LOCUS       Exported     3940 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3940
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..3926
                     /label="gyrB"
                     /note="A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner."
                     /translation="MEQQQNSYDENQIQVLEGLEAVRKRPGMYIGSTNSKGLHHLVWEIVDNSIDEALAGYCTDINIQIEKDNSITVVDNGRGIPVGIHEKMGRPAVEVIMTVLHAGGKFDGSGYKVSGGLHGVGASVVNALSTELDVTVHRDGKIHRQTYKRGVPVTDLEIIGETDHTGTTTHFVPDPEIFSETTEYDYDLLANRVRELAFLTKGVNITIEDKREGQERKNEYHYEGGIKSYVEYLNRSKEVVHEEPIYIEGEKDGITVEVALQYNDSYTSNIYSFTNNINTYEGGTHEAGFKTGLTRVINDYARKKGLIKENDPNLSGDDVREGLTAIISIKHPDPQFEGQTKTKLGNSEARTITDTLFSTAMETFMLENPDAAKKIVDKGLMAARARMAAKKARELTRRKSALEISNLPGKLADCSSKDPSISELYIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILSNNEVRSMITALGTGIGEDFNLEKARYHKVVIMTDADVDGAHIRTLLLTFFYRYMRQIIENGYVYIAQPPLYKVQQGKRVEYAYNDKELEELLKTLPQTPKPGLQRYKGLGEMNATQLWETTMDPSSRTLLQVTLEDAMDADETFEMLMGDKVEPRRNFIEANARYVKNLDI"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGGAACAACA GCAGAATAGC TACGACGAAA
     2041 ATCAAATTCA AGTCCTGGAG GGTCTGGAGG CGGTCCGCAA GCGCCCGGGT ATGTACATTG
     2101 GTAGCACCAA CTCAAAGGGT CTGCACCATC TGGTCTGGGA AATCGTCGAT AATTCCATTG
     2161 ACGAAGCGTT GGCGGGTTAT TGTACCGACA TTAATATCCA GATTGAGAAG GATAACTCGA
     2221 TCACCGTGGT CGATAATGGT CGTGGCATCC CGGTGGGTAT CCACGAAAAG ATGGGTCGCC
     2281 CGGCTGTGGA GGTGATCATG ACCGTGCTGC ACGCTGGTGG CAAATTCGAC GGTAGCGGTT
     2341 ATAAAGTGTC GGGCGGTCTG CATGGTGTTG GTGCGAGCGT GGTTAATGCG CTGTCCACCG
     2401 AGCTGGATGT GACCGTTCAC CGTGACGGCA AAATCCATCG TCAGACGTAT AAGCGCGGCG
     2461 TGCCGGTGAC CGACCTGGAG ATTATTGGTG AAACCGACCA CACTGGTACC ACCACGCATT
     2521 TTGTGCCGGA TCCGGAAATT TTTAGCGAGA CTACGGAATA CGACTACGAT CTGCTGGCGA
     2581 ATCGTGTGCG TGAACTGGCT TTCCTGACCA AAGGCGTGAA CATCACGATC GAGGATAAAC
     2641 GTGAAGGCCA AGAGCGTAAA AACGAGTATC ACTATGAGGG TGGCATTAAA TCCTACGTCG
     2701 AGTACCTGAA CCGTTCTAAG GAGGTTGTGC ACGAGGAGCC AATCTACATT GAGGGTGAAA
     2761 AGGATGGTAT TACCGTTGAA GTTGCGCTGC AATACAACGA TTCGTATACG AGCAACATCT
     2821 ATAGCTTCAC CAACAATATT AATACCTATG AGGGTGGTAC GCACGAAGCG GGTTTTAAGA
     2881 CGGGCCTGAC CCGTGTCATC AATGACTATG CCCGTAAGAA AGGTCTGATT AAGGAGAATG
     2941 ACCCGAACTT GTCCGGTGAC GACGTCCGTG AGGGTCTGAC TGCCATCATT TCAATCAAGC
     3001 ATCCGGATCC GCAGTTTGAG GGTCAGACCA AAACGAAGTT GGGTAACTCT GAGGCACGTA
     3061 CCATTACCGA CACCCTGTTT TCAACCGCAA TGGAAACCTT CATGTTGGAA AACCCGGACG
     3121 CAGCGAAGAA AATTGTGGAT AAAGGTTTGA TGGCTGCACG TGCCAGAATG GCGGCGAAGA
     3181 AAGCGCGTGA ACTGACCAGA CGTAAAAGCG CATTGGAAAT TAGCAACTTG CCGGGTAAAC
     3241 TGGCGGATTG CTCGAGCAAG GATCCGTCCA TCAGCGAGCT GTACATCGTT GAGGGTGACA
     3301 GCGCCGGTGG TTCCGCAAAA CAGGGTCGTG ATCGTCATTT TCAAGCGATC TTGCCGCTGC
     3361 GTGGCAAGAT CCTGAATGTG GAAAAGGCGC GCCTGGATAA AATTCTGAGC AATAACGAGG
     3421 TGCGCAGCAT GATCACGGCG CTGGGTACCG GCATCGGTGA AGATTTTAAT CTGGAAAAGG
     3481 CCCGTTACCA CAAAGTCGTG ATCATGACTG ATGCCGACGT TGACGGTGCT CACATCCGTA
     3541 CCTTGCTGCT GACCTTCTTT TACCGTTATA TGCGTCAGAT CATCGAGAAC GGCTATGTTT
     3601 ATATCGCGCA GCCACCGCTG TACAAAGTCC AACAGGGTAA GCGTGTGGAG TACGCGTACA
     3661 ACGACAAAGA GCTGGAGGAG CTGCTGAAAA CCTTGCCGCA GACCCCGAAG CCGGGTCTGC
     3721 AACGCTATAA GGGCCTGGGT GAGATGAACG CCACCCAGCT GTGGGAAACC ACTATGGATC
     3781 CGAGCTCCCG TACCCTGCTT CAGGTAACGC TGGAGGACGC TATGGATGCG GATGAAACGT
     3841 TCGAGATGTT GATGGGTGAC AAAGTTGAAC CGCGTCGTAA TTTTATTGAG GCGAATGCAC
     3901 GCTACGTTAA GAACCTGGAT ATTTGAAGAG CTTAGAGACC
//