LOCUS       Exported     4489 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4489
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2010..4475
                     /translation="MSEQNTPQVREINISQEMRTSFLDYAMSVIVSRALPDVRDGLKPVHRRILYAMNDLGMTSDKPYKKSARIVGEVIGKYHPHGDSAVYESMVRMAQDFNYRYMLVDGHGNFGSVDGDSAAAMRYTEARMSKISMEILRDITKDTIDYQDNYDGSEREPVVMPSRFPNLLVNGAAGIAVGMATNIPPHQLGEIIDGVLAVSENPDITIPELMEVIPGPDFPTAGQILGRSGIRKAYESGRGSITIRAKAEIEQTSSGKERIIVTELPYQVNKAKLIEKIADLVRDKKIEGITDLRDESDRTGMRIVIEIRRDANANVILNNLYKQTALQTSFGINLLALVDGQPKVLTLKQCLEHYLDHQKVVIRRRTAYELRKAEARAHILEGLRVALDHLDAVISLIRNSQTAEIARTGLIEQFSLTEKQAQAILDMRLQRLTGLEREKIEEEYQSLVKLIAELKDILANEYKVLEIIREELTEIKERFNDERRTEIVTSGLETIEDEDLIERENIVVTLTHNGYVKRLPASTYRSQKRGGKGVQGMGTNEDDFVEHLISTSTHDTILFFSNKGKVYRAKGYEIPEYGRTAKGIPIINLLEVEKGEWINAIIPVTEFNAELYLFFTTKHGVSKRTSLSQFANIRNNGLIALSLREDDELMGVRLTDGTKQIIIGTKNGLLIRFPETDVREMGRTAAGVKGITLTDDDVVVGMEILEEESHVLIVTEKGYGKRTPAEEYRTQSRGGKGLKTAKITENNGQLVAVKATKGEEDLMIITASGVLIRMDINDISITGRVTQGVRLIRMAEEEHVATVALVEKNEEDENEEEQEEV"
                     /label="gyrA"
                     /note="A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GGGTCTCAAA TGAGCGAGCA GAACACCCCT CAGGTTCGTG
     2041 AGATTAACAT CAGCCAGGAG ATGCGTACCA GCTTCCTGGA TTATGCCATG AGCGTGATTG
     2101 TTAGCCGCGC GCTGCCGGAT GTCCGTGACG GCCTGAAACC AGTTCACCGT CGTATCCTGT
     2161 ATGCAATGAA TGATTTGGGC ATGACCTCCG ATAAACCGTA CAAGAAAAGC GCACGTATTG
     2221 TCGGTGAAGT GATTGGTAAA TACCATCCTC ATGGCGACAG CGCGGTTTAT GAGAGCATGG
     2281 TCCGCATGGC GCAGGATTTT AACTACCGTT ATATGCTGGT CGATGGTCAT GGCAATTTTG
     2341 GTAGCGTTGA TGGCGACAGC GCTGCGGCAA TGCGCTATAC GGAAGCGCGC ATGTCGAAGA
     2401 TTAGCATGGA GATTTTACGT GATATCACGA AGGATACTAT CGACTACCAG GATAACTATG
     2461 ATGGCAGCGA GCGTGAGCCG GTTGTGATGC CGAGCCGTTT CCCAAATCTG CTGGTGAACG
     2521 GTGCGGCGGG TATTGCTGTG GGCATGGCCA CCAATATCCC GCCGCACCAG CTGGGTGAAA
     2581 TTATCGACGG CGTGTTGGCA GTCAGCGAGA ACCCTGATAT TACCATCCCG GAGCTCATGG
     2641 AAGTTATTCC GGGCCCGGAC TTTCCAACCG CGGGTCAGAT CCTGGGTCGT AGCGGTATTC
     2701 GTAAAGCGTA CGAGAGCGGT CGCGGTTCAA TTACCATTCG TGCGAAGGCA GAGATCGAGC
     2761 AGACCAGCTC GGGCAAGGAA CGTATTATCG TTACCGAGCT TCCTTACCAG GTTAACAAAG
     2821 CGAAACTGAT CGAGAAAATC GCGGACCTGG TCCGTGACAA GAAAATCGAA GGTATTACCG
     2881 ATCTGCGTGA TGAGAGCGAC CGTACTGGCA TGCGCATCGT GATTGAGATC CGTCGCGACG
     2941 CTAATGCAAA TGTGATTCTC AACAACCTGT ACAAACAGAC GGCCCTTCAG ACGAGCTTTG
     3001 GTATCAACTT GCTGGCACTG GTTGATGGCC AACCGAAGGT CCTGACCCTG AAGCAGTGTC
     3061 TGGAGCATTA CCTGGACCAC CAGAAGGTGG TCATCCGCCG CCGCACCGCG TACGAGCTGC
     3121 GCAAGGCTGA GGCGCGCGCG CACATCCTCG AGGGCTTGCG TGTCGCACTG GACCACCTGG
     3181 ATGCAGTGAT CTCTCTGATC CGCAACAGCC AGACCGCAGA AATCGCGCGT ACCGGCCTGA
     3241 TCGAACAGTT CAGCCTGACC GAAAAACAAG CACAGGCCAT CCTGGATATG CGTCTGCAGC
     3301 GTTTAACGGG TCTGGAGCGC GAGAAAATCG AGGAGGAGTA TCAGAGCCTG GTGAAGCTGA
     3361 TCGCGGAACT GAAGGACATT CTGGCGAACG AATATAAAGT ACTGGAGATT ATTCGTGAAG
     3421 AACTGACTGA AATCAAGGAG CGCTTCAATG ACGAGCGTCG TACCGAGATT GTTACTAGCG
     3481 GTCTGGAAAC CATCGAGGAC GAGGACCTTA TCGAACGCGA AAACATTGTT GTTACGCTGA
     3541 CCCATAATGG TTACGTCAAA CGTTTGCCGG CGTCCACGTA CCGCTCCCAG AAACGTGGTG
     3601 GTAAAGGCGT CCAAGGTATG GGTACCAATG AAGATGACTT TGTGGAGCAC CTGATTAGCA
     3661 CCAGCACCCA CGACACCATT TTGTTCTTCA GCAATAAAGG CAAGGTGTAC CGCGCGAAGG
     3721 GCTACGAGAT CCCGGAATAC GGTCGTACCG CGAAAGGTAT TCCGATCATT AATCTGCTGG
     3781 AGGTCGAGAA GGGCGAGTGG ATCAATGCGA TCATCCCTGT AACCGAGTTC AACGCGGAGC
     3841 TCTATCTGTT CTTCACGACT AAACATGGTG TTTCCAAGCG CACCTCCCTG AGCCAGTTTG
     3901 CGAATATCCG CAACAATGGC CTGATCGCGC TGAGCCTGCG TGAAGATGAT GAGCTGATGG
     3961 GCGTTCGTCT GACCGATGGC ACGAAACAAA TCATTATCGG TACGAAAAAC GGCCTGTTAA
     4021 TTCGCTTCCC GGAAACCGAC GTGCGTGAGA TGGGTCGTAC TGCAGCGGGT GTTAAGGGTA
     4081 TCACCCTGAC GGATGACGAC GTTGTGGTTG GTATGGAGAT CCTGGAGGAA GAGAGCCATG
     4141 TCCTGATCGT GACCGAGAAA GGCTACGGCA AACGTACGCC GGCAGAGGAA TACCGCACGC
     4201 AGAGCCGTGG TGGTAAGGGC TTAAAGACGG CAAAGATCAC CGAAAATAAC GGCCAGCTGG
     4261 TCGCGGTTAA AGCGACCAAG GGCGAAGAAG ATCTGATGAT TATCACGGCG AGCGGTGTTC
     4321 TGATCCGCAT GGACATCAAT GACATCAGCA TTACGGGCAG AGTTACCCAG GGTGTTCGTC
     4381 TGATCCGCAT GGCAGAAGAG GAGCACGTCG CAACCGTTGC GCTGGTTGAG AAAAACGAGG
     4441 AAGATGAGAA TGAGGAGGAG CAAGAGGAGG TGTGAAGAGC TTAGAGACC
//