     /label="FG Dialout primer FWD"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4409..4414
                     /label="BamHI RE site"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4417..4422
                     /label="BsaI Recognition site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4424..4427
                     /label="BsaI Cut site/MoClo overhang C"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4428..5483
                     /label="yD2NS1 CDS"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5484..5486
                     /label="STOP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5489..5492
                     /label="MoClo overhang D"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5493..5496
                     /label="BsaI cut site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5498..5503
                     /label="BasI Recognition Site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5506..5511
                     /label="SalI RE site"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    5512..5531
                     /label="FG Dialout primer REV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5532..5591
                     /label="FreeGenes Barcode"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5553..5570
                     /label="I-SceI RE site"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc
       61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt
      121 gtcgcgtatg catcgatcac gtgtccacgt tctttaatag tggactcttg ttccaaactg
      181 gaacaacact cggatcgatc cggcgcgcac cgtgggaaaa actccaggta gaggtacaca
      241 cgcggatagc caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa
      301 ctaacccccg atatcaggtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa
      361 ctgccctcaa atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc
      421 aggctgaagg aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga
      481 cgaaccaccc tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga
      541 tatcgcggaa ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg
      601 agaggcgcat tggagttctg ctgttgatct cattaacaca gacttgcagg aagcggcggc
      661 ggaagtcagg catacgctgg taactttgag gcagctggta acgctctatg atccagtcga
      721 ttttcagaga cacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata
      781 aacgcatggc atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc
      841 ggttctgtaa cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc
      901 ctctggatgg actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat
      961 caccggcatc ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg
     1021 cctgccgtat atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag
     1081 caacatggat ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct
     1141 ggtcaacgaa cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag
     1201 tgaggtcgtt tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg
     1261 ctgtttccgc catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa
     1321 taaaaaagag tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat
     1381 ttgtcacagg gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc
     1441 acactgaaag ggcaatttgt cacaacacct tctgtagaac cagcatggat aaaggcctac
     1501 aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc
     1561 gtggataagt ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata
     1621 taagtgctgt tccctggtgc ttcctcgctc actcgaaatt cccggggata gctttatgct
     1681 tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg ggacctctag ggtccccaat
     1741 taattagtaa tataatctat taaaggtcat tcaaaaggtc atccaccgga tcaattcccc
     1801 tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc
     1861 ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc
     1921 aaaccctcac tgatccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     2041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     2101 caggaaacag ctatgaccat gattacgcca agcgcggtac ctgtcgcggc aacgcgctaa
     2161 cagacgtagt aagaaccacc agcattgtaa tgctggctaa agtcactttc ctgagctgta
     2221 taacgatgag cgattttact ttttctggct atgaattggc ctgctttgta acacactccg
     2281 gtctatcccg tagcgccggg catatcctgt cgcaatgtgc aaatctcgcg gcaacaacca
     2341 gtgaatactt cattcacaag cctcaccgcc tgatcgcggc agaaactggt tatagccaat
     2401 caaccgtcgt tcgtgcattc cgtgaagctg taaacaaagg tatcctgtct gtagagattg
     2461 ttatcggcga tcaccgtgaa cgtcgcgcta acctgtaccg gtttacacca tcctttttgg
     2521 ccttcgcaca acaagccaaa aatgcgctga tagaaagcaa attaaagatc tcttcagcgg
     2581 caaccaaggt taaagctgtt ctcgctaaga cattggcttt atttaatttt ttatccacac
     2641 ccccatgtca aaatgatacc ccctccccct gtcaggatga cgtggcaata aagaataaga
     2701 agtcacaagt taaaaaaaca aaaagatcag tttccggcgg tgccggaaca accagcctca
     2761 aaaaattgac ttcatggatc gctaaggcaa aagcaaaggc tgacaatctg cggttatcca
     2821 aaaaacgcac tcaaaaacat gagttcaagc agaaagtaga ggcggctgcg cggaaatatg
     2881 cttacctgaa gaacaagcgt tcgcctgata ttggcgggat atcaaacttc gataacctac
     2941 cgcattgcat gacggtaaac gaagctctta atgcggtttt agccaaaaat aaagataacg
     3001 aacaatgggg tataccggca ggattcagag ggtaatgaat tgctctaatt ataaccatgc
     3061 atactttcaa cacctctagt ttgccatgag gcaaactcat aggtgtcctg gtaagaggac
     3121 actgttgcca aaactggacg ccccattatt gcaattaata aacaactaac ggacaattct
     3181 acctaacaat aagtggagtt gcggccggcc gcgatccgct agcaaagcca cgttgtgtct
     3241 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     3301 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     3361 tgctcgcgcc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     3421 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     3481 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     3541 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     3601 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     3661 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     3721 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc
     3781 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3841 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactgtt gccattctca
     3901 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3961 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     4021 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     4081 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     4141 tttttctaat cagaattggt taattggttg taacactggc gagctctgcc acctgacgtc
     4201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat
     4261 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctgcacctg caccagtcag
     4321 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg
     4381 agtgggagAA GAATTACTGA CCCCTCGGGG ATCCATCCAG AGCAATGGAT AGTGGTTGCG
     4441 TAGTGAGTTG GAAAAACAAA GAATTGAAAT GCGGAAGCGG TATCTTTATC ACCGATAACG
     4501 TACATACTTG GACCGAACAG TATAAATTTC AGCCAGAGTC TCCCTCTAAG CTAGCCTCTG
     4561 CCATCCAAAA GGCGCACGAG GAAGGGATCT GCGGGATCAG GAGCGTCACC AGGCTAGAGA
     4621 ATCTGATGTG GAAGCAGATC ACGCCTGAGC TAAATCACAT CCTGAGTGAG AATGAAGTGA
     4681 AGTTAACAAT TATGACGGGC GACATTAAAG GAATTATGCA GGCGGGCAAG AGGTCTTTAC
     4741 GTCCGCAACC TACAGAACTG AAGTACTCTT GGAAAACTTG GGGAAAGGCC AAGATGCTGT
     4801 CTACGGAAAG CCATAATCAA ACTTTTTTAA TCGATGGACC AGAAACCGCA GAGTGCCCAA
     4861 ACACGAATAG AGCTTGGAAT TCCCTAGAGG TTGAAGACTA TGGTTTCGGA GTTTTTACTA
     4921 CTAACATATG GCTAAAGCTG AGAGAAAAGC AAGACGTGTT CTGCGATTCC AAACTTATGT
     4981 CTGCGGCCAT TAAAGACAAC AGAGCGGTTC ATGCAGACAT GGGTTATTGG ATCGAGTCTG
     5041 CTCTGAATGA CACATGGAAA ATGGAAAAGG CCTCTTTTAT TGAGGTTAAA TCTTGTCACT
     5101 GGCCGAAATC TCATACTCTT TGGAGTAACG GGGTACTTGA GAGCGAAATG ATAATCCCGA
     5161 AAAATTTCGC GGGCCCAGTG AGCCAGCATA ACTATCGTCC CGGTTATCAT ACTCAAACAG
     5221 CCGGACCGTG GCATCTGGGA AAGTTGGAGA TGGACTTTGA TTTCTGCGAA GGAACAACAG
     5281 TTGTGGTTAC CGAAGACTGC GGGAACAGGG GGCCTTCTCT AAGAACGACA ACTGCGAGTG
     5341 GCAAACTTAT AACCGAGTGG TGCTGTAGAT CTTGTACGTT GCCACCCTTG AGATATAGAG
     5401 GAGAAGATGG CTGCTGGTAC GGCATGGAGA TAAGACCCCT AAAGGAAAAA GAGGAAAATC
     5461 TGGTTAACTC CCTAGTGACA GCTTGAAGAG GTGCTTCGAG ACCATGTCGA CATCACAACA
     5521 AAGGACGGGT CAAGGATATA CTACATTGAA TTTAGGGATA ACAGGGTAAT TATTGCCACA
     5581 GCCCCATTCC C
//