LOCUS BBF10K_000598 6047 bp ds-DNA circular 10-AUG-2022 DEFINITION . FEATURES Location/Qualifiers source 1..4388 /label="source" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd terminator 38..85 /label="T7 terminator" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind complement(90..106) /label="M13 rev" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 265..515 /label="incC" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS complement(518..1273) /label="repE" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 exon complement(518..1273) /label="Translation 1273-518" rep_origin 1364..1583 /label="ori2" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 protein_bind 2003..2024 /label="CAP binding site" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc promoter 2039..2069 /label="lac promoter" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac protein_bind 2077..2093 /label="lac operator" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind 2101..2117 /label="M13 rev" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 CDS 2190..3035 /label="repL" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac exon 2190..3035 /label="Translation 2190-3035" misc_feature 3143..3294 /label="nnpp promoters" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 3334..4149 /label="KanR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 exon 3334..4149 /label="Translation 3334-4149" terminator complement(4259..4302) /label="bacterial terminator" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind 4320..4336 /label="M13 fwd" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 terminator 4337..4368 /label="tonB terminator" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 4389..4408 /label="FG Dialout Primer FWD" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 4409..4414 /label="BamHI RE site" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 4417..4422 /label="BsaI Recognition site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 4424..4427 /label="BsaI Cut site/MoClo overhang C" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 4428..5939 /label="ZE CDS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 5940..5942 /label="STOP" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 5945..5948 /label="MoClo overhang D" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 5949..5952 /label="BsaI cut site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 5954..5959 /label="BasI Recognition Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 5962..5967 /label="SalI RE site" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 5968..5987 /label="FG Dialout Primer REV" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 5988..6047 /label="FreeGenes Barcode" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 6009..6026 /label="I-SceI RE site" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd ORIGIN 1 cgctgaggtg tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc 61 taaacgggtc ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt 121 gtcgcgtatg catcgatcac gtgtccacgt tctttaatag tggactcttg ttccaaactg 181 gaacaacact cggatcgatc cggcgcgcac cgtgggaaaa actccaggta gaggtacaca 241 cgcggatagc caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa 301 ctaacccccg atatcaggtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa 361 ctgccctcaa atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc 421 aggctgaagg aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga 481 cgaaccaccc tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga 541 tatcgcggaa ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg 601 agaggcgcat tggagttctg ctgttgatct cattaacaca gacttgcagg aagcggcggc 661 ggaagtcagg catacgctgg taactttgag gcagctggta acgctctatg atccagtcga 721 ttttcagaga cacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata 781 aacgcatggc atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc 841 ggttctgtaa cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc 901 ctctggatgg actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat 961 caccggcatc ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg 1021 cctgccgtat atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag 1081 caacatggat ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct 1141 ggtcaacgaa cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag 1201 tgaggtcgtt tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg 1261 ctgtttccgc catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa 1321 taaaaaagag tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat 1381 ttgtcacagg gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc 1441 acactgaaag ggcaatttgt cacaacacct tctgtagaac cagcatggat aaaggcctac 1501 aaggcgctct aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc 1561 gtggataagt ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata 1621 taagtgctgt tccctggtgc ttcctcgctc actcgaaatt cccggggata gctttatgct 1681 tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg ggacctctag ggtccccaat 1741 taattagtaa tataatctat taaaggtcat tcaaaaggtc atccaccgga tcaattcccc 1801 tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc cttggagccc 1861 ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac ccgcagttgc 1921 aaaccctcac tgatccgatt cattaatgca gctggcacga caggtttccc gactggaaag 1981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 2041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 2101 caggaaacag ctatgaccat gattacgcca agcgcggtac ctgtcgcggc aacgcgctaa 2161 cagacgtagt aagaaccacc agcattgtaa tgctggctaa agtcactttc ctgagctgta 2221 taacgatgag cgattttact ttttctggct atgaattggc ctgctttgta acacactccg 2281 gtctatcccg tagcgccggg catatcctgt cgcaatgtgc aaatctcgcg gcaacaacca 2341 gtgaatactt cattcacaag cctcaccgcc tgatcgcggc agaaactggt tatagccaat 2401 caaccgtcgt tcgtgcattc cgtgaagctg taaacaaagg tatcctgtct gtagagattg 2461 ttatcggcga tcaccgtgaa cgtcgcgcta acctgtaccg gtttacacca tcctttttgg 2521 ccttcgcaca acaagccaaa aatgcgctga tagaaagcaa attaaagatc tcttcagcgg 2581 caaccaaggt taaagctgtt ctcgctaaga cattggcttt atttaatttt ttatccacac 2641 ccccatgtca aaatgatacc ccctccccct gtcaggatga cgtggcaata aagaataaga 2701 agtcacaagt taaaaaaaca aaaagatcag tttccggcgg tgccggaaca accagcctca 2761 aaaaattgac ttcatggatc gctaaggcaa aagcaaaggc tgacaatctg cggttatcca 2821 aaaaacgcac tcaaaaacat gagttcaagc agaaagtaga ggcggctgcg cggaaatatg 2881 cttacctgaa gaacaagcgt tcgcctgata ttggcgggat atcaaacttc gataacctac 2941 cgcattgcat gacggtaaac gaagctctta atgcggtttt agccaaaaat aaagataacg 3001 aacaatgggg tataccggca ggattcagag ggtaatgaat tgctctaatt ataaccatgc 3061 atactttcaa cacctctagt ttgccatgag gcaaactcat aggtgtcctg gtaagaggac 3121 actgttgcca aaactggacg ccccattatt gcaattaata aacaactaac ggacaattct 3181 acctaacaat aagtggagtt gcggccggcc gcgatccgct agcaaagcca cgttgtgtct 3241 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg 3301 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct 3361 tgctcgcgcc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 3421 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg 3481 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 3541 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 3601 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta 3661 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 3721 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc 3781 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 3841 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactgtt gccattctca 3901 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 3961 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 4021 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 4081 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 4141 tttttctaat cagaattggt taattggttg taacactggc gagctctgcc acctgacgtc 4201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat 4261 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctgcacctg caccagtcag 4321 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg 4381 agtgggagAA GAATTACTGA CCCCTCGGGG ATCCATCCAG AGCAATGATA AGGTGTATCG 4441 GAGTTAGCAA TCGTGATTTT GTGGAGGGCA TGTCTGGTGG AACTTGGGTC GACGTAGTGC 4501 TAGAACATGG GGGGTGTGTG ACGGTTATGG CGCAAGACAA ACCTACTGTT GACATCGAGC 4561 TTGTCACTAC AACAGTGTCT AACATGGCAG AAGTCCGTTC CTATTGCTAT GAGGCATCCA 4621 TTTCCGACAT GGCGTCCGAT AGTCGTTGTC CTACCCAAGG CGAAGCTTAT CTAGATAAAC 4681 AATCAGATAC GCAGTATGTT TGTAAAAGGA CGTTGGTTGA TAGGGGTTGG GGCAACGGGT 4741 GTGGACTTTT TGGGAAGGGA TCTTTAGTTA CGTGCGCCAA ATTCGCGTGT AGCAAGAAAA 4801 TGACTGGCAA ATCTATACAA CCGGAAAATC TTGAGTACAG GATAATGCTT TCTGTGCACG 4861 GTTCTCAACA CAGTGGAATG ATCGTAAACG ATACCGGTCA TGAGACAGAC GAAAATAGGG 4921 CAAAAGTGGA AATAACTCCC AACAGCCCGA GGGCTGAAGC CACATTAGGT GGGTTCGGCA 4981 GCCTAGGTCT TGACTGTGAA CCCAGAACTG GGTTAGACTT TTCCGACTTG TACTACCTAA 5041 CGATGAACAA CAAACATTGG TTAGTGCATA AAGAGTGGTT TCACGACATT CCTTTACCTT 5101 GGCATGCGGG CGCGGATACT GGCACTCCCC ATTGGAATAA TAAGGAAGCC CTAGTAGAGT 5161 TCAAAGACGC GCACGCGAAG AGGCAGACGG TAGTGGTATT AGGCAGTCAG GAAGGGGCTG 5221 TGCACACTGC TCTGGCTGGC GCACTAGAAG CTGAGATGGA CGGCGCCAAA GGTCGTTTGT 5281 CAAGCGGGCA TTTGAAATGC AGACTAAAAA TGGATAAGTT ACGTCTGAAG GGCGTGAGTT 5341 ATTCCCTGTG CACCGCGGCT TTCACCTTTA CAAAAATACC GGCGGAAACG CTGCACGGAA 5401 CAGTAACGGT CGAAGTGCAA TATGCCGGGA CCGACGGTCC ATGCAAGGTG CCTGCCCAAA 5461 TGGCGGTCGA TATGCAAACG CTGACACCGG TGGGTCGTCT AATAACCGCC AACCCAGTGA 5521 TCACCGAAAG TACAGAGAAT TCTAAGATGA TGCTAGAACT AGATCCCCCA TTTGGCGACA 5581 GCTATATCGT GATCGGCGTG GGCGAGAAAA AAATAACACA CCACTGGCAC CGTAGCGGGT 5641 CAACGATCGG AAAGGCCTTC GAAGCAACGG TCAGAGGGGC TAAGAGGATG GCAGTACTGG 5701 GGGATACAGC CTGGGACTTT GGAAGCGTCG GGGGAGCACT AAACTCTTTA GGAAAAGGAA 5761 TACACCAAAT CTTCGGGGCA GCTTTCAAAA GTTTATTTGG TGGTATGTCC TGGTTCTCTC 5821 AAATATTGAT CGGGACGCTG CTAATGTGGT TGGGCTTGAA TACAAAGAAT GGAAGTATCT 5881 CTCTGATGTG CCTTGCCTTA GGAGGAGTTC TTATTTTCTT GAGCACGGCG GTCTCTGCGT 5941 GAAGAGGTGC TTCGAGACCA TGTCGACATG CTAGCTGGAA CTATCGGCCC TGTTCTAACT 6001 TTTTGGGTTA GGGATAACAG GGTAATAAAG GTATGATTTC GTACGAC //